##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527876_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	473038
Sequences flagged as poor quality	0
Sequence length	50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.82908349857728	31.0	31.0	34.0	3.0	34.0
2	30.19693555274629	34.0	31.0	34.0	16.0	34.0
3	32.17770031160288	34.0	31.0	34.0	28.0	34.0
4	35.62403020476156	37.0	35.0	37.0	33.0	37.0
5	36.065603609012385	37.0	35.0	37.0	35.0	37.0
6	36.30082149848427	37.0	36.0	37.0	35.0	37.0
7	36.43290179647301	37.0	37.0	37.0	35.0	37.0
8	36.552970374473084	37.0	37.0	37.0	35.0	37.0
9	38.54806379191524	39.0	39.0	39.0	37.0	39.0
10	37.88964945733746	39.0	38.0	39.0	35.0	39.0
11	37.85038199890918	39.0	38.0	39.0	35.0	39.0
12	37.6450707976949	39.0	37.0	39.0	35.0	39.0
13	37.38080238796883	39.0	37.0	39.0	35.0	39.0
14	38.30413624275428	40.0	38.0	40.0	35.0	40.0
15	38.41325432629091	40.0	38.0	40.0	35.0	40.0
16	37.78848422325479	40.0	38.0	40.0	33.0	40.0
17	38.17803009483382	40.0	38.0	40.0	34.0	40.0
18	38.20873798722302	40.0	38.0	40.0	34.0	40.0
19	37.40613650488967	40.0	37.0	40.0	32.0	40.0
20	37.74128294132818	40.0	37.0	40.0	33.0	40.0
21	37.9262638519527	40.0	38.0	40.0	33.0	40.0
22	37.93146005183516	40.0	38.0	40.0	34.0	40.0
23	37.888106240936246	40.0	38.0	40.0	34.0	40.0
24	37.88946342577129	40.0	38.0	40.0	34.0	40.0
25	37.64453384294708	40.0	37.0	40.0	33.0	40.0
26	37.322058692959125	40.0	37.0	40.0	32.0	40.0
27	37.210921744130495	40.0	37.0	40.0	32.0	40.0
28	36.964148757605095	39.0	36.0	40.0	31.0	40.0
29	37.124691885218525	40.0	37.0	40.0	31.0	40.0
30	37.007561760365974	40.0	36.0	40.0	31.0	40.0
31	37.00415400031287	40.0	36.0	40.0	31.0	40.0
32	36.35464381297063	40.0	35.0	40.0	30.0	40.0
33	36.403587026835055	40.0	35.0	40.0	30.0	40.0
34	36.00295747910316	40.0	35.0	40.0	27.0	40.0
35	36.227795652780536	40.0	35.0	40.0	30.0	40.0
36	35.932419805597014	39.0	35.0	40.0	27.0	40.0
37	35.361292327466295	39.0	35.0	40.0	25.0	40.0
38	35.329685987172276	39.0	35.0	40.0	25.0	40.0
39	35.25260761291905	39.0	35.0	40.0	24.0	40.0
40	34.748618504221646	39.0	35.0	40.0	22.0	40.0
41	34.59399667679975	39.0	34.0	40.0	21.0	40.0
42	34.57219927363129	38.0	34.0	40.0	22.0	40.0
43	34.44602970585873	38.0	34.0	40.0	22.0	40.0
44	33.89925333694122	38.0	33.0	40.0	15.0	40.0
45	33.91675298813203	37.0	33.0	40.0	18.0	40.0
46	33.8248449384616	37.0	33.0	40.0	18.0	40.0
47	33.88618884740761	37.0	33.0	40.0	20.0	40.0
48	33.67478088441098	37.0	33.0	40.0	20.0	40.0
49	33.54455667409383	36.0	33.0	40.0	20.0	40.0
50	32.75081917308969	36.0	32.0	40.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	4.0
15	3.0
16	15.0
17	21.0
18	42.0
19	110.0
20	254.0
21	577.0
22	1105.0
23	1971.0
24	3095.0
25	4368.0
26	5756.0
27	7423.0
28	9530.0
29	11472.0
30	12604.0
31	13867.0
32	14916.0
33	17460.0
34	24054.0
35	30841.0
36	42552.0
37	82027.0
38	124530.0
39	64439.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.12501744045933	0.5291329660619232	0.28073854531771236	0.06511104816103569
8	98.56692274193617	0.9751859258664208	0.25346800891260324	0.20442332328481008
9	96.23962556919318	2.1793175178315485	0.8760395570757529	0.7050173558995261
10	43.853770733006655	36.37593597131731	5.6299916708594235	14.140301624816612
