##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527892_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	925726
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31833609512966	33.0	31.0	34.0	31.0	34.0
2	32.614097475927004	34.0	31.0	34.0	31.0	34.0
3	32.686505510269775	34.0	31.0	34.0	31.0	34.0
4	36.33911438157727	37.0	37.0	37.0	35.0	37.0
5	36.26770340251867	37.0	37.0	37.0	35.0	37.0
6	36.26761590362591	37.0	37.0	37.0	35.0	37.0
7	36.43195178702985	37.0	37.0	37.0	35.0	37.0
8	36.57646863110683	37.0	37.0	37.0	35.0	37.0
9	38.58611079304244	39.0	39.0	39.0	38.0	39.0
10	37.92480712435429	39.0	38.0	39.0	35.0	39.0
11	37.6903025301223	39.0	38.0	39.0	35.0	39.0
12	37.42241872865189	39.0	37.0	39.0	35.0	39.0
13	37.194655870095474	39.0	37.0	39.0	34.0	39.0
14	38.206091219216056	40.0	38.0	40.0	34.0	40.0
15	38.26481053789134	40.0	38.0	40.0	35.0	40.0
16	38.29882276181073	40.0	38.0	40.0	35.0	40.0
17	38.322847149156445	40.0	38.0	40.0	35.0	40.0
18	38.31299326150502	40.0	38.0	40.0	35.0	40.0
19	38.18960902037968	40.0	38.0	40.0	34.0	40.0
20	38.18526432227247	40.0	38.0	40.0	34.0	40.0
21	38.21009780431791	40.0	38.0	40.0	34.0	40.0
22	38.064397024605555	40.0	38.0	40.0	34.0	40.0
23	38.00019336175067	40.0	38.0	40.0	34.0	40.0
24	37.986882727718566	40.0	38.0	40.0	34.0	40.0
25	37.82858534814837	40.0	37.0	40.0	34.0	40.0
26	37.59181334433731	40.0	37.0	40.0	33.0	40.0
27	37.48617733541026	40.0	37.0	40.0	33.0	40.0
28	37.29813249276784	40.0	36.0	40.0	33.0	40.0
29	37.19964006628311	40.0	36.0	40.0	33.0	40.0
30	37.13178413483039	39.0	36.0	40.0	32.0	40.0
31	36.996048506793585	39.0	36.0	40.0	32.0	40.0
32	36.72031681080579	39.0	35.0	40.0	31.0	40.0
33	36.43305038423897	39.0	35.0	40.0	31.0	40.0
34	36.405610299375844	39.0	35.0	40.0	30.0	40.0
35	36.33762690040034	39.0	35.0	40.0	30.0	40.0
36	36.05652320449031	38.0	35.0	40.0	30.0	40.0
37	35.90606399733831	38.0	35.0	40.0	30.0	40.0
38	35.584208502299816	38.0	35.0	40.0	28.0	40.0
39	35.592008866554465	38.0	35.0	40.0	29.0	40.0
40	35.41111408775383	38.0	35.0	40.0	28.0	40.0
41	35.02978311076928	38.0	34.0	40.0	27.0	40.0
42	35.05114256270214	37.0	34.0	40.0	27.0	40.0
43	34.98191689549608	37.0	34.0	40.0	27.0	40.0
44	34.84495736319386	37.0	34.0	40.0	27.0	40.0
45	34.73157824237409	37.0	34.0	40.0	27.0	40.0
46	34.29638467537911	36.0	33.0	40.0	26.0	40.0
47	33.97525077614758	36.0	33.0	39.0	24.0	40.0
48	33.79111853831479	35.0	33.0	39.0	24.0	40.0
49	33.68048429016793	35.0	33.0	39.0	24.0	40.0
50	33.488989182544294	35.0	32.0	39.0	24.0	40.0
51	33.71134871441441	35.0	33.0	39.0	24.0	40.0
52	33.41856985760366	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	4.0
15	8.0
16	28.0
17	63.0
18	145.0
19	307.0
20	595.0
21	1156.0
22	1964.0
23	3189.0
24	4364.0
25	6056.0
26	8575.0
27	11173.0
28	13139.0
29	14612.0
30	16816.0
31	20240.0
32	25837.0
33	33716.0
34	51213.0
35	69705.0
36	109163.0
37	180329.0
38	252034.0
39	101293.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	98.79791644611905	0.85619286916431	0.09452040884667817	0.25137027586996585
8	97.97272627105644	1.5436533056217498	0.26498121474388747	0.21863920857791613
9	94.23155447724272	3.6508642946184935	0.9221951203703903	1.1953861077683894
10	48.63264075979286	35.17552709981139	7.102317532401596	9.089514607994158
11	29.421124609225625	34.128889109736576	18.96954390391973	17.480442377118067
