FastQCFastQC Report
Fri 17 Jun 2016
SRR1527872_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527872_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4227117
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC281380.665654629384519No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA216260.5116016424433012No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT177370.4196004037740143No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA166040.3927972658433632No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC155680.3682888361027149No Hit
CAAAGTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT149530.353739913042388No Hit
CAAAGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG128570.30415529070995667No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAG118760.2809479841698254No Hit
CAAAGTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT109490.25901814404474727No Hit
CAAAGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC107810.2550438040868043No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86990.20579037675086828No Hit
CAAAGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC80000.18925428371156985No Hit
CAAAGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT73910.1748473013640266No Hit
CAAAGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC70750.1673717571574196No Hit
CAAAGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT69550.16453294290174603No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT66720.15783807261544924No Hit
CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61690.1459387095270843No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT61300.1450160948939904No Hit
CAAAGTGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC53090.12559387402809055No Hit
CAAAGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC51830.1226131190596333No Hit
CAAAGTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT43750.10349843640476476No Hit
CAAAGTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT43240.10229194034610349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGGG2264300.043.063424
AAGTGGG4340300.042.9704633
CAAAGTG4505350.042.9609681
GTGGGAT303450.042.922725
AAAGTGG4460250.042.91582
GTGGGGT532550.042.8515625
GTGGGGG793750.042.4677775
TGGGTAC39950.042.315396
TGGGGGG370600.042.0590936
GTGGGAC153350.041.9954385
AGTGGGA1150200.041.96874
AGTGGGC392600.041.852274
GTGGGGA615900.041.6307835
GTGGGGC368450.041.6303445
TGGGATT100900.041.1902856
TGGGGTG203600.040.9616936
TGGGTAT42900.040.9603736
GTGGGTG222850.040.9531065
TGGGATA50550.040.9040576
TGGGGTC112850.040.8232156
Produced by FastQC (version 0.11.5)