FastQCFastQC Report
Fri 17 Jun 2016
SRR1527880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181803
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGAAG64993.5747484914990397No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGGAG13060.7183599830585854No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC10400.57204776598846Illumina Single End Adapter 2 (100% over 25bp)
GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9370.515393035318449No Hit
GTTGCCGGGGGGGCTGGAGAGATGGCTCATCAGTTAAGAGCACTGACTGCTC6050.33277778694520993No Hit
GTTGCCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5560.3058255364322921Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGAAGCA5500.3025252608592818No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGTGCCGGGAAG5310.2920743882114156No Hit
GTTGCCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA5190.285473837065395No Hit
GTTGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG5170.28437374520772485No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA5120.2816235155635496No Hit
GTTGCCGGGGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA4860.26732232141383805No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTC4700.25852158655247715No Hit
GTTGCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAACACAGATTGTTC4650.2557713569083019No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG4540.2497208516911162Illumina Single End Adapter 2 (100% over 23bp)
GTTGCCGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTT4380.24092011682975528Illumina Single End Adapter 2 (100% over 27bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT4200.23101929011072425Illumina Single End Adapter 2 (95% over 24bp)
GTTGCCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG4150.22826906046654896No Hit
GTTGCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4020.22111846339169322Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGCTGCCGATGCTGGCGATTCTGGTGGTACAGACCATCTTCATGG3780.20791736109965184No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATGCCGGGAAG3710.20406703959780642No Hit
GTTGCCGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGCG3620.1991166262382909No Hit
GTTGCCGGGGGATGCAGGATCAGAGAGGGTGACAATCCCGTCTGTGCCTCGG3560.19581635066528053No Hit
GTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3480.19141598323460007No Hit
GTTGCCGGGGCTGTTGCCGGGGTCTCTGCAGAAGCTGGGAGCACAGGTCCTG3360.1848154320885794No Hit
GTTGCCGGGAGCGCAGGCCACTGAGTGGGTTGGAGCCACCACTGAGTGGTCC3220.1771147890848886No Hit
GTTGCCGGGGACCCAAAGGGTAAAGTTTTTGAAATAAACTATTTTATATCTT3210.17656474315605353No Hit
GTTGCCTGGGATTCTCCCAGCTGCGCCATAGGTCATCCTCAACAACCCAGAG3080.1694141460811978No Hit
GTTGCCGGGGTACTGTATAAATGAACCAAAACTGTTAAATATGTATTTAGTT3020.16611387050818743No Hit
GTTGCCGGGGGCTGTTGCCGGGAGGCCCGGGAGGAAAAGCATCAGAAGCAGC2930.16116345714867192No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCCGTTGCCGGGGAG2840.1562130437891564No Hit
GTTGCCGGGGCTGTGCCGGGGAGGAACCTGCAGGAGGAGGCGGGGCCGGGGA2820.15511295193148628No Hit
GTTGCCGGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGA2790.15346281414498109No Hit
GTTGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC2740.15071258450080582No Hit
GTTGCCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA2740.15071258450080582Illumina Single End Adapter 2 (100% over 34bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGTAG2690.14796235485663053No Hit
GTTGCCGGCGTGGCCCTGGGAGCTGGAGCAAGAGTCCACCTTTAGCTGTGCC2660.14631221707012534No Hit
GTTGCCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA2630.14466207928362018Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT2620.14411203335478512No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT2570.14136180371060983No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGTGCCGGGGAG2490.13696143627992938No Hit
GTTGCCGGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCC2470.13586134442225925No Hit
GTTGCCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA2460.13531129849342421Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGCAA2430.13366116070691902No Hit
GTTGCCGGGGACAACTGTGATTGTCAGTCTCTATTAAAATCCCTCAGTTGCA2430.13366116070691902No Hit
GTTGCCGGGATTACTGTAGCTCCCTATAGTGTTACGTTCAGACACCGTAGCT2300.12651056363206328No Hit
GTTGCCGGGACCCTTCCCACCCCGAGCGAGCCAGCACCACACGGCACAGCTC2230.12266024213021787No Hit
GTTGCCGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCT2230.12266024213021787No Hit
GTTGCCGGGAGTTAGTTGTTATAGTTGTCTTTGTTAAGAGATTGTTCCTCTG2230.12266024213021787No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT2200.1210101043437127No Hit
GTTGCCGGGTTACCACTTTTCTTGCCTTTGTTTCTGAATTTTTTTCTGCTAA2180.11991001248604259No Hit
GTTGCCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTCTT2160.11880992062837246No Hit
GTTGCCGGGACGATCGGACCCTGCTTGTAGCTGACCCCCGTCGCTGCGAATC2120.11660973691303222No Hit
GTTGCCGGGATGCTTAAGGCAAGCATTTCACCTTGTTTTTCAGAGAACTCTA2110.11605969098419718No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGCAG2100.11550964505536213No Hit
GTTGCCGGGGAAGCAAAAGGCAGAGAGAGAGAGAGACAGAGAGAGAGGGAGA2100.11550964505536213No Hit
GTTGCCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG2070.11385950726885694No Hit
GTTGCCGGGGCGCTGTCATTTAAGAAAGAGATAGAAATTTATAAAGTTTAAG2040.11220936948235177No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT2030.11165932355351672No Hit
GTTGCCGGGGCTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACGCCA2030.11165932355351672No Hit
GTTGCCGGGAGCCGGTAGTGGTGGCGCACGGCGGTAGGATTTGCTGAAGGAG2030.11165932355351672No Hit
GTTGCCGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGG2010.11055923169584661No Hit
GTTGCCGGGAGCCCATCACTAGAAAATGACCTTGTCCTGATTTTCAGTTTTC1980.10890909390934143No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCT1980.10890909390934143Illumina Single End Adapter 2 (96% over 26bp)
GTTGCCGGCGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG1970.10835904798050637No Hit
GTTGCCGGGCTGTTGCCGGGGATAGAATGGAAGGAAGGAAGGAAGGAAGGAA1960.10780900205167132No Hit
GTTGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1960.10780900205167132No Hit
GTTGCCAAGGACATTTATAATGTAGTAACACTTCTGAAAAATAAACATAGAA1960.10780900205167132No Hit
GTTGCCGGGCTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTG1960.10780900205167132No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATG1950.10725895612283624No Hit
GTTGCCGGGAAGTCCGCTGGCCAGTCCGTCACCGACACCGTCTCCGGCGGCA1930.10615886426516616No Hit
GTTGCCGGGCTTTCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCC1910.10505877240749603No Hit
GTTGCCGGGAACACTTTCCACTTTCTGACACCTCATCCTGCTGTACGACTCC1890.10395868054982592No Hit
GTTGCCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA1870.10285858869215579Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGAGATGAACTTAGAAATCTACACAAACTGCTTTACAACAGGCCG1870.10285858869215579No Hit
GTTGCCGGGTGGCTGCCCTCCACCATATCACACATTCGAGGAACCAACCTAT1850.10175849683448568No Hit
GTTGCCGGGAGGCAGGGACCATGGCGGACGGTGGCTCGGAGCGGGCCGATGG1840.10120845090565063No Hit
GTTGCCGGGGCTGTTGCCGGGGCTTTGCCGGGCCTTCTCTGTGCTGAGCACC1840.10120845090565063No Hit
GTTGCCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATC1820.1001083590479805No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTT351.0132317E-746.00000432
GTGCATT206.297374E-446.00000442
CTTAAGG351.0132317E-746.00000413
ATTTAAT351.0132317E-746.00000438
GTGTTAC206.297374E-446.00000429
CGGGTTA351.0132317E-746.0000046
AAGTATA206.297374E-446.00000426
GTGCACC206.297374E-446.0000049
AACGTAT405.5661076E-946.00000414
AGCGTGA206.297374E-446.00000433
ATGGGAT206.297374E-446.00000446
TTAGGCG206.297374E-446.00000436
TTGGCTA206.297374E-446.00000427
CAGCGAA206.297374E-446.00000433
GGTACTG405.5661076E-946.0000049
TCGTTGC206.297374E-446.00000444
CCCCTGA206.297374E-446.00000412
ATCTCCA206.297374E-446.00000415
TCCATCG206.297374E-446.00000425
GTCGCAG206.297374E-446.00000438
Produced by FastQC (version 0.11.5)