FastQCFastQC Report
Fri 17 Jun 2016
SRR1527890_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527890_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2529976
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC141530.5594124213036014No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT117550.464628913475859No Hit
GTAATGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC100540.39739507410346975No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80930.3198844574019674No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA57100.22569384057398173No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC48660.1923338403210149No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGAAG36410.14391440867423247No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT34110.13482341334463252No Hit
GTAATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT33630.13292616214541167No Hit
GTAATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG32470.12834113841396125No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC30790.12170075921668821No Hit
GTAATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC28940.1143884368863578No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC28020.11075203875451783No Hit
GTAATGGGGAACAGACACTGTTGACTTTGCAACTAAGTTATGACCTGGTACG26880.10624606715636828No Hit
GTAATGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT26380.10426976382384655No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGC206.3128996E-446.014
ATCGTAC206.3128996E-446.025
CATAACG301.8622959E-646.044
TTCGCGA206.3128996E-446.017
AATCGGT206.3128996E-446.040
CTAGGCG453.110472E-1045.99999623
AATGGGG2337100.044.1173673
GTAATGG2616700.044.113731
TAATGGG2570950.044.104322
ATGGGGA1092150.044.0456924
ATGGGGG939650.043.9781844
TATCGTA850.043.29411735
TGGGGGG357400.043.2327965
TGGGGAG295000.043.0918665
TGGGGAC181500.042.882645
ATGGGGT133200.042.8746224
ATGGGGC185850.042.8689774
TGGGGAT367950.042.7933165
TGGGGGA243050.042.7541665
AATCCCG700.042.71428336
Produced by FastQC (version 0.11.5)