FastQCFastQC Report
Fri 17 Jun 2016
SRR1527888_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527888_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3396
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC2457.2143698468786805No Hit
CCTAGAGGGACTGAGAGTAAAGACACTAATGAAAATGGCCAATCATCCCC2447.1849234393404No Hit
CCTAGACCTTTAGCAGCAAGGTCCATATCTGACTTTTTGTTAACGTATTT2196.448763250883392No Hit
CCTAGACAAATTAGAGCCAATACCATCAGCTTTACCGTCTTTCCAGAAAT1755.153121319199058No Hit
CCTAGAGGGCGCAGAGTGCAGTGCTCCTAGAGGGGGGGAATTCGTGGAGA1454.269729093050648No Hit
CCTAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAA1263.7102473498233217No Hit
CCTAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA591.7373380447585394No Hit
CCTAGAGGGATTGTTTCATGTGAACATGAGATATTATTTGTGTCAAGTTG571.678445229681979No Hit
CCTAGAGGGAACATTGCCATAAAAGCTCTTATAATCAACCGATTAATGTT461.3545347467608952No Hit
CCTAGAGGAGCGTGAAGTCGAGATCCTGCACGATCAGTTGTTGGCTGCTT431.266195524146054No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTAGAGGGA421.2367491166077738No Hit
CCTAGAGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGC351.0306242638398115No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTT300.88339222614841No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT270.7950530035335688No Hit
CCTAGAGGGAAGATTATGTGCGTCTTATGAAATATGGTGATTCTCTGTAC200.5889281507656066No Hit
CCTAGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTGGTAGCGC200.5889281507656066No Hit
CCTAGAGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA190.5594817432273262No Hit
CCTAGAGGGGGGAATTCCTTCGATCCTGTTTTGCTACCCGTTGTAGCGCC170.5005889281507656No Hit
CCTAGAGGGGTAGAGAATGTGACAAATCCTTTATCCAGCGTTCAAATCTT160.47114252061248524No Hit
CCTAGAGGGGCCCACAACAAGGACGATATGGACAGACTCCTTTCTGCTTG150.441696113074205No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGACAGCATTT140.4122497055359246No Hit
CCTAGAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA130.3828032979976443Illumina Single End Adapter 2 (100% over 34bp)
CCTAGAGGGAAGCAGTGGTATCAACGCAGCTCCTAGAGGGTGGTATCAAC130.3828032979976443No Hit
CCTAGAAGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTT110.32391048292108365No Hit
CCTAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAG110.32391048292108365No Hit
CCTAGAGGGGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTC100.2944640753828033Illumina Single End Adapter 2 (100% over 28bp)
CCTAGAGGGGGGGTATCAACGCAGTGCGGTGCTCCTAGAGAGTGCAGTGG100.2944640753828033No Hit
CCTAGAGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTT90.26501766784452296No Hit
CCTAGAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA90.26501766784452296Illumina Single End Adapter 2 (97% over 34bp)
CCTAGAGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.26501766784452296No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCATTT80.23557126030624262No Hit
CCTAGAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA80.23557126030624262Illumina Single End Adapter 2 (97% over 34bp)
CCTAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA80.23557126030624262Illumina Single End Adapter 2 (100% over 34bp)
CCTAGAGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.23557126030624262No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCCTTT70.2061248527679623No Hit
CCTAGAGGGCAGTGCTCCTAGAGGGGTGGTATCACGCAGAGTGCAGTGGC70.2061248527679623No Hit
CCTAGAGGGCCAGTGCTCCTAGAGGGTGCAGTGCTCCTAGAGGGGGTATC70.2061248527679623No Hit
CCTAGAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.2061248527679623No Hit
CCTAGAGGGGAGTGCAGTGCTCCTAGAGGGGGTATCAACGCAAAAAAAAA70.2061248527679623No Hit
CCTAGAGGGCGGCAGAGTGCAGTGCTCCTAGGGGGGAAAAAAAAAAAAAA70.2061248527679623No Hit
CCTAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAGATCGGAAGAGCTCG60.17667844522968199No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTA60.17667844522968199No Hit
CCTAGAGGGGCTCCTAGAGGGAGTGGTATCAACAAAAAAAAAAAAAAAAA60.17667844522968199No Hit
CCTAGAGGGTCAACACAGAGTGCAGTGCTCCTAGAGGGGAGTACAATGCT60.17667844522968199No Hit
