##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527871_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4246218
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.745146386737563	31.0	31.0	33.0	30.0	33.0
2	31.95887187139238	31.0	31.0	34.0	31.0	34.0
3	32.75688765861762	34.0	31.0	34.0	31.0	34.0
4	36.398557021801516	37.0	37.0	37.0	35.0	37.0
5	36.215334445852754	37.0	37.0	37.0	35.0	37.0
6	33.80345945497852	35.0	33.0	35.0	32.0	36.0
7	35.74114706310416	36.0	35.0	37.0	35.0	37.0
8	36.53984039444042	37.0	37.0	37.0	35.0	37.0
9	38.65312073002375	39.0	39.0	39.0	38.0	39.0
10	37.983647565904526	39.0	38.0	39.0	35.0	39.0
11	37.69849616764848	39.0	37.0	39.0	35.0	39.0
12	37.32019528907842	39.0	37.0	39.0	35.0	39.0
13	37.09407642283086	39.0	37.0	39.0	34.0	39.0
14	38.02511717486008	40.0	38.0	40.0	34.0	40.0
15	38.11838487802557	40.0	38.0	40.0	34.0	40.0
16	38.11181573814628	40.0	38.0	40.0	34.0	40.0
17	38.11498444027132	40.0	38.0	40.0	34.0	40.0
18	38.10241890548248	40.0	38.0	40.0	34.0	40.0
19	38.02245409915365	40.0	38.0	40.0	34.0	40.0
20	38.03731885645061	40.0	38.0	40.0	34.0	40.0
21	38.10042324722848	40.0	38.0	40.0	34.0	40.0
22	37.99780133756675	40.0	38.0	40.0	34.0	40.0
23	38.004785670448385	40.0	38.0	40.0	34.0	40.0
24	37.95962595420207	40.0	38.0	40.0	34.0	40.0
25	37.772176558057076	40.0	37.0	40.0	34.0	40.0
26	37.48985591413347	40.0	36.0	40.0	34.0	40.0
27	37.342209938349846	40.0	35.0	40.0	33.0	40.0
28	37.27558429642566	40.0	35.0	40.0	33.0	40.0
29	37.16116906856878	40.0	35.0	40.0	33.0	40.0
30	36.992332706422516	39.0	35.0	40.0	32.0	40.0
31	36.895577429138115	39.0	35.0	40.0	32.0	40.0
32	36.71101554371443	39.0	35.0	40.0	32.0	40.0
33	36.4181488091285	39.0	35.0	40.0	31.0	40.0
34	36.295295484122576	39.0	35.0	40.0	30.0	40.0
35	36.229637055845934	39.0	35.0	40.0	30.0	40.0
36	36.038459165308986	39.0	35.0	40.0	30.0	40.0
37	35.92266247281699	39.0	35.0	40.0	30.0	40.0
38	35.499878715600566	38.0	35.0	40.0	27.0	40.0
39	35.47604597785606	38.0	35.0	40.0	27.0	40.0
40	35.2905456102348	38.0	35.0	40.0	26.0	40.0
41	34.887916494160216	38.0	34.0	40.0	24.0	40.0
42	34.93276746507127	38.0	34.0	40.0	25.0	40.0
43	34.93871887877636	38.0	34.0	40.0	26.0	40.0
44	34.78451765783104	38.0	34.0	40.0	24.0	40.0
45	34.62239291529545	38.0	34.0	40.0	24.0	40.0
46	34.28432925488046	37.0	34.0	40.0	23.0	40.0
47	34.06491423662186	37.0	33.0	40.0	22.0	40.0
48	34.06914953495087	37.0	33.0	40.0	23.0	40.0
49	34.03275432396547	37.0	33.0	40.0	23.0	40.0
50	33.80345168335681	36.0	33.0	40.0	22.0	40.0
51	34.0441340976841	37.0	33.0	40.0	23.0	40.0
52	33.76405144530968	36.0	33.0	40.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	6.0
14	10.0
15	37.0
16	113.0
17	323.0
18	795.0
19	1667.0
20	3286.0
21	5697.0
22	9978.0
23	16088.0
24	23885.0
25	33318.0
26	46052.0
27	60664.0
28	73745.0
29	79819.0
30	82270.0
31	94317.0
32	118979.0
33	160046.0
34	287402.0
35	290896.0
36	416734.0
37	651315.0
38	1363760.0
39	425016.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.49152398675716	0.35537506552890125	0.0996887112249065	0.05341223648903566
8	98.83607012169418	0.8510161277635769	0.17691037059331388	0.13600337994893338
9	97.20652590140214	1.2983318331748392	0.7247154997694418	0.7704267656535769
10	51.67798732895956	26.334587625976813	13.072597779953831	8.9148272651098
11	31.22663509033215	30.334500018604793	23.806973641014192	14.631891250048866
12	29.267880264272815	25.401121656966268	23.42950832952995	21.90148974923096
13	24.039486432397016	25.05851089133907	25.721312471474615	25.180690204789297
14	22.39136097110417	27.879279867402	26.080102340482753	23.649256821011075
15	25.52115788685367	25.933642596776707	24.835088542321664	23.710110974047964
16	28.001812436384565	26.903752939674792	24.026321776225338	21.06811284771531
17	29.28964080506465	27.236920007404237	22.315811387922146	21.157627799608967
18	27.501791005548938	27.946421968914454	22.67728599897603	21.874501026560576
19	27.128470558977426	28.61042461785994	22.645752055122937	21.6153527680397
20	26.109045743765392	29.76076593335528	21.958481641780992	22.171706681098332
21	25.994661602395354	28.00831233817953	25.366973622173898	20.63005243725122
