##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527889_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2561099
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97035881861654	33.0	31.0	33.0	31.0	34.0
2	31.132188954819785	31.0	31.0	33.0	30.0	33.0
3	31.872962349366425	31.0	31.0	34.0	30.0	34.0
4	36.298175900267815	37.0	37.0	37.0	35.0	37.0
5	36.189096946271896	37.0	37.0	37.0	35.0	37.0
6	36.15226627318975	37.0	37.0	37.0	35.0	37.0
7	36.574750527019845	37.0	37.0	37.0	35.0	37.0
8	36.63684886839595	37.0	37.0	37.0	35.0	37.0
9	38.48172874223136	39.0	39.0	39.0	37.0	39.0
10	37.84519770614099	39.0	38.0	39.0	35.0	39.0
11	37.12263368186861	39.0	35.0	39.0	34.0	39.0
12	36.987980160079715	39.0	37.0	39.0	33.0	39.0
13	36.75482907923512	39.0	35.0	39.0	33.0	39.0
14	37.7013774164919	40.0	37.0	40.0	33.0	40.0
15	37.78956963397354	40.0	37.0	40.0	33.0	40.0
16	37.85805195347778	40.0	37.0	40.0	34.0	40.0
17	37.801153723460125	40.0	37.0	40.0	33.0	40.0
18	37.78529529705802	40.0	37.0	40.0	33.0	40.0
19	37.72119859482199	40.0	37.0	40.0	33.0	40.0
20	37.720272820379066	40.0	36.0	40.0	33.0	40.0
21	37.7596211626337	40.0	37.0	40.0	33.0	40.0
22	37.66059765749001	40.0	36.0	40.0	33.0	40.0
23	37.6823188014208	40.0	36.0	40.0	34.0	40.0
24	37.616595453748566	40.0	36.0	40.0	34.0	40.0
25	37.47915640902597	40.0	36.0	40.0	33.0	40.0
26	37.24612207493736	40.0	35.0	40.0	33.0	40.0
27	37.11922264621555	39.0	35.0	40.0	33.0	40.0
28	37.02762876405793	39.0	35.0	40.0	33.0	40.0
29	36.964270416723444	39.0	35.0	40.0	33.0	40.0
30	36.763991161606796	39.0	35.0	40.0	32.0	40.0
31	36.67736897324156	39.0	35.0	40.0	32.0	40.0
32	36.512900906993444	39.0	35.0	40.0	31.0	40.0
33	36.242723143463024	39.0	35.0	40.0	31.0	40.0
34	36.088724801345045	39.0	35.0	40.0	30.0	40.0
35	35.959542758792225	38.0	35.0	40.0	30.0	40.0
36	35.82547531352751	38.0	35.0	40.0	30.0	40.0
37	35.70745020008988	38.0	35.0	40.0	29.0	40.0
38	35.32183293187807	38.0	35.0	40.0	27.0	40.0
39	35.346726932461415	38.0	34.0	40.0	27.0	40.0
40	35.20730045968547	38.0	34.0	40.0	27.0	40.0
41	34.87365541121214	38.0	34.0	40.0	25.0	40.0
42	34.907668543855586	38.0	34.0	40.0	26.0	40.0
43	34.92524498272031	38.0	34.0	40.0	26.0	40.0
44	34.839162406451294	38.0	34.0	40.0	26.0	40.0
45	34.71356632445681	38.0	34.0	40.0	25.0	40.0
46	34.40749928058228	37.0	34.0	40.0	23.0	40.0
47	34.18442941877686	37.0	33.0	40.0	23.0	40.0
48	34.19906610404362	37.0	33.0	40.0	23.0	40.0
49	34.150548260727135	36.0	33.0	40.0	24.0	40.0
50	33.98282065628857	36.0	33.0	40.0	23.0	40.0
51	34.24228934531621	36.0	34.0	40.0	24.0	40.0
52	33.971509496509114	36.0	33.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	9.0
15	30.0
16	94.0
17	239.0
18	519.0
19	1118.0
20	2133.0
21	3824.0
22	6296.0
23	10349.0
24	15011.0
25	20394.0
26	28350.0
27	37063.0
28	42758.0
29	44384.0
30	48434.0
31	60292.0
32	79801.0
33	118264.0
34	222627.0
35	173964.0
36	225815.0
37	360378.0
38	792141.0
39	266809.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.28714196522664	0.5097811525442788	0.15540203639140854	0.047674845837665784
8	98.07816878613438	1.6043893656590393	0.16543679100261255	0.15200505720395815
9	90.71917953972103	4.358363343236634	3.2116681159143003	1.7107890011280313
10	39.2589665608397	46.72646391256254	6.389522622905244	7.625046903692516
11	31.246742121253412	26.409678032750783	25.360558104157626	16.983021741838172
12	30.307145487152194	21.592253950354905	25.281060982023735	22.81953958046917
13	25.29378989254222	21.54664852861994	27.760387240009077	25.39917433882876
14	24.241897716566207	24.219329280125447	28.469731158381617	23.06904184492673
15	26.10129479571075	24.6115827619315	26.503075437536776	22.784047004820977
16	29.505692673340622	25.72708044476219	23.884160666963673	20.88306621493351
17	28.039798539611315	26.75339766248786	23.02347546892955	22.183328328971275
18	27.864678405637576	25.727158536237766	23.987553780623085	22.420609277501573
