FastQCFastQC Report
Fri 17 Jun 2016
SRR1527887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4605
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGACTGAGAGTAAAGACACTAATGAAAATGGCCAATCATCCCCGC3858.360477741585234No Hit
CCTAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG3477.535287730727471No Hit
CCTAGAGGGCGCAGAGTGCAGTGCTCCTAGAGGGGGGGAATTCGTGGAGAAA2465.342019543973942No Hit
CCTAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA2164.690553745928339No Hit
CCTAGACCTTTAGCAGCAAGGTCCATATCTGACTTTTTGTTAACGTATTTAG1503.257328990228013No Hit
CCTAGACAAATTAGAGCCAATACCATCAGCTTTACCGTCTTTCCAGAAATTG1172.5407166123778504No Hit
CCTAGAGGGAACATTGCCATAAAAGCTCTTATAATCAACCGATTAATGTTTT721.5635179153094463No Hit
CCTAGAGGGATTGTTTCATGTGAACATGAGATATTATTTGTGTCAAGTTGAC651.4115092290988056No Hit
CCTAGAGGAGCGTGAAGTCGAGATCCTGCACGATCAGTTGTTGGCTGCTTTT621.3463626492942453No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTAGAGGGAAG601.3029315960912053No Hit
CCTAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA551.1943539630836049No Hit
CCTAGAGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG511.1074918566775245No Hit
CCTAGAGGGGGGAATTCCTTCGATCCTGTTTTGCTACCCGTTGTAGCGCCGG410.8903365906623236No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA320.6948968512486429No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA310.6731813246471227No Hit
CCTAGAGGGGGGGTATCAACGCAGTGCGGTGCTCCTAGAGAGTGCAGTGGTA310.6731813246471227No Hit
CCTAGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTGGTAGCGCCG240.5211726384364821No Hit
CCTAGAGGGAAGATTATGTGCGTCTTATGAAATATGGTGATTCTCTGTACCG240.5211726384364821No Hit
CCTAGAGGGGCCCACAACAAGGACGATATGGACAGACTCCTTTCTGCTTGCG230.499457111834962No Hit
CCTAGAGGGGTAGAGAATGTGACAAATCCTTTATCCAGCGTTCAAATCTTAT220.47774158523344196No Hit
CCTAGAGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.32573289902280134No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGCTCCTAGAGGGTGGTATCAACGC140.30401737242128124No Hit
CCTAGAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.30401737242128124No Hit
CCTAGAGGGCAGTGCTCCTAGAGGGGTGGTATCACGCAGAGTGCAGTGGCTG130.28230184581976114No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGA130.28230184581976114No Hit
CCTAGAGGGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAG120.26058631921824105No Hit
CCTAGAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTAT110.23887079261672098No Hit
CCTAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG110.23887079261672098No Hit
CCTAGAGGGATGGTATCAACGCAGAGTGCAGTCCCCTAGGGGGGAAAAAAAA100.21715526601520088No Hit
CCTAGAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA100.21715526601520088Illumina Single End Adapter 2 (97% over 34bp)
CCTAGAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA100.21715526601520088Illumina Single End Adapter 2 (100% over 34bp)
CCTAGAGGGATGCAGTGCTCCTAGAGGGGTGCAGTGCTCCTAGAGGGGCAAA100.21715526601520088No Hit
CCTAGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG90.19543973941368079No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTAGAGGGGAG90.19543973941368079No Hit
CCTAGAGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA90.19543973941368079Illumina Single End Adapter 2 (100% over 34bp)
CCTAGAAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA80.17372421281216072No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTTA80.17372421281216072No Hit
CCTAGAGGGTATCAACGCAGAGTGCAGTCTCCTAGAGGGCAGTGCTCCTAGA80.17372421281216072No Hit
CCTAGAAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA80.17372421281216072No Hit
CCTAGAGGGGCAGAGTGCAGTGCTCCTACAGGTATCAACGCAGAGTTCAGTG80.17372421281216072No Hit
CCTAGAGGGTCAACACAGAGTGCAGTGCTCCTAGAGGGGAGTACAATGCTCC80.17372421281216072No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGACAGCATTTTA80.17372421281216072No Hit
CCTAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG70.15200868621064062No Hit
CCTAGAGGGGTATCAACGCAGAGTCTCCTTTCTTTTTTTTTTTTTTTTTTTT70.15200868621064062No Hit
CCTAGAGGGCGCAGAGTGCAGTGCTCCTAGAGGGGGGGGATTCGTGGAGAAA70.15200868621064062No Hit
CCTAGAGGGGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG70.15200868621064062Illumina Single End Adapter 2 (100% over 30bp)
CCTAGAGGTATCAACGCAGAGTCGATCTGGAACATCTTCTCACCCACGAAGA70.15200868621064062No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGGAGCATTTTA70.15200868621064062No Hit
CCTAGAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA70.15200868621064062Illumina Single End Adapter 2 (97% over 34bp)
CCTAGAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC70.15200868621064062Illumina Single End Adapter 2 (100% over 25bp)
CCTAGAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA70.15200868621064062Illumina Single End Adapter 2 (97% over 34bp)
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCATCCTAGAGAAGCAGTGG60.13029315960912052No Hit
CCTAGAGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTTAT60.13029315960912052No Hit
CCTAGAGGGAGAGTGCAGTGCTCCTAGAGGGGTGCTCCTAGAGGGGAGTCGA60.13029315960912052No Hit
CCTAGAGGAGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT60.13029315960912052No Hit
CCTAGAGGGAAGCGTGGTATCACTAAAGGGGGGAAAAAAAAAAAAAAAAAAA60.13029315960912052No Hit
CCTAGAGGGGCAGAGTGCAATGCTTTGTGGGGAAAAAAAAAAAAAAAAAAAA60.13029315960912052No Hit
CCTAGAGGGGAGGGCAGTGGTATCAACGCAGAGTGCAGTGCCCCTCTGGGAG60.13029315960912052No Hit
CCTAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA60.13029315960912052Illumina Single End Adapter 2 (100% over 34bp)
CCTAGAGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13029315960912052No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTGA60.13029315960912052No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGACAGCATTTGA60.13029315960912052No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA50.10857763300760044No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCCTTTGA50.10857763300760044No Hit
CCTAGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10857763300760044No Hit
CCTAGAGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG50.10857763300760044No Hit
CCTAGAGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA50.10857763300760044Illumina Single End Adapter 2 (100% over 34bp)
CCTAGAGGGACAAAGGATCAGAGCTGCAGACTTTTGGAATGGGTATATGGTC50.10857763300760044No Hit
CCTAGAGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTT50.10857763300760044Illumina Single End Adapter 2 (100% over 27bp)
CCTAGAGGGGAGTGCAGTGCTCCTAGAGGGCAGGTATCAACGCAGAGTGCAG50.10857763300760044No Hit
CCTAGAGGGGCATCTAGACTGACTGAAAAAATGCCGTGCGAAAAAACATGTG50.10857763300760044No Hit
CCTAGAGGGGTGGTATCAACGCAAAAAAAAAAGCCTTGGCCTTGGCAATGCT50.10857763300760044No Hit
CCTAGAGGGGAGTGCAGTGCTCCTAGAGGGGTGGTATCAACGCAAAAAAAAA50.10857763300760044No Hit
CCTAGAGGGAAGCATGGTATCAACGCAGAGTGCAGTGCTCCTAGGGGCACTG50.10857763300760044No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCCTTTTA50.10857763300760044No Hit
CCTAGAGGGGAGTGCAGTGCTCCTAGAGGGGGTATCAACGCAAAAAAAAAAA50.10857763300760044No Hit
CCTAGAGGGGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC50.10857763300760044Illumina Single End Adapter 2 (96% over 31bp)
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTAGAGGGAAG50.10857763300760044No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT50.10857763300760044No Hit
CCTAGAGGGGTGCAGTGCTCCTAGAGGGAGTGGTATCAACGCAAAAAAAAAA50.10857763300760044No Hit
CCTAGAGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.10857763300760044No Hit
CCTAGAGGGCAGTGCTCCTAGAGGGATGGTATCAACGCAAAAAAAAAAAAAA50.10857763300760044No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCAG357.55208E-846.0000048
AGGGCGC357.55208E-846.0000046
GCGCAGA357.55208E-846.0000049
GGGCGCA357.55208E-846.0000047
GAGGGCG357.55208E-846.0000045
AAAAACA357.55208E-846.00000446
TAGAAGG205.6756E-446.03
TGATGCT301.487324E-646.038
ATCCCCG301.487324E-646.045
GTTGATG301.487324E-646.036
ACTAATG301.487324E-646.025
TGGTTGA301.487324E-646.034
GGTTGAT301.487324E-646.035
CCAATCA301.487324E-646.039
CTGAGAG301.487324E-646.011
AGAAGGG205.6756E-446.04
ACTGAGA301.487324E-646.010
AATCATC301.487324E-646.041
AACTGGT301.487324E-646.031
GGACTGA301.487324E-646.08
Produced by FastQC (version 0.11.5)