##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527873_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2852832
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.015967641978214	33.0	31.0	33.0	31.0	34.0
2	32.37618478760754	33.0	31.0	34.0	31.0	34.0
3	33.066079951430716	34.0	31.0	34.0	31.0	34.0
4	36.54830393097105	37.0	37.0	37.0	35.0	37.0
5	36.44976465491133	37.0	37.0	37.0	35.0	37.0
6	34.28467256396451	35.0	35.0	35.0	32.0	37.0
7	35.911479540330454	36.0	35.0	37.0	35.0	37.0
8	36.64603488743817	37.0	37.0	37.0	35.0	37.0
9	38.75546895155411	39.0	39.0	39.0	39.0	39.0
10	38.30192138899171	39.0	39.0	39.0	37.0	39.0
11	38.07616501777883	39.0	38.0	39.0	37.0	39.0
12	37.689678536976594	39.0	37.0	39.0	35.0	39.0
13	37.58383529068659	39.0	37.0	39.0	35.0	39.0
14	38.38106835593543	40.0	38.0	40.0	35.0	40.0
15	38.447468340231744	40.0	38.0	40.0	35.0	40.0
16	38.41572374398493	40.0	38.0	40.0	35.0	40.0
17	38.38473839328779	40.0	38.0	40.0	35.0	40.0
18	38.301950482888586	40.0	38.0	40.0	34.0	40.0
19	38.271820072124825	40.0	38.0	40.0	34.0	40.0
20	38.19465464492827	40.0	38.0	40.0	34.0	40.0
21	38.23188677075972	40.0	38.0	40.0	34.0	40.0
22	38.25700567015513	40.0	38.0	40.0	34.0	40.0
23	38.21860067469798	40.0	38.0	40.0	34.0	40.0
24	38.12655529663156	40.0	38.0	40.0	34.0	40.0
25	37.97075222095097	40.0	37.0	40.0	34.0	40.0
26	37.76080996006775	40.0	37.0	40.0	34.0	40.0
27	37.60976110755909	40.0	36.0	40.0	34.0	40.0
28	37.48134870893204	40.0	36.0	40.0	34.0	40.0
29	37.47113254478357	40.0	36.0	40.0	34.0	40.0
30	37.54743847517134	40.0	36.0	40.0	34.0	40.0
31	37.53856974402979	40.0	36.0	40.0	34.0	40.0
32	37.45705425345761	40.0	36.0	40.0	34.0	40.0
33	37.33491947650615	40.0	35.0	40.0	33.0	40.0
34	36.986288712409284	40.0	35.0	40.0	33.0	40.0
35	36.91271270092315	40.0	35.0	40.0	32.0	40.0
36	36.8250913478256	40.0	35.0	40.0	32.0	40.0
37	36.62296973673879	40.0	35.0	40.0	31.0	40.0
38	36.44337591558143	40.0	35.0	40.0	31.0	40.0
39	36.235691761730095	40.0	35.0	40.0	30.0	40.0
40	35.99251059999327	40.0	35.0	40.0	30.0	40.0
41	35.80259615708181	40.0	35.0	40.0	28.0	40.0
42	35.69613317573555	39.0	35.0	40.0	28.0	40.0
43	35.60128987616516	39.0	35.0	40.0	27.0	40.0
44	35.30925305100335	39.0	35.0	40.0	25.0	40.0
45	35.24582450000561	39.0	35.0	40.0	25.0	40.0
46	35.09978260198988	39.0	35.0	40.0	24.0	40.0
47	35.028450325851644	38.0	35.0	40.0	24.0	40.0
48	34.942137497055555	38.0	35.0	40.0	24.0	40.0
49	34.85806910466512	38.0	35.0	40.0	24.0	40.0
50	34.33174894280491	37.0	34.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	3.0
14	7.0
15	22.0
16	55.0
17	184.0
18	381.0
19	841.0
20	1604.0
21	2618.0
22	4347.0
23	7242.0
24	11001.0
25	15915.0
26	21985.0
27	30414.0
28	41249.0
29	50788.0
30	54709.0
31	54394.0
32	59176.0
33	75149.0
34	159852.0
35	169363.0
36	214670.0
37	356365.0
38	990316.0
39	530179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.44749638254197	0.3741895772341308	0.1164456932619937	0.06186834696189611
8	98.78461122141087	0.8857864746329263	0.18227501654496306	0.1473272874112461
9	97.23797265313905	1.3100666285291247	0.7096106605646599	0.7423500577671591
10	51.6764043588967	26.248618916220796	13.098422900472231	8.97655382441027
11	31.173549651714506	30.50666846137452	23.786328812912924	14.533453073998048
12	29.052359199560296	25.878530526858924	23.41897454879923	21.65013572478155
13	23.746999472804568	25.5745518838824	25.633055153615775	25.045393489697254
14	22.33089785868919	28.227529696806542	26.06841202005586	23.373160424448407
15	25.219325918946506	26.475866787809448	24.71985732072551	23.584949972518533
16	27.80693009612904	27.457382699016275	23.84795178966024	20.887735415194445
17	29.043420713172036	27.793680104541735	22.174702190665275	20.98819699162096
18	27.18894768426602	28.53722196049399	22.55793541295106	21.715894942288926
19	27.0497877197115	29.14489882334466	22.403492389317	21.401821067626837
20	26.007455048176688	30.170651478951445	21.81232543661877	22.0095680362531
21	25.847578826934075	28.44352559141232	25.27565590963646	20.43323967201714
