##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527885_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	793971
Sequences flagged as poor quality	0
Sequence length	50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62635033269477	34.0	31.0	34.0	31.0	34.0
2	32.90825609499591	34.0	31.0	34.0	31.0	34.0
3	33.216220743578795	34.0	34.0	34.0	31.0	34.0
4	36.438853560142626	37.0	37.0	37.0	35.0	37.0
5	36.46983454055627	37.0	37.0	37.0	35.0	37.0
6	36.370534943971506	37.0	37.0	37.0	35.0	37.0
7	36.65825200164741	37.0	37.0	37.0	35.0	37.0
8	36.71829575639413	37.0	37.0	37.0	35.0	37.0
9	38.673820580348654	39.0	39.0	39.0	39.0	39.0
10	38.20919907654058	39.0	39.0	39.0	37.0	39.0
11	37.264158262707326	39.0	35.0	39.0	35.0	39.0
12	37.0575424039417	39.0	35.0	39.0	34.0	39.0
13	36.99594569574959	39.0	35.0	39.0	33.0	39.0
14	37.82688410533886	40.0	36.0	40.0	34.0	40.0
15	37.93179599758681	40.0	36.0	40.0	34.0	40.0
16	37.97966928263123	40.0	36.0	40.0	34.0	40.0
17	37.87181773641606	40.0	36.0	40.0	34.0	40.0
18	37.8517729236962	40.0	36.0	40.0	34.0	40.0
19	37.827358933764586	40.0	36.0	40.0	34.0	40.0
20	37.705334325812906	40.0	36.0	40.0	34.0	40.0
21	37.7213009039373	40.0	36.0	40.0	34.0	40.0
22	37.749471958043806	40.0	36.0	40.0	34.0	40.0
23	37.67791518833811	40.0	35.0	40.0	34.0	40.0
24	37.62011584805994	40.0	35.0	40.0	34.0	40.0
25	37.569603927599374	40.0	35.0	40.0	34.0	40.0
26	37.466622836350446	40.0	35.0	40.0	34.0	40.0
27	37.34100741714748	40.0	35.0	40.0	33.0	40.0
28	37.19940148947506	40.0	35.0	40.0	33.0	40.0
29	37.22528656588213	40.0	35.0	40.0	33.0	40.0
30	37.36976791343764	40.0	35.0	40.0	33.0	40.0
31	37.41732254704517	40.0	35.0	40.0	34.0	40.0
32	37.39489477575378	40.0	35.0	40.0	34.0	40.0
33	37.328874732200546	40.0	35.0	40.0	33.0	40.0
34	37.033937007774846	40.0	35.0	40.0	33.0	40.0
35	37.03708069942101	40.0	35.0	40.0	33.0	40.0
36	36.984059871204366	40.0	35.0	40.0	33.0	40.0
37	36.931083125202306	40.0	35.0	40.0	33.0	40.0
38	36.869480119550964	39.0	35.0	40.0	33.0	40.0
39	36.7437400106553	39.0	35.0	40.0	33.0	40.0
40	36.5243717969548	39.0	35.0	40.0	32.0	40.0
41	36.427602519487486	39.0	35.0	40.0	32.0	40.0
42	36.33488502728689	39.0	35.0	40.0	32.0	40.0
43	36.261573785440525	38.0	35.0	40.0	32.0	40.0
44	35.95242647401479	38.0	35.0	40.0	31.0	40.0
45	35.94605470476881	38.0	35.0	40.0	31.0	40.0
46	35.77675381090745	37.0	35.0	40.0	31.0	40.0
47	35.69419537993201	37.0	35.0	40.0	31.0	40.0
48	35.58215728282267	37.0	35.0	40.0	30.0	40.0
49	35.52821576606702	36.0	35.0	40.0	30.0	40.0
50	35.03838175449733	36.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	1.0
14	2.0
15	9.0
16	36.0
17	61.0
18	128.0
19	258.0
20	450.0
21	664.0
22	1088.0
23	1602.0
24	2108.0
25	3052.0
26	3927.0
27	5054.0
28	6619.0
29	8478.0
30	11027.0
31	14160.0
32	18048.0
33	26190.0
34	63001.0
35	79968.0
36	52876.0
37	97898.0
38	222806.0
39	174457.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	98.83648143320096	0.7446115790123318	0.2399331965525189	0.17897379123418866
8	98.67954874926163	0.9187993012339241	0.23502117835538075	0.16663077114907218
9	93.77773243607133	2.6785613076548134	2.4709970515295896	1.072709204744254
10	52.829259506959325	32.6226020849628	6.952898783456826	7.59523962462105
11	34.20641308057851	24.518149907238428	23.073008963803467	18.202428048379602
12	29.53646921613006	17.63439722609516	26.388999094425365	26.44013446334942
13	23.429445156057337	19.0869691714181	29.736854368736388	27.746731303788174
14	24.024806951387394	19.97730395694553	30.192664467593904	25.80522462407317
15	25.736708267682324	20.16144166474594	27.668516860187587	26.433333207384145
16	30.105507631890838	19.844805414807343	25.07207442085416	24.97761253244766
17	28.059715027375056	20.54206010043188	25.405713810705933	25.992511061487132
18	28.646512278156255	20.34431988070093	24.191185824167384	26.817982016975435
19	28.274962183757342	21.510105532821726	23.88261032203947	26.33232196138146
20	29.982203380224213	20.95668481594416	23.830089512085454	25.231022291746175
21	30.94571464197055	19.41179211835193	26.521245738194466	23.12124750148305
