FastQCFastQC Report
Fri 17 Jun 2016
SRR1527874_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527874_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617581
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAG167352.709766006402399No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG36930.5979782409109089No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGGAG22090.35768587440351957No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC14700.23802545738939507Illumina Single End Adapter 2 (100% over 25bp)
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC14510.23494893787211718No Hit
AGGACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC14250.23073896379584216No Hit
AGGACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT14250.23073896379584216No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA13510.21875672988644407No Hit
AGGACTGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT9700.1570644174610294No Hit
AGGACTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT9670.15657865122145923No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT9280.15026369010704668No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA9160.14832062514876593No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG8700.14087220947535628No Hit
AGGACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC8540.1382814561976486No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGCAG8410.13617646915951104No Hit
AGGACTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT8380.13569070291994087No Hit
AGGACTGGGAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTG8150.13196649508323605No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA7440.12047002741340812No Hit
AGGACTGGGGGTAATGCACTTTGGATGGGAAGAAAATGTACGTGCTTTTCAG7250.11739350789613022No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC7070.11447891045870905No Hit
AGGACTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA6770.10962124806300712No Hit
AGGACTGGGGCTAGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCT6470.10476358566730518No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG6450.10443974150759171Illumina Single End Adapter 2 (100% over 23bp)
AGGACTGGGATCATATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATT6340.10265859862916768No Hit
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG6330.10249667654931094No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT6320.1023347544694542Illumina Single End Adapter 2 (95% over 24bp)
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGGCTGGGAAG6240.10103937783060037No Hit
AGGACTGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTT6190.1002297674313167Illumina Single End Adapter 2 (100% over 27bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAG351.0185431E-746.00000445
CCTAGGT351.0185431E-746.00000412
CCCGTTA351.0185431E-746.00000426
AATTGCG351.0185431E-746.00000444
CCTCAAC700.046.00000443
TTGCGAC351.0185431E-746.00000446
ACATGTT351.0185431E-746.00000426
CATAAGA351.0185431E-746.00000423
GTCCCTA351.0185431E-746.00000419
GCAATTA351.0185431E-746.00000424
TAAGGCA750.046.00000421
CGCACTA351.0185431E-746.00000431
AACTATA351.0185431E-746.00000444
CGCAATT351.0185431E-746.00000423
ATTGCGA351.0185431E-746.00000445
CTGATAG351.0185431E-746.00000429
TAAGATA351.0185431E-746.00000427
GTTATGT351.0185431E-746.00000429
ATATTCG700.046.00000413
TTTAGTA206.3091755E-446.040
Produced by FastQC (version 0.11.5)