##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527874_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	617581
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.947056337549245	31.0	31.0	34.0	30.0	34.0
2	32.35102601925901	34.0	31.0	34.0	31.0	34.0
3	32.66099993361195	34.0	31.0	34.0	31.0	34.0
4	35.70922032899328	37.0	35.0	37.0	35.0	37.0
5	36.10865133480466	37.0	35.0	37.0	35.0	37.0
6	36.119887107925926	37.0	35.0	37.0	35.0	37.0
7	36.30455438234013	37.0	35.0	37.0	35.0	37.0
8	36.44120690241442	37.0	37.0	37.0	35.0	37.0
9	38.41813948291803	39.0	39.0	39.0	37.0	39.0
10	37.435960627027065	39.0	37.0	39.0	34.0	39.0
11	37.43029983111527	39.0	37.0	39.0	34.0	39.0
12	37.2467190538569	39.0	37.0	39.0	34.0	39.0
13	36.881312734685814	39.0	37.0	39.0	33.0	39.0
14	37.88900889114141	40.0	38.0	40.0	33.0	40.0
15	38.0096084562187	40.0	38.0	40.0	33.0	40.0
16	37.306963782888396	40.0	37.0	40.0	32.0	40.0
17	37.791562564262826	40.0	37.0	40.0	33.0	40.0
18	37.887051253195935	40.0	37.0	40.0	33.0	40.0
19	36.90587145653768	40.0	36.0	40.0	31.0	40.0
20	37.4351234898742	40.0	37.0	40.0	32.0	40.0
21	37.64871652463402	40.0	37.0	40.0	33.0	40.0
22	37.57907707652923	40.0	37.0	40.0	32.0	40.0
23	37.61079275431077	40.0	37.0	40.0	33.0	40.0
24	37.62493826720706	40.0	37.0	40.0	33.0	40.0
25	37.41485408391774	40.0	37.0	40.0	32.0	40.0
26	36.841957573176636	40.0	36.0	40.0	31.0	40.0
27	36.76984719413324	40.0	36.0	40.0	30.0	40.0
28	36.61182419795946	39.0	35.0	40.0	30.0	40.0
29	36.601427181211854	39.0	35.0	40.0	30.0	40.0
30	36.058167268746935	39.0	35.0	40.0	29.0	40.0
31	35.99298229705901	39.0	35.0	40.0	29.0	40.0
32	34.89305694313782	38.0	34.0	40.0	24.0	40.0
33	35.03710444459917	38.0	34.0	40.0	25.0	40.0
34	34.884575464594924	38.0	34.0	40.0	24.0	40.0
35	35.09908821676833	38.0	34.0	40.0	25.0	40.0
36	34.70041824473227	38.0	33.0	40.0	24.0	40.0
37	34.02406809794991	38.0	33.0	40.0	18.0	40.0
38	33.84446898463521	38.0	32.0	40.0	20.0	40.0
39	33.90847678280258	38.0	33.0	40.0	21.0	40.0
40	33.478672433251674	37.0	32.0	40.0	15.0	40.0
41	33.07733398533958	37.0	31.0	40.0	15.0	40.0
42	33.178213060311116	37.0	31.0	40.0	15.0	40.0
43	33.25230536561196	37.0	31.0	40.0	18.0	40.0
44	32.730879026394916	36.0	31.0	40.0	14.0	40.0
45	32.655763373549384	36.0	31.0	40.0	15.0	40.0
46	32.49268840848407	36.0	31.0	39.0	14.0	40.0
47	32.38744553346039	35.0	31.0	39.0	14.0	40.0
48	32.40783314253515	35.0	30.0	39.0	17.0	40.0
49	32.32783068131953	35.0	30.0	39.0	17.0	40.0
50	31.43306869868082	35.0	29.0	39.0	12.0	40.0
51	31.81670420560218	35.0	30.0	39.0	12.0	40.0
52	31.525433910693497	35.0	30.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	7.0
16	18.0
17	48.0
18	142.0
19	272.0
20	712.0
21	1465.0
22	2739.0
23	4553.0
24	6811.0
25	8905.0
26	11185.0
27	14137.0
28	16835.0
29	17727.0
30	18976.0
31	19836.0
32	22268.0
33	26699.0
34	36195.0
35	43028.0
36	61140.0
37	94080.0
38	158605.0
39	51195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.22892705572225	0.497262707240022	0.22426208060157293	0.04954815643615979
8	98.68260195828563	0.9397957514884687	0.21875672988644407	0.15884556033945346
9	96.26154302026778	2.230638572106331	0.7730160092360354	0.7348023983898468
10	43.44417979180059	37.38456979732213	5.47442359787623	13.696826813001048
