FastQCFastQC Report
Fri 17 Jun 2016
SRR1527897_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527897_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131703
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC72625.513921474833527No Hit
ACGTTGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA25521.9376931429048692No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12850.9756801287745913No Hit
ACGTTGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT11390.864824643326272No Hit
ACGTTGGGGGGGCTGGAGAGATGGCTCAGAGGTCAGAGCACTGTCTGTTC7120.5406103125972833No Hit
ACGTTGGGGACCCACCTAGCCAGAGGTCCCATGTCCTTGGCAGTGAACTC5370.40773558688868133No Hit
ACGTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC4800.36445639051502243No Hit
ACGTTGGGGTGGGCTGGCGAGATGGCTCAACGGGTAAGAGCACTGACTGC4420.33560359293258313No Hit
ACGTTGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCAGTGAGTGC4380.33256645634495796No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG4210.3196586258475509No Hit
ACGTTGGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGA4210.3196586258475509No Hit
ACGTTGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCT4070.3090286477908628No Hit
ACGTTGGGGCGGCATTAGCCGTTCAATCCAGCGCGTACCGATTTTCATCA3990.30295437461561237No Hit
ACGTTGGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGC3950.29991723802798725No Hit
ACGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3940.2991579538810809No Hit
ACGTTGGGGGGGGCGGTGGTAGCGCACGCCTTTAATCCTAGCACTTGGGA3800.28852797582439277No Hit
ACGTTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA3500.26574945141720385Illumina Single End Adapter 2 (100% over 34bp)
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACGTTGGGGA3410.25891589409504717No Hit
ACGTTGGGGGTTGGCCAGGGAGATCTCCCCGCAAGGGTTGGTCCCTTCCA3400.2581566099481409No Hit
ACGTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAG3340.2536009050667031No Hit
ACGTTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC3230.24524877945073387No Hit
ACGTTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA3130.23765593798167087Illumina Single End Adapter 2 (100% over 34bp)
ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3110.2361373696878583No Hit
ACGTTGGGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAA3080.2338595172471394No Hit
ACGTTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3040.23082238065951421Illumina Single End Adapter 2 (97% over 34bp)
ACGTTGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA2710.20576600381160642Illumina Single End Adapter 2 (97% over 34bp)
ACGTTGGGGGGGAGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTG2690.2042474355177938No Hit
ACGTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA2560.19437674160801197No Hit
ACGTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC2520.19133960502038677No Hit
ACGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT2510.19058032087348048No Hit
ACGTTGGGGAATGAAGCATTTAGCTACAGAAATCTAGAGGTCCAAGTCAT2490.18906175257966787No Hit
ACGTTGGGGGAGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAA2440.1852653318451364No Hit
ACGTTGGGGACAGGGTCTAACCCCACTGTTTAAGAGGCTGTGCTTTATAT2400.18222819525751122No Hit
ACGTTGGCTGACGACCGATTAGAGGCGTTTTATGATAATCCCAATGCTTT2390.18146891111060492No Hit
ACGTTGGGGCTTCCACCAAGATATCTTGTAAGCTTTGATGTTTTGTATAA2250.17083893305391676No Hit
ACGTTGGGGCCTTCTCCCCGGCGGTTAGTGCTGAGAGTGCGGAGTGTGTG2240.1700796489070105No Hit
ACGTTGGGGGTGCTACGTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGC2230.16932036476010418No Hit
ACGTTGGAGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGC2230.16932036476010418No Hit
ACGTTGGGGAGGGTTTCAGAAGAAAGAGAGAGATCAGATAGGCCCAGGAA2150.1632460915848538No Hit
ACGTTGGGGATGTTCAAAAATGAGGACTAAGAACCAAGTTTTGGCACCTT2130.1617275232910412No Hit
ACGTTGGGGGACTGGGCAGTGGGCCATGCTGTGTGGGCAGGATCAGGGAG2130.1617275232910412No Hit
ACGTTGGGGTGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGG2120.1609682391441349No Hit
ACGTTGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTC2060.15641253426269713No Hit
ACGTTGGGGAGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGG2040.15489396596888452No Hit
ACGTTGGGGGTGCTACGTTGGGGATGGATGTTTGTGCTCTCTCTGCTCCT2040.15489396596888452No Hit
ACGTTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2020.15337539767507194Illumina Single End Adapter 2 (97% over 34bp)
ACGTTGGGATACTTTTGTTTTTTTATGTATGCATTAGAATGCAGCAATAT2010.15261611352816565No Hit
ACGTTGGGGGTGCTACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAA1980.15033826108744675No Hit
ACGTTGGGGAAAAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCTTCT1950.14806040864672787Illumina Single End Adapter 2 (100% over 29bp)
ACGTTGGGGTTGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG1870.14198613547147748No Hit
ACGTTGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1850.1404675671776649Illumina Single End Adapter 2 (97% over 34bp)
ACGTTGGGGTGCCGGTAGTGATGGCGCACGCCGGTAGGATTTGCTGAAGG1850.1404675671776649No Hit