19	26.408858072257264	27.355053436044447	24.353880892538708	21.882207599159578
20	27.902084222437324	27.835862651150933	23.33603659991277	20.926016526498977
21	26.84039156627682	26.878148794716644	25.43259749037425	20.848862148632286
22	25.95987113344701	25.424983571505827	25.97213149511206	22.643013799935105
23	25.382150397153723	26.0328866631083	26.924339902518412	21.66062303721957
24	26.056821700371597	27.66218720947531	23.990677439646028	22.29031365050707
25	25.433925045459	27.77846541660436	22.663668995224313	24.123940542712326
26	25.574294472802496	28.13464063669542	23.726845389420713	22.56421950108137
27	24.57339603037602	25.376254490747918	23.799743781868642	26.25060569700742
28	24.417993993984613	28.212575929317847	23.529273956219576	23.840156120477968
29	27.920474764934898	26.58171355343936	23.27594520945891	22.22186647216683
30	25.930157326991264	25.70388727651684	24.441499528132262	23.924455868359637
31	27.835667422461995	26.760777306929562	23.262982024513697	22.140573246094743
32	28.698109678696525	26.536381451868905	23.89868568142036	20.86682318801421
33	29.282155824511275	25.716108592444105	23.717123000711805	21.28461258233282
34	26.0319886111392	26.659922166226295	26.111173367370803	21.196915855263697
35	24.44852776093388	29.155257176704218	26.52076315675419	19.87545190560771
36	25.87276009244469	29.41292000035922	23.558675396773026	21.155644510423063
37	27.005281716950417	28.632278564788006	23.024295429423074	21.338144288838503
38	27.1663844310587	27.78486891760139	23.51670122865223	21.53204542268768
39	27.03624498701534	26.272588447381377	22.890641868978904	23.80052469662438
40	26.358879527890178	26.008092619613688	23.189966494852403	24.44306135764373
41	24.552311331971158	27.0635379577283	23.975527693384755	24.408623016915783
42	24.272939078106702	26.231434239754105	24.27356380991129	25.2220628722279
43	23.928165213449383	25.636767653261355	25.044639039724743	25.39042809356452
44	24.711696033616818	25.56769574311653	24.344509915469885	25.376098307796767
45	25.67530579645691	24.302574793086873	25.03097303149937	24.991146378956845
46	25.21706501779119	26.41186459406684	24.137957962577783	24.233112425564183
47	24.91004838157369	25.989897305805048	24.83707189765019	24.262982414971074
48	25.524159745484265	25.399525750468843	24.772490247350845	24.303824256696053
49	26.06349852153314	25.058226956474545	24.992825345681677	23.88544917631064
50	24.205468043211138	25.657071436910485	25.32108286325519	24.816377656623192
51	22.89782628473167	26.089581074374713	25.847224179932134	25.165368460961485
52	23.896225799939792	25.567227194263086	25.763783438281767	24.77276356751535
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	22.0
4	44.0
5	102.5
6	161.0
7	1444.0
8	2727.0
9	6401.5
10	10076.0
11	8408.5
12	6741.0
13	7540.5
14	7843.0
15	7346.0
16	8141.5
17	8937.0
18	9746.5
19	10556.0
20	11285.5
21	12015.0
22	14301.5
23	16588.0
24	19956.5
25	23325.0
26	27091.0
27	30857.0
28	34424.0
29	37991.0
30	42923.5
31	47856.0
32	51980.5
33	56105.0
34	57954.0
35	59803.0
36	65707.0
37	71611.0
38	78327.5
39	89036.5
40	93029.0
41	96953.0
42	100877.0
43	108052.0
44	115227.0
45	114607.5
46	113988.0
47	121984.5
48	129981.0
49	134838.0
50	139695.0
51	149329.5
52	158964.0
53	163740.5
54	168517.0
55	178571.5
56	188626.0
57	187076.5
58	185527.0
59	179656.0
60	173785.0
61	165886.0
62	157987.0
63	131497.5
64	95316.5
65	85625.0
66	67068.0
67	48511.0
68	40339.5
69	32168.0
70	29841.5
71	27515.0
72	24084.5
73	20654.0
74	15560.5
75	10467.0
76	7866.5
77	5266.0
78	3742.0
79	2218.0
80	1639.0
81	1060.0
82	629.0
83	198.0
84	128.5
85	59.0
86	38.0
87	17.0
88	10.0
89	2.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2561099.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.421096085496595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.31242430127867	9.230417625923746
