FastQCFastQC Report
Fri 17 Jun 2016
SRR1527881_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527881_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179788
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGAAG72134.011947404721116No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC9640.5361870647651679Illumina Single End Adapter 2 (100% over 25bp)
GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9130.5078203217122389No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGGAG7750.4310632522749015No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGTGCCGGGAAG6630.36876765968807707No Hit
GTTGCCGGGGGGGCTGGAGAGATGGCTCATCAGTTAAGAGCACTGACTGCTC6520.362649342559014No Hit
GTTGCCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGAAGCA5900.32816428237702183No Hit
GTTGCCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA5720.31815249071128215No Hit
GTTGCCGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTT5590.31092175228602575Illumina Single End Adapter 2 (100% over 27bp)
GTTGCCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5350.2975726967317062Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA5320.29590406478741627No Hit
GTTGCCGGGGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA5140.28589227312167664No Hit
GTTGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG4830.26864974303068057No Hit
GTTGCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAACACAGATTGTTC4640.25808174071684425No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG4510.25085100229158785Illumina Single End Adapter 2 (100% over 23bp)
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTC4340.24139542127394487No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT4170.23193984025630188Illumina Single End Adapter 2 (95% over 24bp)
GTTGCCGGGCTGCCGATGCTGGCGATTCTGGTGGTACAGACCATCTTCATGG4130.2297149976639153No Hit
GTTGCCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG3940.21914699535007898No Hit
GTTGCCGGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGA3790.21080383562862925No Hit
GTTGCCGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGCG3760.20913520368433933No Hit
GTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3710.2063541504438561No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATGCCGGGAAG3620.20134825461098627No Hit
GTTGCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3500.1946737268338265Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGGGATGCAGGATCAGAGAGGGTGACAATCCCGTCTGTGCCTCGG3370.1874429884085701No Hit
GTTGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC3300.18354951387189355No Hit
GTTGCCGGGGTACTGTATAAATGAACCAAAACTGTTAAATATGTATTTAGTT3220.17909982868712038No Hit
GTTGCCGGGGGCTGTTGCCGGGAGGCCCGGGAGGAAAAGCATCAGAAGCAGC3210.17854361803902374No Hit
GTTGCCGGGGACCCAAAGGGTAAAGTTTTTGAAATAAACTATTTTATATCTT3200.17798740739092708No Hit
GTTGCCTGGGATTCTCCCAGCTGCGCCATAGGTCATCCTCAACAACCCAGAG3130.17409393285425057No Hit
GTTGCCGGGAGCGCAGGCCACTGAGTGGGTTGGAGCCACCACTGAGTGGTCC3040.16908803702138073No Hit
GTTGCCGGGGCTGTGCCGGGGAGGAACCTGCAGGAGGAGGCGGGGCCGGGGA2980.16575077313280084No Hit
GTTGCCGGGGCTGTTGCCGGGGTCTCTGCAGAAGCTGGGAGCACAGGTCCTG2970.1651945624847042No Hit
GTTGCCGGGAGCCGGTAGTGGTGGCGCACGGCGGTAGGATTTGCTGAAGGAG2920.162413509244221No Hit
GTTGCCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA2820.1568514027632545Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCCGTTGCCGGGGAG2770.15407034952277127No Hit
GTTGCCGGCGTGGCCCTGGGAGCTGGAGCAAGAGTCCACCTTTAGCTGTGCC2730.15184550693038468No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGTAG2700.15017687498609472No Hit
GTTGCCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA2690.1496206643379981Illumina Single End Adapter 2 (100% over 34bp)
GTTGCCGGGACCCTTCCCACCCCGAGCGAGCCAGCACCACACGGCACAGCTC2650.1473958217456115No Hit
GTTGCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT2580.14350234720893498No Hit
GTTGCCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA2500.13905266202416178Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT2460.13682781943177522No Hit
GTTGCCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTCTT2450.13627160878367856No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGCAA2430.13515918748748526No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT2410.13404676619129197No Hit
