FastQCFastQC Report
Fri 17 Jun 2016
SRR1527892_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527892_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925726
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG186922.019171979613838No Hit
TCTACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC162531.7557030914114975No Hit
TCTACCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT67700.7313179061622985No Hit
TCTACCGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC42000.4536979624640552No Hit
TCTACCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT39260.42409957157949546No Hit
TCTACCGGGGCTGGCCTGCACTTAAGAGCCCTAGACCCATTTCCTCAAGGTA34380.37138418927414807No Hit
TCTACCGGGACAAACACCTCAGCCTTCAGAGGAGACTGAGCTCCTCAGACGA33310.35982569356375427No Hit
TCTACCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG30310.32741869624489317No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTACCGGGAAG25590.2764316871298851No Hit
TCTACCGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC25570.2762156404810927No Hit
TCTACCAAAGCTGTGCTCAGCCAATTTCAAGCAGATTTGGACAATTTGGAGA21430.23149398418106434No Hit
TCTACCGGGGACAAACACCTCAGCCTTCAGAGGAGACTGAGCTCCTCAGACG20850.22522863136608456No Hit
TCTACCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC20000.21604664879240726No Hit
TCTACCGGGGCTTACACAGTATGGCCGGCGACATTAGCTAGCGCTCGCTCAA18660.20157152332331596No Hit
TCTACCGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC18140.19595431045471337No Hit
TCTACCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA14800.15987452010638137No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC14010.15134067747908128No Hit
TCTACCGGGCATATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTC12860.13891799517351786No Hit
TCTACCGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG12310.13297671233172667No Hit
TCTACCGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG11940.12897984932906714No Hit
TCTACCGGGGAGTGCAGTGCTTCTACCGGGGGAGGAGCGGAGGGGCTGGGGC11940.12897984932906714No Hit
TCTACCGGGGCCATTACTGCCGGAAAAGGTCCAGCAGAGCTCAGCAGCCAAG11360.12271449651408732No Hit
TCTACCGGGGAGAGGAGACTGAGCTCCTCAGACGACAAGAGTGGACCCGTGT11270.12174228659452149No Hit
TCTACCGGGGATAGCAGCAGTGTTCTGGGCAGCTCAGCCTCACGTTGTTCCC10740.1160170504015227No Hit
TCTACCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA10330.11158809410127835No Hit
TCTACCGGGGGCTGGCCTGCACTTAAGAGCCCTAGACCCATTTCCTCAAGGT10300.11126402412808974No Hit
TCTACCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT10080.10888751099137327No Hit
TCTACCGGGGTGGACTCAGGGCATGGACGCGACCATCCTCCTCTTAGGAGTG9810.10597088123267577No Hit
TCTACCGGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACA9690.10467460133992132No Hit
TCTACCGGGAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTGG9400.1015419249324314No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTA206.3108135E-446.00000434
TCGCAAG206.3108135E-446.00000429
CTCCGAA206.3108135E-446.00000427
ACGCATA405.6097633E-946.00000440
TAGGTGC206.3108135E-446.00000434
CGCATAA405.6097633E-946.00000441
CGCGCGA206.3108135E-446.00000415
GATTTCG206.3108135E-446.00000411
CACGACC206.3108135E-446.00000414
TCTAGTT206.3108135E-446.00000432
CGTAGTA206.3108135E-446.00000441
CTACGTA206.3108135E-446.00000431
AATTGCG206.3108135E-446.00000439
AGTTGCG206.3108135E-446.00000436
TCGCTTA206.3108135E-446.00000415
GTCGTAA351.0192889E-746.00000435
CATACGG700.046.00000436
GCGATAC206.3108135E-446.0000049
ACTCGCT206.3108135E-446.00000446
TCGTAAC405.6097633E-946.00000436
Produced by FastQC (version 0.11.5)