22	24.619767520179135	26.444355895057676	24.95199257315569	23.983884011607508
23	25.265400881443206	28.859516868893685	24.049071432507706	21.82601081715541
24	26.148516161911612	28.534733732464986	23.60578284016506	21.710967265458343
25	25.986748678471056	27.869883270241896	22.105035586962327	24.038332464324725
26	25.2145556351558	27.207128790843992	23.394724434779373	24.18359113922083
27	23.19678358482772	26.615590626764806	23.54718952253511	26.64043626587236
28	25.867418959648326	29.119489390323345	21.443458626005544	23.56963302402279
29	27.484787639259217	27.846144498468988	22.479981008982584	22.189086853289208
30	27.339128608093134	27.180799478500635	22.737056835047092	22.743015078359143
31	29.033247939695983	26.649691560819534	22.69087456178651	21.62618593769797
32	29.405626371514604	26.576096658249764	22.653994684210748	21.36428228602488
33	29.33059489644667	26.676562531645807	23.30174757866883	20.69109499323869
34	25.78958499069054	27.837360210898265	25.093341886827293	21.27971291158391
35	24.13780922223023	31.080316648839034	24.221365930811842	20.56050819811889
36	25.167101641978817	30.907927949059612	22.434976254163118	21.48999415479846
37	26.355900709761016	30.15436324748282	21.776884747792035	21.712851294964132
38	27.40554064817209	29.74293830415678	21.97861720712408	20.872903840547046
39	26.83849486766812	27.318357182791843	21.80314340902893	24.04000454051111
40	25.966000803538584	26.660053723101356	23.05696504512957	24.316980428230487
41	24.305417197138723	27.860204068655918	22.201804052453266	25.63257468175209
42	23.157148314099747	28.188613961883256	23.13371569712153	25.520522026895463
43	23.65806465895062	27.049788776742034	23.87611752387654	25.416029040430804
44	24.701911206631408	26.17357375433857	23.68952795169725	25.43498708733277
45	25.9064183704181	26.8534493518703	22.80090188492442	24.43923039278718
46	24.531430086726587	27.737341794509845	23.50870821987943	24.222519898884137
47	24.61578750784816	27.668268562753962	23.670664106270568	24.04527982312731
48	25.74980841774963	26.860844167680508	24.900935373548887	22.488412041020975
49	26.702161782555677	25.952388690359278	24.019327316685107	23.326122210399937
50	23.756740704316172	26.800625874601824	24.79590072860131	24.646732692480697
51	22.98296978628982	27.854057422393293	24.85543606098415	24.307536730332735
52	23.7956930143483	26.515454458532272	25.681371045951952	24.007481481167474
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	91.5
4	183.0
5	247.0
6	311.0
7	973.0
8	1635.0
9	15147.0
10	28659.0
11	24618.5
12	20578.0
13	19549.5
14	17033.0
15	15545.0
16	15952.5
17	16360.0
18	17574.0
19	18788.0
20	18988.0
21	19188.0
22	23593.5
23	27999.0
24	32580.5
25	37162.0
26	44217.0
27	51272.0
28	55912.5
29	60553.0
30	66914.0
31	73275.0
32	77829.5
33	82384.0
34	90343.5
35	98303.0
36	106550.5
37	114798.0
38	122616.5
39	137428.5
40	144422.0
41	150996.5
42	157571.0
43	162651.0
44	167731.0
45	177218.0
46	186705.0
47	194146.5
48	201588.0
49	220875.5
50	240163.0
51	253478.5
52	266794.0
53	269798.0
54	272802.0
55	294966.0
56	317130.0
57	317350.5
58	317571.0
59	306573.0
60	295575.0
61	286183.5
62	276792.0
63	232558.0
64	163211.5
65	138099.0
66	111193.5
67	84288.0
68	73303.0
69	62318.0
70	53620.0
71	44922.0
72	41626.5
73	38331.0
74	28426.0
75	18521.0
76	12256.0
77	5991.0
78	4516.0
79	3041.0
80	2001.0
81	961.0
82	730.5
83	500.0
84	296.0
85	92.0
86	56.0
87	20.0
88	13.5
89	6.0
90	5.0
91	5.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	4246218.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.168425166593797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.9866980228246	10.006251465701265
2	8.962638010870002	2.3604765588282146
3	3.651597971118594	1.4425738386348284
4	1.7774937544030964	0.9362717395578013
5	0.9585321239298943	0.6311179271873513
6	0.595695099178795	0.470661980138558
7	0.41924349887618834	0.3864543649072426
8	0.2963576396955719	0.3122050720703605
9	0.2476644096106248	0.2935215219947532
>10	3.2040514037826395	11.471245245006294
>50	2.3081106325413443	21.76427109970559
>100	1.5184388683176941	35.7530669948975
