##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527872_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4227117
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.784643292343222	31.0	31.0	33.0	30.0	34.0
2	31.988502329128814	31.0	31.0	34.0	31.0	34.0
3	32.771065243758336	34.0	31.0	34.0	31.0	34.0
4	36.4171060323147	37.0	37.0	37.0	35.0	37.0
5	36.20838032162346	37.0	37.0	37.0	35.0	37.0
6	33.907453235857915	35.0	33.0	35.0	32.0	36.0
7	35.796840257792724	36.0	35.0	37.0	35.0	37.0
8	36.56707822376338	37.0	37.0	37.0	35.0	37.0
9	38.69078901766854	39.0	39.0	39.0	38.0	39.0
10	38.06391188131296	39.0	38.0	39.0	37.0	39.0
11	37.71860490258491	39.0	37.0	39.0	35.0	39.0
12	37.36276521326474	39.0	37.0	39.0	35.0	39.0
13	37.2766199752692	39.0	37.0	39.0	34.0	39.0
14	38.07974252900973	40.0	38.0	40.0	34.0	40.0
15	38.15419303511116	40.0	38.0	40.0	34.0	40.0
16	38.12115467823578	40.0	38.0	40.0	34.0	40.0
17	38.092662918958716	40.0	38.0	40.0	34.0	40.0
18	38.07063632258109	40.0	38.0	40.0	34.0	40.0
19	38.033480029059994	40.0	38.0	40.0	34.0	40.0
20	38.03587243977396	40.0	38.0	40.0	34.0	40.0
21	38.04515323327932	40.0	38.0	40.0	34.0	40.0
22	38.04974241309147	40.0	38.0	40.0	34.0	40.0
23	38.02956009970862	40.0	38.0	40.0	34.0	40.0
24	37.937220332439345	40.0	37.0	40.0	34.0	40.0
25	37.77968956146707	40.0	37.0	40.0	34.0	40.0
26	37.54209476577062	40.0	36.0	40.0	34.0	40.0
27	37.390471803832256	40.0	35.0	40.0	33.0	40.0
28	37.305173242188474	40.0	35.0	40.0	33.0	40.0
29	37.19394589740478	40.0	35.0	40.0	33.0	40.0
30	37.03775149824337	39.0	35.0	40.0	33.0	40.0
31	36.91937057810323	39.0	35.0	40.0	32.0	40.0
32	36.72893298198276	39.0	35.0	40.0	32.0	40.0
33	36.61144179354392	39.0	35.0	40.0	31.0	40.0
34	36.37906189963514	39.0	35.0	40.0	30.0	40.0
35	36.23829409027476	39.0	35.0	40.0	30.0	40.0
36	36.19103824190341	39.0	35.0	40.0	30.0	40.0
37	35.99681011904804	39.0	35.0	40.0	30.0	40.0
38	35.83338265773103	39.0	35.0	40.0	30.0	40.0
39	35.637635769248874	39.0	35.0	40.0	29.0	40.0
40	35.376784697466384	38.0	35.0	40.0	27.0	40.0
41	35.16714820053478	38.0	35.0	40.0	26.0	40.0
42	35.07455223027893	38.0	35.0	40.0	26.0	40.0
43	34.977454373749296	38.0	34.0	40.0	26.0	40.0
44	34.821123238367896	38.0	34.0	40.0	25.0	40.0
45	34.65996493591258	38.0	34.0	40.0	24.0	40.0
46	34.49234288996496	38.0	34.0	40.0	23.0	40.0
47	34.351961159343354	37.0	34.0	40.0	23.0	40.0
48	34.19658102673761	37.0	33.0	40.0	23.0	40.0
49	34.45963833033247	37.0	34.0	40.0	24.0	40.0
50	34.41885142994622	37.0	34.0	40.0	24.0	40.0
51	34.388228194298854	37.0	34.0	40.0	24.0	40.0
52	33.919916340143885	36.0	34.0	40.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	12.0
15	34.0
16	102.0
17	336.0
18	773.0
19	1656.0
20	3056.0
21	5653.0
22	9691.0
23	15377.0
24	23092.0
25	32492.0
26	44716.0
27	59299.0
28	72234.0
29	78027.0
30	80212.0
31	90448.0
32	115197.0
33	150096.0
34	280843.0
35	281416.0
36	393757.0
37	603980.0
38	1385206.0
39	499408.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.50299459418795	0.3478020598909375	0.09737132896960268	0.05183201695150619
8	98.82943859845848	0.8577477273517625	0.1764796195610389	0.1363340546287221
9	97.23165930822356	1.3057126168970483	0.7043098168326072	0.7583182580467964
10	51.701999258596345	26.299887133476552	13.04217508055727	8.955938527369836
11	31.173965612969784	30.369587593624686	23.77343707306895	14.683009720336578
12	29.239857803793935	25.439584473294687	23.42575329710533	21.894804425806054
13	23.78668487292876	25.12949132943327	25.744922603277836	25.338901194360126
14	22.376906056775812	27.92943748658956	26.090335327836918	23.603321128797713
15	25.453849514929445	25.94967208146829	24.876765890321938	23.719712513280328
16	27.95510509881794	26.929228597173914	24.060914330026826	21.05475197398132
17	29.27725918161243	27.25706433013328	22.311873553535424	21.15380293471886
18	27.49152673086645	27.99238819270912	22.64865155140016	21.867433525024264
19	27.130713438970343	28.62154040212277	22.65260223457264	21.595143924334245
20	26.08435016111454	29.79096627796202	21.991607991924518	22.13307556899892
21	26.025563049236634	28.04237498039444	25.339658211494974	20.592403758873957
