##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527903_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	373883
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.298205588379254	33.0	31.0	34.0	31.0	34.0
2	32.98748538981446	34.0	31.0	34.0	31.0	34.0
3	33.28403003078503	34.0	34.0	34.0	31.0	34.0
4	36.508092103679495	37.0	37.0	37.0	35.0	37.0
5	36.413412752117644	37.0	37.0	37.0	35.0	37.0
6	36.514043163235556	37.0	37.0	37.0	35.0	37.0
7	36.52328669664039	37.0	37.0	37.0	35.0	37.0
8	36.644656215982	37.0	37.0	37.0	35.0	37.0
9	38.76887956927702	39.0	39.0	39.0	39.0	39.0
10	38.32810264173552	39.0	39.0	39.0	37.0	39.0
11	38.10440431899819	39.0	39.0	39.0	37.0	39.0
12	37.639205312892	39.0	37.0	39.0	35.0	39.0
13	37.52714886742644	39.0	37.0	39.0	35.0	39.0
14	38.340288271999526	40.0	38.0	40.0	35.0	40.0
15	38.3764707141004	40.0	38.0	40.0	35.0	40.0
16	38.46303522759794	40.0	38.0	40.0	35.0	40.0
17	38.41900541078359	40.0	38.0	40.0	35.0	40.0
18	38.31529382186406	40.0	38.0	40.0	35.0	40.0
19	38.338958979145886	40.0	38.0	40.0	35.0	40.0
20	38.2523169012766	40.0	38.0	40.0	34.0	40.0
21	38.19778379867498	40.0	38.0	40.0	34.0	40.0
22	38.21971311880989	40.0	38.0	40.0	34.0	40.0
23	38.15646338560459	40.0	38.0	40.0	34.0	40.0
24	38.062428620718244	40.0	38.0	40.0	34.0	40.0
25	38.03983331683976	40.0	38.0	40.0	34.0	40.0
26	37.89948994738996	40.0	37.0	40.0	34.0	40.0
27	37.806361883262944	40.0	37.0	40.0	34.0	40.0
28	37.608208985163806	40.0	37.0	40.0	33.0	40.0
29	37.64892493106132	40.0	37.0	40.0	34.0	40.0
30	37.752443946368246	40.0	37.0	40.0	34.0	40.0
31	37.786901249856236	40.0	37.0	40.0	34.0	40.0
32	37.726296729190686	40.0	37.0	40.0	34.0	40.0
33	37.64506543490878	40.0	36.0	40.0	34.0	40.0
34	37.293000751572016	40.0	35.0	40.0	33.0	40.0
35	37.23244704894312	40.0	35.0	40.0	33.0	40.0
36	37.25010230473169	40.0	35.0	40.0	33.0	40.0
37	37.16015170521259	40.0	35.0	40.0	33.0	40.0
38	37.04437216990342	40.0	35.0	40.0	33.0	40.0
39	36.96771717355429	40.0	35.0	40.0	33.0	40.0
40	36.773284690665264	39.0	35.0	40.0	33.0	40.0
41	36.60900067668228	39.0	35.0	40.0	32.0	40.0
42	36.50452681721287	39.0	35.0	40.0	32.0	40.0
43	36.38765603143229	39.0	35.0	40.0	32.0	40.0
44	36.04523340189311	38.0	35.0	40.0	31.0	40.0
45	36.032865896550526	38.0	35.0	40.0	31.0	40.0
46	35.843333342248776	38.0	35.0	40.0	31.0	40.0
47	35.6781533260405	37.0	35.0	40.0	30.0	40.0
48	35.62728447134531	37.0	35.0	40.0	30.0	40.0
49	35.385679477269626	37.0	35.0	40.0	30.0	40.0
50	35.00798645565592	36.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	4.0
16	18.0
17	24.0
18	55.0
19	80.0
20	151.0
21	273.0
22	427.0
23	592.0
24	835.0
25	1160.0
26	1489.0
27	2064.0
28	2797.0
29	3567.0
30	4604.0
31	6017.0
32	7522.0
33	10271.0
34	20830.0
35	26443.0
36	33143.0
37	55738.0
38	115264.0
39	80514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.38670653653683	0.40948639012739274	0.07997154189947123	0.12383553143630495
8	99.44902549728124	0.41563804719658287	0.10885758378958123	0.02647887173260084
9	99.12058050245665	0.5116573901461152	0.16101293720227985	0.20674917019495404
10	65.83423156441988	25.854612271753464	3.2194563539930945	5.091699809833558
11	37.12578533926389	28.03631082450927	17.45599559220398	17.381908244022863
12	29.88234287196797	18.337554796554002	26.25019056763747	25.529911763840555
13	24.305999470422567	16.914382306764416	30.996060264842214	27.78355795797081
14	24.373400234832822	18.91581056105787	30.67644156059516	26.03434764351415
15	22.675007957034687	19.86958487013317	29.02512283254387	28.43028434028827
16	28.60039103141892	20.8672231687453	23.352760088048935	27.17962571178684