11	25.889252026264277	34.37081164726724	18.160697449253547	21.579238877214937
12	34.60144850942208	16.874965647580108	26.53127232907293	21.992313513924884
13	19.638168603790813	20.429436958553012	24.869249404910388	35.06314503274579
14	25.6619975562217	28.384189008071232	24.348783818636136	21.605029617070933
15	38.89116730579784	16.942402090318325	22.331821122193144	21.834609481690688
16	27.394205116713664	20.44106393143891	32.03484709473658	20.129883857110844
17	38.6643356347693	16.856785289976703	17.94697254766002	26.53190652759398
18	38.44088635585302	18.093049607008314	23.66575201146631	19.800312025672355
19	30.90301413417104	16.717473014852928	31.265564288704077	21.113948562271954
20	29.00591495820632	30.281288184035954	20.660496619721883	20.052300238035844
21	30.10286700011416	17.243646387816625	32.612179148398226	20.041307463670996
22	26.950266151979335	19.266528270456075	19.2591292877105	34.524076289854094
23	24.951906612153778	32.43143256989925	18.99340010739095	23.623260710556025
24	24.511561439038722	32.09805554733446	19.51978487986166	23.870598133765153
25	24.670745267822035	21.290889949644637	17.654818428963424	36.3835463535699
26	36.23176150753216	19.610686667878692	19.4698945962058	24.687657228383344
27	26.1378578465155	17.659892017131813	19.952308271217113	36.24994186513557
28	25.396902574423198	32.34010798286818	19.26737386848414	22.99561557422448
29	38.12991768103197	20.299426261737956	17.654607029456407	23.916049027773667
30	26.98874086225631	30.354855212477645	19.648527179634616	23.007876745631428
31	37.165724529530394	21.323445473725155	19.368422832837954	22.142407163906494
32	28.43196529665693	20.45670749495812	29.79380092085625	21.3175262875287
33	38.390784672690145	19.27900084137004	20.26856193371357	22.06165255222625
34	26.837378815232604	23.072564994778432	19.054705964425693	31.035350225563274
35	27.024890177956106	32.63860408677527	21.02769756340928	19.308808171859344
36	35.566275859444694	23.15543360152884	19.265894071935026	22.01239646709144
37	27.725045345194253	24.11328476781992	26.743094635103308	21.418575251882512
38	36.874416008861864	21.596785036297295	19.46228421395321	22.066514740887623
39	26.538037113297452	21.18540159564348	20.429859757567044	31.846701533492023
40	26.75472160798921	22.235634346500703	27.642810936964896	23.36683310854519
41	26.655363839691525	28.604044495368235	21.783450800992732	22.957140863947505
42	33.131587737137394	22.376215018666574	20.716094690067184	23.776102554128844
43	34.2746248715748	20.948211348771135	22.239650937134016	22.53751284252005
44	26.3693403066984	26.937370782051335	22.25212350804798	24.44116540320228
45	24.140555304225032	21.09280861157032	21.942846029283057	32.823790054921595
46	24.76968023710569	20.83976340167175	29.411379212663675	24.97917714855889
47	33.4975202837827	21.728275529661463	21.988719722305607	22.78548446425023
48	35.29039950278836	21.161724850857645	21.33190145400581	22.215974192348185
49	33.67868966129571	20.669163999509554	22.826707368118416	22.82543897107632
50	26.34629776043362	27.605604623730017	21.192166379868002	24.855931235968356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	20.5
6	41.0
7	32.5
8	24.0
9	56.0
10	88.0
11	355.0
12	622.0