>500	0.050353331243099125	4.366686595870093
>1k	0.01958185103898299	4.611139904618755
>5k	0.0016784443747699708	1.4648288634650857
>10k+	0.0018649381941888564	3.7292268274163023
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	26477	0.6235431153087289	No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19959	0.4700418113248072	No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	16950	0.39917875153842786	No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	15754	0.3710125104269258	No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	14683	0.3457900654182145	No Hit
CAAAGTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	14111	0.33231925445184396	No Hit
CAAAGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	12082	0.28453555611134423	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAG	11021	0.2595486147908562	No Hit
CAAAGTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	10578	0.24911580140256576	No Hit
CAAAGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	10237	0.24108512563415255	No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8266	0.19466734868534777	No Hit
CAAAGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	7445	0.17533249588221803	No Hit
CAAAGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	7030	0.16555909282095269	No Hit
CAAAGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6861	0.16157908048997954	No Hit
CAAAGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	6747	0.1588943384442344	No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	6394	0.15058105824995324	No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT	5925	0.1395359352722823	No Hit
CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5768	0.13583852736717708	No Hit
CAAAGTGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC	5211	0.12272097193314145	No Hit
CAAAGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4978	0.11723373599753946	No Hit
CAAAGTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	4286	0.10093688077248977	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0412837965455377	0.0	0.0	0.0	0.0
8	0.17808788903443018	0.0	0.0	0.0	0.0
9	0.19681043224817943	0.0	0.0	0.0	0.0
10	0.3362757164139948	0.0	0.0	0.0	0.0
11	0.47545839615394214	0.0	0.0	0.0	0.0
12	0.5387146868107101	0.0	0.0	0.0	0.0
13	0.5825654735578814	0.0	0.0	0.0	0.0
14	0.6199399088789129	0.0	0.0	0.0	0.0
15	0.6607762484168265	0.0	0.0	0.0	0.0
16	0.7097139148296201	0.0	0.0	0.0	0.0
17	0.7552132273943543	0.0	0.0	0.0	0.0
18	0.7937416307876798	0.0	0.0	0.0	0.0
19	0.8515106855088458	0.0	0.0	0.0	0.0
20	0.8961386344271538	0.0	0.0	0.0	0.0
21	0.9413788929348422	0.0	0.0	0.0	0.0
22	0.9861716944349066	0.0	0.0	0.0	0.0
23	1.0400078375627442	0.0	0.0	0.0	0.0
24	1.0985540544550467	0.0	0.0	0.0	0.0
25	1.151071376928834	0.0	0.0	0.0	0.0
26	1.2105360582052076	0.0	0.0	0.0	0.0
27	1.2583197565457072	0.0	0.0	0.0	0.0
28	1.3211050398260287	0.0	0.0	0.0	0.0
29	1.356642546378919	0.0	0.0	0.0	0.0
30	1.3970549792780305	0.0	0.0	0.0	0.0
31	1.4252212203895325	0.0	0.0	0.0	0.0
32	1.4579091323149211	0.0	0.0	0.0	0.0
33	1.4857456682629107	0.0	0.0	0.0	0.0
34	1.5181509757624314	0.0	0.0	0.0	0.0
35	1.5681013080345851	0.0	0.0	0.0	0.0
36	1.5946896744349914	0.0	0.0	2.3550368822326128E-5	0.0
37	1.6220787533753567	0.0	0.0	2.3550368822326128E-5	0.0
38	1.647748655391692	0.0	0.0	2.3550368822326128E-5	0.0
39	1.6707338153622824	0.0	0.0	2.3550368822326128E-5	0.0
40	1.6939073782834513	0.0	0.0	2.3550368822326128E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGAT	20	6.313387E-4	46.0	13
AGTGGGG	227600	0.0	43.106808	4
AAGTGGG	435260	0.0	43.065155	3
CAAAGTG	451640	0.0	43.035114	1
AAAGTGG	447230	0.0	43.006397	2
GTGGGAT	30940	0.0	42.989334	5
GTGGGGT	53665	0.0	42.601326	5
GTGGGGG	78870	0.0	42.576393	5
AGTGGGC	38585	0.0	42.345985	4
TGGGGGG	36645	0.0	42.146267	6
AGTGGGA	115790	0.0	42.03524	4
GTGGGAC	15195	0.0	41.98881	5
GTGGGGC	37110	0.0	41.779305	5
GTGGGGA	62795	0.0	41.667007	5
GTGGGTG	22640	0.0	41.17447	5
TGGGATG	9150	0.0	40.997814	6
TGGGATT	9970	0.0	40.99398	6
TGGGTAT	4290	0.0	40.853146	6
TGGGTAC	4145	0.0	40.839565	6
GTGGGAG	30810	0.0	40.78189	5
>>END_MODULE