##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527893_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	920758
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36207342211526	33.0	31.0	34.0	31.0	34.0
2	32.64360885270614	34.0	31.0	34.0	31.0	34.0
3	32.69122179769277	34.0	31.0	34.0	31.0	34.0
4	36.352252166150066	37.0	37.0	37.0	35.0	37.0
5	36.25862387293947	37.0	37.0	37.0	35.0	37.0
6	36.309727420234196	37.0	37.0	37.0	35.0	37.0
7	36.46905049969699	37.0	37.0	37.0	35.0	37.0
8	36.601670580108994	37.0	37.0	37.0	35.0	37.0
9	38.62737548845625	39.0	39.0	39.0	38.0	39.0
10	38.01439248966612	39.0	38.0	39.0	35.0	39.0
11	37.71733289311632	39.0	38.0	39.0	35.0	39.0
12	37.46904941363529	39.0	37.0	39.0	35.0	39.0
13	37.389918958075846	39.0	37.0	39.0	35.0	39.0
14	38.26712773606094	40.0	38.0	40.0	35.0	40.0
15	38.31142167648829	40.0	38.0	40.0	35.0	40.0
16	38.32023941144144	40.0	38.0	40.0	35.0	40.0
17	38.30490096203346	40.0	38.0	40.0	35.0	40.0
18	38.2805710078001	40.0	38.0	40.0	35.0	40.0
19	38.198525562634266	40.0	38.0	40.0	34.0	40.0
20	38.19875797983835	40.0	38.0	40.0	34.0	40.0
21	38.15600516096521	40.0	38.0	40.0	34.0	40.0
22	38.12589953060413	40.0	38.0	40.0	34.0	40.0
23	38.037514743287595	40.0	38.0	40.0	34.0	40.0
24	37.96821531824866	40.0	38.0	40.0	34.0	40.0
25	37.84735620000043	40.0	37.0	40.0	34.0	40.0
26	37.66008658083883	40.0	37.0	40.0	33.0	40.0
27	37.55447794100078	40.0	37.0	40.0	33.0	40.0
28	37.34396985961566	40.0	37.0	40.0	33.0	40.0
29	37.24742657679868	40.0	36.0	40.0	33.0	40.0
30	37.18733478286369	40.0	36.0	40.0	33.0	40.0
31	37.0296418820146	39.0	36.0	40.0	32.0	40.0
32	36.754798763627356	39.0	35.0	40.0	31.0	40.0
33	36.62440293757969	39.0	35.0	40.0	31.0	40.0
34	36.504357279545765	39.0	35.0	40.0	31.0	40.0
35	36.36035310038034	39.0	35.0	40.0	31.0	40.0
36	36.228127260365916	39.0	35.0	40.0	30.0	40.0
37	36.00655655449097	38.0	35.0	40.0	30.0	40.0
38	35.94719350795757	38.0	35.0	40.0	30.0	40.0
39	35.778029623418966	38.0	35.0	40.0	30.0	40.0
40	35.50943570406122	38.0	35.0	40.0	28.0	40.0
41	35.336034006763995	38.0	34.0	40.0	28.0	40.0
42	35.20751163715113	38.0	34.0	40.0	28.0	40.0
43	35.02640215995951	37.0	34.0	40.0	27.0	40.0
44	34.8936973667348	37.0	34.0	40.0	27.0	40.0
45	34.77840322864422	37.0	34.0	40.0	27.0	40.0
46	34.509041463663635	36.0	34.0	40.0	26.0	40.0
47	34.271444831323755	36.0	33.0	39.0	26.0	40.0
48	33.91622011429713	35.0	33.0	39.0	24.0	40.0
49	34.1596565004051	35.0	33.0	39.0	26.0	40.0
50	34.200468527017954	35.0	33.0	39.0	26.0	40.0
51	34.12580069898931	35.0	33.0	39.0	26.0	40.0
52	33.60705744614763	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	4.0
15	10.0
16	25.0
17	45.0
18	141.0
19	337.0
20	660.0
21	1064.0
22	1952.0
23	3024.0
24	4294.0
25	5935.0
26	8179.0
27	10831.0
28	12735.0
29	14448.0
30	15964.0
31	19267.0
32	24241.0
33	31843.0
34	48768.0
35	66232.0
36	101839.0
37	173194.0
38	259472.0
39	116252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	98.79012726471016	0.8705870597920409	0.09101197057207214	0.24827370492572423
8	97.97699286891887	1.5397096739859986	0.26467323661591863	0.21862422047921387
9	94.25712293566822	3.6570955669133474	0.906970126786843	1.1788113706315884
10	48.52534542192411	35.27408939156651	7.080362049528757	9.120203136980617
