FastQCFastQC Report
Fri 17 Jun 2016
SRR1527864_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527864_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293925
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGA81802.783022879986391No Hit
TTCCGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC22450.7638002891894191No Hit
TTCCGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT17870.6079782257378583No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC14300.4865186697286723No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGG11690.3977205069320405No Hit
TTCCGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC8330.2834056306881007No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGC7390.2514246831674747No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG7130.24257888917240794Illumina Single End Adapter 2 (100% over 23bp)
TTCCGCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA6120.20821638173003318Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA5730.194947690737433No Hit
TTCCGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA5660.19256613081568427No Hit
TTCCGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG5570.1895041252020073No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG5310.18065833120694055No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG5280.1796376626690482No Hit
TTCCGCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA4980.16943097729012505Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTC4830.16432763460066344Illumina Single End Adapter 2 (100% over 25bp)
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG4720.16058518329505825No Hit
TTCCGCGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGT4420.15037849791613508No Hit
TTCCGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA4200.14289359530492474No Hit
TTCCGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4100.13949136684528368No Hit
TTCCGCGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC3980.1354086926937144No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGT3960.13472824700178618No Hit
TTCCGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT3950.13438802415582207No Hit
TTCCGCGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC3890.1323466870800374No Hit
TTCCGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG3830.13030535000425278No Hit
TTCCGCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGA3760.12792379008250404No Hit
TTCCGCGGGCTCCTGGCCACTCAGGGAGCTTGGCTTCTTAAGTCTGACTC3730.12690312154461172No Hit
TTCCGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA3670.12486178446882708No Hit
TTCCGCGGGAAGCACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA3660.12452156162286299Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTG3630.12350089308497064No Hit
TTCCGCGGGGGACGCTGTTGAGCTGGGAGGCGCGGTTGGAAGTCGTTACT3470.11805732754954494No Hit
TTCCGCGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAA3420.11635621331972441No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGC3410.11601599047376032Illumina Single End Adapter 2 (100% over 21bp)
TTCCGCGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCT3260.11091264778429871No Hit
TTCCGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA3250.1105724249383346No Hit
TTCCGCGGGATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACC3240.1102322020923705No Hit
TTCCGCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAAC3230.10989197924640641No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCC3200.10887131070851407Illumina Single End Adapter 2 (95% over 22bp)
TTCCGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3190.10853108786254997No Hit
TTCCGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG3170.10785064217062176No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC3150.10717019647869354No Hit
TTCCGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC3150.10717019647869354No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTG3100.10546908224887301No Hit
TTCCGCGGGGGAGTCAAGATGGAGGGTGTGGACTGCTCCATGGCTCTGGC3090.10512885940290889No Hit
TTCCGCGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA3080.1047886365569448Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA3070.10444841371098068Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT3060.10410819086501659No Hit
TTCCGCGGGGGACTCACCACTGGTTCCTAACCTTCTGAGAGGCTCTAAGC3040.10342774517308836No Hit
TTCCGCGGGAGGCACCAGGCTTAAGAGACTTCTACGCTAAGGGACTAGGG3040.10342774517308836No Hit
TTCCGCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCT3010.10240707663519606No Hit
TTCCGCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG3010.10240707663519606No Hit
TTCCGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC2960.10070596240537551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACTA502.7284841E-1144.015
GTTTGCG207.846909E-444.041
TTCTACG454.765752E-1044.030
TTCTAAC254.434732E-544.043
TGATAGC207.846909E-444.036
GATACAT502.7284841E-1144.022
TCGTTAC207.846909E-444.043
GCCTTAA254.434732E-544.010
ACAGTAT254.434732E-544.014
CAAACAT207.846909E-444.039
ACCGGAT254.434732E-544.023
CGAACCA502.7284841E-1144.015
GTATTGG351.4411671E-744.023
CTCGTCA207.846909E-444.012
GTATTCT207.846909E-444.037
AATCAGC254.434732E-544.030
CGAAATG207.846909E-444.026
AGATAGC254.434732E-544.021
CCCTATA408.276402E-944.022
AACCCTC254.434732E-544.025
Produced by FastQC (version 0.11.5)