ACGTTGGGTGCTGGAGACATGGCTCAGTGGTTAAGAGCACCGACTGCTCT1840.1397082830307586No Hit
ACGTTGGGGAAGCATAGCAACAGTGAACATAAAGATTCTGAAAAGAAACA1780.13515257814932083No Hit
ACGTTGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCTT1770.13439329400241454Illumina Single End Adapter 2 (100% over 27bp)
ACGTTGGGGTATTCTATTGAAAAGGAATCCTAATAATTCATTGGGTAGGA1770.13439329400241454No Hit
ACGTTGGGGGAGTTCCAGACTAGTAAGTGACCTTGTCCCAAATAAAAGGT1760.13363400985550822No Hit
ACGTTGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA1750.13287472570860193No Hit
ACGTTGAGGATTTGTGGCTCTTGCAAAGGACACAGTTTCAATTCCAAGTA1750.13287472570860193No Hit
ACGTTGGGGCCTCCTGCTGCAGTCCTGAACTCTCTCCCTCTGATGTGTCC1750.13287472570860193No Hit
ACGTTGGGTATTGGAGAGCAACAGGGAAGGGGCCTCTTCCATCAGCAGAG1730.13135615741478932No Hit
ACGTTGGGGAAAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCTTCTG1730.13135615741478932Illumina Single End Adapter 2 (100% over 30bp)
ACGTTGGGGTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT1710.12983758912097676No Hit
ACGTTGGGGGCAGAGTGCAGTGCTACGTTGGGGGCTGGTGAGATGGCTCA1700.12907830497407044No Hit
ACGTTGGGGTTCATGACCATGAAACATCCATTTTAAAGCACTGATTTTTG1650.12528188423953898No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCT1640.12452260009263266Illumina Single End Adapter 2 (100% over 26bp)
ACGTTGCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGT1630.12376331594572637No Hit
ACGTTGGGGAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT1620.12300403179882008Illumina Single End Adapter 2 (100% over 32bp)
ACGTTGGGGGTGGCCATGAAAACTCCCCTGCCAGGTAAGTACAACACAAA1620.12300403179882008No Hit
ACGTTGGGGATGGTATGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACC1610.12224474765191377No Hit
ACGTTGGGTGTGTACCAGCACAAATACTGCTTCCTTTGGTCCCCCAGAGC1590.12072617935810118No Hit
ACGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1590.12072617935810118No Hit
ACGTTGGGGAAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGC1580.11996689521119488Illumina Single End Adapter 2 (100% over 31bp)
ACGTTGGGGAACCTGTCAGCTTTTAGAGACAGTAGTAGCTCATCACAGGA1580.11996689521119488No Hit
ACGTTGGGGGCACTCTCTCCAGTCTCTGTTCTCTCTCCTCCGCGCCCCCC1580.11996689521119488No Hit
ACGTTGGGGACCAGGTCAACGTGAGGGGCTTACATTCTGGGTTTGATAAG1570.1192076110642886No Hit
ACGTTGGGGACTCGGGTTGTACCAGTGACCGCTGTATGTTACAGCCTTCT1560.11844832691738229No Hit
ACGTTGGGGATTAGCCTAGTTTCGTGAGGCAGAACGACCAGGATAAGATA1510.11465190618285082No Hit
ACGTTGGGGAAAAAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCTTC1500.11389262203594451Illumina Single End Adapter 2 (100% over 28bp)
ACGTTGGGGTGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTGCTC1490.11313333788903822No Hit
ACGTTGGGGAGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTGCTC1490.11313333788903822No Hit
ACGTTGGGGCTGGCCCTGGTGGTATGGGTGTGGGAGAGCGATGGGCTGAT1490.11313333788903822No Hit
ACGTTGGGTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC1460.11085548544831933No Hit
ACGTTGGGGCCGGCTCTGGTGGCGCAGGCCGGTAGGATTTGCTGAAGGAG1460.11085548544831933No Hit
ACGTTGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.11009620130141301No Hit
ACGTTGGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGA1450.11009620130141301No Hit
ACGTTGGGGGGGTATTAACTTTTATTTGACAGATGACTTGCAAATATGGT1450.11009620130141301No Hit
ACGTTGGGGAGACTTGCTTGGCTTGCCAATAGGATGTCATGATTTTCCAG1430.10857763300760044No Hit
ACGTTGGGGAGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAA1420.10781834886069414No Hit
ACGTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA1420.10781834886069414No Hit
ACGTTGGGGGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAA1410.10705906471378784No Hit
ACGTTGGGGATTCAGGATTTGAAGTCCGCCATCAAAAACTGGTGTTCTTT1400.10629978056688155No Hit
ACGTTGGGGAGTCTTGGCTGAGGCTCTGTGGAAGTCGGCTGTGATTCCGT1380.10478121227306895No Hit
ACGTTGAGGGAGATTTTAGAAAGTGTCTTTAAACGAACTGTCCACAATAT1380.10478121227306895No Hit
ACGTTGGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA1360.10326264397925636Illumina Single End Adapter 2 (100% over 34bp)
ACGTTGACGATGTAGCTTTAGGTGTCTGTAAAACAGGTGCCGAAGAAGCT1360.10326264397925636No Hit
ACGTTGGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATA1350.10250335983235007No Hit
ACGTTGGGGATCTTATTGACCCAGATATATTTTGATCAACGGACCAAGTT1330.10098479153853746No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAA207.8310864E-444.00000416
AGCCTGC207.8310864E-444.00000432
TGTGAGG207.8310864E-444.00000414
ACAGTCC207.8310864E-444.00000435
CAAGATA207.8310864E-444.00000417
GATGGAT207.8310864E-444.00000423
CTCTGAC207.8310864E-444.00000443
ACTAATG207.8310864E-444.00000423
GTAGACG207.8310864E-444.00000421
GTTGACG207.8310864E-444.0000043
GGTGAAA207.8310864E-444.00000422
GAGCGAA207.8310864E-444.00000430
TCCCAGT207.8310864E-444.00000439
CAGTTGA207.8310864E-444.00000442
CCTACGA207.8310864E-444.00000427
GTCACCC207.8310864E-444.00000441
ATCTTGT207.8310864E-444.00000423
CCTACAC207.8310864E-444.00000428
GGCATCC207.8310864E-444.00000413
TACAGTC207.8310864E-444.00000434
Produced by FastQC (version 0.11.5)