##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527901_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	569012
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.903850885394334	31.0	31.0	34.0	31.0	34.0
2	32.658915804939085	34.0	31.0	34.0	31.0	34.0
3	33.03718023521472	34.0	31.0	34.0	31.0	34.0
4	36.345201858660275	37.0	37.0	37.0	35.0	37.0
5	36.19464264374038	37.0	37.0	37.0	35.0	37.0
6	36.245131912859485	37.0	37.0	37.0	35.0	37.0
7	36.38694614524825	37.0	36.0	37.0	35.0	37.0
8	36.50979592697518	37.0	37.0	37.0	35.0	37.0
9	38.64197943101376	39.0	39.0	39.0	38.0	39.0
10	37.99907910553732	39.0	38.0	39.0	37.0	39.0
11	37.72712350530393	39.0	38.0	39.0	35.0	39.0
12	37.223707057144665	39.0	37.0	39.0	34.0	39.0
13	36.95237886019979	39.0	37.0	39.0	33.0	39.0
14	37.91890329202196	40.0	38.0	40.0	33.0	40.0
15	37.99939368589766	40.0	38.0	40.0	34.0	40.0
16	38.1135582377876	40.0	38.0	40.0	34.0	40.0
17	38.08340773129565	40.0	38.0	40.0	34.0	40.0
18	38.043151989764716	40.0	38.0	40.0	34.0	40.0
19	38.0321487068814	40.0	38.0	40.0	34.0	40.0
20	38.01492235664626	40.0	38.0	40.0	34.0	40.0
21	37.99704927136862	40.0	38.0	40.0	34.0	40.0
22	37.87622580894603	40.0	38.0	40.0	34.0	40.0
23	37.8686284296289	40.0	37.0	40.0	34.0	40.0
24	37.83483476622637	40.0	37.0	40.0	34.0	40.0
25	37.803359155870176	40.0	37.0	40.0	34.0	40.0
26	37.61132981378248	40.0	37.0	40.0	33.0	40.0
27	37.51449178576199	40.0	36.0	40.0	33.0	40.0
28	37.41575748841852	40.0	36.0	40.0	33.0	40.0
29	37.31982629540326	40.0	36.0	40.0	33.0	40.0
30	37.212466520916955	40.0	36.0	40.0	33.0	40.0
31	37.14905836783758	40.0	35.0	40.0	33.0	40.0
32	37.000720547194085	39.0	35.0	40.0	32.0	40.0
33	36.68629132601773	39.0	35.0	40.0	31.0	40.0
34	36.60372891960099	39.0	35.0	40.0	31.0	40.0
35	36.47761910117889	39.0	35.0	40.0	31.0	40.0
36	36.36355472292324	39.0	35.0	40.0	31.0	40.0
37	36.34261667592248	38.0	35.0	40.0	31.0	40.0
38	35.94375338305695	38.0	35.0	40.0	30.0	40.0
39	36.03362494991318	38.0	35.0	40.0	30.0	40.0
40	35.88119934201739	38.0	35.0	40.0	30.0	40.0
41	35.521614658390334	38.0	34.0	40.0	29.0	40.0
42	35.567197176860944	38.0	34.0	40.0	29.0	40.0
43	35.538452616113545	38.0	34.0	40.0	29.0	40.0
44	35.34436883580663	37.0	34.0	40.0	29.0	40.0
45	35.25198238349982	37.0	34.0	40.0	29.0	40.0
46	34.9211756518316	37.0	34.0	40.0	28.0	40.0
47	34.56105670882161	36.0	33.0	40.0	26.0	40.0
48	34.58522667360267	36.0	33.0	40.0	27.0	40.0
49	34.36370058979424	35.0	33.0	39.0	27.0	40.0
50	34.29075133740589	35.0	33.0	39.0	27.0	40.0
51	34.56243277821909	35.0	33.0	39.0	28.0	40.0
52	34.28828917492074	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	2.0
15	3.0
16	17.0
17	50.0
18	98.0
19	173.0
20	327.0
21	487.0
22	921.0
23	1255.0
24	1852.0
25	2681.0
26	3542.0
27	4704.0
28	6047.0
29	7670.0
30	9943.0
31	12696.0
32	16445.0
33	23251.0
34	39469.0
35	45129.0
36	64976.0
37	94923.0
38	164967.0
39	67382.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.46187426627206	0.4024519693785017	0.04463877738958054	0.09103498695985321
8	99.5467582405995	0.3542983276275368	0.0692428279192706	0.029700603853697286
9	99.14377904156679	0.510006818836861	0.14973322179497095	0.19648091780138205
10	65.82181043633526	25.982053102570774	3.157051169395373	5.039085291698593
11	36.994650376441975	28.080602869535266	17.396293927017357	17.528452827005406
12	29.885837205542238	18.258314411646854	26.189605843110513	25.666242539700395
13	24.54781269990791	16.757115842899623	30.93678164959614	27.758289807596327
14	24.295269695542448	18.864277027549505	30.68441438844875	26.156038888459292
15	22.67017215805642	19.755119399942355	29.040160840193174	28.534547601808047
16	28.569520502203822	20.76581864705841	23.55257885598195	27.112081994755822