11	29.341260135670826	34.15327371578634	19.027040764239896	17.478425384302934
12	25.9388460377211	24.400113819266302	25.743463537650502	23.917576605362104
13	22.787529405120562	22.54707534444447	26.9779898735607	27.68740537687427
14	22.556089656565568	25.21194494101599	24.597559836569435	27.634405565849008
15	24.59451886380569	24.476681169210586	23.981545639570875	26.94725432741285
16	27.086487437524298	24.51284702386512	22.638738952037343	25.761926586573235
17	27.387760953475286	25.185336429333223	20.151983474485153	27.274919142706334
18	27.36984093540322	25.205754389318365	22.466598172375367	24.957806502903043
19	28.76304088587881	21.63641260787308	22.38872754838948	27.211818957858636
20	27.246355719961162	22.46171089471935	22.706509202200795	27.58542418311869
21	30.387680584909386	20.908642661806905	24.67586488523586	24.027811868047845
22	27.349640187758347	20.737805156186536	22.917856809281048	28.99469784677407
23	24.34548491568903	24.716157774355477	24.83975159596767	26.098605713987823
24	25.31892201859772	22.9399038618182	27.91222014905111	23.82895397053297
25	23.447746313363556	24.89405468103454	24.74537283412145	26.912826171480454
26	25.42068600001303	24.789032514515213	23.891076699849474	25.89920478562228
27	25.720221817241878	22.0994007111532	25.000380121595466	27.17999735000945
28	28.81093620690779	25.526577015893427	22.965752130310026	22.696734646888757
29	28.73773564823765	24.86245028552562	20.951650705179865	25.44816336105687
30	28.238147265622455	23.76813451525808	20.92167540222295	27.072042816896513
31	28.50325492691891	26.58820232895071	19.352424849960574	25.556117894169805
32	28.70200421826365	26.20112993859407	21.096965760818804	23.999900082323478
33	28.153325846748007	26.804220001346717	20.460424997664965	24.582029154240313
34	26.85743702471225	26.09969177568916	23.879238627304893	23.163632572293697
35	25.7882092797456	26.40737305567804	24.38034749630196	23.4240701682744
36	25.711641929801317	27.29511989035121	21.49750531627203	25.495732863575444
37	27.199763672973788	25.471079262955087	22.58639077803288	24.742766286038243
38	27.87160144142109	26.144871942464796	22.03249931035082	23.951027305763294
39	26.85396162726797	26.091763525269396	20.956320770495612	26.09795407696702
40	25.22541210611257	23.75759971675511	25.45402809424409	25.56296008288823
41	24.181055174106554	23.704274087219442	26.050384574448444	26.064286164225564
42	24.201907558772227	20.710219188972566	26.362518707412807	28.725354544842403
43	22.371459167338216	22.794806018519523	28.010291520681875	26.823443293460386
44	22.244389948281743	23.23205445947795	25.008416978185366	29.51513861405494
45	24.09297557012809	22.31292044163613	26.91423805169219	26.679865936543585
46	23.26941498200396	21.598183235986003	25.00591903627229	30.126482745737746
47	26.121956040566573	23.108677850206025	23.305580836658493	27.463785272568902
48	27.996172718564488	23.004633139217905	25.205102752297563	23.794091389920045
49	25.696219853642326	22.506239424474185	26.828764995796938	24.96877572608655
50	24.032156114853198	24.533264983850263	24.579531212327236	26.855047688969307
51	26.09534752888381	24.75721090666603	23.407562030414073	25.739879534036085
52	27.283281817806632	24.66543869290302	22.84628534316292	25.20499414612743
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	11.0
