FastQCFastQC Report
Fri 17 Jun 2016
SRR1527901_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527901_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569012
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG53840.946201486084652No Hit
CAACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT42580.7483146225387163No Hit
CAACAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG24580.4319768300141298No Hit
CAACAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT16210.28487975649019703No Hit
CAACAAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT14980.26326334066768364No Hit
CAACAAGGGGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGTC13710.24094395197289337No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT13590.2388350333560628No Hit
CAACAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG12170.21387949639023432No Hit
CAACAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA11810.2075527405397426No Hit
CAACAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT11450.20122598468925085No Hit
CAACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC11070.19454774240262068No Hit
CAACAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG10960.19261456700385932No Hit
CAACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10210.17943382564866822No Hit
CAACAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT10000.17574321806921472No Hit
CAACAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG9840.17293132658010726No Hit
CAACAAGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGA9240.1623867334959544No Hit
CAACAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA9200.16168376062367754No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC9050.1590476123526393No Hit
CAACAAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA8680.15254511328407835No Hit
CAACAAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC8490.1492059921407633No Hit
CAACAAGGGGGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC8260.14516389812517133No Hit
CAACAAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT8180.14375795238061764No Hit
CAACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8080.14200052019992548No Hit
CAACAAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT7420.1304014678073573No Hit
CAACAAGGGGACGGGGGTAGCGTGGCCGAGCGGTCTAAGGCGCTGGATTAAG7250.12741383310018067No Hit
CAACAAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC7150.12565640091948851No Hit
CAACAAGGGGGAGCTGGTGAGATGGCTCCGCAGTTAAGAGCACTGACTACTC7000.1230202526484503No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT6960.12231727977617343No Hit
CAACAAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG6870.1207355908135505No Hit
CAACAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA6850.12038410437741208No Hit
CAACAAGGGTAGCAAATCTACTCAAGAATGACCCCATGGTCTGTGGGGTGGG6790.11932964506899679No Hit
CAACAAGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG6770.11897815863285835No Hit
CAACAAGGGATGTGTGTATGTATGTGTGTCTGTGTGTGTCTGTGTGTCCATG6760.11880241541478914No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG6710.11792369932444306No Hit
CAACAAGGGAGATTTCTACCACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAA6630.11651775357988935No Hit
CAACAAGGGGAGCACAGCTGGCCATCAGAGTCACCAATCCCAACGCCAGGCT6520.114584578181128No Hit
CAACAAGGGGAGCATCAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGC6440.11317863243657426No Hit
CAACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC6410.11265140278236663No Hit
CAACAAGGGGAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTG6320.11106971381974369No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCTGACTGCTC6240.10966376807518999No Hit
CAACAAGGGAGTGAGCACACTGCTTCAAATAAGGTCATGTGTGAAAGAACAT6220.10931228163905155No Hit
CAACAAGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGT6210.10913653842098234No Hit
CAACAAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC6200.10896079520291312No Hit
CAACAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA6070.10667613336801332No Hit
CAACAAGGGAAGGAAAAGGCAGAGAAGCTGAAGCAGTCCCTACCCCCGGGTT6030.10597316049573648No Hit
CAACAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC5960.10474295796925195No Hit
CAACAAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA5920.10403998509697511No Hit
CAACAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC5770.10140383682593689No Hit
CAACAAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA5730.10070086395366003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACT351.0183612E-746.00000433
CTCAACG351.0183612E-746.00000422
TTTACCG351.0183612E-746.00000415
TACGGTT351.0183612E-746.00000439
TGTGCGA351.0183612E-746.00000419
TCGGAAT700.046.00000433
TACGAGT351.0183612E-746.00000431
CGTAGCG750.046.00000412
CCATAAG351.0183612E-746.00000422
ACGCGAA253.4149656E-546.039
TCACGCA206.3087547E-446.025
CTATGTC253.4149656E-546.015
CAGGTAA405.6024874E-946.031
AACCGTT301.8597348E-646.019
TAATAGT253.4149656E-546.019
ACCGGTA206.3087547E-446.038
CATATTG301.8597348E-646.032
GATCGTA206.3087547E-446.09
GGTCGTA206.3087547E-446.017
ATAGGTA301.8597348E-646.023
Produced by FastQC (version 0.11.5)