##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527902_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	564184
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.969495767338316	31.0	31.0	34.0	31.0	34.0
2	32.688945450420434	34.0	31.0	34.0	31.0	34.0
3	33.0530022120443	34.0	31.0	34.0	31.0	34.0
4	36.359499737674234	37.0	37.0	37.0	35.0	37.0
5	36.18190696652156	37.0	37.0	37.0	35.0	37.0
6	36.32257915857238	37.0	37.0	37.0	35.0	37.0
7	36.43654375168385	37.0	36.0	37.0	35.0	37.0
8	36.54611793315656	37.0	37.0	37.0	35.0	37.0
9	38.68708081051572	39.0	39.0	39.0	38.0	39.0
10	38.09289699814245	39.0	38.0	39.0	37.0	39.0
11	37.75319576592034	39.0	38.0	39.0	35.0	39.0
12	37.27687775619302	39.0	37.0	39.0	34.0	39.0
13	37.157969740368394	39.0	37.0	39.0	34.0	39.0
14	37.98594961927314	40.0	38.0	40.0	34.0	40.0
15	38.05082029976036	40.0	38.0	40.0	34.0	40.0
16	38.135186393091615	40.0	38.0	40.0	34.0	40.0
17	38.077416233002	40.0	38.0	40.0	34.0	40.0
18	38.010586971626275	40.0	38.0	40.0	34.0	40.0
19	38.04804460956	40.0	38.0	40.0	34.0	40.0
20	38.024201324390624	40.0	38.0	40.0	34.0	40.0
21	37.952768954809066	40.0	38.0	40.0	34.0	40.0
22	37.943412078329054	40.0	38.0	40.0	34.0	40.0
23	37.90481119634729	40.0	37.0	40.0	34.0	40.0
24	37.824399486692286	40.0	37.0	40.0	34.0	40.0
25	37.822479900174415	40.0	37.0	40.0	34.0	40.0
26	37.682612410135704	40.0	37.0	40.0	33.0	40.0
27	37.58737752222679	40.0	37.0	40.0	33.0	40.0
28	37.4563582093785	40.0	36.0	40.0	33.0	40.0
29	37.35933312536336	40.0	36.0	40.0	33.0	40.0
30	37.26310388100336	40.0	36.0	40.0	33.0	40.0
31	37.172075067708406	40.0	35.0	40.0	33.0	40.0
32	37.02814330076713	39.0	35.0	40.0	32.0	40.0
33	36.9017891326234	39.0	35.0	40.0	32.0	40.0
34	36.699837287126186	39.0	35.0	40.0	31.0	40.0
35	36.49297924081505	39.0	35.0	40.0	31.0	40.0
36	36.530996979708746	39.0	35.0	40.0	31.0	40.0
37	36.43666427973852	38.0	35.0	40.0	31.0	40.0
38	36.314851183301904	38.0	35.0	40.0	31.0	40.0
39	36.228430795626956	38.0	35.0	40.0	31.0	40.0
40	36.003199310863124	38.0	35.0	40.0	30.0	40.0
41	35.85303376203508	38.0	35.0	40.0	30.0	40.0
42	35.7518451427194	38.0	35.0	40.0	30.0	40.0
43	35.61073869517746	38.0	34.0	40.0	30.0	40.0
44	35.40743445400791	37.0	34.0	40.0	29.0	40.0
45	35.321460729123835	37.0	34.0	40.0	29.0	40.0
46	35.16032358237738	37.0	34.0	40.0	29.0	40.0
47	34.86717453880294	36.0	34.0	40.0	28.0	40.0
48	34.73551890872481	36.0	33.0	40.0	27.0	40.0
49	34.87784304411327	36.0	34.0	40.0	28.0	40.0
50	35.010115494235926	36.0	34.0	40.0	29.0	40.0
51	34.97647221473846	36.0	34.0	40.0	29.0	40.0
52	34.47204812614324	35.0	34.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	3.0
16	21.0
17	42.0
18	64.0
19	147.0
20	298.0
21	503.0
22	823.0
23	1211.0
24	1797.0
25	2491.0
26	3334.0
27	4494.0
28	5701.0
29	7230.0
30	9459.0
31	12175.0
32	15236.0
33	21716.0
34	38563.0
35	43228.0
36	61497.0
37	89393.0
38	165932.0
39	78825.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.45797824823107	0.4161762829147937	0.03544942784623457	0.09039604100789812
8	99.55900911759285	0.3513038299561845	0.06504970009784042	0.02463735235313302
9	99.15187243877884	0.5099400195680842	0.1329353544233796	0.2052521872296981
10	65.79413808261134	25.998433135289194	3.171837556541837	5.03559122555762
11	36.94486195992796	27.988564014576806	17.497483090622918	17.56909093487231
12	29.929242942018913	18.267621910582363	26.24267969314975	25.56045545424897
13	24.247940388242135	16.73638387476426	31.14179062149937	27.873885115494236
14	24.255562015229074	18.784297321441233	30.662868851296736	26.297271812032953
15	22.7273371807779	19.743204344681878	29.046197694369212	28.483260780171012
16	28.695780100109186	20.767870056577287	23.496058023623497	27.040291819690033
