FastQCFastQC Report
Fri 17 Jun 2016
SRR1527896_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527896_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161743
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC91875.679998516164533No Hit
ACGTTGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG27321.6890993736977797No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14030.8674254836376226No Hit
ACGTTGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT8730.539745151258478No Hit
ACGTTGGGGGGGCTGGAGAGATGGCTCAGAGGTCAGAGCACTGTCTGTTCTT7960.49213876334679096No Hit
ACGTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT7120.44020452198858684No Hit
ACGTTGGGGACCCACCTAGCCAGAGGTCCCATGTCCTTGGCAGTGAACTCCG6210.3839424271838658No Hit
ACGTTGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCAGTGAGTGCTC5570.34437348138713886No Hit
ACGTTGGGGCGGCATTAGCCGTTCAATCCAGCGCGTACCGATTTTCATCACC5540.34251868705291727No Hit
ACGTTGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTC5390.33324471538180944No Hit
ACGTTGGGGTGGGCTGGCGAGATGGCTCAACGGGTAAGAGCACTGACTGCTC5150.3184063607080368No Hit
ACGTTGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTTT5010.30975065381500283No Hit
ACGTTGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGA4740.29305750480700865No Hit
ACGTTGGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG4730.2924392400289348No Hit
ACGTTGGGGGTTGGCCAGGGAGATCTCCCCGCAAGGGTTGGTCCCTTCCACG4710.29120271047278706No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT4650.28749312180434394No Hit
ACGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4520.2794556796893838No Hit
ACGTTGGGGGGGGCGGTGGTAGCGCACGCCTTTAATCCTAGCACTTGGGAGG4510.2788374149113099No Hit
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACGTTGGGGAAG4220.260907736347168No Hit
ACGTTGGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTC4160.2571981476787249No Hit
ACGTTGGGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGA4150.25657988290065104No Hit
ACGTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGC4090.25287029423220786No Hit
ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3490.21577440754777644No Hit
ACGTTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3460.21391961321355485Illumina Single End Adapter 2 (100% over 34bp)
ACGTTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG3450.213301348435481No Hit
ACGTTGGGGGGGAGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTGCT3360.20773696543281628No Hit
ACGTTGGGGGTGCTACGTTGGGGATGGATGTTTGTGCTCTCTCTGCTCCTGT3210.19846299376170837No Hit
ACGTTGGAGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTC3120.19289861075904366No Hit
ACGTTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3110.19228034598096982Illumina Single End Adapter 2 (100% over 34bp)
ACGTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG3080.19042555164674824No Hit
ACGTTGGGGAATGAAGCATTTAGCTACAGAAATCTAGAGGTCCAAGTCATGT2900.1792967856414188No Hit
ACGTTGGGGGTGCTACGTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAG2890.17867852086334493No Hit
ACGTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA2890.17867852086334493No Hit
ACGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT2850.1762054617510495No Hit