11	25.557943006666335	35.447495955996054	17.715408990885408	21.279152046452207
12	35.54335382727124	16.62097765313376	26.159159689174377	21.676508830420623
13	19.602448909535752	20.070565642401565	24.450557902526146	35.87642754553654
14	25.356188095164846	29.305467622870523	23.989079974934462	21.34926430703017
15	39.8339650993149	16.571429496697597	22.17458114805993	21.420024255927565
16	27.006336010984793	19.96612590089397	33.09104392784105	19.93649416028019
17	39.9035915936533	16.38214258534508	17.584575950361167	26.129689870640448
18	39.658117720590496	17.480136208853576	23.38543446122857	19.47631160932736
19	30.3417689339536	16.376961078789666	32.3431258409828	20.938144146273928
20	28.438212963157874	31.118671073106203	20.582887103068263	19.860228860667668
21	29.471437754723674	16.870337656113126	33.74407567590324	19.91414891325996
22	26.710504371086547	18.81113570527591	18.98682116192046	35.49153876171708
23	24.632882164444826	33.531471985051354	18.64953746957889	23.18610838092493
24	24.11246459978529	33.06092642098769	19.140323293624643	23.686285685602375
25	24.4181734865548	20.8099018590274	17.316271064038563	37.45565359037924
26	37.32757322521256	19.16072547568659	19.075716383761808	24.43598491533904
27	26.017963635539303	17.23369080331163	19.40037015387455	37.34797540727451
28	24.97113738926554	33.352710008889524	18.840605523809835	22.835547078035106
29	39.06661636287386	20.03818122643022	17.37035303871071	23.524849371985212
30	27.055236479101524	31.256790607223994	19.19731986573421	22.49065304794027
31	38.50863287568756	21.019267108282154	19.026977837724928	21.445122178305358
32	28.987452658031902	20.047248862902194	29.74557183592112	21.219726643144785
33	39.366981821008096	18.946502564036134	20.03510470691294	21.65141090804283
34	26.76215751456084	22.54764962004984	18.78862853617582	31.901564329213492
35	26.72766811155136	33.479656919497195	20.52993858295511	19.26273638599633
36	36.83759701156609	22.842509727468947	18.821012952147168	21.498880308817792
37	28.185290674421655	23.69033373759879	26.981238088607	21.143137499372553
38	37.99096798638559	21.089865135099686	19.05320921466172	21.865957663853
39	26.359943068196724	20.80585380703098	20.015350213170418	32.81885291160188
40	26.634239071474024	21.95906285977062	28.32065753318188	23.08604053557347
41	27.153685103654418	28.632843303145663	21.768318649699392	22.445152943500528
42	34.362618020955956	22.055406497285375	20.235240397615858	23.346735084142807
43	35.613142243689495	20.620291103515168	21.754231428751854	22.012335224043486
44	26.670023851122366	27.07725788196204	21.865795741773145	24.386922525142452
45	24.042514261287184	20.847143937394446	21.84021205315578	33.27012974816259
46	24.810834530207373	20.28818891772901	29.895511681868452	25.005464870195166
47	34.87785407906007	21.099742381970948	21.652868206761543	22.369535332207434
48	36.83014859589269	20.532691258312674	20.898635158788885	21.738524987005754
49	35.33722701961362	20.07024179824185	22.256027954227868	22.336503227916662
50	27.10494655761754	26.872102606783564	21.235756929050602	24.787193906548293
51	25.63145563092129	30.169159996826327	21.695291791684006	22.50409258056838
52	33.04975379747758	23.1300833412945	21.19333334412814	22.626829517099782
