FastQCFastQC Report
Fri 17 Jun 2016
SRR1527884_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527884_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195543
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC88250.7381583096551108No Hit
CTTCTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG46370.3878572330731726No Hit
CTTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC30020.25109929128437875No Hit
CTTCTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT30010.2510156472832847No Hit
CTTCTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG26950.22542058294850123No Hit
CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT22430.1876134944539845No Hit
CTTCTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG22210.1857733264299151No Hit
CTTCTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG19800.1656151221662458No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT19780.16544783416405767No Hit
CTTCTGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC19370.16201843011920106No Hit
CTTCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC19260.16109834610716636No Hit
CTTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT17730.14830081393977465No Hit
CTTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT17590.14712979792445777No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA17210.14395132588288334No Hit
CTTCTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG16870.1411074298456852No Hit
CTTCTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC16250.13592150177785323No Hit
CTTCTGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA16050.13424862175597196No Hit
CTTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC15910.13307760574065508No Hit
CTTCTGGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT15300.1279753216739172No Hit
CTTCTGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT14090.11785439754153552No Hit
CTTCTGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT13580.11358855348573829No Hit
CTTCTGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA12700.1062278813894607No Hit
CTTCTGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG12480.1043877133653913No Hit
CTTCTGGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT12270.10263118934241595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG206.311556E-446.042
AAATCGA206.311556E-446.014
CGTATCC301.8614664E-646.015
CAACGTA206.311556E-446.030
CGCATCG301.8614664E-646.040
ATTAACG301.8614664E-646.035
CGACACT301.8614664E-646.035
ATCGTAC453.092282E-1046.023
CGTTAGA253.4172397E-546.029
CGCCTAT206.311556E-446.019
CCCGTAA301.8614664E-646.021
CCGTACG253.4172397E-546.045
TACGATA206.311556E-446.045
CGGATAA206.311556E-446.011
CGTACGT253.4172397E-546.046
CGTAAAC206.311556E-446.032
TAGGCGT206.311556E-446.043
CCGCGAA253.4172397E-546.012
ATTATCG206.311556E-446.017
CACGTTA206.311556E-446.037
Produced by FastQC (version 0.11.5)