FastQCFastQC Report
Fri 17 Jun 2016
SRR1527873_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527873_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2852832
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC185460.6500908570851701No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA161930.5676114120985744No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG161700.5668051956792408No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC111780.3918211797960763No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT103580.3630778118024475No Hit
CAAAGTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC101630.3562424986820114No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGA90820.31835032697333737No Hit
CAAAGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT82260.2883450550190127No Hit
CAAAGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT76620.2685752263014436No Hit
CAAAGTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC71940.25217047481239696No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66140.23183979989007414No Hit
CAAAGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG53660.188093795919283No Hit
CAAAGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT47610.16688679880203253No Hit
CAAAGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC47160.16530941885116263No Hit
CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46750.16387225045148118No Hit
CAAAGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC46720.16376709178808985No Hit
CAAAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA41470.14536432569460803Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTG41100.144067368846115No Hit
CAAAGTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA36330.12734714136689437Illumina Single End Adapter 2 (100% over 34bp)
CAAAGTGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA34750.12180878509495126No Hit
CAAAGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC34580.12121288600240043No Hit
CAAAGTGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA33540.11756738567150116Illumina Single End Adapter 2 (100% over 34bp)
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG29550.10358128344045497No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG207.858446E-444.00000434
AGTGGGG1530800.041.295274
AAGTGGG2922200.041.1971133
CAAAGTG3032550.041.1518361
AAAGTGG3000800.041.1290322
GTGGGAT207500.041.0737345
GTGGGGG539000.040.8489765
GTGGGGT361350.040.8097425
GTGGGAC98950.040.6205185
TGGGGGG251050.040.6174056
AGTGGGC261500.040.348764
GTGGGGA417350.040.109745
AGTGGGA773300.040.108114
TGGGATG61000.039.996726
GTGGGGC243700.039.8473555
TGGGATT64350.039.7264946
TGGGTAC28800.039.6458326
TGGGTAT27150.039.624316
TGGGGTT92100.039.342026
GTGGGAG208950.038.9988025
Produced by FastQC (version 0.11.5)