SRR1527876_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527876_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	473038
Sequences flagged as poor quality	0
Sequence length	50
%GC	56

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGA	15530	3.28303434396391	No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3169	0.669925037734812	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGC	1699	0.359167762420778	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGG	1379	0.2915199201755462	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG	1248	0.26382658475640436	Illumina Single End Adapter 2 (100% over 23bp)
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1152	0.2435322320828348	No Hit
AGGACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	1140	0.2409954379986386	No Hit
AGGACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	1120	0.2367674478583116	No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	1104	0.23338505574604998	No Hit
AGGACTGGGGCTAGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTG	797	0.16848540709203066	No Hit
AGGACTGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	777	0.16425741695170365	No Hit
AGGACTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	744	0.15728123322016413	No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	721	0.1524190445587881	No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	719	0.15199624554475538	No Hit
AGGACTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	700	0.14797965491144474	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG	678	0.14332886575708506	No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	655	0.138466677095709	No Hit
AGGACTGGGAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTC	613	0.1295878978010223	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGC	599	0.12662830470279343	Illumina Single End Adapter 2 (100% over 21bp)
AGGACTGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCC	590	0.12472570913964627	Illumina Single End Adapter 2 (95% over 22bp)
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	584	0.12345731209754819	No Hit
AGGACTGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTC	574	0.1213433170273847	Illumina Single End Adapter 2 (100% over 25bp)
AGGACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	571	0.12070911850633563	No Hit
AGGACTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	570	0.1204977189993193	No Hit
AGGACTGGGTCGGGCCTGGGGCCTTGTCCAAGGTGCCAATGATGCGGGCC	566	0.11965212097125388	No Hit
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	558	0.1179609249151231	No Hit
AGGACTGGGGGTAATGCACTTTGGATGGGAAGAAAATGTACGTGCTTTTC	558	0.1179609249151231	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGT	518	0.10950494463446911	No Hit
AGGACTGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA	515	0.10887074611342007	Illumina Single End Adapter 2 (100% over 34bp)
AGGACTGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA	483	0.10210596188889687	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCAC	20	7.851777E-4	44.000004	18
CTAGAAA	40	8.294592E-9	44.000004	21
AACCGAA	20	7.851777E-4	44.000004	42
CGGCGCA	20	7.851777E-4	44.000004	12
CTCGTCA	20	7.851777E-4	44.000004	20
TTTACGT	20	7.851777E-4	44.000004	12
AAATACG	20	7.851777E-4	44.000004	42
CCCTATC	20	7.851777E-4	44.000004	39
TAGACGG	20	7.851777E-4	44.000004	24
ACACCGT	20	7.851777E-4	44.000004	21
CGGTTAC	20	7.851777E-4	44.000004	19
CGTTGAA	20	7.851777E-4	44.000004	22
ATTACCT	20	7.851777E-4	44.000004	42
ATTACAC	20	7.851777E-4	44.000004	19
CCAATCC	20	7.851777E-4	44.000004	40
GAATCGT	20	7.851777E-4	44.000004	13
GCAAAAC	20	7.851777E-4	44.000004	37
CGTCTAC	20	7.851777E-4	44.000004	11
TTGTACC	20	7.851777E-4	44.000004	33
AATGGGT	20	7.851777E-4	44.000004	16

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content