FastQCFastQC Report
Fri 17 Jun 2016
SRR1527886_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527886_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4594
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGACTGAGAGTAAAGACACTAATGAAAATGGCCAATCATCCCCGC3758.162821070962124No Hit
CCTAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG3377.335655202437962No Hit
CCTAGAGGGCGCAGAGTGCAGTGCTCCTAGAGGGGGGGAATTCGTGGAGAAA2144.658249891162386No Hit
CCTAGAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA1733.765781454070527No Hit
CCTAGACCTTTAGCAGCAAGGTCCATATCTGACTTTTTGTTAACGTATTTAG1172.546800174140183No Hit
CCTAGACAAATTAGAGCCAATACCATCAGCTTTACCGTCTTTCCAGAAATTG1042.2638223770134958No Hit
CCTAGAGGAGCGTGAAGTCGAGATCCTGCACGATCAGTTGTTGGCTGCTTTT731.5890291684806268No Hit
CCTAGAGGGATTGTTTCATGTGAACATGAGATATTATTTGTGTCAAGTTGAC731.5890291684806268No Hit
CCTAGAGGGAACATTGCCATAAAAGCTCTTATAATCAACCGATTAATGTTTT611.3278188942098388No Hit
CCTAGAGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG601.30605137135394No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTAGAGGGAAG591.2842838484980408No Hit
CCTAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA531.153678711362647No Hit
CCTAGAGGGGTAGAGAATGTGACAAATCCTTTATCCAGCGTTCAAATCTTAT390.848933391380061No Hit
CCTAGAGGGAAGATTATGTGCGTCTTATGAAATATGGTGATTCTCTGTACCG340.740095777100566No Hit
CCTAGAGGGGGGGTATCAACGCAGTGCGGTGCTCCTAGAGAGTGCAGTGGTA320.696560731388768No Hit
CCTAGAGGGGGGAATTCCTTCGATCCTGTTTTGCTACCCGTTGTAGCGCCGG310.674793208532869No Hit
CCTAGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTGGTAGCGCCG280.6094906399651719No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA210.457117979973879No Hit
CCTAGAGGGGCCCACAACAAGGACGATATGGACAGACTCCTTTCTGCTTGCG210.457117979973879No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA190.413582934262081No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTAGAGGGGAG190.413582934262081No Hit
CCTAGAGGGCAGTGCTCCTAGAGGGGTGGTATCACGCAGAGTGCAGTGGCTG180.391815411406182No Hit
CCTAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG160.348280365694384No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC160.348280365694384Illumina Single End Adapter 2 (100% over 25bp)
CCTAGAGGGTCAACACAGAGTGCAGTGCTCCTAGAGGGGAGTACAATGCTCC150.326512842838485No Hit
CCTAGAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTAT130.28297779712668697No Hit
CCTAGAGGGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAG120.26121027427078797No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGCTCCTAGAGGGTGGTATCAACGC120.26121027427078797No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGACAGCATTTTA120.26121027427078797No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGA120.26121027427078797No Hit
CCTAGAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.21767522855899No Hit
CCTAGAGGGATGCAGTGCTCCTAGAGGGGTGCAGTGCTCCTAGAGGGGCAAA100.21767522855899No Hit
CCTAGAGGGGCTCCTAGAGGGAGTGGTATCAACAAAAAAAAAAAAAAAAAAA90.195907705703091No Hit
CCTAGAGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.195907705703091No Hit
CCTAGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTTA80.174140182847192No Hit
CCTAGAGGGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA80.174140182847192No Hit
CCTAGAGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.174140182847192No Hit
CCTAGATTGAGAAGCTGGTCTAAAGGTGTAGACTGGTGCTACACCAGCTGTG70.15237265999129299No Hit