17	30.697838628661906	20.7792277263208	23.312640585423782	25.210293059593507
18	27.298646902908132	21.223216888705824	23.461885135189352	28.016251073196695
19	29.895716039509683	22.104508629705013	21.433978009163294	26.565797321622004
20	29.095198230462472	21.467143464666755	21.99297641240709	27.444681892463684
21	30.39988445583244	20.885678139952873	23.921387171922767	24.793050232291918
22	30.31456364691628	20.454527218407897	22.686776344471397	26.544132790204422
23	28.516140075906097	21.65249556679496	23.56780062211975	26.263563735179186
24	29.487566966136463	22.524961017216615	22.317141993618325	25.670330023028594
25	28.20802229574493	22.914655119382267	21.690207899262603	27.187114685610204
26	27.142448306020867	22.638632941321216	21.901236483070907	28.317682269587007
27	26.629186135769743	21.906853213438428	23.04758440474694	28.416376246044884
28	29.159656898013548	21.602212456838103	20.245370878055436	28.992759767092913
29	29.6373464426037	22.533519844443315	23.118729656068876	24.710404056884105
30	28.9101135916851	20.206053765482785	22.51907682349826	28.364755819333855
31	28.10932831928705	23.064969522551173	21.99217402235459	26.83352813580719
32	31.715536678586613	23.125683703190568	21.680044291930898	23.478735326291915
33	32.757038966735585	21.760550760532038	21.064343658310218	24.41806661442216
34	28.17218220673312	25.856751978560137	23.17008261942907	22.800983195277666
35	27.72016914382307	24.985891308243488	25.744684834560545	21.5492547133729
36	29.54052470960167	23.532227996458786	21.944833009256907	24.98241428468264
37	27.678712324443744	26.03434764351415	21.18898157979903	25.09795845224308
38	31.695744390624874	25.552111221959812	21.122650668792108	21.629493718623205
39	28.859830481728245	24.70585717991992	20.44115405086618	25.993158287485656
40	27.651163599307804	23.96311145465293	22.097019655881653	26.288705290157615
41	27.831968824471826	23.991997496543036	23.492108493833634	24.683925185151505
42	27.025834285057087	23.076202983286215	21.609166503959795	28.2887962276969
43	26.79902536354956	20.985174506463252	25.151718585760786	27.064081544226404
44	26.15417122468794	20.393545574417665	25.376655263812474	28.075627937081922
45	27.371664397685908	20.271314823086367	25.3916332114592	26.965387567768527
46	28.06733657320606	22.931772773835664	22.8627672293204	26.138123423637875
47	26.984912392379435	24.597801986182844	22.56695276329761	25.850332858140114
48	30.025435764664348	23.606582807990737	21.952589446431105	24.415391980913814
49	27.565040400339143	22.852603621988695	24.550460973085166	25.031895004586996
50	25.5061075256163	24.091493863053415	23.424707729423375	26.977690881906906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	5.5
4	11.0
5	18.0
6	25.0
7	15.0
8	5.0
9	79.0
10	153.0
11	776.0
12	1399.0
13	1508.5
14	1618.0
15	1396.5
16	1175.0
17	994.0
18	813.0
19	873.0
20	933.0
21	1045.0
22	1157.0
23	1855.0
24	2553.0
25	2122.5
26	1692.0
27	2009.5
28	2327.0
29	2556.0
30	2785.0
31	3295.0
32	3805.0
33	3836.0
34	3867.0
35	4656.5
36	5446.0
37	6149.5
38	6853.0
39	7659.0
40	8465.0
41	9092.0
42	9719.0
43	10269.5
44	10820.0
45	11914.5
46	13009.0
47	14313.5
48	15618.0
49	16608.0
50	17598.0
51	17871.0
52	18144.0
53	20381.0
54	22618.0
55	28065.5
56	33513.0
57	33067.5
58	32622.0
59	35466.5
60	38311.0
61	35221.0
62	32131.0
63	28823.5
64	25516.0
65	20997.0
66	16478.0
67	14982.5
68	13487.0
69	11928.0
70	10369.0
71	8421.5
72	6474.0
73	6689.0
74	6904.0
75	5174.0
76	3444.0
77	2354.5
78	1265.0
79	874.0
80	483.0
81	338.5
82	194.0
83	116.5
84	39.0
85	39.5
86	40.0
87	22.0