17	30.87263537500088	20.51257266982067	23.20267410880614	25.412117846372308
18	27.366909660956185	21.08356238532755	23.515848523405484	28.03367943031078
19	29.978629624682785	22.018340562237704	21.34085045658088	26.662179356498633
20	28.92996984246378	21.317476608577675	22.174400539883166	27.57815300907538
21	30.575629336463905	20.552114893886245	23.850463610609264	25.021792159040583
22	30.60146358952008	20.201331430620094	22.727640190365054	26.469564789494775
23	28.648429207116898	21.56685623501789	23.49388062114683	26.290833936718382
24	29.557548874188942	22.145578652119816	22.399176115793693	25.897696357897548
25	28.20063548747654	22.823420244212773	21.697609189261385	27.2783350790493
26	27.185894146344893	22.385468144784294	22.12589541169606	28.302742297174753
27	26.623515848523404	21.62080940296514	23.082290004428728	28.673384744082725
28	29.234884325813866	21.52889569991494	20.23085629125572	29.005363683015474
29	29.67547257351339	22.33046051752863	23.082641490864866	24.911425418093117
30	28.902026670790775	20.081123069460748	22.62377594848615	28.39307431126233
31	28.216803863538907	22.873507061362503	21.906743618763752	27.002945456334842
32	31.864705841001594	22.942046916409495	21.617646025039893	23.575601217549014
33	32.89860319290278	21.517999620394647	21.136988323620592	24.446408863081974
34	28.192199813009218	25.526702424553438	23.411632795090437	22.86946496734691
35	27.8806070873725	24.64007788939425	25.820545085165165	21.65876993806809
36	29.765804587600964	23.20724343247594	21.941365032723386	25.085586947199705
37	27.912592353061093	25.847258054311684	21.226441621617823	25.013707971009396
38	31.68861113649624	25.403506428686917	21.154211158991373	21.753671275825464
39	29.047190568915948	24.433052378508712	20.464594771287775	26.055162281287565
40	27.655128538589697	23.978228930145587	22.04856839574561	26.318074135519108
41	28.019444229647178	23.761713285484312	23.409875362909744	24.808967121958762
42	26.978165662587077	22.75329870020316	21.8317012646482	28.436834372561563
43	26.917007022698996	20.784798914609883	25.090683500523713	27.207510562167407
44	26.005778437010115	20.25370290960472	25.42811047921661	28.312408174168556
45	27.536853352829116	19.86794654594279	25.389095484805242	27.206104616422856
46	28.269175342523532	22.580015887186914	23.043099266799295	26.107709503490263
47	27.192572388631525	24.206870856853634	22.77790275073285	25.82265400378199
48	29.977750908592437	23.322530983529347	22.121501831244334	24.578216276633885
49	27.654777052153555	22.519384476953032	24.799476988183024	25.026361482710378
50	25.410184670973546	23.927263396905513	23.441333398944135	27.221218533176806
51	25.545331205668774	23.604247362094295	22.940113741010734	27.910307691226194
52	28.145803603438946	23.51356386157058	23.454338397081255	24.886294137909218
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	5.0
4	10.0
5	22.5
6	35.0
7	27.5
8	20.0
9	105.5
10	191.0
11	1053.0
12	1915.0
13	1974.5
14	1859.5
15	1685.0
16	1398.5
17	1112.0
18	1274.5
19	1437.0
20	1398.0
21	1359.0
22	2088.0
23	2817.0
24	2720.5
25	2624.0
26	2622.5
27	2621.0
28	3072.5
29	3524.0
30	4347.0
31	5170.0
32	5245.5
33	5321.0
34	5819.0
35	6317.0
36	7742.0
37	9167.0
38	9589.0
39	11096.5
40	12182.0
41	14001.5
42	15821.0
43	15431.0
44	15041.0
45	17434.0
46	19827.0
47	21361.0
48	22895.0
49	22635.5
50	22376.0
51	25860.5
52	29345.0
53	30901.5
54	32458.0
55	38205.5
56	43953.0
57	50024.0
58	56095.0
59	53265.0
60	50435.0
61	49574.0
62	48713.0
63	44925.0
64	35442.5
65	29748.0
66	25564.0
67	21380.0
68	18053.5
69	14727.0
70	14246.5
71	13766.0
72	12805.0
73	11844.0
74	8862.5
75	5881.0
76	3921.0
77	1961.0
78	1691.0
79	1421.0
80	946.5