22	29.1042116147819	18.479768152741094	25.868954911451425	26.54706532102558
23	28.34335259096365	20.69735544497217	24.68301738980391	26.276274574260267
24	28.271057759036538	21.11714407705067	25.10091678411428	25.510881379798505
25	28.256321704445124	21.639329396161823	23.28447764464949	26.81987125474356
26	28.047623905659023	21.868808810397358	24.363484308620844	25.72008297532278
27	28.198007232002176	19.90437937909571	23.716861195182194	28.180752193719922
28	28.463004316278557	22.681432949062373	22.408375116975304	26.44718761768377
29	29.23796964876551	21.393224689566747	23.26571121615273	26.103094445515012
30	27.68614974602347	21.264252724595735	25.13026294411257	25.919334585268228
31	29.732446147277418	21.960247918374854	22.670601319191757	25.63670461515597
32	31.084006846597674	22.244641177070697	22.317817653289605	24.353534323042027
33	30.693186526963835	21.633661682857433	23.281077016666856	24.39207477351188
34	27.687787085422517	23.495946325495513	25.531537046063395	23.284729543018575
35	28.06235996025044	24.496360698312657	24.673445251778716	22.767834089658187
36	28.39398416314954	23.78411805972762	22.876150388364312	24.945747388758534
37	28.40305250443656	23.560306358796478	23.103992463200797	24.932648673566163
38	29.526393281366698	24.247862957211282	22.762544223907422	23.463199537514594
39	30.332216164066445	22.694279765885657	22.058866129871245	24.914637940176657
40	27.4100691335074	22.9810660590878	23.695071986256426	25.91379282114838
41	25.638971700477725	23.439521090820698	24.518527754792053	26.402979453909523
42	26.44983255055915	21.622200307064112	24.587421958736527	27.34054518364021
43	27.192302993434268	20.361952766536813	25.537330708552325	26.908413531476587
44	26.135337436757766	21.481515067930694	24.854308280781037	27.528839214530503
45	26.989272907952554	20.666875742313007	25.211626117326702	27.13222523240773
46	27.09670756236689	23.012805253592386	23.626051833127406	26.264435350913324
47	25.80887715042489	23.277298541130595	24.055160704862015	26.8586636035825
48	27.100360088718606	22.121714772957702	26.296930240525157	24.48099489779853
49	27.815373609363565	22.246404465654287	24.29421225712274	25.644009667859407
50	25.087314272183747	22.14224449003805	24.529485333847205	28.240955903931
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	5.0
6	10.0
7	8.5
8	7.0
9	53.0
10	99.0
11	334.5
12	570.0
13	838.0
14	1106.0
15	1545.5
16	1985.0
17	1667.0
18	1349.0
19	1477.0
20	1605.0
21	1850.0
22	2095.0
23	2229.5
24	2364.0
25	2482.0
26	2600.0
27	2876.5
28	3153.0
29	4052.5
30	4952.0
31	5450.0
32	5948.0
33	7146.0
34	8344.0
35	10932.5
36	13521.0
37	12645.0
38	11769.0
39	13555.0
40	15341.0
41	18027.5
42	20714.0
43	22076.5
44	23439.0
45	24528.5
46	25618.0
47	29282.0
48	32946.0
49	35322.5
50	37699.0
51	41560.0
52	45421.0
53	45821.0
54	46221.0
55	49585.0
56	52949.0
57	63340.5
58	73732.0
59	71537.0
60	69342.0
61	69749.5
62	70157.0
63	69545.0
64	68933.0
65	59930.0
66	50927.0
67	42449.5
68	33972.0
69	28022.5
70	22073.0
71	18729.5
72	15386.0
73	12717.0
74	10048.0
75	10257.0
76	10466.0
77	7827.0
78	5188.0
79	3186.5
80	1185.0
81	837.0
82	489.0
83	335.0
84	181.0
85	116.0
86	51.0
87	33.0
88	15.0
89	8.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	793971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.072660084562282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.83487133318329	6.933502609037358
2	8.696576387325882	1.751953156979285
3	3.231050091279666	0.9763580785696203
4	1.5204941606021958	0.6126168336123108
5	0.840273088753845	0.4231892600611358
6	0.5551804336409333	0.3355286276199005
7	0.39762922949958734	0.2803628847905024
8	0.3626178508015105	0.29220210813745084
9	0.3101007827543952	0.28111857989775446
>10	9.565608822867432	28.380759498772623
>50	4.101332933203291	28.161481968484996
>100	1.5254957861304925	25.295508274231675
>500	0.03626178508015105	2.4390059586559207
>1k	0.021256908495260962	3.1021284152695756
>5k	0.001250406382074174	0.7342837458798873
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5830	0.7342837458798873	No Hit
CTTCTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3067	0.38628614899032837	No Hit
CTTCTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2052	0.2584477266801936	No Hit
CTTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1880	0.23678446693896882	No Hit
CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	1676	0.21109083329239986	No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1624	0.20454147569621559	No Hit
CTTCTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1584	0.19950350831453545	No Hit
CTTCTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	1423	0.17922568960327268	No Hit
CTTCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1353	0.17040924668533233	No Hit
CTTCTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1330	0.16751241544086623	No Hit
CTTCTGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	1181	0.14874598694410754	No Hit
CTTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	1163	0.14647890162235144	No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	1128	0.14207068016338129	No Hit
CTTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	1094	0.1377884078889531	No Hit
CTTCTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1025	0.1290979141555548	No Hit
CTTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	1022	0.1287200666019288	No Hit
CTTCTGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	1020	0.1284681682328448	No Hit
CTTCTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	1008	0.1269567780183407	No Hit
CTTCTGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	932	0.11738463999314837	No Hit
CTTCTGGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	927	0.11675489407043833	No Hit
CTTCTGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	908	0.11436185956414024	No Hit
CTTCTGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA	886	0.11159097750421615	No Hit
CTTCTGGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCC	872	0.1098276889206281	No Hit
CTTCTGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	820	0.10327833132444385	No Hit
CTTCTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	812	0.10227073784810779	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0062974592271002345	0.0	0.0	0.0	0.0
8	0.008564544548856318	0.0	0.0	0.0	0.0
9	0.013602511930536505	0.0	0.0	0.0	0.0
10	0.03262083879637921	0.0	0.0	0.0	0.0
11	0.048238537679587794	0.0	0.0	0.0	0.0
12	0.056299285490276095	0.0	0.0	0.0	0.0
13	0.06347838900917036	0.0	0.0	0.0	0.0
14	0.07922203707692095	0.0	0.0	0.0	0.0
15	0.09320239656108346	0.0	0.0	0.0	0.0
16	0.107560603598872	0.0	0.0	0.0	0.0
17	0.12028147123761447	0.0	0.0	0.0	0.0
18	0.13325423724544094	0.0	0.0	0.0	0.0
19	0.1530282592185357	0.0	0.0	0.0	0.0
20	0.16562317767273615	0.0	0.0	0.0	0.0
21	0.1770845534660586	0.0	0.0	0.0	0.0
22	0.1909389637656791	0.0	0.0	0.0	0.0
23	0.20542311998800963	0.0	0.0	0.0	0.0
24	0.22116676805576022	0.0	0.0	0.0	0.0
25	0.23376168650996068	0.0	0.0	0.0	0.0
26	0.24963128376225327	0.0	0.0	0.0	0.0
27	0.2653749318300039	0.0	0.0	0.0	0.0
28	0.28099263071321245	0.0	0.0	0.0	0.0
29	0.2932097016137869	0.0	0.0	0.0	0.0
30	0.30819765457428544	0.0	0.0	0.0	0.0
31	0.3178957417840198	0.0	0.0	0.0	0.0
32	0.3313723045300143	0.0	0.0	0.0	0.0
33	0.3440931721687568	0.0	0.0	0.0	0.0
34	0.36109631208192744	0.0	0.0	0.0	0.0
35	0.3789810962868921	0.0	0.0	0.0	0.0
36	0.39333930332468064	0.0	0.0	0.0	0.0
37	0.4056823234097971	0.0	0.0	0.0	0.0
38	0.4170177500185775	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	25	4.441593E-5	44.0	35
AACCGAT	20	7.8550103E-4	44.0	44
GATACGA	25	4.441593E-5	44.0	9
CGTATGT	45	4.802132E-10	44.0	23
CGTATGA	20	7.8550103E-4	44.0	41
CGAAAGG	20	7.8550103E-4	44.0	30
TCCGATA	30	2.5267236E-6	44.0	29
CCGATAG	30	2.5267236E-6	44.0	30
CTATCAC	30	2.5267236E-6	44.0	44
ACCGATA	20	7.8550103E-4	44.0	26
TTCGTAA	45	4.802132E-10	44.0	42
TTATCGA	20	7.8550103E-4	44.0	43
ATCGTAA	20	7.8550103E-4	44.0	33
TTATAGT	20	7.8550103E-4	44.0	26
CGTTAGA	20	7.8550103E-4	44.0	29
TTATAGG	20	7.8550103E-4	44.0	24
CGAATCG	20	7.8550103E-4	44.0	30
TTAACCG	20	7.8550103E-4	44.0	18
TAGGCTA	20	7.8550103E-4	44.0	17
CGGTAGG	45	4.802132E-10	44.0	31
>>END_MODULE