##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527890_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2529976
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.027063497835556	33.0	31.0	33.0	31.0	34.0
2	31.16039598794613	31.0	31.0	33.0	30.0	33.0
3	31.91989647332623	31.0	31.0	34.0	30.0	34.0
4	36.323807419517024	37.0	37.0	37.0	35.0	37.0
5	36.18574010188239	37.0	37.0	37.0	35.0	37.0
6	36.24622921324155	37.0	37.0	37.0	35.0	37.0
7	36.61809598193817	37.0	37.0	37.0	35.0	37.0
8	36.66368613773411	37.0	37.0	37.0	35.0	37.0
9	38.52348204093636	39.0	39.0	39.0	37.0	39.0
10	37.929695380509536	39.0	38.0	39.0	35.0	39.0
11	37.15926079931193	39.0	35.0	39.0	34.0	39.0
12	37.03881183062606	39.0	37.0	39.0	33.0	39.0
13	36.960972752310695	39.0	37.0	39.0	33.0	39.0
14	37.77120454897596	40.0	37.0	40.0	33.0	40.0
15	37.838460522945674	40.0	37.0	40.0	33.0	40.0
16	37.87315057534143	40.0	37.0	40.0	34.0	40.0
17	37.784247755709934	40.0	37.0	40.0	33.0	40.0
18	37.7573799119043	40.0	37.0	40.0	33.0	40.0
19	37.74012559802939	40.0	37.0	40.0	34.0	40.0
20	37.726280407403074	40.0	37.0	40.0	33.0	40.0
21	37.7100604906924	40.0	36.0	40.0	33.0	40.0
22	37.72146217987839	40.0	36.0	40.0	33.0	40.0
23	37.71136485089187	40.0	36.0	40.0	34.0	40.0
24	37.60127408323241	40.0	36.0	40.0	34.0	40.0
25	37.49307779994751	40.0	36.0	40.0	34.0	40.0
26	37.30376849424658	40.0	35.0	40.0	33.0	40.0
27	37.17524751222936	40.0	35.0	40.0	33.0	40.0
28	37.06418519385164	39.0	35.0	40.0	33.0	40.0
29	37.00050593365312	39.0	35.0	40.0	33.0	40.0
30	36.815013264947964	39.0	35.0	40.0	32.0	40.0
31	36.70556440061092	39.0	35.0	40.0	32.0	40.0
32	36.53875530835075	39.0	35.0	40.0	31.0	40.0
33	36.425755817446486	39.0	35.0	40.0	31.0	40.0
34	36.17495976246415	39.0	35.0	40.0	30.0	40.0
35	35.98025752022944	39.0	35.0	40.0	30.0	40.0
36	35.97603929839651	38.0	35.0	40.0	30.0	40.0
37	35.784786891259046	38.0	35.0	40.0	30.0	40.0
38	35.6571311348408	38.0	35.0	40.0	29.0	40.0
39	35.51839661720111	38.0	35.0	40.0	29.0	40.0
40	35.30031154445734	38.0	35.0	40.0	27.0	40.0
41	35.159065145282014	38.0	34.0	40.0	27.0	40.0
42	35.05697010564527	38.0	34.0	40.0	27.0	40.0
43	34.95786718925397	38.0	34.0	40.0	26.0	40.0
44	34.87140668528081	38.0	34.0	40.0	26.0	40.0
45	34.75012134502462	38.0	34.0	40.0	25.0	40.0
46	34.61060500178658	37.0	34.0	40.0	24.0	40.0
47	34.470242405461555	37.0	34.0	40.0	24.0	40.0
48	34.32388923847499	37.0	33.0	40.0	23.0	40.0
49	34.593192978905726	37.0	34.0	40.0	25.0	40.0
50	34.61925132886636	37.0	34.0	40.0	25.0	40.0
51	34.60183258655418	37.0	34.0	40.0	25.0	40.0
52	34.149994308246406	36.0	34.0	40.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	10.0
15	30.0
16	92.0
17	205.0
18	505.0
19	1102.0
20	1977.0
21	3691.0
22	6025.0
23	9990.0
24	14177.0
25	19780.0
26	27152.0
27	36101.0
28	41437.0
29	43272.0
30	46485.0
31	56925.0
32	74752.0
33	107982.0
34	220249.0
35	170573.0
36	210787.0
37	329063.0
38	793721.0
39	313890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.27940027889592	0.5124949801895354	0.1579856884017872	0.05011905251275111
8	98.07974462998858	1.5964183059444044	0.16877630459735588	0.15506075946965506
9	90.75161187299801	4.351938516412804	3.208172725749177	1.688276884840014
10	39.15882996518544	46.863408980954766	6.330771517200164	7.646989536659636
11	31.1886357815252	26.3913965982286	25.382572799109553	17.037394821136644
12	30.241709802780736	21.60613381312708	25.300872419343108	22.851283964749072
13	25.066285213772776	21.567358741743003	27.756785044601212	25.609570999883
14	24.250190515641254	24.25766094223819	28.437582016588298	23.05456652553226
15	26.017480007715488	24.682210424130506	26.49677309191866	22.803536476235347
16	29.473560223496193	25.769216783084108	23.814336578686913	20.94288641473279
17	27.97821006997695	26.788633568065467	23.02017884754638	22.212977514411204
18	27.86556078002321	25.758900479688347	23.971334115422437	22.404204624866004