GTTGCCGGGACGATCGGACCCTGCTTGTAGCTGACCCCCGTCGCTGCGAATC2410.13404676619129197No Hit
GTTGCCGGGATTACTGTAGCTCCCTATAGTGTTACGTTCAGACACCGTAGCT2380.132378134247002No Hit
GTTGCCGGGGCTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACGCCA2360.13126571295080872No Hit
GTTGCCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG2360.13126571295080872No Hit
GTTGCCGGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCC2330.1295970810065188No Hit
GTTGCCGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCT2330.1295970810065188No Hit
GTTGCCGGGATGCTTAAGGCAAGCATTTCACCTTGTTTTTCAGAGAACTCTA2310.1284846597103255No Hit
GTTGCCGGGTTACCACTTTTCTTGCCTTTGTTTCTGAATTTTTTTCTGCTAA2270.1262598171179389No Hit
GTTGCCGGGCTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTG2270.1262598171179389No Hit
GTTGCCGGGAGTTAGTTGTTATAGTTGTCTTTGTTAAGAGATTGTTCCTCTG2250.1251473958217456No Hit
GTTGCCGGGGACAACTGTGATTGTCAGTCTCTATTAAAATCCCTCAGTTGCA2210.12292255322935904No Hit
GTTGCCGGGGAAGCAAAAGGCAGAGAGAGAGAGAGACAGAGAGAGAGGGAGA2170.12069771063697243No Hit
GTTGCCGGGCTGTTGCCGGGGATAGAATGGAAGGAAGGAAGGAAGGAAGGAA2110.11736044674839255No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCT2070.11513560415600597Illumina Single End Adapter 2 (96% over 26bp)
GTTGCCAAGGACATTTATAATGTAGTAACACTTCTGAAAAATAAACATAGAA2070.11513560415600597No Hit
GTTGCCGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGG2020.11235455091552272No Hit
GTTGCCGGGGCTGTTGCCGGGGCTTTGCCGGGCCTTCTCTGTGCTGAGCACC2010.11179834026742608No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATG1980.11012970832313615No Hit
GTTGCCGGCGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG1980.11012970832313615No Hit
GTTGCCGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTAT1960.10901728702694286No Hit
GTTGCCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA1960.10901728702694286Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGGCCCTTCAGCCTAGAGCGCGGAGGAGGACGGTAGCTCGGTCAC1950.1084610763788462No Hit
GTTGCCGGGAGATGAACTTAGAAATCTACACAAACTGCTTTACAACAGGCCG1950.1084610763788462No Hit
GTTGCCGGGGCTGTTGCCGGGGTAAGACTCTGGGCTGCAATGAATCAATCAA1920.10679244443455625No Hit
GTTGCCGGGAAGTCCGCTGGCCAGTCCGTCACCGACACCGTCTCCGGCGGCA1900.10568002313836296No Hit
GTTGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1900.10568002313836296No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT1890.10512381249026632No Hit
GTTGCCGGGGCGCTGTCATTTAAGAAAGAGATAGAAATTTATAAAGTTTAAG1890.10512381249026632No Hit
GTTGCCGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCC1880.10456760184216966No Hit
GTTGCCGGGTGGCTGCCCTCCACCATATCACACATTCGAGGAACCAACCTAT1880.10456760184216966No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTA1850.10289896989787972No Hit
GTTGCCGGGATTGAGAGAGTGGTGTGAGACGGGCAACTTAAATATCATTGAT1850.10289896989787972No Hit
GTTGCCGGGCTGTTGCCGGGGATCAAGGCGCCGCCCCACCTGCCCGGCCCGG1850.10289896989787972No Hit
GTTGCCGGGGGCCCAGGCCGGTGTGTGTCGGTGTGTATGTGTGTGTGTGTGT1840.10234275924978306No Hit
GTTGCCGGGAATCATACAGCAGATAGTTATCCAGAATGACAAAGGGCAGGAT1840.10234275924978306No Hit
GTTGCCGGGGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTGTCCCC1830.10178654860168643No Hit
GTTGCCGGGAGTGCTTGTCAAAGATGGTGTTGGTGTAGGAATCTCATGTATG1830.10178654860168643No Hit
GTTGCCGGGCTTTCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCC1830.10178654860168643No Hit
GTTGCCGGTGACGAAGCTGGGCCCTCCATCGTCCACCGCAAATGCTTCTAAG1820.1012303379535898No Hit
GTTGCCGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG1810.10067412730549313No Hit
GTTGCCGGGAAGGTTTTGAATGCAATTAGGTTATGCTATTTGGACAATAAAC1800.1001179166573965No Hit
GTTGCCGGGCAACAGAGATCTCCGAGATCGACTGGAGACAGCCAACAGGCAG1800.1001179166573965No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCAT351.0131407E-746.00000430
ACTATTT551.8189894E-1246.00000438
AAACTAT551.8189894E-1246.00000436
AACAACC351.0131407E-746.00000441
ACAAATA351.0131407E-746.00000434
ACCGGCT351.0131407E-746.00000435
CTGTATA351.0131407E-746.00000413
ATAGGTC351.0131407E-746.00000428
CCCTAGC351.0131407E-746.00000422
ATAATTA351.0131407E-746.00000440
TAGGTCA351.0131407E-746.00000429
TGTGGCT351.0131407E-746.00000430
GGACTAA351.0131407E-746.0000049
GACCGGC351.0131407E-746.00000434
TAGCGAC351.0131407E-746.00000430
TAAACTA551.8189894E-1246.00000435
TCCTCAA351.0131407E-746.00000436
TGACGGG551.8189894E-1246.00000443
ATGTATT351.0131407E-746.00000441
GTAGCGA351.0131407E-746.00000429
Produced by FastQC (version 0.11.5)