81	472.0
82	304.5
83	137.0
84	75.5
85	14.0
86	10.0
87	6.0
88	5.5
89	2.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	569012.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.4608971339796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.42457407198465	9.27396961751246
2	9.770957914927225	2.435098029567039
3	3.6274399187633986	1.3560346706220607
4	1.6303734627101434	0.8126366403520487
5	0.8955771183572153	0.5579847173697567
6	0.6050434390161344	0.45236304331015864
7	0.35682048967618185	0.3112412392005793
8	0.2623265260069954	0.26150590848699146
9	0.18052578133814734	0.20245618721573536
>10	3.523073451427282	12.52592212466521
>50	2.9025160780774004	26.006305666664325
>100	1.741791718379781	36.28306608647972
>500	0.06064537966828388	5.177746690755203
>1k	0.016924292000451313	3.397467891714059
>5k	0.001410357666704276	0.946201486084652
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5384	0.946201486084652	No Hit
CAACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4258	0.7483146225387163	No Hit
CAACAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	2458	0.4319768300141298	No Hit
CAACAAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1621	0.28487975649019703	No Hit
CAACAAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	1498	0.26326334066768364	No Hit
CAACAAGGGGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGTC	1371	0.24094395197289337	No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	1359	0.2388350333560628	No Hit
CAACAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1217	0.21387949639023432	No Hit
CAACAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1181	0.2075527405397426	No Hit
CAACAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1145	0.20122598468925085	No Hit
CAACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1107	0.19454774240262068	No Hit
CAACAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1096	0.19261456700385932	No Hit
CAACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1021	0.17943382564866822	No Hit
CAACAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	1000	0.17574321806921472	No Hit
CAACAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	984	0.17293132658010726	No Hit
CAACAAGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGA	924	0.1623867334959544	No Hit
CAACAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	920	0.16168376062367754	No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	905	0.1590476123526393	No Hit
CAACAAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	868	0.15254511328407835	No Hit
CAACAAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	849	0.1492059921407633	No Hit
CAACAAGGGGGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	826	0.14516389812517133	No Hit
CAACAAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	818	0.14375795238061764	No Hit
CAACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	808	0.14200052019992548	No Hit
CAACAAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT	742	0.1304014678073573	No Hit
CAACAAGGGGACGGGGGTAGCGTGGCCGAGCGGTCTAAGGCGCTGGATTAAG	725	0.12741383310018067	No Hit
CAACAAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	715	0.12565640091948851	No Hit
CAACAAGGGGGAGCTGGTGAGATGGCTCCGCAGTTAAGAGCACTGACTACTC	700	0.1230202526484503	No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT	696	0.12231727977617343	No Hit
CAACAAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	687	0.1207355908135505	No Hit
CAACAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA	685	0.12038410437741208	No Hit
CAACAAGGGTAGCAAATCTACTCAAGAATGACCCCATGGTCTGTGGGGTGGG	679	0.11932964506899679	No Hit
CAACAAGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG	677	0.11897815863285835	No Hit