CCTAGAGGGGCAGAGTGCAGTGCTCCTACAGGTATCAACGCAGAGTTCAG60.17667844522968199No Hit
CCTAGAGGGATGCAGTGCTCCTAGAGGGGTGCAGTGCTCCTAGAGGGGCA60.17667844522968199No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTT50.14723203769140164No Hit
CCTAGAGGGCAGTGCTCCTAGAGGGGAGGCAAAAAAAAAAAAAAAAAAAA50.14723203769140164No Hit
CCTAGAGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.14723203769140164No Hit
CCTAGAGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG50.14723203769140164No Hit
CCTAGAGGGATGGTATCAACGCAGAGTGCAGTCCCCTAGGGGGGAAAAAA50.14723203769140164No Hit
CCTAGAGGGGTGCAGTGCTCCTAGAGGGAGTGGTATCAACGCAAAAAAAA50.14723203769140164No Hit
CCTAGAGGGGAGGGCAGTGGTATCAACGCAGAGTGCAGTGCCCCTCTGGG50.14723203769140164No Hit
CCTAGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGC50.14723203769140164No Hit
CCTAGAGGGAAGCGTGGTATCACTAAAGGGGGGAAAAAAAAAAAAAAAAA50.14723203769140164No Hit
CCTAGAGGGGGGAATTCCTTCGATTCTGCTTTGCTACCCGTTGTAGCGCC50.14723203769140164No Hit
CCTAGAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA50.14723203769140164Illumina Single End Adapter 2 (100% over 34bp)
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTC50.14723203769140164No Hit
CCTAGAGGGGAGTGCAGTGCTCCTAGAGGGCAGGTATCAACGCAGAGTGC50.14723203769140164No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTAGAGGGG50.14723203769140164No Hit
CCTAGAGGGGAGTGCAGTGCTCCTAGAGGAGAAAAAAAAAAAAAAAAAAA50.14723203769140164No Hit
CCTAGAGGGGGGGATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC40.11778563015312131No Hit
CCTAGAGGGGTGCAGTGCTCCTAGGGGCAGAGTGCAGTGCTCCTGAGGGG40.11778563015312131No Hit
CCTAGAGGGGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG40.11778563015312131Illumina Single End Adapter 2 (100% over 30bp)
CCTAGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.11778563015312131No Hit
CCTAGAGGGGTGGTATCAACGCAAAAAAAAAAAAGTACGATGAGTCCGGC40.11778563015312131No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG40.11778563015312131Illumina Single End Adapter 2 (100% over 23bp)
CCTAGAGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCC40.11778563015312131Illumina Single End Adapter 2 (95% over 22bp)
CCTAGAGGGTATCAACGCAGAGTGCAGTCTCCTAGAGGGCAGTGCTCCTA40.11778563015312131No Hit
CCTAGAGGGGAGTGCAGTGCTCCTAGAGGGGTGGTATCAACGCAAAAAAA40.11778563015312131No Hit
CCTAGAAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT40.11778563015312131No Hit
CCTAGAGGGGCAGAGTGCAGTGCTCCCTCTAGGAGCACGGCCACCGCCTG40.11778563015312131No Hit
CCTAGAGGGCAGTGCTCCTAGAGGGATGGTATCAACGCAAAAAAAAAAAA40.11778563015312131No Hit
CCTAGAGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA40.11778563015312131Illumina Single End Adapter 2 (97% over 34bp)
CCTAGAGGGGGAGCAGGATATCAACGCAAAAAAAAAAAAAAAAAAAAAAA40.11778563015312131No Hit
CCTAGAGGGGCTGCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAA40.11778563015312131No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGACAGCATTT40.11778563015312131No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTAGAGGGC40.11778563015312131No Hit
CCTAGAGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA40.11778563015312131Illumina Single End Adapter 2 (100% over 34bp)
CCTAGAGATCGTCTTCTCCAAAGTCAAGGCGAAATCTGCTAGAGTAATCA40.11778563015312131No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGAGATCGGAAGAGCTCGTAT40.11778563015312131No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGCT253.561894E-544.038
GTTGATG253.561894E-544.036
TGGTTGA253.561894E-544.034
GGAGAAA452.4374458E-1044.020
GAGAAAG452.4374458E-1044.021
GGTTGAT253.561894E-544.035
GAAATGG452.4374458E-1044.027
CTCCTCT253.561894E-544.034
AACTGGT253.561894E-544.031
GGCGCAG253.561894E-544.08
GGCAACA253.561894E-544.022
GATGCTG253.561894E-544.039
TCGGCAA253.561894E-544.020
GAAGCAG600.044.09
ATGGCTC452.4374458E-1044.030
AGGGCGC253.561894E-544.06
TGCTGAA253.561894E-544.041
CAACATT253.561894E-544.024
TGGAGAA452.4374458E-1044.019
GCAACAT253.561894E-544.023
Produced by FastQC (version 0.11.5)