##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527891_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1695667 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33709095005092 33.0 31.0 34.0 31.0 34.0 2 31.43237793741342 31.0 31.0 33.0 30.0 34.0 3 32.21503573520037 33.0 31.0 34.0 31.0 34.0 4 36.47406595752586 37.0 37.0 37.0 35.0 37.0 5 36.434563508047276 37.0 37.0 37.0 35.0 37.0 6 36.46231659871897 37.0 37.0 37.0 35.0 37.0 7 36.6775870498158 37.0 37.0 37.0 35.0 37.0 8 36.72297508885884 37.0 37.0 37.0 35.0 37.0 9 38.60103546274121 39.0 39.0 39.0 38.0 39.0 10 38.16320952168085 39.0 39.0 39.0 37.0 39.0 11 37.455926193055596 39.0 37.0 39.0 35.0 39.0 12 37.34011689795225 39.0 37.0 39.0 35.0 39.0 13 37.264172151725546 39.0 37.0 39.0 34.0 39.0 14 38.04842637145147 40.0 38.0 40.0 34.0 40.0 15 38.09991761354087 40.0 38.0 40.0 34.0 40.0 16 38.136718471256444 40.0 38.0 40.0 35.0 40.0 17 38.025992721448254 40.0 38.0 40.0 34.0 40.0 18 37.953814044856685 40.0 37.0 40.0 34.0 40.0 19 37.92369374411367 40.0 37.0 40.0 34.0 40.0 20 37.81429313656514 40.0 37.0 40.0 33.0 40.0 21 37.82698312817316 40.0 37.0 40.0 33.0 40.0 22 37.88762887996287 40.0 37.0 40.0 34.0 40.0 23 37.86517871728352 40.0 37.0 40.0 34.0 40.0 24 37.75531752401857 40.0 37.0 40.0 34.0 40.0 25 37.64868927684504 40.0 36.0 40.0 34.0 40.0 26 37.49081511877037 40.0 35.0 40.0 34.0 40.0 27 37.35332409016629 40.0 35.0 40.0 33.0 40.0 28 37.20335065788271 40.0 35.0 40.0 33.0 40.0 29 37.25280494342344 40.0 35.0 40.0 33.0 40.0 30 37.327341984009834 40.0 35.0 40.0 33.0 40.0 31 37.33104790032477 40.0 35.0 40.0 33.0 40.0 32 37.24969525266459 40.0 35.0 40.0 33.0 40.0 33 37.13269999357185 40.0 35.0 40.0 33.0 40.0 34 36.740023837227476 40.0 35.0 40.0 32.0 40.0 35 36.634540272353 40.0 35.0 40.0 31.0 40.0 36 36.59610348022342 40.0 35.0 40.0 32.0 40.0 37 36.41515698542226 40.0 35.0 40.0 31.0 40.0 38 36.2798792451584 40.0 35.0 40.0 31.0 40.0 39 36.13163197726912 40.0 35.0 40.0 31.0 40.0 40 35.9323369505923 40.0 35.0 40.0 30.0 40.0 41 35.80773819387887 39.0 35.0 40.0 30.0 40.0 42 35.71284515178983 39.0 35.0 40.0 29.0 40.0 43 35.62640424092702 39.0 35.0 40.0 29.0 40.0 44 35.39040448389925 39.0 35.0 40.0 27.0 40.0 45 35.35797771614356 39.0 35.0 40.0 27.0 40.0 46 35.22968306866856 39.0 35.0 40.0 26.0 40.0 47 35.16580967843333 38.0 35.0 40.0 26.0 40.0 48 35.1000650481492 38.0 35.0 40.0 26.0 40.0 49 35.00207352033153 38.0 35.0 40.0 26.0 40.0 50 34.43599598270179 37.0 34.0 40.0 24.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 2.0 13 2.0 14 10.0 15 28.0 16 47.0 17 113.0 18 262.0 19 526.0 20 1030.0 21 1689.0 22 2797.0 23 4554.0 24 7173.0 25 10321.0 26 13761.0 27 19365.0 28 25318.0 29 28424.0 30 29000.0 31 31223.0 32 38531.0 33 56879.0 34 136891.0 35 107527.0 36 111874.0 37 190200.0 38 553451.0 39 324668.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 99.2276195738904 0.527992819344836 0.18588555418015448 0.05850205258461714 8 98.09367051431678 1.5717118986216045 0.17851382376374605 0.1561037632978645 9 90.75101420267069 4.393787223552738 3.191723374931517 1.6634751988450565 10 39.21406738469287 46.72904526655293 6.444248782337569 7.612638566416637 11 31.134061109875937 26.682833362918544 25.359224423191584 16.82388110401394 12 30.12885195029449 21.92340831071195 25.206718064336926 22.74102167465664 13 24.979904662884874 21.843616700684745 27.788592925379806 25.38788571105058 14 24.2189061885382 24.472788584079304 28.363705845546328 22.944599381836177 15 25.891345411569606 24.897223334534434 26.463922456472883 22.74750879742308 16 29.37392778181093 26.07510790738983 23.762743510370846 20.788220800428387 17 27.868561456937 27.073358153458198 22.900840790084374 22.157239599520427 18 27.681968216636875 26.146761127037326 23.88305015076663 22.28822050555917 