CCTAGAGCTCGTATCTCTAGCTGCATATGTAGCTGAAGATGGCCTAGCCAGC70.15237265999129299No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT70.15237265999129299No Hit
CCTAGAGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.15237265999129299No Hit
CCTAGAGGGGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAAA60.13060513713539398No Hit
CCTAGAAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA60.13060513713539398No Hit
CCTAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.13060513713539398No Hit
CCTAGAGGGGCAGAGTGCAGTGCTCCCTCTAGGAGCACGGCCACCGCCTGAC60.13060513713539398No Hit
CCTAGAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA60.13060513713539398Illumina Single End Adapter 2 (100% over 34bp)
CCTAGAGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTTAT60.13060513713539398No Hit
CCTAGAAGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA60.13060513713539398No Hit
CCTAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG60.13060513713539398No Hit
CCTAGAGGGGTGGGCTTCACCCAGATGACTATCTGGGCCAAGGGCAAACACC60.13060513713539398No Hit
CCTAGAGGGGTGGTATCAACGCAAAAAGTCCTCCCCAGAGTGGAAGCCTTTC60.13060513713539398No Hit
CCTAGAGGGCGCAGAGTGCAGTGCTCCTAGAGGGGGGGGATTCGTGGAGAAA60.13060513713539398No Hit
CCTAGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG60.13060513713539398No Hit
CCTAGAGGGACAAAGGATCAGAGCTGCAGACTTTTGGAATGGGTATATGGTC60.13060513713539398No Hit
CCTAGAGGGGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG60.13060513713539398Illumina Single End Adapter 2 (100% over 30bp)
CCTAGAGGGAAGCGTGGTATCACTAAAGGGGGGAAAAAAAAAAAAAAAAAAA60.13060513713539398No Hit
CCTAGAGGGGAGTGCAGTGCTCCTAGAAAAAAAAAAAAAAAAAAAAAAAAAA60.13060513713539398No Hit
CCTAGAGATCGTCTTCTCCAAAGTCAAGGCGAAATCTGCTAGAGTAATCAAC60.13060513713539398No Hit
CCTAGAGGGAAGCATGGTATCAACGCAGAGTGCAGTGCTCCTAGGGGCACTG60.13060513713539398No Hit
CCTAGAGGGGTGCAGTGCTCCTAGAGGGAGTGGTATCAACGCAAAAAAAAAA60.13060513713539398No Hit
CCTAGAGGGGGTGGCAGAGCTGGGAGTCCGGCCTGGAGGGGGTCCTGTCAGT50.108837614279495No Hit
CCTAGAGGGAGCACTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAA50.108837614279495No Hit
CCTAGAGGGAGAGTGCAGTGCTCCTAGAGGGGTGCTCCTAGAGGGGAGTCGA50.108837614279495No Hit
CCTAGAGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.108837614279495No Hit
CCTAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAGATCGGAAGAGCTCG50.108837614279495No Hit
CCTAGAGGGGAGTGCAGTGCTCCTAGAGGAGAAAAAAAAAAAAAAAAAAAAA50.108837614279495No Hit
CCTAGAGGGGAGTGCAGTGCTCCTAGAGGGGTGGTATCAACGCAAAAAAAAA50.108837614279495No Hit
CCTAGAGGGAACATTGCCATAAAAGCTCTTATAATCAACCGATTAATGTTCT50.108837614279495No Hit
CCTAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA50.108837614279495Illumina Single End Adapter 2 (100% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCCG357.5262506E-846.00000445
ACTAATG357.5262506E-846.00000425
CCAATCA357.5262506E-846.00000439
CTGAGAG357.5262506E-846.00000411
ACTGAGA357.5262506E-846.00000410
AATCATC357.5262506E-846.00000441
GGACTGA357.5262506E-846.0000048
GAAAATG357.5262506E-846.00000431
CATCCCC357.5262506E-846.00000444
ATGGCCA357.5262506E-846.00000435
ACACTAA357.5262506E-846.00000423
CAATCAT357.5262506E-846.00000440
ATGAAAA357.5262506E-846.00000429
TAATGAA357.5262506E-846.00000427
GACACTA357.5262506E-846.00000422
AGGGACT357.5262506E-846.0000046
AAGACAC357.5262506E-846.00000420
GAGTAAA357.5262506E-846.00000415
CTAATGA357.5262506E-846.00000426
AGTAAAG357.5262506E-846.00000416
Produced by FastQC (version 0.11.5)