FastQCFastQC Report
Fri 17 Jun 2016
SRR1527895_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527895_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163734
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC89725.479619382657237No Hit
ACGTTGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG25691.5690082695103031No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13460.8220650567383683No Hit
ACGTTGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT8460.5166917072813222No Hit
ACGTTGGGGGGGCTGGAGAGATGGCTCAGAGGTCAGAGCACTGTCTGTTCTT7600.4641674911747102No Hit
ACGTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT6360.38843490050936275No Hit
ACGTTGGGGACCCACCTAGCCAGAGGTCCCATGTCCTTGGCAGTGAACTCCG6230.38049519342347954No Hit
ACGTTGGGGCGGCATTAGCCGTTCAATCCAGCGCGTACCGATTTTCATCACC5560.3395751645962354No Hit
ACGTTGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCAGTGAGTGCTC5450.33285695090818035No Hit
ACGTTGGGGTGGGCTGGCGAGATGGCTCAACGGGTAAGAGCACTGACTGCTC5360.32736023061795355No Hit
ACGTTGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTC5070.3096485763494448No Hit
ACGTTGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGA5020.30659484285487437No Hit
ACGTTGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTTT4990.3047626027581321No Hit
ACGTTGGGGGTTGGCCAGGGAGATCTCCCCGCAAGGGTTGGTCCCTTCCACG4690.28644020179070934No Hit
ACGTTGGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTC4600.2809434815004825No Hit
ACGTTGGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG4510.2754467612102557No Hit
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACGTTGGGGAAG4420.26995004092002883No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT4400.26872854752220066No Hit
ACGTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGC4350.2656748140276302No Hit
ACGTTGGGGGGGGCGGTGGTAGCGCACGCCTTTAATCCTAGCACTTGGGAGG4320.2638425739308879No Hit
ACGTTGGGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGA4080.2491846531569497No Hit
ACGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3980.24307718616780877No Hit
ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3650.2229225451036437No Hit
ACGTTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG3560.21742582481341688No Hit
ACGTTGGGGGGGAGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTGCT3260.1991034238459941No Hit
ACGTTGGGGAATGAAGCATTTAGCTACAGAAATCTAGAGGTCCAAGTCATGT3120.1905529700611968No Hit
ACGTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG3050.18627774316879817No Hit
ACGTTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3010.1838347563731418Illumina Single End Adapter 2 (100% over 34bp)
ACGTTGGGGGTGCTACGTTGGGGATGGATGTTTGTGCTCTCTCTGCTCCTGT2970.18139176957748543No Hit
ACGTTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2880.1758950492872586Illumina Single End Adapter 2 (100% over 34bp)
ACGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT2860.17467355588943043No Hit
ACGTTGGGGACAGGGTCTAACCCCACTGTTTAAGAGGCTGTGCTTTATATTG2770.1691768355992036No Hit
ACGTTGGGGAGGGTTTCAGAAGAAAGAGAGAGATCAGATAGGCCCAGGAACT2740.1673445955024613No Hit
ACGTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA2740.1673445955024613No Hit
ACGTTGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTT2720.16612310210463313No Hit
ACGTTGGGGCTTCCACCAAGATATCTTGTAAGCTTTGATGTTTTGTATAAAC2690.16429086200789086No Hit
ACGTTGGGGCCTTCTCCCCGGCGGTTAGTGCTGAGAGTGCGGAGTGTGTGCT2670.16306936861006266No Hit
ACGTTGGAGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTC2660.16245862191114857No Hit
ACGTTGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT2630.16062638181440628No Hit
ACGTTGGGGATGTTCAAAAATGAGGACTAAGAACCAAGTTTTGGCACCTTTG2610.1594048884165781No Hit
ACGTTGGGTGCTGGAGACATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTC2590.15818339501874992No Hit
ACGTTGGGGGTGCTACGTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAG2560.15635115492200763No Hit
ACGTTGAGGATTTGTGGCTCTTGCAAAGGACACAGTTTCAATTCCAAGTATC2560.15635115492200763No Hit
ACGTTGGGGGAGTGCAGTGCTACGTTGGGGAAAAAAAAAAAAAAAAAAAAAA2560.15635115492200763No Hit
ACGTTGGGGGACTGGGCAGTGGGCCATGCTGTGTGGGCAGGATCAGGGAGGC2470.1508544346317808No Hit
ACGTTGGGATACTTTTGTTTTTTTATGTATGCATTAGAATGCAGCAATATGC2420.14780070113721036No Hit
ACGTTGGGGTTGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA2350.1435254742448117No Hit
ACGTTGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2340.14291472754589762Illumina Single End Adapter 2 (97% over 34bp)
