FastQCFastQC Report
Fri 17 Jun 2016
SRR1527894_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527894_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615856
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT128692.089611857317295No Hit
TCTACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC113101.8364682653087734No Hit
TCTACCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG47050.7639772933932608No Hit
TCTACCGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT30310.49216050505312936No Hit
TCTACCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC27720.45010521940193815No Hit
TCTACCGGGGCTGGCCTGCACTTAAGAGCCCTAGACCCATTTCCTCAAGG25360.4117845730170689No Hit
TCTACCGGGACAAACACCTCAGCCTTCAGAGGAGACTGAGCTCCTCAGAC22610.36713127744147983No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTACCGGGA22200.3604738770102102No Hit
TCTACCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC21630.35121846665454265No Hit
TCTACCGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA17450.28334545737964717No Hit
TCTACCGGGGACAAACACCTCAGCCTTCAGAGGAGACTGAGCTCCTCAGA15270.24794757215972565No Hit
TCTACCAAAGCTGTGCTCAGCCAATTTCAAGCAGATTTGGACAATTTGGA14720.23901691304460781No Hit
TCTACCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG14430.2343080200566366No Hit
TCTACCGGGGCTTACACAGTATGGCCGGCGACATTAGCTAGCGCTCGCTC13510.21936946299134863No Hit
TCTACCGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC12100.19647450053259202No Hit
TCTACCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA11360.1844587046322517No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA9970.16188849341404485No Hit
TCTACCGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT8620.13996778467693746No Hit
TCTACCGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC8520.13834402847418878No Hit
TCTACCGGGCATATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATT8460.13736977475253956No Hit
TCTACCGGGGAGTGCAGTGCTTCTACCGGGGGAGGAGCGGAGGGGCTGGG7860.12762723753604738No Hit
TCTACCGGGGAGAGGAGACTGAGCTCCTCAGACGACAAGAGTGGACCCGT7760.1260034813332987No Hit
TCTACCGGGGGCTGGCCTGCACTTAAGAGCCCTAGACCCATTTCCTCAAG7550.12259359330752645No Hit
TCTACCGGGGTGGACTCAGGGCATGGACGCGACCATCCTCCTCTTAGGAG7390.11999558338312852No Hit
TCTACCGGGGCCATTACTGCCGGAAAAGGTCCAGCAGAGCTCAGCAGCCA7360.11950845652230391No Hit
TCTACCGGGGATAGCAGCAGTGTTCTGGGCAGCTCAGCCTCACGTTGTTC7250.11772232469928035No Hit
TCTACCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTT7100.1152866903951573No Hit
TCTACCGGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGA7000.11366293419240861No Hit
TCTACCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA6910.11220155360993479No Hit
TCTACCGGGGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAAT6420.10424514821646619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGT302.5257941E-644.00000429
CGGGTTA1100.044.0000046
TCGCAAC302.5257941E-644.00000422
CGCGGAT302.5257941E-644.00000421
CAGCATA454.783942E-1044.00000428
CGCGATA454.783942E-1044.00000435
TACGCGA454.783942E-1044.00000433
CGTAGGA650.044.00000416
CATACGG454.783942E-1044.00000436
CTCGCAC454.783942E-1044.00000425
TGTAGTA454.783942E-1044.00000418
ACTCATA454.783942E-1044.00000421
CGTCATT454.783942E-1044.0000046
CGTTTAG302.5257941E-644.00000416
TATCGCT302.5257941E-644.00000422
CGATATC454.783942E-1044.00000437
ATCGCTA302.5257941E-644.00000423
ACGAACG302.5257941E-644.00000444
ACCGGAT4150.044.04
AATTTCC254.4404245E-544.011
Produced by FastQC (version 0.11.5)