17	30.973228592090525	20.564390340739898	23.089453086227188	25.37292798094239
18	27.372984700026944	21.239524694071438	23.547991435418233	27.83949917048339
19	30.025842632899906	21.95329892375537	21.39390695234179	26.626951491002938
20	28.95385193482977	21.33506090211704	22.078258156913346	27.632829006139843
21	30.50440990882407	20.691653786707885	23.858173929072784	24.94576237539526
22	30.36757511733761	20.29515193624775	22.809757100520397	26.527515845894246
23	28.718467733930776	21.465514796591183	23.59301220878295	26.223005260695093
24	29.534690810090325	22.045112941877118	22.37177941948017	26.04841682855239
25	28.277831345802078	22.79362761085036	21.690795910554	27.237745132793556
26	27.234377437148165	22.43647462529955	21.92316691008607	28.405981027466215
27	26.554457411057385	21.558569544687547	23.190661202728187	28.696311841526878
28	29.1814372616026	21.524183599676704	20.312344908753172	28.982034229967528
29	29.63111325383208	22.35192773988628	23.047268267090168	24.96969073919147
30	28.824107028912554	20.180827531443644	22.61106305744225	28.38400238220155
31	28.270741460232834	22.94322419636147	21.87141074543057	26.91462359797513
32	31.928590672546548	22.823050635962737	21.680338329339364	23.568020362151355
33	32.96743615558045	21.416949080441842	21.07362137175106	24.54199339222665
34	28.11511847198786	25.544680458857393	23.391482211477108	22.948718857677637
35	27.896927243594288	24.62352707627299	25.87524637352353	21.604299306609192
36	29.492505990953305	23.386873785857095	22.041922493370958	25.07869772981864
37	27.78738851154942	26.03795924733775	21.11438821377423	25.060264027338597
38	31.880911192093357	25.391007189143966	21.052883456461014	21.67519816230166
39	28.996214001106026	24.49094621612807	20.511747940388243	26.001091842377665
40	27.637969173177545	24.16286176141117	21.95152645236306	26.247642613048228
41	27.793769408561747	23.83584078952966	23.41505608099485	24.955333720913746
42	27.0014746961984	22.790437162344197	21.78562313004268	28.422465011414715
43	26.942983140252114	20.819448974093557	25.021092409568507	27.216475476085815
44	26.014917119237698	20.10797895721963	25.43230577258483	28.444798150957844
45	27.670937141074543	19.939771422089247	25.238751896544393	27.150539540291817
46	28.057335904598503	22.709435219715555	22.94606015058917	26.287168725096777
47	27.06989209194164	24.293670150163777	22.771117224168	25.86532053372659
48	30.148674900387107	23.335294868340824	22.032528394991704	24.483501836280364
49	27.569906271712775	22.612481034556104	24.863342455652766	24.954270238078358
50	25.371155509550075	24.023368262836236	23.55844901663287	27.04702721098082
51	25.526955744934277	23.60683748564298	23.05347191696326	27.81273485245948
52	27.985550813209876	23.571742552075207	23.636260510755356	24.806446123959557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	7.0
4	14.0
5	33.0
6	52.0
7	32.0
8	12.0
9	110.5
10	209.0
11	1110.0
12	2011.0
13	2066.0
14	1847.5
15	1574.0
16	1336.0
17	1098.0
18	1247.5
19	1397.0
20	1389.0
21	1381.0
22	2140.5
23	2900.0
24	2725.5
25	2551.0
26	2638.0
27	2725.0
28	3117.5
29	3510.0
30	4419.0
31	5328.0
32	5304.5
33	5281.0
34	5738.5
35	6196.0
36	7628.5
37	9061.0
38	9466.0
39	11109.0
40	12347.0
41	13979.0
42	15611.0
43	15197.0
44	14783.0
45	17235.5
46	19688.0
47	21152.0
48	22616.0
49	22287.5
50	21959.0
51	25495.5
52	29032.0
53	30435.5
54	31839.0
55	37883.0
56	43927.0
57	49836.5
58	55746.0
59	52719.5
60	49693.0
61	48985.5
62	48278.0
63	44474.5
64	35108.0
65	29545.0
66	25343.5
67	21142.0
68	17836.0
69	14530.0
70	14129.0
71	13728.0
72	12803.5
73	11879.0
74	8952.0
75	6025.0
76	3922.5
77	1820.0
78	1638.5
79	1457.0
80	944.5
81	432.0
82	272.5
83	113.0
84	69.0
85	25.0
86	14.0