6	22.0
7	80.0
8	138.0
9	144.0
10	150.0
11	555.5
12	961.0
13	1022.0
14	1109.0
15	1135.0
16	1132.0
17	1129.0
18	1468.5
19	1808.0
20	1833.5
21	1859.0
22	2443.5
23	3028.0
24	3842.5
25	4657.0
26	5707.5
27	6758.0
28	6988.5
29	7219.0
30	8478.0
31	9737.0
32	11291.5
33	12846.0
34	12602.0
35	12358.0
36	14300.5
37	16243.0
38	17964.5
39	24524.0
40	29362.0
41	27667.5
42	25973.0
43	27102.0
44	28231.0
45	30285.5
46	32340.0
47	34833.0
48	37326.0
49	36717.5
50	36109.0
51	42091.0
52	48073.0
53	49285.0
54	50497.0
55	58114.0
56	65731.0
57	71119.0
58	76507.0
59	98584.0
60	120661.0
61	93522.0
62	66383.0
63	57902.5
64	47466.5
65	45511.0
66	40668.0
67	35825.0
68	28164.0
69	20503.0
70	19313.0
71	18123.0
72	15308.0
73	12493.0
74	10858.0
75	9223.0
76	8379.0
77	7535.0
78	4795.0
79	2055.0
80	1760.5
81	1466.0
82	943.5
83	421.0
84	255.0
85	89.0
86	70.5
87	52.0
88	37.5
89	13.5
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	920758.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.864310221243164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.35099976175003	8.077723266712288
2	8.971999971250169	1.9494918198529314
3	3.746000307726734	1.2209312829604677
4	1.8829999946734048	0.8182998435492438
5	1.0989999964801862	0.5969938447452944
6	0.6559999978979216	0.427619248937873
7	0.47399999848111674	0.36047781198573486
8	0.3199999989745936	0.2781263407725983
9	0.2629999991572441	0.25715822211278916
>10	3.507999988758982	10.315662522014927
>50	2.5829999917230473	20.365366730666057
>100	2.0429999934534213	39.77021979774987
>500	0.07399999976287477	5.254197692361094
>1k	0.024999999919890124	5.47713333738663
>5k	9.99999996795605E-4	0.776798178329718
>10k+	0.00199999999359121	4.053800059862485
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	19967	2.1685393990603394	No Hit
TCTACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	17346	1.8838826271398128	No Hit
TCTACCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	7150	0.776534116456224	No Hit
TCTACCGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	4417	0.47971345348071914	No Hit
TCTACCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	4109	0.44626275307952795	No Hit
TCTACCGGGGCTGGCCTGCACTTAAGAGCCCTAGACCCATTTCCTCAAGGTA	3687	0.40043094928309064	No Hit
TCTACCGGGACAAACACCTCAGCCTTCAGAGGAGACTGAGCTCCTCAGACGA	3544	0.3849002669539662	No Hit
TCTACCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	3196	0.3471053197474255	No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTACCGGGAAG	2739	0.2974722999963074	No Hit
TCTACCGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC	2518	0.2734703363967514	No Hit
TCTACCAAAGCTGTGCTCAGCCAATTTCAAGCAGATTTGGACAATTTGGAGA	2234	0.2426261840787699	No Hit
TCTACCGGGGACAAACACCTCAGCCTTCAGAGGAGACTGAGCTCCTCAGACG	2188	0.23763030025261794	No Hit
TCTACCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	2147	0.23317744727713471	No Hit
TCTACCGGGGCTTACACAGTATGGCCGGCGACATTAGCTAGCGCTCGCTCAA	1989	0.21601767239600414	No Hit
TCTACCGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1903	0.20667754176450273	No Hit
TCTACCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1670	0.18137230412334185	No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	1380	0.14987651478455793	No Hit
TCTACCGGGCATATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTC	1362	0.14792160372215066	No Hit
TCTACCGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1297	0.14086220266345773	No Hit
TCTACCGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG	1264	0.13727819904904437	No Hit
TCTACCGGGGAGTGCAGTGCTTCTACCGGGGGAGGAGCGGAGGGGCTGGGGC	1227	0.13325977075409606	No Hit
TCTACCGGGGAGAGGAGACTGAGCTCCTCAGACGACAAGAGTGGACCCGTGT	1120	0.12163891054978615	No Hit
TCTACCGGGGATAGCAGCAGTGTTCTGGGCAGCTCAGCCTCACGTTGTTCCC	1111	0.12066145501858253	No Hit
TCTACCGGGGCCATTACTGCCGGAAAAGGTCCAGCAGAGCTCAGCAGCCAAG	1095	0.11892375629644271	No Hit
TCTACCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA	1083	0.11762048225483787	No Hit
TCTACCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT	1078	0.11707745140416917	No Hit
TCTACCGGGGGCTGGCCTGCACTTAAGAGCCCTAGACCCATTTCCTCAAGGT	1034	0.11229877991828473	No Hit
TCTACCGGGGTGGACTCAGGGCATGGACGCGACCATCCTCCTCTTAGGAGTG	1022	0.11099550587667986	No Hit
TCTACCGGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACA	986	0.10708568375186532	No Hit
TCTACCGGGAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTGG	937	0.10176398141531218	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.008797099780832748	0.0	0.0	0.0	0.0
8	0.018028624242200447	0.0	0.0	0.0	0.0
9	0.0210695970059451	0.0	0.0	0.0	0.0
10	0.03627446082466837	0.0	0.0	0.0	0.0
11	0.053868660386333866	0.0	0.0	0.0	0.0
12	0.07613292526375008	0.0	0.0	0.0	0.0
13	0.09861440248143377	0.0	0.0	0.0	0.0
14	0.12706921905647303	0.0	0.0	0.0	0.0
15	0.15921664541605937	0.0	0.0	0.0	0.0
16	0.2011386270876821	0.0	0.0	0.0	0.0
17	0.23589260153047814	0.0	0.0	0.0	0.0
18	0.2659765106575235	0.0	0.0	0.0	0.0
19	0.2961690259547025	0.0	0.0	0.0	0.0
20	0.32647014742201536	0.0	0.0	0.0	0.0
21	0.35742290591013054	0.0	0.0	0.0	0.0
22	0.38881008907878073	0.0	0.0	0.0	0.0
23	0.4267136424554552	0.0	0.0	0.0	0.0
24	0.47048192901935143	0.0	0.0	0.0	0.0
25	0.5048014787816125	0.0	0.0	0.0	0.0
26	0.551393525768986	0.0	0.0	0.0	0.0
27	0.5863647125520495	0.0	0.0	0.0	0.0
28	0.6407764037890521	0.0	0.0	0.0	0.0
29	0.6619546069651309	0.0	0.0	0.0	0.0
30	0.690083605029769	0.0	0.0	0.0	0.0
31	0.7171265413930696	0.0	0.0	0.0	0.0
32	0.7502514232838596	0.0	0.0	0.0	0.0
33	0.775339448584753	0.0	0.0	0.0	0.0
34	0.8036856589896585	0.0	0.0	0.0	0.0
35	0.8509293429978344	0.0	0.0	0.0	0.0
36	0.8726505770245819	0.0	0.0	0.0	0.0
37	0.8979558146657428	0.0	0.0	0.0	0.0
38	0.9235868708173048	0.0	0.0	0.0	0.0
39	0.9432445876115114	0.0	0.0	0.0	0.0
40	0.962467879725183	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	20	6.310796E-4	46.000004	39
GTACCGT	20	6.310796E-4	46.000004	46
CGAACCC	20	6.310796E-4	46.000004	29
CCGATTA	20	6.310796E-4	46.000004	27
CGACGCC	20	6.310796E-4	46.000004	36
ACATTCG	20	6.310796E-4	46.000004	25
CGTCTCG	40	5.6097633E-9	46.000004	42
ATTCGCA	20	6.310796E-4	46.000004	27
GTAGGTA	20	6.310796E-4	46.000004	10
TAGTTCG	20	6.310796E-4	46.000004	37
ATCCGAT	40	5.6097633E-9	46.000004	11
TAGCACG	20	6.310796E-4	46.000004	28
CGTAGTA	20	6.310796E-4	46.000004	41
CGAATTC	20	6.310796E-4	46.000004	34
TGTTACG	20	6.310796E-4	46.000004	30
CGAATCT	20	6.310796E-4	46.000004	44
CCCCATA	20	6.310796E-4	46.000004	29
GCGATAC	20	6.310796E-4	46.000004	9
TACTCGA	20	6.310796E-4	46.000004	44
CTATTAC	40	5.6097633E-9	46.000004	20
>>END_MODULE