22	24.410348708114775	26.514950970129288	25.050004530274418	24.024695791481523
23	25.228140124817934	28.8575404939111	24.10872469344946	21.80559468782151
24	26.155249547149985	28.584233651446127	23.5726855916219	21.687831209781987
25	25.99942230129897	27.868947086158247	22.0906352958766	24.04099531666618
26	25.080427156381052	27.29995408217941	23.38035592580002	24.239262835639515
27	23.192994184925563	26.67058896169659	23.542783414795473	26.59363343858237
28	25.834368909117018	29.15367613434878	21.462405701096042	23.549549255438162
29	27.42987714794741	27.872684858261554	22.477683962852225	22.21975403093882
30	27.33610165036832	27.25848373726112	22.73315358907738	22.67226102329318
31	28.975800764445363	26.656181979349046	22.71060867253024	21.65740858367535
32	29.356107247563767	26.604491903110322	22.645718110002633	21.39368273932328
33	29.39017301863185	26.691264992192075	23.233470944854375	20.685091044321695
34	25.752421804269908	27.827287486956244	25.099967661174272	21.320323047599583
35	24.147072342686513	31.086317222825866	24.22071591583578	20.54589451865184
36	24.982393437418455	31.044633966838393	22.44200953037259	21.53096306537056
37	26.345639356563822	30.188376616970857	21.74827429664237	21.71770972982295
38	27.328555135805328	29.84958779234168	22.071662553934512	20.750194517918477
39	26.796443060364783	27.354766854099378	21.785297165893443	24.063492919642396
40	25.927552987059503	26.70011262995559	23.05744080421715	24.31489357876775
41	23.999548628533347	27.979447931060342	22.20695571000282	25.814047730403487
42	23.14842480111149	28.24367056790716	23.121668976751767	25.48623565422958
43	23.63975257841219	27.06875631784027	23.861061806427404	25.430429297320135
44	24.6796812106218	26.207507386239843	23.691679222505552	25.421132180632807
45	25.862544140604577	26.84780194160701	22.804668051534886	24.484985866253524
46	24.326982196139827	27.803228536139407	23.552908518974043	24.316880748746723
47	24.312149391653932	27.750781442765838	23.71266279121207	24.224406374368158
48	25.74679148932949	26.886267874771384	24.85374783806552	22.513192797833607
49	26.58277497405442	26.026911486008075	24.037777993843086	23.35253554609442
50	23.67116878950831	26.88184405588963	24.809769873888044	24.63721728071402
51	22.949305637861457	27.907058167540665	24.858952330867588	24.28468386373029
52	23.724089018591158	26.595644265346806	25.67901479897528	24.00125191708675
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	102.5
4	205.0
5	252.0
6	299.0
7	1006.5
8	1714.0
9	16090.0
10	30466.0
11	25249.5
12	20033.0
13	19390.0
14	16931.0
15	15115.0
16	15853.0
17	16591.0
18	17811.0
19	19031.0
20	19259.5
21	19488.0
22	23933.5
23	28379.0
24	32717.0
25	37055.0
26	44300.5
27	51546.0
28	56117.0
29	60688.0
30	67217.5
31	73747.0
32	77683.0
33	81619.0
34	89893.5
35	98168.0
36	106475.5
37	114783.0
38	122411.0
39	136408.0
40	142777.0
41	149443.5
42	156110.0
43	161802.5
44	167495.0
45	176849.5
46	186204.0
47	192900.0
48	199596.0
49	218741.5
50	237887.0
51	251575.5
52	265264.0
53	267843.0
54	270422.0
55	293476.0
56	316530.0
57	316092.5
58	315655.0
59	304961.5
60	294268.0
61	284714.5
62	275161.0
63	231162.0
64	162530.5
65	137898.0
66	110746.5
67	83595.0
68	72690.5
69	61786.0
70	53091.5
71	44397.0
72	41310.0
73	38223.0
74	28331.5
75	18440.0
76	12191.0
77	5942.0
78	4515.5
79	3089.0
80	1996.0
81	903.0
82	684.5
83	466.0
84	289.0
85	112.0
86	64.0
87	16.0
88	8.5
89	2.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	4227117.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.410858684443612
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.52603312891569	9.373429241595792
2	9.005223031801474	2.2352510093916993
3	3.738218286337188	1.3918349665000143
4	1.8539633730382155	0.9203710971564684
5	1.0020530819227687	0.6218169597027339
6	0.6396098892836737	0.4762864769443379
7	0.4077027527892674	0.3541958875088372
8	0.29340463439937586	0.2913122763913198
9	0.2394613029702963	0.26747283524013765
>10	3.1537793812755046	10.576347585158677
>50	2.3937091364790346	21.426788249922556
>100	1.6650394775699393	37.27498296135452
