FastQCFastQC Report
Fri 17 Jun 2016
SRR1527889_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527889_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2561099
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC137990.5387921357198608No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT113020.4412949284662561No Hit
GTAATGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC94880.37046596012102617No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75140.2933896737299105No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA55880.2181875827525605No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC46330.18089890316617985No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGAAG34710.13552775585793442No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT33270.12990516961663723No Hit
GTAATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT32030.12506349813107576No Hit
GTAATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG31040.12119797009018393No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC29260.11424782876413601No Hit
GTAATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC27780.10846905957169169No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC26990.10538444628653558No Hit
GTAATGGGGAACAGACACTGTTGACTTTGCAACTAAGTTATGACCTGGTACG26320.10276838185482091No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG206.312914E-446.038
CGTAACA206.312914E-446.029
AATCGCG253.418339E-546.019
AATGGGG2364500.044.1508563
TAATGGG2597800.044.133652
GTAATGG2648600.044.1242941
ATGGGGG958200.044.0221254
ATGGGGA1098800.044.0114674
TGGGGGG355450.043.204675
TGGGGAG303400.043.1117365
TGGGGAC183000.043.0715835
ATGGGGT141450.043.0569084
ATGGGGC181050.042.8748974
TGGGGAT362050.042.7347075
TACGTTA700.042.71428728
TGGGGGA247750.042.6764875
AATGGGT85400.042.633493
TGGGGGC188850.042.4924555
TGGGGGT195800.042.0178765
ATGGGTA44700.041.935124
Produced by FastQC (version 0.11.5)