19 26.2911880693556 27.6725913755472 24.23064198336112 21.805578571736078 20 27.687806627126673 28.13931037167085 23.334770329315838 20.838112671886638 21 26.70205883584454 27.188357147954168 25.2977736784404 20.811810337760896 22 25.5699379654142 25.81998706113877 25.986765090079594 22.62330988336743 23 25.204418084447006 26.411612657438045 26.837875597036447 21.5460936610785 24 25.915111870432106 27.95130175913077 23.96077767627724 22.17280869415988 25 25.278489231671074 28.140489848537477 22.679099139158808 23.901921780632637 26 25.40457530871333 28.526119810080637 23.655352141664608 22.413952739541433 27 24.471019368779366 25.652796215294632 23.765279385634088 26.110905030291914 28 24.3501819637936 28.331624074774115 23.560817070804585 23.7573768906277 29 27.812123488868984 26.810570707574072 23.177428115308018 22.199877688248932 30 25.787964264209894 25.944362896724414 24.42006596814115 23.84760687092454 31 27.632784031298595 27.06592744919846 23.21894570101323 22.082342818489714 32 28.47050747581925 26.849375496486044 23.85698371201421 20.823133315680494 33 29.018020637306734 26.06732336007011 23.70895936525273 21.205696637370426 34 25.9999752309858 26.9381311306996 25.994608611242654 21.067285027071943 35 24.255469971403585 29.37286625263097 26.53923205440691 19.83243172155854 36 25.57424305597738 29.648627944047977 23.556335058711408 21.22079394126323 37 26.94768489331927 28.709115645937555 22.954565961359158 21.388633499384017 38 27.128262801599607 27.893448418822796 23.55202996814823 21.426258811429367 39 26.96118990344213 26.42252281845433 22.908861232777426 23.707426045326116 40 26.37976678203916 26.029344204964772 23.196712562077344 24.394176450918724 41 24.215839548684972 27.224449140072903 24.054958904077274 24.50475240716485 42 24.31013872417167 26.376169377595954 24.197321761878953 25.11637013635342 43 23.875855341880218 25.72674941483204 25.049080981112446 25.348314262175297 44 24.764119370135766 25.726572493302047 24.334141078407495 25.175167058154695 45 25.63186050091203 24.516134358927786 25.03286317419635 24.819141965963837 46 25.116606031726747 26.634946602133557 24.004772163402365 24.24367520273733 47 24.420301863514478 26.270724145719647 24.89197466247795 24.416999328287925 48 25.316291465246422 25.567402090150953 24.805283112780987 24.311023331821637 49 25.859027745424072 25.31281200848988 24.9990121881242 23.829148057961852 50 24.113225061288567 25.78224380140676 25.34772452374198 24.756806613562688 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 14.0 4 28.0 5 70.0 6 112.0 7 1090.0 8 2068.0 9 4906.0 10 7744.0 11 6224.5 12 4705.0 13 5469.5 14 6234.0 15 5916.0 16 5598.0 17 6378.5 18 7159.0 19 8064.0 20 8969.0 21 10111.5 22 11254.0 23 13323.5 24 15393.0 25 17882.0 26 20371.0 27 22654.5 28 24938.0 29 27360.5 30 29783.0 31 32448.5 32 35114.0 33 38558.0 34 42002.0 35 44214.5 36 46427.0 37 49421.0 38 52415.0 39 58354.5 40 64294.0 41 66416.5 42 68539.0 43 71241.0 44 73943.0 45 76928.5 46 79914.0 47 83219.0 48 86524.0 49 93374.5 50 100225.0 51 105411.5 52 110598.0 53 111390.0 54 112182.0 55 119628.0 56 127074.0 57 127068.5 58 127063.0 59 122151.5 60 117240.0 61 110183.5 62 103127.0 63 89353.5 64 75580.0 65 59081.5 66 42583.0 67 35849.0 68 29115.0 69 24923.0 70 20731.0 71 17354.0 72 13977.0 73 13384.5 74 12792.0 75 9130.5 76 5469.0 77 4043.5 78 2618.0 79 1892.5 80 1167.0 81 815.5 82 464.0 83 289.0 84 114.0 85 66.0 86 18.0 87 9.0 88 0.0 89 0.5 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1695667.