CAACAAGGGATGTGTGTATGTATGTGTGTCTGTGTGTGTCTGTGTGTCCATG	676	0.11880241541478914	No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	671	0.11792369932444306	No Hit
CAACAAGGGAGATTTCTACCACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	663	0.11651775357988935	No Hit
CAACAAGGGGAGCACAGCTGGCCATCAGAGTCACCAATCCCAACGCCAGGCT	652	0.114584578181128	No Hit
CAACAAGGGGAGCATCAGTTCCTGGGTCCTGTCCGAAGGCACGCTCAGGAGC	644	0.11317863243657426	No Hit
CAACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	641	0.11265140278236663	No Hit
CAACAAGGGGAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTG	632	0.11106971381974369	No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCTGACTGCTC	624	0.10966376807518999	No Hit
CAACAAGGGAGTGAGCACACTGCTTCAAATAAGGTCATGTGTGAAAGAACAT	622	0.10931228163905155	No Hit
CAACAAGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGT	621	0.10913653842098234	No Hit
CAACAAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	620	0.10896079520291312	No Hit
CAACAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	607	0.10667613336801332	No Hit
CAACAAGGGAAGGAAAAGGCAGAGAAGCTGAAGCAGTCCCTACCCCCGGGTT	603	0.10597316049573648	No Hit
CAACAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC	596	0.10474295796925195	No Hit
CAACAAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA	592	0.10403998509697511	No Hit
CAACAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	577	0.10140383682593689	No Hit
CAACAAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	573	0.10070086395366003	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0418268859004731	0.0	0.0	0.0	0.0
7	0.04217837233661153	0.0	0.0	0.0	0.0
8	0.05869823483511771	0.0	0.0	0.0	0.0
9	0.08013890743956191	0.0	0.0	0.0	0.0
10	0.1337405889506724	0.0	0.0	0.0	0.0
11	0.1864635543714368	0.0	0.0	0.0	0.0
12	0.20333490330608142	0.0	0.0	0.0	0.0
13	0.2161641582251341	0.0	0.0	0.0	0.0
14	0.23092658854294812	0.0	0.0	0.0	0.0
15	0.2449860459884853	0.0	0.0	0.0	0.0
16	0.25851827377981484	0.0	0.0	0.0	0.0
17	0.27169901513500594	0.0	0.0	0.0	0.0
18	0.28400104039985097	0.0	0.0	0.0	0.0
19	0.3117684688547869	0.0	0.0	0.0	0.0
20	0.32319177802928584	0.0	0.0	0.0	0.0
21	0.33848143800130753	0.0	0.0	0.0	0.0
22	0.3525408954468447	0.0	0.0	0.0	0.0
23	0.37239987908866595	0.0	0.0	0.0	0.0
24	0.39173163307627956	0.0	0.0	0.0	0.0
25	0.4040336583411246	0.0	0.0	0.0	0.0
26	0.4228381826745306	0.0	0.0	0.0	0.0
27	0.4386550723007599	0.0	0.0	0.0	0.0
28	0.45412047549085083	0.0	0.0	0.0	0.0
29	0.47134331086163384	0.0	0.0	0.0	0.0
30	0.4864572276155863	0.0	0.0	0.0	0.0
31	0.499286482534639	0.0	0.0	0.0	0.0
32	0.5175637772138373	0.0	0.0	0.0	0.0
33	0.5298658024786823	0.0	0.0	0.0	0.0
34	0.5530639072638187	0.0	0.0	0.0	0.0
35	0.5702867426346018	0.0	0.0	0.0	0.0
36	0.5857521458246926	0.0	0.0	0.0	0.0
37	0.6031507244135449	0.0	0.0	0.0	0.0
38	0.6179131547313589	0.0	0.0	0.0	0.0
39	0.635135990102142	0.0	0.0	0.0	0.0
40	0.6488439611115407	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAACT	35	1.0183612E-7	46.000004	33
CTCAACG	35	1.0183612E-7	46.000004	22
TTTACCG	35	1.0183612E-7	46.000004	15
TACGGTT	35	1.0183612E-7	46.000004	39
TGTGCGA	35	1.0183612E-7	46.000004	19
TCGGAAT	70	0.0	46.000004	33
TACGAGT	35	1.0183612E-7	46.000004	31
CGTAGCG	75	0.0	46.000004	12
CCATAAG	35	1.0183612E-7	46.000004	22
ACGCGAA	25	3.4149656E-5	46.0	39
TCACGCA	20	6.3087547E-4	46.0	25
CTATGTC	25	3.4149656E-5	46.0	15
CAGGTAA	40	5.6024874E-9	46.0	31
AACCGTT	30	1.8597348E-6	46.0	19
TAATAGT	25	3.4149656E-5	46.0	19
ACCGGTA	20	6.3087547E-4	46.0	38
CATATTG	30	1.8597348E-6	46.0	32
GATCGTA	20	6.3087547E-4	46.0	9
GGTCGTA	20	6.3087547E-4	46.0	17
ATAGGTA	30	1.8597348E-6	46.0	23
>>END_MODULE