87	3.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	564184.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.636012364760433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.14598854750324	5.971456120698212
2	9.430864273546375	1.6289012095344784
3	4.289555240851343	1.1113395629794536
4	2.1406727828746175	0.739475064872453
5	1.1165158138866653	0.48211221870879006
6	0.7511852718428669	0.38923471775165547
7	0.4761611559222544	0.28784935411142465
8	0.3222297477577325	0.22262240687435306
9	0.2627096032674507	0.20418870439431108
>10	4.9073332922849575	12.02994058675893
>50	4.3121318473821395	26.962125831289086
>100	2.727664552675328	39.82530521957376
>500	0.0841491697966053	5.014498815989111
>1k	0.030786281632904373	4.136239241098648
>5k	0.0020524187755269585	0.9947109453653418
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5612	0.9947109453653418	No Hit
CAACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4314	0.7646441586432795	No Hit
CAACAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	2553	0.45251194645718423	No Hit
CAACAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1621	0.28731761269373113	No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	1539	0.27278334727677495	No Hit
CAACAAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	1514	0.2683521687959956	No Hit
CAACAAGGGGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGTC	1422	0.25204543198672774	No Hit
CAACAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1286	0.22793982105128824	No Hit
CAACAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1274	0.22581285538051413	No Hit
CAACAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1261	0.2235086425705089	No Hit
CAACAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1224	0.2169504984189555	No Hit
CAACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1129	0.20011202019199412	No Hit
CAACAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1086	0.19249039320505368	No Hit
CAACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1065	0.18876820328119903	No Hit
CAACAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	1043	0.18486876621811324	No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1005	0.17813337492732867	No Hit
CAACAAGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGA	988	0.17512017356039875	No Hit
CAACAAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	895	0.15863618961189965	No Hit
CAACAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	874	0.15491399968804503	No Hit
CAACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	872	0.1545595054095827	No Hit
CAACAAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	858	0.15207804546034628	No Hit
CAACAAGGGGGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	826	0.14640613700494873	No Hit
CAACAAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	823	0.14587439558725523	No Hit
CAACAAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT	823	0.14587439558725523	No Hit
CAACAAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	768	0.1361258029295407	No Hit
CAACAAGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG	768	0.1361258029295407	No Hit
CAACAAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	764	0.13541681437261602	No Hit
CAACAAGGGGGAGCTGGTGAGATGGCTCCGCAGTTAAGAGCACTGACTACTC	760	0.13470782581569135	No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT	745	0.13204911872722375	No Hit
CAACAAGGGGAGCATCAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGC	725	0.12850417594260027	No Hit
CAACAAGGGATGTGTGTATGTATGTGTGTCTGTGTGTGTCTGTGTGTCCATG	709	0.12566822171490152	No Hit
CAACAAGGGTAGCAAATCTACTCAAGAATGACCCCATGGTCTGTGGGGTGGG	708	0.12549097457567035	No Hit
CAACAAGGGGACGGGGGTAGCGTGGCCGAGCGGTCTAAGGCGCTGGATTAAG	708	0.12549097457567035	No Hit
CAACAAGGGAGATTTCTACCACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	691	0.12247777320874041	No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	684	0.1212370432341222	No Hit
CAACAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC	682	0.12088254895565986	No Hit
CAACAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA	681	0.12070530181642868	No Hit
CAACAAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	680	0.12052805467719752	No Hit
CAACAAGGGGAGCACAGCTGGCCATCAGAGTCACCAATCCCAACGCCAGGCT	655	0.11609687619641819	No Hit
CAACAAGGGGAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTG	653	0.11574238191795584	No Hit
CAACAAGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGT	653	0.11574238191795584	No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCTGACTGCTC	645	0.11432440480410647	No Hit
CAACAAGGGAGTGAGCACACTGCTTCAAATAAGGTCATGTGTGAAAGAACAT	644	0.11414715766487529	No Hit
CAACAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	638	0.11308367482948825	No Hit
CAACAAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA	628	0.11131120343717653	No Hit
CAACAAGGGAAGGAAAAGGCAGAGAAGCTGAAGCAGTCCCTACCCCCGGGTT	623	0.11042496774102066	No Hit
CAACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	616	0.10918423776640246	No Hit
CAACAAGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC	605	0.10723451923485955	No Hit
CAACAAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC	585	0.10368957645023609	No Hit
CAACAAGGGACCTACCCCCAGCTCCTCACAATAGGAATGTGGCTTCAGACCC	575	0.10191710505792437	No Hit
CAACAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	567	0.10049912794407498	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.042362066276250304	0.0	0.0	0.0	0.0
7	0.043248301972406165	0.0	0.0	0.0	0.0
8	0.061859251591679314	0.0	0.0	0.0	0.0
9	0.08295166116018887	0.0	0.0	0.0	0.0
10	0.1368347914864654	0.0	0.0	0.0	0.0
11	0.19178140464812898	0.0	0.0	0.0	0.0
12	0.20702465862200983	0.0	0.0	0.0	0.0
13	0.21872296981126724	0.0	0.0	0.0	0.0
14	0.23325723522822342	0.0	0.0	0.0	0.0
15	0.2483232420628731	0.0	0.0	0.0	0.0
16	0.2665796974036839	0.0	0.0	0.0	0.0
17	0.2814684570991024	0.0	0.0	0.0	0.0
18	0.2915715440352793	0.0	0.0	0.0	0.0
19	0.31957659203380456	0.0	0.0	0.0	0.0
20	0.33411085745076075	0.0	0.0	0.0	0.0
21	0.3489996171461793	0.0	0.0	0.0	0.0
22	0.36282489400621076	0.0	0.0	0.0	0.0
23	0.3839173035747203	0.0	0.0	0.0	0.0
24	0.40642769025707925	0.0	0.0	0.0	0.0
25	0.42291167420557835	0.0	0.0	0.0	0.0
26	0.43620520964791626	0.0	0.0	0.0	0.0
27	0.4542844178494959	0.0	0.0	0.0	0.0
28	0.4732498617472314	0.0	0.0	0.0	0.0
29	0.4876068800249564	0.0	0.0	0.0	0.0
30	0.5049770996696114	0.0	0.0	0.0	0.0
31	0.5198658593650298	0.0	0.0	0.0	0.0
32	0.5421989989081576	0.0	0.0	0.0	0.0
33	0.5567332643251138	0.0	0.0	0.0	0.0
34	0.580484380982091	0.0	0.0	0.0	0.0
35	0.5983863420444394	0.0	0.0	0.0	0.0
36	0.6168200445244814	0.0	0.0	0.0	0.0
37	0.6325950399160557	0.0	0.0	0.0	0.0
38	0.6499652595607107	0.0	0.0	0.0	0.0
39	0.6668037377876721	0.0	0.0	0.0	0.0
40	0.68311047459694	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAACT	35	1.01832484E-7	46.000004	33
GCCATAT	35	1.01832484E-7	46.000004	18
GTTAAAC	35	1.01832484E-7	46.000004	12
CTCATAG	35	1.01832484E-7	46.000004	45
GAACGTT	35	1.01832484E-7	46.000004	39
AGATTCG	35	1.01832484E-7	46.000004	42
CCGAACG	35	1.01832484E-7	46.000004	37
TAGCTAT	35	1.01832484E-7	46.000004	18
ACGAAGC	35	1.01832484E-7	46.000004	18
ACGCGAA	20	6.308708E-4	46.0	39
TATTTCG	30	1.8597057E-6	46.0	37
AGCGTAC	30	1.8597057E-6	46.0	23
AGACTCG	20	6.308708E-4	46.0	34
TCGCATA	20	6.308708E-4	46.0	45
ACCGGTT	20	6.308708E-4	46.0	29
CGAACTA	50	1.6370905E-11	46.0	17
CATATTG	20	6.308708E-4	46.0	32
AATCATC	30	1.8597057E-6	46.0	44
CATATGA	30	1.8597057E-6	46.0	25
GATCGTA	40	5.6024874E-9	46.0	9
>>END_MODULE