ACGTTGGGGCCTTCTCCCCGGCGGTTAGTGCTGAGAGTGCGGAGTGTGTGCT2850.1762054617510495No Hit
ACGTTGGGGAGGGTTTCAGAAGAAAGAGAGAGATCAGATAGGCCCAGGAACT2840.17558719697297565No Hit
ACGTTGGGGGAGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAA2830.17496893219490178No Hit
ACGTTGGGGCTTCCACCAAGATATCTTGTAAGCTTTGATGTTTTGTATAAAC2780.1718776083045325No Hit
ACGTTGGGGGACTGGGCAGTGGGCCATGCTGTGTGGGCAGGATCAGGGAGGC2710.1675497548580155No Hit
ACGTTGGGGATGTTCAAAAATGAGGACTAAGAACCAAGTTTTGGCACCTTTG2710.1675497548580155No Hit
ACGTTGGGGACAGGGTCTAACCCCACTGTTTAAGAGGCTGTGCTTTATATTG2650.16384016618957234No Hit
ACGTTGGGGTGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAG2610.16136710707727692No Hit
ACGTTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2600.16074884229920305Illumina Single End Adapter 2 (97% over 34bp)
ACGTTGGGGTTCATGACCATGAAACATCCATTTTAAAGCACTGATTTTTGAC2520.15580272407461218No Hit
ACGTTGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2480.15332966496231676Illumina Single End Adapter 2 (97% over 34bp)
ACGTTGGGTATTGGAGAGCAACAGGGAAGGGGCCTCTTCCATCAGCAGAGGC2440.15085660585002134No Hit
ACGTTGGGTGCTGGAGACATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTC2430.15023834107194747No Hit
ACGTTGGGGGCCGGGATTGTGGCGGTCGGTCTCTCGAGTCGGAAGCCGGAGC2420.1496200762938736No Hit
ACGTTGGGGTTGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA2360.14591048762543046No Hit
ACGTTGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTT2350.14529222284735663No Hit
ACGTTGGGGCCTCCTGCTGCAGTCCTGAACTCTCTCCCTCTGATGTGTCCCC2330.1440556932912089No Hit
ACGTTGGGATACTTTTGTTTTTTTATGTATGCATTAGAATGCAGCAATATGC2320.14343742851313504No Hit
ACGTTGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT2310.14281916373506118No Hit
ACGTTGGGGGTGGCCATGAAAACTCCCCTGCCAGGTAAGTACAACACAAAAG2300.14220089895698731No Hit
ACGTTGGGGAAGCATAGCAACAGTGAACATAAAGATTCTGAAAAGAAACACA2300.14220089895698731No Hit
ACGTTGAGGATTTGTGGCTCTTGCAAAGGACACAGTTTCAATTCCAAGTATC2300.14220089895698731No Hit
ACGTTGGGGGTGCTACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA2290.14158263417891345No Hit
ACGTTGGGGTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC2270.14034610462276575No Hit
ACGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2230.1378730455104703No Hit
ACGTTGGGGATTAGCCTAGTTTCGTGAGGCAGAACGACCAGGATAAGATACG2200.13601825117624874No Hit
ACGTTGGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACA2180.13478172162010102No Hit
ACGTTGGGGATGGTATGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCG2170.13416345684202718No Hit
ACGTTGGGGAGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAG2140.1323086625078056No Hit
ACGTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC2130.13169039772973173No Hit
ACGTTGGGGCTGGCCCTGGTGGTATGGGTGTGGGAGAGCGATGGGCTGATCA2080.12859907383936245No Hit
ACGTTGGGGTGCCGGTAGTGATGGCGCACGCCGGTAGGATTTGCTGAAGGAG2030.12550774994899314No Hit
ACGTTGGGTGGAGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTGCTC2010.12427122039284544No Hit
ACGTTGGGGAAGACACAAAGTGGAATTGTTGAAATATAAATGCACTCTAGAA2000.12365295561477159No Hit
ACGTTGGGGGCTCTCCAAAGCGGTTGTGCGTTTTACAGTCCTTTCCAGCTTT1950.1205616317244023No Hit
ACGTTGGGGAGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTGCTCTT1950.1205616317244023No Hit