12	26.11366646286266	24.304167755901855	25.663101176806098	23.919064604429387
13	23.09959966555979	22.538202448672717	26.801667015942083	27.56053086982541
14	22.50687568459782	25.16846237439588	24.708715105765634	27.615946835240667
15	24.756029321851177	24.35861151139754	24.045775963946138	26.83958320280515
16	27.046015775726296	24.498177646517437	22.585732711407047	25.870073866349223
17	27.412538915402614	25.144697243028713	20.208463411419793	27.234300430148878
18	27.374082611917565	25.092089884047763	22.56402002320341	24.96980748083126
19	28.73063951968509	21.553137753503737	22.369902109263432	27.346320617547743
20	27.205674249183886	22.502554751621968	22.665237878162653	27.626533121031493
21	30.315233665253	20.90629408701927	24.680304971449434	24.098167276278293
22	27.49085582558986	20.71671315270393	22.964570510064533	28.827860511641674
23	24.457020759922482	24.717573018366128	24.803559584585503	26.02184663712589
24	25.43603614892528	22.78395551167408	27.922192959903903	23.857815379496742
25	23.51106050818493	24.83261785884808	24.65967251648976	26.99664911647723
26	25.464770353214668	24.7809827097867	23.90275308244556	25.851493854553077
27	25.793701376001106	21.969567669051102	25.03721403525449	27.199516919693302
28	28.826024115126934	25.487671297986665	22.90775024143213	22.77855434545427
29	28.79469735105204	24.80139911809758	20.954256443051182	25.4496470877992
30	28.23103164435265	23.80121115751313	20.868053830183015	27.099703367951207
31	28.53230869609366	26.623104460715158	19.31651482188034	25.528072021310845
32	28.674683437647857	26.22385025374679	21.07448640310416	24.026979905501193
33	28.12948971942022	26.735664764736	20.529940824822894	24.604904691020884
34	26.828672847041133	26.018821984042795	23.972968243303093	23.17953692561298
35	25.882172478681596	26.375947094496645	24.387993855633308	23.35388657118845
36	25.95757275911015	27.222957981087276	21.478277589697168	25.341191670105413
37	27.265411147574987	25.431607192625034	22.602584350012854	24.700397309787128
38	27.87228618403286	26.112154136321113	21.903457394520625	24.112102285125403
39	26.909582317013893	26.015905354284097	20.893331288091723	26.18118104061029
40	25.191687389141066	23.776905909523986	25.48259420174004	25.548812499594913
41	24.405925727483076	23.734020649738692	26.006399301737233	25.853654321041
42	24.278242158046766	20.77569388782426	26.233896422915638	28.71216753121334
43	22.280242750014583	22.87242661435457	27.95902891352301	26.888301722107837
44	22.312541724009048	23.211727876283046	25.057306373592187	29.41842402611572
45	24.13133043686793	22.315458353767745	26.873826596638743	26.67938461272558
46	23.417836379231	21.580359631251582	24.98838749262741	30.01341649689001
47	26.374758837928287	23.184938091832787	23.148858301484456	27.29144476875447
48	27.980417531753453	23.00227065027881	25.1223364148787	23.894975403089035
49	25.801803125330817	22.472956360737413	26.69331962157269	25.03192089235908
50	23.940777292633026	24.60900957734794	24.55175721541795	26.898455914601083
51	26.030056409780002	24.74133814973329	23.403793347059498	25.824812093427212
52	27.3304411888615	24.539982673058766	22.850821949475332	25.278754188604402
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	11.5