2	8.680630500051102	2.156458910476542
3	3.5814764718008303	1.3345759015255034
4	1.8017876461953586	0.8952070991621317
5	1.031664866486097	0.6407204217327412
6	0.6013237471522391	0.4481460025121293
7	0.43135722553563655	0.3750550681885957
8	0.28659956554205457	0.2847904593327551
9	0.24948738471518117	0.27890160998998675
>10	4.404718962213443	15.53733225740175
>50	3.0406147156947947	26.695808991310415
>100	1.5135769257512803	32.85108125559132
>500	0.04792501217935206	3.985529826039823
>1k	0.014771407863498922	3.3414845152252437
>5k	9.847605242332613E-4	0.921054923480712
>10k+	6.565070161555076E-4	1.0234351321066557
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	13799	0.5387921357198608	No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	11302	0.4412949284662561	No Hit
GTAATGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	9488	0.37046596012102617	No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7514	0.2933896737299105	No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	5588	0.2181875827525605	No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	4633	0.18089890316617985	No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGAAG	3471	0.13552775585793442	No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3327	0.12990516961663723	No Hit
GTAATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3203	0.12506349813107576	No Hit
GTAATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3104	0.12119797009018393	No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	2926	0.11424782876413601	No Hit
GTAATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2778	0.10846905957169169	No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	2699	0.10538444628653558	No Hit
GTAATGGGGAACAGACACTGTTGACTTTGCAACTAAGTTATGACCTGGTACG	2632	0.10276838185482091	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.10967947744308205	0.0	0.0	0.0	0.0
8	0.14724147719397024	0.0	0.0	0.0	0.0
9	0.20237405894891217	0.0	0.0	0.0	0.0
10	0.2887432309332829	0.0	0.0	0.0	0.0
11	0.3343096069304623	0.0	0.0	0.0	0.0
12	0.36702993519578897	0.0	0.0	0.0	0.0
13	0.384912883102137	0.0	0.0	0.0	0.0
14	0.41447050660673407	0.0	0.0	0.0	0.0
15	0.44590232552509684	0.0	0.0	0.0	0.0
16	0.47979402592402715	0.0	0.0	0.0	0.0
17	0.512865765829435	0.0	0.0	0.0	0.0
18	0.5455080026191881	0.0	0.0	0.0	0.0
19	0.5881849940201452	0.0	0.0	0.0	0.0
20	0.6262936341000485	0.0	0.0	0.0	0.0
21	0.6642460912288045	0.0	0.0	0.0	0.0
22	0.7050879329537827	0.0	0.0	0.0	0.0
23	0.7463592777944156	0.0	0.0	0.0	0.0
24	0.7896219552621746	0.0	0.0	0.0	0.0
25	0.8281600984577324	0.0	0.0	0.0	0.0
26	0.869119077396071	0.0	0.0	0.0	0.0
27	0.9013708568079563	0.0	0.0	0.0	0.0
28	0.9415489209905591	0.0	0.0	0.0	0.0
29	0.9656401412050062	0.0	0.0	0.0	0.0
30	0.9919960142110867	0.0	0.0	0.0	0.0
31	1.0113627001533325	0.0	0.0	0.0	0.0
32	1.0324083528204102	0.0	0.0	0.0	0.0
33	1.0507988953179865	0.0	0.0	0.0	0.0
34	1.0710636332293284	0.0	0.0	0.0	0.0
35	1.101987857556463	0.0	0.0	0.0	0.0
36	1.1219792753032976	0.0	0.0	0.0	0.0
37	1.1407212294409548	0.0	0.0	0.0	0.0
38	1.1585260858717292	0.0	0.0	0.0	0.0
39	1.1747300670532455	0.0	0.0	0.0	0.0
40	1.1940186615199178	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	20	6.312914E-4	46.0	38
CGTAACA	20	6.312914E-4	46.0	29
AATCGCG	25	3.418339E-5	46.0	19
AATGGGG	236450	0.0	44.150856	3
TAATGGG	259780	0.0	44.13365	2
GTAATGG	264860	0.0	44.124294	1
ATGGGGG	95820	0.0	44.022125	4
ATGGGGA	109880	0.0	44.011467	4
TGGGGGG	35545	0.0	43.20467	5
TGGGGAG	30340	0.0	43.111736	5
TGGGGAC	18300	0.0	43.071583	5
ATGGGGT	14145	0.0	43.056908	4
ATGGGGC	18105	0.0	42.874897	4
TGGGGAT	36205	0.0	42.734707	5
TACGTTA	70	0.0	42.714287	28
TGGGGGA	24775	0.0	42.676487	5
AATGGGT	8540	0.0	42.63349	3
TGGGGGC	18885	0.0	42.492455	5
TGGGGGT	19580	0.0	42.017876	5
ATGGGTA	4470	0.0	41.93512	4
>>END_MODULE