22	24.284886036051194	26.925385020919563	24.94324937465648	23.84647956837276
23	25.081673228567265	29.244904712229815	23.989565456360555	21.68385660284237
24	25.996588652959584	29.049590021424326	23.452450056645464	21.501371268970622
25	25.851049062825993	28.461507722852243	22.03424526926226	23.653197945059507
26	24.90349939989456	27.86448693789189	23.30347528350776	23.92853837870579
27	23.115311381812877	27.122452356114906	23.46825890904196	26.29397735303025
28	25.759981660329107	29.453224024408026	21.399121995266455	23.387672319996412
29	27.317661888257	28.17687827393972	22.412746351695436	22.09271348610784
30	27.238687732050117	27.51963662774394	22.664811667844447	22.5768639723615
31	28.741089555921974	27.092867718814144	22.624290529550986	21.541752195712892
32	29.079595293378652	27.110569427151688	22.546718488855984	21.26311679061368
33	29.04457745846934	27.21502703278707	23.132382138170072	20.608013370573524
34	25.719740945138025	28.298406635932295	24.996144182342316	20.985708236587364
35	24.010141501497458	31.36854886652982	24.156732678264966	20.464576953707756
36	24.748530582943545	31.362589875604314	22.407663682964856	21.481215858487285
37	26.23961032405694	30.4754363383473	21.651152258527667	21.6338010790681
38	27.225262476023826	30.147726890332134	21.994074659846778	20.632935973797263
39	26.72547840181265	27.573968603829456	21.79970639701181	23.900846597346074
40	25.94723418694126	26.897062287579498	22.992310798532824	24.163392726946416
41	24.046526399030856	28.120898812127738	22.168182353535013	25.66439243530639
42	23.123969445098766	28.51471800652825	23.082256508620205	25.279056039752778
43	23.58354785700665	27.347877477538105	23.815878397325886	25.252696268129355
44	24.734859956702675	26.475551311819274	23.571770086706824	25.217818644771228
45	25.67567946517706	27.195537627171877	22.71784668708147	24.410936220569596
46	24.325091698354477	28.1225462978542	23.357386624939707	24.19497537885161
47	24.16893108321836	28.125560846204756	23.633568327893126	24.071939742683764
48	25.416708730132022	27.40238471806261	24.77127990712387	22.409626644681495
49	26.36422334017566	26.455185584009154	23.906209689179033	23.274381386636158
50	23.587578939103317	27.15729492658523	24.742711803569225	24.512414330742224
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	65.0
4	130.0
5	164.0
6	198.0
7	720.5
8	1243.0
9	12028.0
10	22813.0
11	19219.0
12	15625.0
13	14818.5
14	14012.0
15	12998.5
16	11985.0
17	12867.5
18	13750.0
19	14610.5
20	15471.0
21	17022.5
22	18574.0
23	22099.0
24	25624.0
25	29793.0
26	33962.0
27	37840.0
28	41718.0
29	44237.0
30	46756.0
31	51662.5
32	56569.0
33	59600.0
34	62631.0
35	68132.5
36	73634.0
37	82599.5
38	91565.0
39	91412.0
40	91259.0
41	100657.5
42	110056.0
43	112426.0
44	114796.0
45	120136.5
46	125477.0
47	135625.0
48	145773.0
49	160169.0
50	174565.0
51	174957.5
52	175350.0
53	181347.5
54	187345.0
55	201632.5
56	215920.0
57	216622.0
58	217324.0
59	210565.0
60	203806.0
61	198183.0
62	192560.0
63	157503.0
64	122446.0
65	101157.0
66	79868.0
67	65225.0
68	50582.0
69	43910.0
70	37238.0
71	31174.0
72	25110.0
73	23626.5
74	22143.0
75	16026.0
76	9909.0
77	6216.5
78	2524.0
79	2140.5
80	1757.0
81	1140.0
82	523.0
83	351.0
84	179.0
85	106.5
86	34.0
87	23.5
88	13.0
89	10.5
90	8.0
91	7.5
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2852832.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.558246677000346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.79270012030146	9.141486843956585
2	9.455479523764787	2.3748848861752787
3	3.5300863294306226	1.3299508474828938
4	1.6837765717930484	0.8458112615012434
5	0.930743799534689	0.5844255113822593
6	0.6170660320202782	0.46495604676650687
7	0.4319525218579182	0.37971964255708845
8	0.3582561967024569	0.3599255753402729
9	0.29740319403703747	0.3361376405920423
>10	5.9190026238419895	20.985612699010275
>50	2.611393470486837	22.551580545163585
>100	1.3156063277787715	28.5586887307688
>500	0.03465859894929413	3.0157915905048878
>1k	0.018465646981181298	4.548981904255273
>5k	0.0017045212598013509	1.5748959443908552
