FastQCFastQC Report
Fri 17 Jun 2016
SRR1527902_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527902_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564184
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG56120.9947109453653418No Hit
CAACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT43140.7646441586432795No Hit
CAACAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG25530.45251194645718423No Hit
CAACAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT16210.28731761269373113No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT15390.27278334727677495No Hit
CAACAAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT15140.2683521687959956No Hit
CAACAAGGGGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGTC14220.25204543198672774No Hit
CAACAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG12860.22793982105128824No Hit
CAACAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT12740.22581285538051413No Hit
CAACAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA12610.2235086425705089No Hit
CAACAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG12240.2169504984189555No Hit
CAACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC11290.20011202019199412No Hit
CAACAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG10860.19249039320505368No Hit
CAACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10650.18876820328119903No Hit
CAACAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT10430.18486876621811324No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC10050.17813337492732867No Hit
CAACAAGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGA9880.17512017356039875No Hit
CAACAAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA8950.15863618961189965No Hit
CAACAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA8740.15491399968804503No Hit
CAACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8720.1545595054095827No Hit
CAACAAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC8580.15207804546034628No Hit
CAACAAGGGGGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC8260.14640613700494873No Hit
CAACAAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT8230.14587439558725523No Hit
CAACAAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT8230.14587439558725523No Hit
CAACAAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC7680.1361258029295407No Hit
CAACAAGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG7680.1361258029295407No Hit
CAACAAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG7640.13541681437261602No Hit
CAACAAGGGGGAGCTGGTGAGATGGCTCCGCAGTTAAGAGCACTGACTACTC7600.13470782581569135No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT7450.13204911872722375No Hit
CAACAAGGGGAGCATCAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGC7250.12850417594260027No Hit
CAACAAGGGATGTGTGTATGTATGTGTGTCTGTGTGTGTCTGTGTGTCCATG7090.12566822171490152No Hit
CAACAAGGGTAGCAAATCTACTCAAGAATGACCCCATGGTCTGTGGGGTGGG7080.12549097457567035No Hit
CAACAAGGGGACGGGGGTAGCGTGGCCGAGCGGTCTAAGGCGCTGGATTAAG7080.12549097457567035No Hit
CAACAAGGGAGATTTCTACCACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAA6910.12247777320874041No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG6840.1212370432341222No Hit
CAACAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC6820.12088254895565986No Hit
CAACAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA6810.12070530181642868No Hit
CAACAAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC6800.12052805467719752No Hit
CAACAAGGGGAGCACAGCTGGCCATCAGAGTCACCAATCCCAACGCCAGGCT6550.11609687619641819No Hit
CAACAAGGGGAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTG6530.11574238191795584No Hit
CAACAAGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGT6530.11574238191795584No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCTGACTGCTC6450.11432440480410647No Hit
CAACAAGGGAGTGAGCACACTGCTTCAAATAAGGTCATGTGTGAAAGAACAT6440.11414715766487529No Hit
CAACAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA6380.11308367482948825No Hit
CAACAAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA6280.11131120343717653No Hit
CAACAAGGGAAGGAAAAGGCAGAGAAGCTGAAGCAGTCCCTACCCCCGGGTT6230.11042496774102066No Hit
CAACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC6160.10918423776640246No Hit
CAACAAGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC6050.10723451923485955No Hit
CAACAAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC5850.10368957645023609No Hit
CAACAAGGGACCTACCCCCAGCTCCTCACAATAGGAATGTGGCTTCAGACCC5750.10191710505792437No Hit
CAACAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC5670.10049912794407498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACT351.01832484E-746.00000433
GCCATAT351.01832484E-746.00000418
GTTAAAC351.01832484E-746.00000412
CTCATAG351.01832484E-746.00000445
GAACGTT351.01832484E-746.00000439
AGATTCG351.01832484E-746.00000442
CCGAACG351.01832484E-746.00000437
TAGCTAT351.01832484E-746.00000418
ACGAAGC351.01832484E-746.00000418
ACGCGAA206.308708E-446.039
TATTTCG301.8597057E-646.037
AGCGTAC301.8597057E-646.023
AGACTCG206.308708E-446.034
TCGCATA206.308708E-446.045
ACCGGTT206.308708E-446.029
CGAACTA501.6370905E-1146.017
CATATTG206.308708E-446.032
AATCATC301.8597057E-646.044
CATATGA301.8597057E-646.025
GATCGTA405.6024874E-946.09
Produced by FastQC (version 0.11.5)