FastQCFastQC Report
Fri 17 Jun 2016
SRR1527875_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527875_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611931
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAG182392.980564802240776No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG39730.6492562069906574No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGGAG15930.2603234678419626No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC15400.2516623606256261Illumina Single End Adapter 2 (100% over 25bp)
AGGACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC15110.24692326422423444No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA15070.24626959575507695No Hit
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC15050.2459427615204982No Hit
AGGACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT14870.24300125340928963No Hit
AGGACTGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT11330.1851515938888535No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA9690.15835118665339717No Hit
AGGACTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT9400.15361209025200553No Hit
AGGACTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT9260.15132425060995439No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT9050.14789249114687766No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG9040.1477290740295883No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGCAG8640.1411923893380136No Hit
AGGACTGGGAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTG8570.14004846951698802No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA7770.12697510013383861No Hit
AGGACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC7740.12648484878197053No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC7490.12239942084973632No Hit
AGGACTGGGGGTAATGCACTTTGGATGGGAAGAAAATGTACGTGCTTTTCAG7420.12125550102871074No Hit
AGGACTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA7220.1179871586829234No Hit
AGGACTGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTT7100.11602615327545099Illumina Single End Adapter 2 (100% over 27bp)
AGGACTGGGTCGGGCCTGGGGCCTTGTCCAAGGTGCCAATGATGCGGGCCGA7020.11471881633713604No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT7010.11455539921984667Illumina Single End Adapter 2 (95% over 24bp)
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGGCTGGGAAG6970.11390173075068921No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG6750.11030655417032312Illumina Single End Adapter 2 (100% over 23bp)
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG6670.1089992172320082No Hit
AGGACTGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC6440.10524062353435273No Hit
AGGACTGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGAAGCA6220.10164544695398664No Hit
AGGACTGGGATCATATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATT6190.10115519560211855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC351.0185249E-746.00000440
TGTCACG351.0185249E-746.00000412
CCTTATA351.0185249E-746.00000431
ATTAGAA351.0185249E-746.00000418
GCTATTG750.046.00000442
TGACGTT351.0185249E-746.00000416
CGACAGT351.0185249E-746.00000439
CCGTAAA351.0185249E-746.00000428
CGCTCCA351.0185249E-746.00000438
TTGCGAC351.0185249E-746.00000446
AGTTATA351.0185249E-746.00000441
CATAAGT351.0185249E-746.00000418
TCGCGAC351.0185249E-746.00000426
GCATAAG206.309129E-446.038
CTTAACG301.8599658E-646.044
TAATATC206.309129E-446.016
AAGTACG206.309129E-446.019
AGCGTAC206.309129E-446.017
TAATAGC253.4152697E-546.021
ACTATGT206.309129E-446.027
Produced by FastQC (version 0.11.5)