FastQCFastQC Report
Fri 17 Jun 2016
SRR1527891_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527891_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695667
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC95690.5643207068368966No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG82830.4884803443128869No Hit
GTAATGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG65130.3840966416165438No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59540.3511302631943654No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC37030.21838014185568277No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC32710.1929034415365753No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGA26910.15869861240444028No Hit
GTAATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT24470.14430899463161104No Hit
GTAATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC23120.13634752578188997No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG22820.13457831048195193No Hit
GTAATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT21370.1260271031989182No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT20430.12048356192577907No Hit
GTAATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT19990.1178887128192033No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC18120.10686060411625631No Hit
GTAATGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC17770.10479651959966196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA207.8575406E-444.00000441
GTCGTAA207.8575406E-444.00000426
CGATAGT207.8575406E-444.00000428
GATCGTT302.5284317E-644.09
CGTGATA302.5284317E-644.039
TACGCGG254.4437387E-544.035
ATATACG302.5284317E-644.032
CGCGTTA551.8189894E-1244.022
AATAACG502.7284841E-1144.023
TAATGGG1719300.042.3147772
GTAATGG1752150.042.3061941
AATGGGG1565150.042.2992063
ATGGGGG638100.042.203734
ATGGGGA725250.042.1617364
AATGGGT54850.041.5934373
TGGGGAC113050.041.5090685
TGGGGAG200400.041.29945
TGGGGGG243450.041.2980085
TGGGGGA159250.041.2784965
ATGGGGC117650.041.1950724
Produced by FastQC (version 0.11.5)