FastQCFastQC Report
Fri 17 Jun 2016
SRR1527883_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527883_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207051
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC83730.6936740866790219No Hit
CTTCTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG44200.36618171063194516No Hit
CTTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC28690.23768672574729652No Hit
CTTCTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT28510.23619548801169132No Hit
CTTCTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG25200.20877328298472891No Hit
CTTCTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG22370.18532771191938036No Hit
CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT21600.17894852827262478No Hit
CTTCTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG20260.16784709179645266No Hit
CTTCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC18730.1551715710438084No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT18100.14995223896919022No Hit
CTTCTGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC17700.14663837733451196No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA17300.1433245156998337No Hit
CTTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT16640.13785664400261463No Hit
CTTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT16590.13744241129827986No Hit
CTTCTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG15590.12915775721158426No Hit
CTTCTGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA14940.12377273205523213No Hit
CTTCTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC14750.12219864777875997No Hit
CTTCTGGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT14550.12054171696142087No Hit
CTTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC13930.11540523142766959No Hit
CTTCTGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT13700.1134997609877296No Hit
CTTCTGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT13210.10944028048524876No Hit
CTTCTGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG12250.101487012562021No Hit
CTTCTGGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT12250.101487012562021No Hit
CTTCTGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG12210.10115562639855316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGA351.0196345E-746.00000424
AAATGCG351.0196345E-746.00000417
CGAGTCA253.4172583E-546.032
ATTCGTC301.861481E-646.024
TAAGTCG206.3115807E-446.040
CGCGAAA206.3115807E-446.036
ATCGTAC551.8189894E-1246.023
CCCCGTA206.3115807E-446.017
GTCAATT206.3115807E-446.028
CGTCGTT206.3115807E-446.019
CGTAACT206.3115807E-446.036
TCATACG253.4172583E-546.043
CGATCGA253.4172583E-546.042
CGTCATA206.3115807E-446.034
TATCGCA206.3115807E-446.09
CGTCAAC253.4172583E-546.042
CCGTTCA206.3115807E-446.018
TCGAAAC206.3115807E-446.021
TACGTAA253.4172583E-546.029
TGCGTAA206.3115807E-446.023
Produced by FastQC (version 0.11.5)