FastQCFastQC Report
Fri 17 Jun 2016
SRR1527871_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527871_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4246218
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC264770.6235431153087289No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA199590.4700418113248072No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT169500.39917875153842786No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA157540.3710125104269258No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC146830.3457900654182145No Hit
CAAAGTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT141110.33231925445184396No Hit
CAAAGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG120820.28453555611134423No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAG110210.2595486147908562No Hit
CAAAGTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT105780.24911580140256576No Hit
CAAAGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC102370.24108512563415255No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82660.19466734868534777No Hit
CAAAGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC74450.17533249588221803No Hit
CAAAGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT70300.16555909282095269No Hit
CAAAGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT68610.16157908048997954No Hit
CAAAGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC67470.1588943384442344No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT63940.15058105824995324No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT59250.1395359352722823No Hit
CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57680.13583852736717708No Hit
CAAAGTGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC52110.12272097193314145No Hit
CAAAGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC49780.11723373599753946No Hit
CAAAGTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT42860.10093688077248977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAT206.313387E-446.013
AGTGGGG2276000.043.1068084
AAGTGGG4352600.043.0651553
CAAAGTG4516400.043.0351141
AAAGTGG4472300.043.0063972
GTGGGAT309400.042.9893345
GTGGGGT536650.042.6013265
GTGGGGG788700.042.5763935
AGTGGGC385850.042.3459854
TGGGGGG366450.042.1462676
AGTGGGA1157900.042.035244
GTGGGAC151950.041.988815
GTGGGGC371100.041.7793055
GTGGGGA627950.041.6670075
GTGGGTG226400.041.174475
TGGGATG91500.040.9978146
TGGGATT99700.040.993986
TGGGTAT42900.040.8531466
TGGGTAC41450.040.8395656
GTGGGAG308100.040.781895
Produced by FastQC (version 0.11.5)