FastQCFastQC Report
Fri 17 Jun 2016
SRR1527885_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527885_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793971
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC58300.7342837458798873No Hit
CTTCTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT30670.38628614899032837No Hit
CTTCTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC20520.2584477266801936No Hit
CTTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC18800.23678446693896882No Hit
CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16760.21109083329239986No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG16240.20454147569621559No Hit
CTTCTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG15840.19950350831453545No Hit
CTTCTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC14230.17922568960327268No Hit
CTTCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG13530.17040924668533233No Hit
CTTCTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG13300.16751241544086623No Hit
CTTCTGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT11810.14874598694410754No Hit
CTTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC11630.14647890162235144No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC11280.14207068016338129No Hit
CTTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT10940.1377884078889531No Hit
CTTCTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA10250.1290979141555548No Hit
CTTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC10220.1287200666019288No Hit
CTTCTGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC10200.1284681682328448No Hit
CTTCTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA10080.1269567780183407No Hit
CTTCTGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT9320.11738463999314837No Hit
CTTCTGGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC9270.11675489407043833No Hit
CTTCTGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA9080.11436185956414024No Hit
CTTCTGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA8860.11159097750421615No Hit
CTTCTGGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCC8720.1098276889206281No Hit
CTTCTGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC8200.10327833132444385No Hit
CTTCTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT8120.10227073784810779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC254.441593E-544.035
AACCGAT207.8550103E-444.044
GATACGA254.441593E-544.09
CGTATGT454.802132E-1044.023
CGTATGA207.8550103E-444.041
CGAAAGG207.8550103E-444.030
TCCGATA302.5267236E-644.029
CCGATAG302.5267236E-644.030
CTATCAC302.5267236E-644.044
ACCGATA207.8550103E-444.026
TTCGTAA454.802132E-1044.042
TTATCGA207.8550103E-444.043
ATCGTAA207.8550103E-444.033
TTATAGT207.8550103E-444.026
CGTTAGA207.8550103E-444.029
TTATAGG207.8550103E-444.024
CGAATCG207.8550103E-444.030
TTAACCG207.8550103E-444.018
TAGGCTA207.8550103E-444.017
CGGTAGG454.802132E-1044.031
Produced by FastQC (version 0.11.5)