19	26.3910013375621	27.391880397284403	24.34141667747046	21.875701587683043
20	27.893110448478563	27.90164807887506	23.335913067950052	20.86932840469633
21	26.85460257330504	26.914879824946958	25.396920761303665	20.833596840444336
22	25.744117730761083	25.50391782372639	26.015780386849517	22.736184058663007
23	25.336959718194958	26.147797449461972	26.82606475318343	21.689178079159642
24	26.010918680651518	27.67125063636967	23.987935063415623	22.32989561956319
25	25.35118910218911	27.85429585102784	22.71345657033901	24.081058476444046
26	25.50312730239338	28.227066185608084	23.69069904220435	22.57910746979418
27	24.520469759396928	25.396367396370557	23.837103593077565	26.246059251154954
28	24.385883502452195	28.191374147422742	23.56887970478771	23.853862645337347
29	27.86556078002321	26.62254503600034	23.306466148295478	22.20542803568097
30	25.917281428756638	25.764315550819454	24.374460469190222	23.94394255123369
31	27.74986798293739	26.8191476915196	23.287296005970017	22.143688319572995
32	28.654619648565838	26.53811735763501	23.980741319285244	20.82652167451391
33	29.21415064806939	25.82913830012617	23.688367004271978	21.268344047532466
34	26.0001675905226	26.71155773809712	26.06048436823116	21.22779030314912
35	24.431496583366798	29.17015813588745	26.51400645697825	19.884338823767497
36	25.585222942826334	29.514193020012836	23.606271363839024	21.2943126733218
37	26.951520488731912	28.68201121275459	23.008004819018048	21.358463479495455
38	27.15448684098189	27.879908742217317	23.549156197529147	21.416448219271643
39	26.97899110505396	26.30060522313255	22.94306349151138	23.777340180302104
40	26.35376778277739	26.05404161936714	23.161682166154936	24.430508431700538
41	24.231178477582397	27.134249494856867	24.086117812975303	24.548454214585437
42	24.28070463909539	26.21151346890247	24.271574117699142	25.236207774302997
43	23.874218569662318	25.6411523271367	25.065020379639964	25.419608723561016
44	24.65272397840928	25.586962089758952	24.376832033189245	25.38348189864252
45	25.659215739595947	24.334697246139882	25.08901270209678	24.91707431216739
46	25.04367630364873	26.475784750527275	24.149043311082792	24.3314956347412
47	24.615371845424622	26.049733278102245	24.913912226835354	24.420982649637782
48	25.535933937713246	25.322414125667596	24.761302083498023	24.380349853121135
49	25.95838853807309	25.149131849472088	25.016600947993183	23.875878664461638
50	24.147106533816924	25.663286924460945	25.36925251464836	24.820354027073773
51	22.821797519027847	26.12933877633622	25.789137920675927	25.259725783960008
52	23.87868501519382	25.66202209032813	25.769098204884155	24.690194689593895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	17.0
4	34.0
5	99.5
6	165.0
7	1422.0
8	2679.0
9	6604.0
10	10529.0
11	8466.5
12	6404.0
13	7384.0
14	7750.5
15	7137.0
16	8044.5
17	8952.0
18	9747.0
19	10542.0
20	11267.0
21	11992.0
22	14356.5
23	16721.0
24	20296.5
25	23872.0
26	27274.5
27	30677.0
28	34156.5
29	37636.0
30	42632.5
31	47629.0
32	51530.5
33	55432.0
34	57280.0
35	59128.0
36	64978.5
37	70829.0
38	77482.0
39	88264.5
40	92394.0
41	96111.0
42	99828.0
43	106605.0
44	113382.0
45	112836.5
46	112291.0
47	120245.0
48	128199.0
49	132836.0
50	137473.0
51	147210.0
52	156947.0
53	161792.5
54	166638.0
55	176144.5
56	185651.0
57	184325.0
58	182999.0
59	177676.5
60	172354.0
61	164005.0
62	155656.0
63	129475.0
64	94095.0
65	84896.0
66	66308.0
67	47720.0
68	39643.0
69	31566.0
70	29134.0
71	26702.0
72	23452.0
73	20202.0
74	15279.5
75	10357.0
76	7746.0
77	5135.0
78	3662.0
79	2189.0
80	1603.5
81	1018.0
82	587.0
83	156.0
84	95.5
85	35.0
86	29.0
87	23.0
88	17.5
89	6.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2529976.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.875690415470272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.34761985256641	8.829293164961502
2	8.643988722763952	2.0530666805272197