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	373883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.218972245328084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.4610935168948	7.098209867792865
2	8.901510194466983	1.819285712375262
3	3.3711100060198396	1.033478387623936
4	1.617504645745544	0.6611694032625179
5	0.8270735729054886	0.42259209431827605
6	0.5784280367471929	0.35465640320635067
7	0.36904232208757554	0.26398632727350535
8	0.36380767922108515	0.29741924612779935
9	0.31931321485591646	0.2936747592161184
>10	8.461800193681785	25.123634933923178
>50	4.090873400162274	28.694538130912612
>100	1.5651582170806397	26.828446332141336
>500	0.05496375009814955	3.7372654012083992
>1k	0.018321250032716518	3.37164300061784
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3727	0.9968359085596297	No Hit
CAACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2938	0.785807324751326	No Hit
CAACAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	1745	0.46672354720594406	No Hit
CAACAAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	1070	0.2861857853927566	No Hit
CAACAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	1045	0.2794992016218978	No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	1043	0.27896427492022907	No Hit
CAACAAGGGGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGG	1038	0.2776269581660573	No Hit
CAACAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	934	0.24981076967928467	No Hit
CAACAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	846	0.22627399480586174	No Hit
CAACAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	818	0.21878502098249988	No Hit
CAACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	784	0.20969126705413188	No Hit
CAACAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	742	0.19845780631908913	No Hit
CAACAAGGGATGTGTGTATGTATGTGTGTCTGTGTGTGTCTGTGTGTCCA	730	0.1952482461090769	No Hit
CAACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	721	0.19284107595156774	No Hit
CAACAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	717	0.1917712225482303	No Hit
CAACAAGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCG	700	0.18722434558404635	No Hit
CAACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	654	0.17492103144566618	No Hit
CAACAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	632	0.1690368377273104	No Hit
CAACAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	626	0.1674320576223043	No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	610	0.16315264400895468	No Hit
CAACAAGGGAGATTTCTACCACTCCAAAAAAAAAAAAAAAAAAAAAAAAA	608	0.16261771730728597	No Hit
CAACAAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	600	0.16047801050061117	No Hit
CAACAAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA	585	0.15646606023809587	No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	576	0.1540588900805867	No Hit
CAACAAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	571	0.15272157332641495	No Hit
CAACAAGGGGGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	513	0.13720869897802254	No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTG	505	0.1350689921713477	No Hit
CAACAAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGT	501	0.1339991387680103	No Hit
CAACAAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	500	0.13373167541717595	No Hit
CAACAAGGGGACGGGGGTAGCGTGGCCGAGCGGTCTAAGGCGCTGGATTA	499	0.13346421206634163	No Hit
CAACAAGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	490	0.13105704190883244	No Hit
CAACAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG	485	0.12971972515466068	No Hit
CAACAAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	481	0.12864987175132328	No Hit
CAACAAGGGGAGCATCAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGA	480	0.12838240840048892	No Hit
CAACAAGGGGAGCACAGCTGGCCATCAGAGTCACCAATCCCAACGCCAGG	478	0.12784748169882024	No Hit
CAACAAGGGGGAGCTGGTGAGATGGCTCCGCAGTTAAGAGCACTGACTAC	473	0.12651016494464848	No Hit
CAACAAGGGAGTGAGCACACTGCTTCAAATAAGGTCATGTGTGAAAGAAC	471	0.12597523824297976	No Hit
CAACAAGGGTAGCAAATCTACTCAAGAATGACCCCATGGTCTGTGGGGTG	463	0.12383553143630495	No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	452	0.12089343457712706	No Hit
CAACAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAAC	451	0.12062597122629272	No Hit
CAACAAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	443	0.1184862644196179	No Hit
CAACAAGGGGAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAG	434	0.11607909426210875	No Hit
CAACAAGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG	427	0.11420685080626826	No Hit
CAACAAGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA	421	0.11260207070126216	No Hit
CAACAAGGGAAGGAAAAGGCAGAGAAGCTGAAGCAGTCCCTACCCCCGGG	418	0.11179968064875911	No Hit
CAACAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	417	0.11153221729792474	No Hit
CAACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	414	0.1107298272454217	No Hit
CAACAAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCT	401	0.10725280368457511	No Hit
CAACAAGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATC	391	0.1045781701762316	No Hit
CAACAAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGA	389	0.1040432434745629	No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCTGACTGC	388	0.10377578012372855	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.054027596868539085	0.0	0.0	0.0	0.0
7	0.05456252357020779	0.0	0.0	0.0	0.0
8	0.07729690839112771	0.0	0.0	0.0	0.0
9	0.10377578012372854	0.0	0.0	0.0	0.0
10	0.1690368377273104	0.0	0.0	0.0	0.0
11	0.23456535868172665	0.0	0.0	0.0	0.0
12	0.25221793983679386	0.0	0.0	0.0	0.0
13	0.26559110737851144	0.0	0.0	0.0	0.0
14	0.28458100528775043	0.0	0.0	0.0	0.0
15	0.30009387963614287	0.0	0.0	0.0	0.0
16	0.3177464607912101	0.0	0.0	0.0	0.0
17	0.33299187178876816	0.0	0.0	0.0	0.0
18	0.3471674293829888	0.0	0.0	0.0	0.0
19	0.3720415210105835	0.0	0.0	0.0	0.0
20	0.3827400550439576	0.0	0.0	0.0	0.0
21	0.3966481492873439	0.0	0.0	0.0	0.0
22	0.412695950337405	0.0	0.0	0.0	0.0
23	0.4335580917024845	0.0	0.0	0.0	0.0
24	0.4528154529625578	0.0	0.0	0.0	0.0
25	0.4688632540126189	0.0	0.0	0.0	0.0
26	0.4851785184135144	0.0	0.0	0.0	0.0
27	0.5022961728669129	0.0	0.0	0.0	0.0
28	0.5223559241794893	0.0	0.0	0.0	0.0
29	0.5432180655445688	0.0	0.0	0.0	0.0
30	0.5600682566471329	0.0	0.0	0.0	0.0
31	0.5774533744513658	0.0	0.0	0.0	0.0
32	0.5956408823081017	0.0	0.0	0.0	0.0
33	0.6130260001123347	0.0	0.0	0.0	0.0
34	0.6362953116349233	0.0	0.0	0.0	0.0
35	0.653680429439156	0.0	0.0	0.0	0.0
36	0.6705306205417203	0.0	0.0	0.0	0.0
37	0.6892530551001249	0.0	0.0	0.0	0.0
38	0.7058357828518548	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGACTA	65	0.0	44.000004	43
TAGCACG	65	0.0	44.000004	22
CTAGCAC	65	0.0	44.000004	21
TAATCAC	20	7.849659E-4	44.0	23
GCGAGAA	20	7.849659E-4	44.0	20
TCGCCCT	25	4.4370605E-5	44.0	42
TATTTCG	20	7.849659E-4	44.0	37
AACGTTT	20	7.849659E-4	44.0	21
ACTATTC	55	1.8189894E-12	44.0	42
AACCGTA	20	7.849659E-4	44.0	13
CGGAACT	40	8.287316E-9	44.0	33
GGTACTC	25	4.4370605E-5	44.0	9
TCGTTGA	20	7.849659E-4	44.0	14
TACCCGT	35	1.4425859E-7	44.0	35
ACACGCA	20	7.849659E-4	44.0	20
CATATTC	25	4.4370605E-5	44.0	41
GATAATG	20	7.849659E-4	44.0	10
CATATGA	20	7.849659E-4	44.0	25
GTCGATT	25	4.4370605E-5	44.0	44
GTCGATG	25	4.4370605E-5	44.0	42
>>END_MODULE