>10k+	0.0017045212598013509	2.9471503301522235
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	18546	0.6500908570851701	No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16193	0.5676114120985744	No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	16170	0.5668051956792408	No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	11178	0.3918211797960763	No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	10358	0.3630778118024475	No Hit
CAAAGTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	10163	0.3562424986820114	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGA	9082	0.31835032697333737	No Hit
CAAAGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	8226	0.2883450550190127	No Hit
CAAAGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	7662	0.2685752263014436	No Hit
CAAAGTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC	7194	0.25217047481239696	No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6614	0.23183979989007414	No Hit
CAAAGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	5366	0.188093795919283	No Hit
CAAAGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4761	0.16688679880203253	No Hit
CAAAGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	4716	0.16530941885116263	No Hit
CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4675	0.16387225045148118	No Hit
CAAAGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4672	0.16376709178808985	No Hit
CAAAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	4147	0.14536432569460803	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTG	4110	0.144067368846115	No Hit
CAAAGTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	3633	0.12734714136689437	Illumina Single End Adapter 2 (100% over 34bp)
CAAAGTGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA	3475	0.12180878509495126	No Hit
CAAAGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	3458	0.12121288600240043	No Hit
CAAAGTGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	3354	0.11756738567150116	Illumina Single End Adapter 2 (100% over 34bp)
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	2955	0.10358128344045497	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.04669044654574823	0.0	0.0	0.0	0.0
8	0.20267579724288007	0.0	0.0	0.0	0.0
9	0.2248292223306525	0.0	0.0	0.0	0.0
10	0.3848456551244518	0.0	0.0	0.0	0.0
11	0.5412165875873518	0.0	0.0	0.0	0.0
12	0.6149328106246705	0.0	0.0	0.0	0.0
13	0.6654790748281006	0.0	0.0	0.0	0.0
14	0.7057548429069781	0.0	0.0	0.0	0.0
15	0.74876473623403	0.0	0.0	0.0	0.0
16	0.8014842794808807	0.0	0.0	0.0	0.0
17	0.8477540913730637	0.0	0.0	0.0	0.0
18	0.8891164989736514	0.0	0.0	0.0	0.0
19	0.9517910623548811	0.0	0.0	0.0	0.0
20	1.0037744949579925	0.0	0.0	0.0	0.0
21	1.0517618983522339	0.0	0.0	0.0	0.0
22	1.1086527352469406	0.0	0.0	0.0	0.0
23	1.1746222700810984	0.0	0.0	0.0	0.0
24	1.245779632309228	0.0	0.0	0.0	0.0
25	1.3147637154939373	0.0	0.0	0.0	0.0
26	1.3905130060234883	0.0	0.0	0.0	0.0
27	1.4523463000975871	0.0	0.0	0.0	0.0
28	1.5388217742930534	0.0	0.0	0.0	0.0
29	1.58722981234086	0.0	0.0	0.0	0.0
30	1.6414566297629865	0.0	0.0	0.0	0.0
31	1.6797694361252258	0.0	0.0	0.0	0.0
32	1.7194493051115523	0.0	0.0	0.0	0.0
33	1.7541166111428923	0.0	0.0	0.0	0.0
34	1.7920438357393635	0.0	0.0	0.0	0.0
35	1.8472170811320119	0.0	0.0	0.0	0.0
36	1.877993516617873	0.0	0.0	0.0	0.0
37	1.9109432311471548	0.0	0.0	0.0	0.0
38	1.9407381857746968	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAACG	20	7.858446E-4	44.000004	34
AGTGGGG	153080	0.0	41.29527	4
AAGTGGG	292220	0.0	41.197113	3
CAAAGTG	303255	0.0	41.151836	1
AAAGTGG	300080	0.0	41.129032	2
GTGGGAT	20750	0.0	41.073734	5
GTGGGGG	53900	0.0	40.848976	5
GTGGGGT	36135	0.0	40.809742	5
GTGGGAC	9895	0.0	40.620518	5
TGGGGGG	25105	0.0	40.617405	6
AGTGGGC	26150	0.0	40.34876	4
GTGGGGA	41735	0.0	40.10974	5
AGTGGGA	77330	0.0	40.10811	4
TGGGATG	6100	0.0	39.99672	6
GTGGGGC	24370	0.0	39.847355	5
TGGGATT	6435	0.0	39.726494	6
TGGGTAC	2880	0.0	39.645832	6
TGGGTAT	2715	0.0	39.62431	6
TGGGGTT	9210	0.0	39.34202	6
GTGGGAG	20895	0.0	38.998802	5
>>END_MODULE