>500	0.0562511523291292	4.595248746018537
>1k	0.02173773344244315	4.783079896449069
>5k	0.0019068187230213292	1.5993691116979396
>10k+	0.0019068187230213292	3.812212698967335
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	28138	0.665654629384519	No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21626	0.5116016424433012	No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	17737	0.4196004037740143	No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	16604	0.3927972658433632	No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	15568	0.3682888361027149	No Hit
CAAAGTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	14953	0.353739913042388	No Hit
CAAAGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	12857	0.30415529070995667	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAG	11876	0.2809479841698254	No Hit
CAAAGTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	10949	0.25901814404474727	No Hit
CAAAGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	10781	0.2550438040868043	No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8699	0.20579037675086828	No Hit
CAAAGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	8000	0.18925428371156985	No Hit
CAAAGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	7391	0.1748473013640266	No Hit
CAAAGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	7075	0.1673717571574196	No Hit
CAAAGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6955	0.16453294290174603	No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	6672	0.15783807261544924	No Hit
CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6169	0.1459387095270843	No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT	6130	0.1450160948939904	No Hit
CAAAGTGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC	5309	0.12559387402809055	No Hit
CAAAGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5183	0.1226131190596333	No Hit
CAAAGTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	4375	0.10349843640476476	No Hit
CAAAGTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT	4324	0.10229194034610349	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.039814369935821506	0.0	0.0	0.0	0.0
8	0.1772129799104212	0.0	0.0	0.0	0.0
9	0.19774706969312655	0.0	0.0	0.0	0.0
10	0.34096524889185703	0.0	0.0	0.0	0.0
11	0.48165215204594525	0.0	0.0	0.0	0.0
12	0.545549129584064	0.0	0.0	0.0	0.0
13	0.5911357551730885	0.0	0.0	0.0	0.0
14	0.6291995229845779	0.0	0.0	0.0	0.0
15	0.6676181425780265	0.0	0.0	0.0	0.0
16	0.7161618663500442	0.0	0.0	0.0	0.0
17	0.7592881862508182	0.0	0.0	0.0	0.0
18	0.7989133019029282	0.0	0.0	0.0	0.0
19	0.8554530191617596	0.0	0.0	0.0	0.0
20	0.9023880815222289	0.0	0.0	0.0	0.0
21	0.9479510503257894	0.0	0.0	0.0	0.0
22	0.9948387991153308	0.0	0.0	0.0	0.0
23	1.0499827660317895	0.0	0.0	0.0	0.0
24	1.1097161493282537	0.0	0.0	0.0	0.0
25	1.1634880226878035	0.0	0.0	0.0	0.0
26	1.2230558084860201	0.0	0.0	0.0	0.0
27	1.2729479690294827	0.0	0.0	0.0	0.0
28	1.3371997983495607	0.0	0.0	0.0	0.0
29	1.3712419126321793	0.0	0.0	0.0	0.0
30	1.4141316646783137	0.0	0.0	0.0	0.0
31	1.4443177229303092	0.0	0.0	0.0	0.0
32	1.4785490914966395	0.0	0.0	0.0	0.0
33	1.5057307379947136	0.0	0.0	0.0	0.0
34	1.5404115854848588	0.0	0.0	0.0	0.0
35	1.5912973310178071	0.0	0.0	0.0	0.0
36	1.620016668571038	0.0	0.0	0.0	0.0
37	1.6487596629097325	0.0	0.0	0.0	0.0
38	1.675349889771208	0.0	0.0	0.0	0.0
39	1.7005916798612388	0.0	0.0	0.0	0.0
40	1.7246742874635361	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGGGG	226430	0.0	43.06342	4
AAGTGGG	434030	0.0	42.970463	3
CAAAGTG	450535	0.0	42.960968	1
GTGGGAT	30345	0.0	42.92272	5
AAAGTGG	446025	0.0	42.9158	2
GTGGGGT	53255	0.0	42.851562	5
GTGGGGG	79375	0.0	42.467777	5
TGGGTAC	3995	0.0	42.31539	6
TGGGGGG	37060	0.0	42.059093	6
GTGGGAC	15335	0.0	41.995438	5
AGTGGGA	115020	0.0	41.9687	4
AGTGGGC	39260	0.0	41.85227	4
GTGGGGA	61590	0.0	41.630783	5
GTGGGGC	36845	0.0	41.630344	5
TGGGATT	10090	0.0	41.190285	6
TGGGGTG	20360	0.0	40.961693	6
TGGGTAT	4290	0.0	40.960373	6
GTGGGTG	22285	0.0	40.953106	5
TGGGATA	5055	0.0	40.904057	6
TGGGGTC	11285	0.0	40.823215	6
>>END_MODULE