13	656.0
14	690.0
15	925.5
16	1161.0
17	838.0
18	515.0
19	648.5
20	782.0
21	870.0
22	958.0
23	1066.0
24	1174.0
25	1447.0
26	1720.0
27	1834.5
28	1949.0
29	2492.5
30	3036.0
31	3433.0
32	3830.0
33	3987.0
34	4144.0
35	4660.0
36	5176.0
37	6096.5
38	7017.0
39	7235.5
40	7454.0
41	8017.5
42	8581.0
43	8515.5
44	8450.0
45	10427.5
46	12405.0
47	13100.0
48	13795.0
49	14270.0
50	14745.0
51	18765.5
52	22786.0
53	27872.5
54	32959.0
55	45019.0
56	57079.0
57	55523.5
58	53968.0
59	49272.5
60	44577.0
61	43506.5
62	42436.0
63	38640.0
64	34844.0
65	32281.5
66	29719.0
67	24135.5
68	18552.0
69	15728.0
70	12904.0
71	10982.0
72	9060.0
73	7488.0
74	5916.0
75	5990.0
76	6064.0
77	4318.0
78	2572.0
79	1760.0
80	948.0
81	544.0
82	140.0
83	94.0
84	48.0
85	35.0
86	22.0
87	33.0
88	44.0
89	43.5
90	43.0
91	21.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	473038.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.254508094487125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.94745740668488	12.344885611726754
2	9.351020939003773	3.039924910895108
3	3.5011054753544024	1.7072624186640395
4	1.7089348419820523	1.1111158088779338
5	0.9351020939003772	0.759981227723777
6	0.5423332032774093	0.5289215665549069
7	0.4018728053062817	0.4572571336763642
8	0.30823253999219663	0.40081346530299894
9	0.2197945116400052	0.32153865017186783
>10	3.783326830537131	16.835222540261036
>50	2.201846794121472	24.993975114050034
>100	1.0612563402262973	29.011834144402776
>500	0.026011184809468072	2.6651135849551197
>1k	0.010404473923787228	2.5391194787733755
>5k	0.0	0.0
>10k+	0.0013005592404734035	3.28303434396391
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGA	15530	3.28303434396391	No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3169	0.669925037734812	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGC	1699	0.359167762420778	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGG	1379	0.2915199201755462	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG	1248	0.26382658475640436	Illumina Single End Adapter 2 (100% over 23bp)
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1152	0.2435322320828348	No Hit
AGGACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	1140	0.2409954379986386	No Hit
AGGACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	1120	0.2367674478583116	No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	1104	0.23338505574604998	No Hit
AGGACTGGGGCTAGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTG	797	0.16848540709203066	No Hit
AGGACTGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	777	0.16425741695170365	No Hit
AGGACTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	744	0.15728123322016413	No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	721	0.1524190445587881	No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	719	0.15199624554475538	No Hit
AGGACTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	700	0.14797965491144474	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG	678	0.14332886575708506	No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	655	0.138466677095709	No Hit
AGGACTGGGAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTC	613	0.1295878978010223	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGC	599	0.12662830470279343	Illumina Single End Adapter 2 (100% over 21bp)
AGGACTGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCC	590	0.12472570913964627	Illumina Single End Adapter 2 (95% over 22bp)
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	584	0.12345731209754819	No Hit
AGGACTGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTC	574	0.1213433170273847	Illumina Single End Adapter 2 (100% over 25bp)
AGGACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	571	0.12070911850633563	No Hit
AGGACTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	570	0.1204977189993193	No Hit
AGGACTGGGTCGGGCCTGGGGCCTTGTCCAAGGTGCCAATGATGCGGGCC	566	0.11965212097125388	No Hit
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	558	0.1179609249151231	No Hit
AGGACTGGGGGTAATGCACTTTGGATGGGAAGAAAATGTACGTGCTTTTC	558	0.1179609249151231	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGT	518	0.10950494463446911	No Hit
AGGACTGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA	515	0.10887074611342007	Illumina Single End Adapter 2 (100% over 34bp)
AGGACTGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA	483	0.10210596188889687	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0314985265454361	0.0	0.0	0.0	0.0
8	0.04756488907867867	0.0	0.0	0.0	0.0
9	0.06574524668208474	0.0	0.0	0.0	0.0
10	0.17165639969727592	0.0	0.0	0.0	0.0
11	0.29067432214748073	0.0	0.0	0.0	0.0
12	0.3462723924927807	0.0	0.0	0.0	0.0
13	0.40948084509066923	0.0	0.0	0.0	0.0
14	0.553866708382836	0.0	0.0	0.0	0.0
15	0.6189777565438718	0.0	0.0	0.0	0.0
16	0.6976183731539538	0.0	0.0	0.0	0.0
17	0.7783729848341994	0.0	0.0	0.0	0.0
18	0.847077824614513	0.0	0.0	0.0	0.0
19	0.8992935028475514	0.0	0.0	0.0	0.0
20	0.9586967643191456	0.0	0.0	0.0	0.0
21	0.9925206854417615	0.0	0.0	0.0	0.0
22	1.0498099518431923	0.0	0.0	0.0	0.0
23	1.1210515857077021	0.0	0.0	0.0	0.0
24	1.2066683860493237	0.0	0.0	0.0	0.0
25	1.265437448999869	0.0	0.0	0.0	0.0
26	1.4506234171461911	0.0	0.0	0.0	0.0
27	1.555688972133317	0.0	0.0	0.0	0.0
28	1.8928711858243947	0.0	0.0	0.0	0.0
29	1.9856755694045722	0.0	0.0	0.0	0.0
30	2.167479145438633	0.0	0.0	0.0	0.0
31	2.221386019727802	0.0	0.0	0.0	0.0
32	2.3256059766868624	0.0	0.0	0.0	0.0
33	2.3995958041425847	0.0	0.0	0.0	0.0
34	2.490497592159615	0.0	0.0	0.0	0.0
35	2.8209150216261696	0.0	0.0	0.0	0.0
36	2.8989214397152026	0.0	0.0	0.0	0.0
37	2.9866522351269875	0.0	0.0	0.0	0.0
38	3.0591622660335958	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCAC	20	7.851777E-4	44.000004	18
CTAGAAA	40	8.294592E-9	44.000004	21
AACCGAA	20	7.851777E-4	44.000004	42
CGGCGCA	20	7.851777E-4	44.000004	12
CTCGTCA	20	7.851777E-4	44.000004	20
TTTACGT	20	7.851777E-4	44.000004	12
AAATACG	20	7.851777E-4	44.000004	42
CCCTATC	20	7.851777E-4	44.000004	39
TAGACGG	20	7.851777E-4	44.000004	24
ACACCGT	20	7.851777E-4	44.000004	21
CGGTTAC	20	7.851777E-4	44.000004	19
CGTTGAA	20	7.851777E-4	44.000004	22
ATTACCT	20	7.851777E-4	44.000004	42
ATTACAC	20	7.851777E-4	44.000004	19
CCAATCC	20	7.851777E-4	44.000004	40
GAATCGT	20	7.851777E-4	44.000004	13
GCAAAAC	20	7.851777E-4	44.000004	37
CGTCTAC	20	7.851777E-4	44.000004	11
TTGTACC	20	7.851777E-4	44.000004	33
AATGGGT	20	7.851777E-4	44.000004	16
>>END_MODULE