6	23.0
7	75.0
8	127.0
9	148.5
10	170.0
11	565.0
12	960.0
13	1011.0
14	1075.0
15	1088.0
16	1123.0
17	1158.0
18	1431.5
19	1705.0
20	1803.0
21	1901.0
22	2420.0
23	2939.0
24	3833.5
25	4728.0
26	5652.5
27	6577.0
28	6866.5
29	7156.0
30	8353.5
31	9551.0
32	11102.0
33	12653.0
34	12587.0
35	12521.0
36	14423.5
37	16326.0
38	18199.0
39	24570.5
40	29069.0
41	27551.0
42	26033.0
43	27344.5
44	28656.0
45	30608.5
46	32561.0
47	34907.0
48	37253.0
49	36944.5
50	36636.0
51	42337.0
52	48038.0
53	49644.5
54	51251.0
55	58381.0
56	65511.0
57	71590.5
58	77670.0
59	98845.0
60	120020.0
61	93842.5
62	67665.0
63	58743.5
64	47902.0
65	45982.0
66	41082.5
67	36183.0
68	28493.5
69	20804.0
70	19500.5
71	18197.0
72	15369.0
73	12541.0
74	10982.5
75	9424.0
76	8455.0
77	7486.0
78	4777.0
79	2068.0
80	1804.0
81	1540.0
82	975.0
83	410.0
84	240.0
85	70.0
86	79.5
87	89.0
88	56.0
89	12.5
90	2.0
91	2.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	925726.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.399993304679837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.61784007640247	11.176963774239058
2	8.947897428258042	2.576993261157555
3	3.3571136115649622	1.4502724058875498
4	1.625045112847438	0.9360255497922317
5	0.8764160123670912	0.6310192355100157
6	0.5024300803368847	0.4340993875752535
7	0.3525465473123223	0.3553667544619804
8	0.29010306294762256	0.3341985731290303
9	0.19464380393194336	0.25225825260756596
>10	2.798594707765372	10.825736725944347
>50	1.9433838374515713	20.200180529975658
>100	1.4233445429749714	36.50057317385198
>500	0.05035062533227196	4.71032736682426
>1k	0.018036044895142194	5.101745431147631
>5k	7.515018706309248E-4	0.7326238030050369
>10k+	0.0015030037412618496	3.781615774890844
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	18692	2.019171979613838	No Hit
TCTACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	16253	1.7557030914114975	No Hit
TCTACCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	6770	0.7313179061622985	No Hit
TCTACCGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	4200	0.4536979624640552	No Hit
TCTACCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	3926	0.42409957157949546	No Hit
TCTACCGGGGCTGGCCTGCACTTAAGAGCCCTAGACCCATTTCCTCAAGGTA	3438	0.37138418927414807	No Hit
TCTACCGGGACAAACACCTCAGCCTTCAGAGGAGACTGAGCTCCTCAGACGA	3331	0.35982569356375427	No Hit
TCTACCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	3031	0.32741869624489317	No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTACCGGGAAG	2559	0.2764316871298851	No Hit
TCTACCGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC	2557	0.2762156404810927	No Hit
TCTACCAAAGCTGTGCTCAGCCAATTTCAAGCAGATTTGGACAATTTGGAGA	2143	0.23149398418106434	No Hit
TCTACCGGGGACAAACACCTCAGCCTTCAGAGGAGACTGAGCTCCTCAGACG	2085	0.22522863136608456	No Hit
TCTACCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	2000	0.21604664879240726	No Hit
TCTACCGGGGCTTACACAGTATGGCCGGCGACATTAGCTAGCGCTCGCTCAA	1866	0.20157152332331596	No Hit
TCTACCGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1814	0.19595431045471337	No Hit
TCTACCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1480	0.15987452010638137	No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	1401	0.15134067747908128	No Hit
TCTACCGGGCATATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTC	1286	0.13891799517351786	No Hit
TCTACCGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1231	0.13297671233172667	No Hit
TCTACCGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG	1194	0.12897984932906714	No Hit
TCTACCGGGGAGTGCAGTGCTTCTACCGGGGGAGGAGCGGAGGGGCTGGGGC	1194	0.12897984932906714	No Hit
TCTACCGGGGCCATTACTGCCGGAAAAGGTCCAGCAGAGCTCAGCAGCCAAG	1136	0.12271449651408732	No Hit
TCTACCGGGGAGAGGAGACTGAGCTCCTCAGACGACAAGAGTGGACCCGTGT	1127	0.12174228659452149	No Hit
TCTACCGGGGATAGCAGCAGTGTTCTGGGCAGCTCAGCCTCACGTTGTTCCC	1074	0.1160170504015227	No Hit
TCTACCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA	1033	0.11158809410127835	No Hit
TCTACCGGGGGCTGGCCTGCACTTAAGAGCCCTAGACCCATTTCCTCAAGGT	1030	0.11126402412808974	No Hit
TCTACCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT	1008	0.10888751099137327	No Hit
TCTACCGGGGTGGACTCAGGGCATGGACGCGACCATCCTCCTCTTAGGAGTG	981	0.10597088123267577	No Hit
TCTACCGGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACA	969	0.10467460133992132	No Hit
TCTACCGGGAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTGG	940	0.1015419249324314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.007129539410149439	0.0	0.0	0.0	0.0
8	0.015123265415468507	0.0	0.0	0.0	0.0
9	0.01879605844493943	0.0	0.0	0.0	0.0
10	0.03305513726523831	0.0	0.0	0.0	0.0
11	0.05033886916863089	0.0	0.0	0.0	0.0
12	0.07237562734545643	0.0	0.0	0.0	0.0
13	0.09549261876624401	0.0	0.0	0.0	0.0
14	0.12390275308244555	0.0	0.0	0.0	0.0
15	0.15134067747908128	0.0	0.0	0.0	0.0
16	0.1906611675592994	0.0	0.0	0.0	0.0
17	0.2226360715805757	0.0	0.0	0.0	0.0
18	0.25666341876537985	0.0	0.0	0.0	0.0
19	0.2878821595158827	0.0	0.0	0.0	0.0
20	0.3188848536175931	0.0	0.0	0.0	0.0
21	0.34427033485070097	0.0	0.0	0.0	0.0
22	0.3770014021427507	0.0	0.0	0.0	0.0
23	0.4152416589790067	0.0	0.0	0.0	0.0
24	0.45326586916647044	0.0	0.0	0.0	0.0
25	0.4852407731877467	0.0	0.0	0.0	0.0
26	0.5280180096486433	0.0	0.0	0.0	0.0
27	0.5611811702382778	0.0	0.0	0.0	0.0
28	0.613788529219229	0.0	0.0	0.0	0.0
29	0.6369055206400166	0.0	0.0	0.0	0.0
30	0.6680162380661232	0.0	0.0	0.0	0.0
31	0.6944819525431931	0.0	0.0	0.0	0.0
32	0.7279691831060162	0.0	0.0	0.0	0.0
33	0.7520583844463696	0.0	0.0	0.0	0.0
34	0.7778759589770623	0.0	0.0	0.0	0.0
35	0.827566688199316	0.0	0.0	0.0	0.0
36	0.8474429798882175	0.0	0.0	0.0	0.0
37	0.8729364844457215	0.0	0.0	0.0	0.0
38	0.8952973125957356	0.0	0.0	0.0	0.0
39	0.9165779075017878	0.0	0.0	0.0	0.0
40	0.9336455927563879	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACTA	20	6.3108135E-4	46.000004	34
TCGCAAG	20	6.3108135E-4	46.000004	29
CTCCGAA	20	6.3108135E-4	46.000004	27
ACGCATA	40	5.6097633E-9	46.000004	40
TAGGTGC	20	6.3108135E-4	46.000004	34
CGCATAA	40	5.6097633E-9	46.000004	41
CGCGCGA	20	6.3108135E-4	46.000004	15
GATTTCG	20	6.3108135E-4	46.000004	11
CACGACC	20	6.3108135E-4	46.000004	14
TCTAGTT	20	6.3108135E-4	46.000004	32
CGTAGTA	20	6.3108135E-4	46.000004	41
CTACGTA	20	6.3108135E-4	46.000004	31
AATTGCG	20	6.3108135E-4	46.000004	39
AGTTGCG	20	6.3108135E-4	46.000004	36
TCGCTTA	20	6.3108135E-4	46.000004	15
GTCGTAA	35	1.0192889E-7	46.000004	35
CATACGG	70	0.0	46.000004	36
GCGATAC	20	6.3108135E-4	46.000004	9
ACTCGCT	20	6.3108135E-4	46.000004	46
TCGTAAC	40	5.6097633E-9	46.000004	36
>>END_MODULE