ACGTTGAGGAGCAAAAAGGAAATCTGTGGATCTCTTAAAGTTATGGGCCAGT1940.11994336694632843No Hit
ACGTTGGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAAC1940.11994336694632843No Hit
ACGTTGGGGGCACTCTCTCCAGTCTCTGTTCTCTCTCCTCCGCGCCCCCCCC1930.11932510216825457No Hit
ACGTTGGGGACCAGGTCAACGTGAGGGGCTTACATTCTGGGTTTGATAAGAA1920.11870683739018072No Hit
ACGTTGGGGTATTCTATTGAAAAGGAATCCTAATAATTCATTGGGTAGGATG1910.11808857261210687No Hit
ACGTTGGGGGCCGGTACTGGTGGCGCAGGCCGGTAGGACTCTGCCTCCCAAG1870.11561551349981143No Hit
ACGTTGGGGGGGTATTAACTTTTATTTGACAGATGACTTGCAAATATGGTCT1870.11561551349981143No Hit
ACGTTGGGGAGACTTGCTTGGCTTGCCAATAGGATGTCATGATTTTCCAGGT1860.11499724872173758No Hit
ACGTTGGGTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC1840.11376071916558986No Hit
ACGTTGGGGACTCGGGTTGTACCAGTGACCGCTGTATGTTACAGCCTTCTAC1830.113142454387516No Hit
ACGTTGGGGGAGTTCCAGACTAGTAAGTGACCTTGTCCCAAATAAAAGGTAG1830.113142454387516No Hit
ACGTTGGGGGCAGAGTGCAGTGCTACGTTGGGGGCTGGTGAGATGGCTCAGT1820.11252418960944215No Hit
ACGTTGGGGAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG1820.11252418960944215Illumina Single End Adapter 2 (100% over 34bp)
ACGTTGGGGAAAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT1820.11252418960944215Illumina Single End Adapter 2 (100% over 32bp)
ACGTTGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA1800.11128766005329442No Hit
ACGTTGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTT1770.10943286571907286No Hit
ACGTTGGGGAGTCTTGGCTGAGGCTCTGTGGAAGTCGGCTGTGATTCCGTGT1730.10695980660677742No Hit
ACGTTGGGGTGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTGCTCTT1720.10634154182870358No Hit
ACGTTGGGGAAAAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGC1720.10634154182870358Illumina Single End Adapter 2 (100% over 31bp)
ACGTTGGGGGGAAATAATACCCTACAGATGAGCGAAAATGTATTATTGTAAA1720.10634154182870358No Hit
ACGTTGGGGAAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTT1690.10448674749448199Illumina Single End Adapter 2 (100% over 33bp)
ACGTTGGGGAGTCCTGATTTACTGAACCAGGGTCTTACAGTGTAACCCAGGC1670.10325021793833426No Hit
ACGTTGGGTGTGTACCAGCACAAATACTGCTTCCTTTGGTCCCCCAGAGCTG1650.10201368838218655No Hit
ACGTTGGGAAGTGTTTGTCTTTTTTTTAGTTTTAGCTTATTGGGATTCTCTT1650.10201368838218655No Hit
ACGTTGGGGCCGGCTCTGGTGGCGCAGGCCGGTAGGATTTGCTGAAGGAGGC1640.1013954236041127No Hit
ACGTTGGGGATCTTATTGACCCAGATATATTTTGATCAACGGACCAAGTTAC1630.10077715882603883No Hit
ACGTTGGGGAACCTGTCAGCTTTTAGAGACAGTAGTAGCTCATCACAGGAGC1630.10077715882603883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATT206.295297E-446.00000421
TGGTCTT206.295297E-446.00000441
TTATGAT206.295297E-446.00000430
GAGAATT206.295297E-446.00000418
CGTCAGG206.295297E-446.00000431
ACTGCGG206.295297E-446.00000411
TTTTGAT206.295297E-446.00000430
GCCTCTG206.295297E-446.00000422
GCCTCTC206.295297E-446.00000422
AAACCAC206.295297E-446.00000436
TTCACAT206.295297E-446.00000427
ACTAAAT206.295297E-446.00000419
TGGCACT206.295297E-446.00000412
GTTTGAA206.295297E-446.00000439
AATCCCA206.295297E-446.00000437
CTGAGGG206.295297E-446.00000435
GTCATGC206.295297E-446.00000411
TATATTG405.5606506E-946.00000446
GTCATCT206.295297E-446.00000439
ATCTCCG206.295297E-446.00000410
Produced by FastQC (version 0.11.5)