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	31.0
6	62.0
7	41.0
8	20.0
9	55.0
10	90.0
11	380.0
12	670.0
13	736.5
14	1042.0
15	1281.0
16	961.0
17	641.0
18	793.5
19	946.0
20	998.0
21	1050.0
22	1123.5
23	1197.0
24	1349.0
25	1501.0
26	1748.0
27	1995.0
28	2570.5
29	3146.0
30	3612.0
31	4078.0
32	4333.0
33	4588.0
34	4752.0
35	4916.0
36	6026.0
37	7136.0
38	7862.0
39	9494.5
40	10401.0
41	10755.5
42	11110.0
43	11305.0
44	11500.0
45	13131.0
46	14762.0
47	15716.0
48	16670.0
49	18330.0
50	19990.0
51	23736.5
52	27483.0
53	34310.0
54	41137.0
55	56165.5
56	71194.0
57	66722.0
58	62250.0
59	63461.0
60	64672.0
61	58433.5
62	52195.0
63	51083.5
64	43284.0
65	36596.0
66	33525.5
67	30455.0
68	24398.5
69	18342.0
70	15171.5
71	12001.0
72	11059.5
73	10118.0
74	8901.5
75	7685.0
76	5882.0
77	4079.0
78	2764.0
79	1449.0
80	981.5
81	514.0
82	343.5
83	173.0
84	123.0
85	73.0
86	49.0
87	25.0
88	25.0
89	13.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	617581.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.07185949393327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.28540730634975	15.914055553723227
2	8.979951262966798	3.6048864002527643
3	3.299923384511451	1.9870679554397574
4	1.5425161692192733	1.2384467126275782
5	0.8343937840761854	0.8373917398294245
6	0.494864578297275	0.5959711370464467
7	0.35101245174747714	0.4931830828467476
8	0.25782828470688995	0.4140074483358816
9	0.199995914813473	0.361286091134701
>10	2.254566377312846	11.694126723601917
>50	1.5140211491347393	21.827843032407817
>100	0.9533915671209209	32.689127518070855
>500	0.025702215794329797	3.544979064512098
>1k	0.005622359705009642	2.0996774449136186
>5k	0.0	0.0
>10k+	8.031942435728062E-4	2.697950095257172
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAG	16735	2.709766006402399	No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3693	0.5979782409109089	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGGAG	2209	0.35768587440351957	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC	1470	0.23802545738939507	Illumina Single End Adapter 2 (100% over 25bp)
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1451	0.23494893787211718	No Hit
AGGACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1425	0.23073896379584216	No Hit
AGGACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1425	0.23073896379584216	No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1351	0.21875672988644407	No Hit
AGGACTGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	970	0.1570644174610294	No Hit
AGGACTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	967	0.15657865122145923	No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	928	0.15026369010704668	No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	916	0.14832062514876593	No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	870	0.14087220947535628	No Hit
AGGACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	854	0.1382814561976486	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGCAG	841	0.13617646915951104	No Hit
AGGACTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	838	0.13569070291994087	No Hit
AGGACTGGGAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTG	815	0.13196649508323605	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA	744	0.12047002741340812	No Hit
AGGACTGGGGGTAATGCACTTTGGATGGGAAGAAAATGTACGTGCTTTTCAG	725	0.11739350789613022	No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	707	0.11447891045870905	No Hit
AGGACTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA	677	0.10962124806300712	No Hit
AGGACTGGGGCTAGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCT	647	0.10476358566730518	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG	645	0.10443974150759171	Illumina Single End Adapter 2 (100% over 23bp)
AGGACTGGGATCATATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATT	634	0.10265859862916768	No Hit
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	633	0.10249667654931094	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT	632	0.1023347544694542	Illumina Single End Adapter 2 (95% over 24bp)
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGGCTGGGAAG	624	0.10103937783060037	No Hit
AGGACTGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTT	619	0.1002297674313167	Illumina Single End Adapter 2 (100% over 27bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.025583688617363553	0.0	0.0	0.0	0.0
8	0.03967090956489918	0.0	0.0	0.0	0.0
9	0.05553927339085885	0.0	0.0	0.0	0.0
10	0.13423340420123028	0.0	0.0	0.0	0.0
11	0.23122473003541236	0.0	0.0	0.0	0.0
12	0.2759152240758702	0.0	0.0	0.0	0.0
13	0.3238441597134627	0.0	0.0	0.0	0.0
14	0.44512379752615444	0.0	0.0	0.0	0.0
15	0.5000153825975864	0.0	0.0	0.0	0.0
16	0.5712610977345481	0.0	0.0	0.0	0.0
17	0.6463929427880716	0.0	0.0	0.0	0.0
18	0.7119713851300478	0.0	0.0	0.0	0.0
19	0.7676725806007633	0.0	0.0	0.0	0.0
20	0.8283933605470376	0.0	0.0	0.0	0.0
21	0.8623969973169512	0.0	0.0	0.0	0.0
22	0.9132405303919647	0.0	0.0	0.0	0.0
23	0.9804381935325083	0.0	0.0	0.0	0.0
24	1.060751545141447	0.0	0.0	0.0	0.0
25	1.1217961692474347	0.0	0.0	0.0	0.0
26	1.289871288138722	0.0	0.0	0.0	0.0
27	1.381357263257775	0.0	0.0	0.0	0.0
28	1.699696072256109	0.0	0.0	0.0	0.0
29	1.7804951901046178	0.0	0.0	0.0	0.0
30	1.935292698447653	0.0	0.0	0.0	0.0
31	1.9850027769636696	0.0	0.0	0.0	0.0
32	2.0748695312841554	0.0	0.0	0.0	0.0
33	2.1381810645081374	0.0	0.0	0.0	0.0
34	2.2139605978810875	0.0	0.0	0.0	0.0
35	2.5063918741023445	0.0	0.0	0.0	0.0
36	2.5781233554788763	0.0	0.0	0.0	0.0
37	2.653902888851827	0.0	0.0	0.0	0.0
38	2.7089563960031153	0.0	0.0	0.0	0.0
39	2.7491130718075847	0.0	0.0	0.0	0.0
40	2.797203929525034	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATAG	35	1.0185431E-7	46.000004	45
CCTAGGT	35	1.0185431E-7	46.000004	12
CCCGTTA	35	1.0185431E-7	46.000004	26
AATTGCG	35	1.0185431E-7	46.000004	44
CCTCAAC	70	0.0	46.000004	43
TTGCGAC	35	1.0185431E-7	46.000004	46
ACATGTT	35	1.0185431E-7	46.000004	26
CATAAGA	35	1.0185431E-7	46.000004	23
GTCCCTA	35	1.0185431E-7	46.000004	19
GCAATTA	35	1.0185431E-7	46.000004	24
TAAGGCA	75	0.0	46.000004	21
CGCACTA	35	1.0185431E-7	46.000004	31
AACTATA	35	1.0185431E-7	46.000004	44
CGCAATT	35	1.0185431E-7	46.000004	23
ATTGCGA	35	1.0185431E-7	46.000004	45
CTGATAG	35	1.0185431E-7	46.000004	29
TAAGATA	35	1.0185431E-7	46.000004	27
GTTATGT	35	1.0185431E-7	46.000004	29
ATATTCG	70	0.0	46.000004	13
TTTAGTA	20	6.3091755E-4	46.0	40
>>END_MODULE