FastQCFastQC Report
Fri 17 Jun 2016
SRR1527893_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527893_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920758
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG199672.1685393990603394No Hit
TCTACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC173461.8838826271398128No Hit
TCTACCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT71500.776534116456224No Hit
TCTACCGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC44170.47971345348071914No Hit
TCTACCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT41090.44626275307952795No Hit
TCTACCGGGGCTGGCCTGCACTTAAGAGCCCTAGACCCATTTCCTCAAGGTA36870.40043094928309064No Hit
TCTACCGGGACAAACACCTCAGCCTTCAGAGGAGACTGAGCTCCTCAGACGA35440.3849002669539662No Hit
TCTACCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG31960.3471053197474255No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTACCGGGAAG27390.2974722999963074No Hit
TCTACCGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC25180.2734703363967514No Hit
TCTACCAAAGCTGTGCTCAGCCAATTTCAAGCAGATTTGGACAATTTGGAGA22340.2426261840787699No Hit
TCTACCGGGGACAAACACCTCAGCCTTCAGAGGAGACTGAGCTCCTCAGACG21880.23763030025261794No Hit
TCTACCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC21470.23317744727713471No Hit
TCTACCGGGGCTTACACAGTATGGCCGGCGACATTAGCTAGCGCTCGCTCAA19890.21601767239600414No Hit
TCTACCGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC19030.20667754176450273No Hit
TCTACCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA16700.18137230412334185No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC13800.14987651478455793No Hit
TCTACCGGGCATATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTC13620.14792160372215066No Hit
TCTACCGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG12970.14086220266345773No Hit
TCTACCGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG12640.13727819904904437No Hit
TCTACCGGGGAGTGCAGTGCTTCTACCGGGGGAGGAGCGGAGGGGCTGGGGC12270.13325977075409606No Hit
TCTACCGGGGAGAGGAGACTGAGCTCCTCAGACGACAAGAGTGGACCCGTGT11200.12163891054978615No Hit
TCTACCGGGGATAGCAGCAGTGTTCTGGGCAGCTCAGCCTCACGTTGTTCCC11110.12066145501858253No Hit
TCTACCGGGGCCATTACTGCCGGAAAAGGTCCAGCAGAGCTCAGCAGCCAAG10950.11892375629644271No Hit
TCTACCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA10830.11762048225483787No Hit
TCTACCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT10780.11707745140416917No Hit
TCTACCGGGGGCTGGCCTGCACTTAAGAGCCCTAGACCCATTTCCTCAAGGT10340.11229877991828473No Hit
TCTACCGGGGTGGACTCAGGGCATGGACGCGACCATCCTCCTCTTAGGAGTG10220.11099550587667986No Hit
TCTACCGGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACA9860.10708568375186532No Hit
TCTACCGGGAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTGG9370.10176398141531218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG206.310796E-446.00000439
GTACCGT206.310796E-446.00000446
CGAACCC206.310796E-446.00000429
CCGATTA206.310796E-446.00000427
CGACGCC206.310796E-446.00000436
ACATTCG206.310796E-446.00000425
CGTCTCG405.6097633E-946.00000442
ATTCGCA206.310796E-446.00000427
GTAGGTA206.310796E-446.00000410
TAGTTCG206.310796E-446.00000437
ATCCGAT405.6097633E-946.00000411
TAGCACG206.310796E-446.00000428
CGTAGTA206.310796E-446.00000441
CGAATTC206.310796E-446.00000434
TGTTACG206.310796E-446.00000430
CGAATCT206.310796E-446.00000444
CCCCATA206.310796E-446.00000429
GCGATAC206.310796E-446.0000049
TACTCGA206.310796E-446.00000444
CTATTAC405.6097633E-946.00000420
Produced by FastQC (version 0.11.5)