ACGTTGGGTATTGGAGAGCAACAGGGAAGGGGCCTCTTCCATCAGCAGAGGC2290.13986099405132715No Hit
ACGTTGGGGTGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAG2280.13925024735241306No Hit
ACGTTGGGGATGGTATGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCG2250.13741800725567077No Hit
ACGTTGGGGAGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAG2220.1355857671589285No Hit
ACGTTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2210.13497502046001442Illumina Single End Adapter 2 (97% over 34bp)
ACGTTGGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACA2190.13375352706218624No Hit
ACGTTGGGGGTGGCCATGAAAACTCCCCTGCCAGGTAAGTACAACACAAAAG2150.13131054026652986No Hit
ACGTTGGGGGTGCTACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA2150.13131054026652986No Hit
ACGTTGGGGCCTCCTGCTGCAGTCCTGAACTCTCTCCCTCTGATGTGTCCCC2100.12825680677195941No Hit
ACGTTGGGGAAGCATAGCAACAGTGAACATAAAGATTCTGAAAAGAAACACA2070.12642456667521712No Hit
ACGTTGGGGTTCATGACCATGAAACATCCATTTTAAAGCACTGATTTTTGAC2060.12581381997630303No Hit
ACGTTGGGGAAGACACAAAGTGGAATTGTTGAAATATAAATGCACTCTAGAA2060.12581381997630303No Hit
ACGTTGGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAAC2050.12520307327738894No Hit
ACGTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC2030.12398157987956075No Hit
ACGTTGGGGGGGTATTAACTTTTATTTGACAGATGACTTGCAAATATGGTCT1950.11909560628824802No Hit
ACGTTGGGGTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC1950.11909560628824802No Hit
ACGTTGGGGCTGGCCCTGGTGGTATGGGTGTGGGAGAGCGATGGGCTGATCA1940.11848485958933393No Hit
ACGTTGGGGGCCGGGATTGTGGCGGTCGGTCTCTCGAGTCGGAAGCCGGAGC1940.11848485958933393No Hit
ACGTTGGGGATTAGCCTAGTTTCGTGAGGCAGAACGACCAGGATAAGATACG1940.11848485958933393No Hit
ACGTTGGGGTATTCTATTGAAAAGGAATCCTAATAATTCATTGGGTAGGATG1940.11848485958933393No Hit
ACGTTGGGGGCAGAGTGCAGTGCTACGTTGGGGGCTGGTGAGATGGCTCAGT1910.11665261949259165No Hit
ACGTTGGGGTGCCGGTAGTGATGGCGCACGCCGGTAGGATTTGCTGAAGGAG1880.11482037939584935No Hit
ACGTTGGGGACCAGGTCAACGTGAGGGGCTTACATTCTGGGTTTGATAAGAA1870.11420963269693526No Hit
ACGTTGAGGAGCAAAAAGGAAATCTGTGGATCTCTTAAAGTTATGGGCCAGT1850.11298813929910707No Hit
ACGTTGGGGATTCAGGATTTGAAGTCCGCCATCAAAAACTGGTGTTCTTTGC1830.1117666459012789No Hit
ACGTTGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTT1770.10810216570779434No Hit
ACGTTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1770.10810216570779434No Hit
ACGTTGGGGAGTCTTGGCTGAGGCTCTGTGGAAGTCGGCTGTGATTCCGTGT1760.10749141900888025No Hit
ACGTTGGGGAAAAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGC1730.10565917891213797Illumina Single End Adapter 2 (100% over 31bp)
ACGTTGGGGAGCTGGTGAGATGGCTCAGCGGTTAAGAGCGCTGACTGCTCTT1730.10565917891213797No Hit
ACGTTGGGGAGACTTGCTTGGCTTGCCAATAGGATGTCATGATTTTCCAGGT1720.10504843221322388No Hit
ACGTTGGGGAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG1720.10504843221322388Illumina Single End Adapter 2 (100% over 34bp)
ACGTTGGGGATCTTATTGACCCAGATATATTTTGATCAACGGACCAAGTTAC1710.10443768551430979No Hit
ACGTTGGGTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC1700.1038269388153957No Hit
ACGTTGGGGAACCTGTCAGCTTTTAGAGACAGTAGTAGCTCATCACAGGAGC1700.1038269388153957No Hit
ACGTTGGGGAAAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT1690.1032161921164816Illumina Single End Adapter 2 (100% over 32bp)
ACGTTGGGGGCCGGTACTGGTGGCGCAGGCCGGTAGGACTCTGCCTCCCAAG1680.10260544541756751No Hit
ACGTTGGGGACTCGGGTTGTACCAGTGACCGCTGTATGTTACAGCCTTCTAC1680.10260544541756751No Hit
ACGTTGGGAAGTGTTTGTCTTTTTTTTAGTTTTAGCTTATTGGGATTCTCTT1670.10199469871865342No Hit
ACGTTGGGGCCGGCTCTGGTGGCGCAGGCCGGTAGGATTTGCTGAAGGAGGC1660.10138395201973933No Hit
ACGTTGGGGGCACTCTCTCCAGTCTCTGTTCTCTCTCCTCCGCGCCCCCCCC1640.10016245862191114No Hit
ACGTTGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGTTTTTTT1640.10016245862191114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGG351.0123949E-746.00000431
ACTAATG351.0123949E-746.00000423
GCCTTGA351.0123949E-746.00000416
CCTACGA351.0123949E-746.00000427
ATCTCCC750.046.00000423
TCGATGA700.046.00000443
TTCAATT351.0123949E-746.00000437
TGCGTCG750.046.00000439
TAAGCTT351.0123949E-746.00000429
AATTCCA351.0123949E-746.00000440
GCGATCC351.0123949E-746.00000425
AGGACAC351.0123949E-746.00000427
TAAGCCG351.0123949E-746.00000418
GTCGATG700.046.00000442
GATAACT351.0123949E-746.00000436
TAATGTA351.0123949E-746.00000425
CAATTCC351.0123949E-746.00000439
CTAAGCC351.0123949E-746.00000417
TCAATTC351.0123949E-746.00000438
CCTTGAC351.0123949E-746.00000417
Produced by FastQC (version 0.11.5)