FastQCFastQC Report
Fri 17 Jun 2016
SRR1527882_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527882_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120730
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGA51724.283939368839559No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG7850.6502112151080924Illumina Single End Adapter 2 (100% over 23bp)
GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7130.5905740081172864No Hit
GTTGCCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6920.5731798227449681Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGG5680.4704712995941357No Hit
GTTGCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4640.38432866727408266Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGTGCCGGGA4380.36279300919406937No Hit
GTTGCCGGGGGGGCTGGAGAGATGGCTCATCAGTTAAGAGCACTGACTGC4030.3338027002402054No Hit
GTTGCCGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTC3930.3255197548248157Illumina Single End Adapter 2 (100% over 25bp)
GTTGCCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3830.317236809409426Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGC3790.3139236312432701No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG3650.3023275076617245No Hit
GTTGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC3620.2998426240371076No Hit
GTTGCCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG3530.2923879731632569No Hit
GTTGCCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGAAG3470.287418205914023No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGC3440.2849333222894061Illumina Single End Adapter 2 (100% over 21bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCC3430.28410502774786717Illumina Single End Adapter 2 (95% over 22bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATGCCGGGA3290.27250890416632156No Hit
GTTGCCGGGGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAG3110.25759960241862007No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGC3090.2559430133355421No Hit
GTTGCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAACACAGATTGT3000.2484883624616914No Hit
GTTGCCGGGCTGCCGATGCTGGCGATTCTGGTGGTACAGACCATCTTCAT2750.2277809989232171No Hit
GTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2630.21784146442474944No Hit
GTTGCCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC2600.21535658080013254No Hit
GTTGCCGGGGGATGCAGGATCAGAGAGGGTGACAATCCCGTCTGTGCCTC2420.20044727905243104No Hit
GTTGCCGGGGCTGTTGCCGGGGTCTCTGCAGAAGCTGGGAGCACAGGTCC2380.19713410088627514No Hit
GTTGCCGGGGCTGTGCCGGGGAGGAACCTGCAGGAGGAGGCGGGGCCGGG2310.19133603909550237No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGT2310.19133603909550237No Hit
GTTGCCGGGGGCCCAGGCCGGTGTGTGTCGGTGTGTATGTGTGTGTGTGT2240.18553797730472957No Hit
GTTGCCGGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAA2210.18305309368011263No Hit
GTTGCCGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTG2170.17973991551395677No Hit
GTTGCCGGGGTACTGTATAAATGAACCAAAACTGTTAAATATGTATTTAG2160.1789116209724178No Hit
GTTGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC2140.17725503188933986No Hit
GTTGCCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA2100.17394185372318396Illumina Single End Adapter 2 (100% over 34bp)
GTTGCCGGGGGCTGTTGCCGGGAGGCCCGGGAGGAAAAGCATCAGAAGCA1960.16234573014163836No Hit
GTTGCCGGGGACCCAAAGGGTAAAGTTTTTGAAATAAACTATTTTATATC1950.1615174356000994No Hit
GTTGCCGGCGTGGCCCTGGGAGCTGGAGCAAGAGTCCACCTTTAGCTGTG1950.1615174356000994No Hit
GTTGCCTGGGATTCTCCCAGCTGCGCCATAGGTCATCCTCAACAACCCAG1910.1582042574339435No Hit
GTTGCCGGGACCCTTCCCACCCCGAGCGAGCCAGCACCACACGGCACAGC1880.1557193738093266No Hit
GTTGCCGGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCC1870.1548910792677876No Hit
GTTGCCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA1870.1548910792677876Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTC1840.15240619564317073No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCCGCTGCCGGGG1820.15074960656009279No Hit
GTTGCCGGGAGCGCAGGCCACTGAGTGGGTTGGAGCCACCACTGAGTGGT1790.14826472293547585No Hit
GTTGCCGGGACGATCGGACCCTGCTTGTAGCTGACCCCCGTCGCTGCGAA1680.13915348297854718No Hit
GTTGCCGATACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGATGGTAT1660.13749689389546924No Hit
GTTGCCGGGTGGCTGCCCTCCACCATATCACACATTCGAGGAACCAACCT1640.1358403048123913No Hit
GTTGCCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTC1630.1350120102708523No Hit
GTTGCCGGGAGCCGGTAGTGGTGGCGCACGGCGGTAGGATTTGCTGAAGG1610.13335542118777438No Hit
GTTGCCGGGGCGCTGTCATTTAAGAAAGAGATAGAAATTTATAAAGTTTA1600.1325271266462354No Hit
GTTGCCATACAAAACAGGGTCGCCAGCAATATCGGTATAAGTCAAAGCAC1590.13169883210469643No Hit
GTTGCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT1580.13087053756315745No Hit
GTTGCCGGGATGCTTAAGGCAAGCATTTCACCTTGTTTTTCAGAGAACTC1570.1300422430216185No Hit
GTTGCCGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTC1540.12755735939700155No Hit
GTTGCCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTT1540.12755735939700155Illumina Single End Adapter 2 (96% over 33bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTA1530.1267290648554626No Hit
GTTGCCGGGAGTTAGTTGTTATAGTTGTCTTTGTTAAGAGATTGTTCCTC1520.12590077031392363No Hit
GTTGCCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC1520.12590077031392363No Hit
GTTGCCGGGGCTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACGC1510.12507247577238467No Hit
GTTGCCGGGGACAACTGTGATTGTCAGTCTCTATTAAAATCCCTCAGTTG1500.1242441812308457No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCCGTTGCCGGGA1490.12341588668930673No Hit
GTTGCCGGGAAGTCCGCTGGCCAGTCCGTCACCGACACCGTCTCCGGCGG1480.12258759214776775No Hit
GTTGCCGGGATTACTGTAGCTCCCTATAGTGTTACGTTCAGACACCGTAG1480.12258759214776775No Hit
GTTGCCGGGGAAGCAAAAGGCAGAGAGAGAGAGAGACAGAGAGAGAGGGA1430.1184461194400729No Hit
GTTGCCGGGAGATGAACTTAGAAATCTACACAAACTGCTTTACAACAGGC1410.11678953035699495No Hit
GTTGCCGGGTGGAGGGTCTGATTTTTTAAGGAAAAGATTGCAGAAAGGGC1400.11596123581545598No Hit
GTTGCCGGGCTTTCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGG1400.11596123581545598No Hit
GTTGCCGGCAACAGTACCCGCAACAGCACTGCACCAAAAAAAAAAAAAAA1380.11430464673237803No Hit
GTTGCCGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTC1380.11430464673237803No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG1380.11430464673237803No Hit
GTTGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1370.11347635219083907No Hit
GTTGCCGGGCTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCC1370.11347635219083907No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGTGCCCGGA1350.11181976310776112No Hit
GTTGCCGGCGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA1340.11099146856622215No Hit
GTTGCCGGGAGTGCTTGTCAAAGATGGTGTTGGTGTAGGAATCTCATGTA1300.10767829040006627No Hit
GTTGCCGGGAGCCCATCACTAGAAAATGACCTTGTCCTGATTTTCAGTTT1300.10767829040006627No Hit
GTTGCCGGGTTACCACTTTTCTTGCCTTTGTTTCTGAATTTTTTTCTGCT1290.1068499958585273No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGT1290.1068499958585273Illumina Single End Adapter 2 (95% over 24bp)
GTTGCCGGGGCTGTTGCCGGGGTAAGACTCTGGGCTGCAATGAATCAATC1280.10602170131698832No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGAGATCGGAAGAGCTCGTAT1280.10602170131698832No Hit
GTTGCCGGGAACACTTTCCACTTTCTGACACCTCATCCTGCTGTACGACT1280.10602170131698832No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCG1270.10519340677544935No Hit
GTTGCCGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAA1250.1035368176923714No Hit
GTTGCCGGGAATCATACAGCAGATAGTTATCCAGAATGACAAAGGGCAGG1250.1035368176923714No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1230.10188022860929345No Hit
GTTGCCGGGGGAAAACGTATTTGTCTTTCTGAAACACATTTAATTCATTT1230.10188022860929345No Hit
GTTGCCGGGGCCCCGCGTCCCTCCTCTCTCCGCGCCGGCTGGTGGTCGTT1230.10188022860929345No Hit
GTTGCCGGGGCTGTTGCCGGGGCTTTGCCGGGCCTTCTCTGTGCTGAGCA1230.10188022860929345No Hit
GTTGCCGGGCTCTTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTA1210.10022363952621552No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGAAC207.828475E-444.00000412
CTCTGAC207.828475E-444.00000434
TAGCGAG207.828475E-444.00000428
TTTTGCG207.828475E-444.00000412
CTTCACC207.828475E-444.00000416
CAAGAGT207.828475E-444.00000429
ACACGGC207.828475E-444.00000439
TGGTTCC207.828475E-444.00000428
CAAGACT207.828475E-444.00000422
AAACTGT408.20728E-944.00000429
AACCGTT207.828475E-444.00000443
TTCACCG207.828475E-444.00000417
TGCACCA207.828475E-444.00000430
CTGAGTA207.828475E-444.00000437
GTCATTT207.828475E-444.00000415
CCAACGA207.828475E-444.00000414
ACTCGCT207.828475E-444.00000443
GTGTTCA207.828475E-444.00000426
GCCTTGA207.828475E-444.00000411
GGGTCAC408.20728E-944.0000047
Produced by FastQC (version 0.11.5)