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.101013308143214 #Duplication Level Percentage of deduplicated Percentage of total 1 70.56813992645479 8.539460003809419 2 9.00933275730302 2.1804411118722893 3 3.4593604997225644 1.2558530233442313 4 1.6369982879732963 0.7923735227269005 5 0.950787318228003 0.575274499554543 6 0.6048373150451498 0.43914866391737806 7 0.47785598707578275 0.4047779161285972 8 0.3877512367614233 0.3753746301039179 9 0.3392210582523607 0.36944266862828173 >10 8.335885416593184 28.463545673037288 >50 2.966676782705391 24.366364103770827 >100 1.2160947572893341 24.957880515687354 >500 0.031207319637160096 2.5399599652494356 >1k 0.013869919838737822 2.922695300914072 >5k 0.001981417119819689 1.8174084012554608 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 9569 0.5643207068368966 No Hit GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 8283 0.4884803443128869 No Hit GTAATGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG 6513 0.3840966416165438 No Hit GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5954 0.3511302631943654 No Hit GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC 3703 0.21838014185568277 No Hit GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC 3271 0.1929034415365753 No Hit GTAATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGA 2691 0.15869861240444028 No Hit GTAATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT 2447 0.14430899463161104 No Hit GTAATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC 2312 0.13634752578188997 No Hit GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG 2282 0.13457831048195193 No Hit GTAATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 2137 0.1260271031989182 No Hit GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT 2043 0.12048356192577907 No Hit GTAATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT 1999 0.1178887128192033 No Hit GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC 1812 0.10686060411625631 No Hit GTAATGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC 1777 0.10479651959966196 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.12166303879240441 0.0 0.0 0.0 0.0 8 0.16447804905090446 0.0 0.0 0.0 0.0 9 0.22398265697215314 0.0 0.0 0.0 0.0 10 0.32636124899523317 0.0 0.0 0.0 0.0 11 0.37589927739349766 0.0 0.0 0.0 0.0 12 0.411283583392258 0.0 0.0 0.0 0.0 13 0.43269108852150806 0.0 0.0 0.0 0.0 14 0.4670728391836369 0.0 0.0 0.0 0.0 15 0.5022802236524034 0.0 0.0 0.0 0.0 16 0.5400234833844145 0.0 0.0 0.0 0.0 17 0.5755257370698375 0.0 0.0 0.0 0.0 18 0.6130920752718547 0.0 0.0 0.0 0.0 19 0.6644582928133885 0.0 0.0 0.0 0.0 20 0.7049733231819691 0.0 0.0 0.0 0.0 21 0.7492626795237508 0.0 0.0 0.0 0.0 22 0.7989776294520091 0.0 0.0 0.0 0.0 23 0.8531156176301126 0.0 0.0 0.0 0.0 24 0.9084330826748412 0.0 0.0 0.0 0.0 25 0.9608018555530066 0.0 0.0 0.0 0.0 26 1.0144090791411284 0.0 0.0 0.0 0.0 27 1.0585215139529165 0.0 0.0 0.0 0.0 28 1.1069980131712183 0.0 0.0 0.0 0.0 29 1.1410259207733593 0.0 0.0 0.0 0.0 30 1.1778845728554015 0.0 0.0 0.0 0.0 31 1.2038920377644904 0.0 0.0 0.0 0.0 32 1.2290738688669414 0.0 0.0 0.0 0.0 33 1.2520146939228045 0.0 0.0 0.0 0.0 34 1.277255498868587 0.0 0.0 0.0 0.0 35 1.3109295634107405 0.0 0.0 0.0 0.0 36 1.3319832254800028 0.0 0.0 0.0 0.0 37 1.3545112336325469 0.0 0.0 0.0 0.0 38 1.3750931049551591 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGCGA 20 7.8575406E-4 44.000004 41 GTCGTAA 20 7.8575406E-4 44.000004 26 CGATAGT 20 7.8575406E-4 44.000004 28 GATCGTT 30 2.5284317E-6 44.0 9 CGTGATA 30 2.5284317E-6 44.0 39 TACGCGG 25 4.4437387E-5 44.0 35 ATATACG 30 2.5284317E-6 44.0 32 CGCGTTA 55 1.8189894E-12 44.0 22 AATAACG 50 2.7284841E-11 44.0 23 TAATGGG 171930 0.0 42.314777 2 GTAATGG 175215 0.0 42.306194 1 AATGGGG 156515 0.0 42.299206 3 ATGGGGG 63810 0.0 42.20373 4 ATGGGGA 72525 0.0 42.161736 4 AATGGGT 5485 0.0 41.593437 3 TGGGGAC 11305 0.0 41.509068 5 TGGGGAG 20040 0.0 41.2994 5 TGGGGGG 24345 0.0 41.298008 5 TGGGGGA 15925 0.0 41.278496 5 ATGGGGC 11765 0.0 41.195072 4 >>END_MODULE