3	3.4457368948493965	1.2276151384918583
4	1.7696086818429206	0.840612994483798
5	1.0138341502174888	0.601999025030714
6	0.6336256573780883	0.45148452877926076
7	0.39749257307929314	0.33043491182368645
8	0.318404854491157	0.3025021982975871
9	0.2486901787723027	0.26580308152210413
>10	4.307091950433661	14.406510805379177
>50	3.1347687063127725	26.44042836719046
>100	1.6650541623288568	34.35189505157702
>500	0.05481523080280499	4.245775744173392
>1k	0.017607316560900997	3.689217222535974
>5k	6.644270400339999E-4	0.5445649162941294
>10k+	9.96640560051E-4	1.4187961689320787
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	14153	0.5594124213036014	No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	11755	0.464628913475859	No Hit
GTAATGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	10054	0.39739507410346975	No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8093	0.3198844574019674	No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	5710	0.22569384057398173	No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	4866	0.1923338403210149	No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGAAG	3641	0.14391440867423247	No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3411	0.13482341334463252	No Hit
GTAATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3363	0.13292616214541167	No Hit
GTAATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3247	0.12834113841396125	No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	3079	0.12170075921668821	No Hit
GTAATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2894	0.1143884368863578	No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	2802	0.11075203875451783	No Hit
GTAATGGGGAACAGACACTGTTGACTTTGCAACTAAGTTATGACCTGGTACG	2688	0.10624606715636828	No Hit
GTAATGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	2638	0.10426976382384655	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.11320265488684478	0.0	0.0	0.0	0.0
8	0.1522544087374742	0.0	0.0	0.0	0.0
9	0.20680038071507398	0.0	0.0	0.0	0.0
10	0.29431109227913627	0.0	0.0	0.0	0.0
11	0.343125784592423	0.0	0.0	0.0	0.0
12	0.3739165905131116	0.0	0.0	0.0	0.0
13	0.392138107238962	0.0	0.0	0.0	0.0
14	0.4220988657599914	0.0	0.0	0.0	0.0
15	0.45569602241286083	0.0	0.0	0.0	0.0
16	0.49190189946465895	0.0	0.0	0.0	0.0
17	0.5237203831182589	0.0	0.0	0.0	0.0
18	0.557119909437876	0.0	0.0	0.0	0.0
19	0.5973179192213681	0.0	0.0	0.0	0.0
20	0.6358558342055419	0.0	0.0	0.0	0.0
21	0.6751447444560739	0.0	0.0	0.0	0.0
22	0.7132083466404424	0.0	0.0	0.0	0.0
23	0.7573984891556284	0.0	0.0	0.0	0.0
24	0.801114318871009	0.0	0.0	0.0	0.0
25	0.8386245561222715	0.0	0.0	0.0	0.0
26	0.8782692009726575	0.0	0.0	0.0	0.0
27	0.9109967841592174	0.0	0.0	0.0	0.0
28	0.9483093910772276	0.0	0.0	0.0	0.0
29	0.9726574481338953	0.0	0.0	0.0	0.0
30	1.0017091071219648	0.0	0.0	0.0	0.0
31	1.0214326143805317	0.0	0.0	0.0	0.0
32	1.0416304344389038	0.0	0.0	0.0	0.0
33	1.0596147947648515	0.0	0.0	0.0	0.0
34	1.079298775956768	0.0	0.0	0.0	0.0
35	1.1099314776108549	0.0	0.0	0.0	0.0
36	1.1298921412693244	0.0	0.0	0.0	0.0
37	1.1506433262608025	0.0	0.0	0.0	0.0
38	1.168509108386799	0.0	0.0	0.0	0.0
39	1.1875606725123085	0.0	0.0	0.0	0.0
40	1.2059007674381101	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGC	20	6.3128996E-4	46.0	14
ATCGTAC	20	6.3128996E-4	46.0	25
CATAACG	30	1.8622959E-6	46.0	44
TTCGCGA	20	6.3128996E-4	46.0	17
AATCGGT	20	6.3128996E-4	46.0	40
CTAGGCG	45	3.110472E-10	45.999996	23
AATGGGG	233710	0.0	44.117367	3
GTAATGG	261670	0.0	44.11373	1
TAATGGG	257095	0.0	44.10432	2
ATGGGGA	109215	0.0	44.045692	4
ATGGGGG	93965	0.0	43.978184	4
TATCGTA	85	0.0	43.294117	35
TGGGGGG	35740	0.0	43.232796	5
TGGGGAG	29500	0.0	43.091866	5
TGGGGAC	18150	0.0	42.88264	5
ATGGGGT	13320	0.0	42.874622	4
ATGGGGC	18585	0.0	42.868977	4
TGGGGAT	36795	0.0	42.793316	5
TGGGGGA	24305	0.0	42.754166	5
AATCCCG	70	0.0	42.714283	36
>>END_MODULE