##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527864_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	293925
Sequences flagged as poor quality	0
Sequence length	50
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53332312664796	34.0	31.0	34.0	31.0	34.0
2	32.95069830739134	34.0	31.0	34.0	31.0	34.0
3	32.96617164242579	34.0	31.0	34.0	31.0	34.0
4	36.41335714893255	37.0	37.0	37.0	35.0	37.0
5	36.23893170026367	37.0	37.0	37.0	35.0	37.0
6	36.19931615207961	37.0	37.0	37.0	35.0	37.0
7	36.479271923109636	37.0	37.0	37.0	35.0	37.0
8	36.60996512715829	37.0	37.0	37.0	35.0	37.0
9	38.64298375435911	39.0	39.0	39.0	38.0	39.0
10	37.98241728332057	39.0	38.0	39.0	35.0	39.0
11	37.84383771370248	39.0	38.0	39.0	35.0	39.0
12	37.262879986391084	39.0	37.0	39.0	35.0	39.0
13	36.94145105043804	39.0	37.0	39.0	33.0	39.0
14	37.83089903887046	40.0	37.0	40.0	33.0	40.0
15	37.83114399931956	40.0	37.0	40.0	33.0	40.0
16	37.48009866462533	39.0	36.0	40.0	33.0	40.0
17	37.611826146125715	39.0	37.0	40.0	33.0	40.0
18	37.488597431317515	39.0	36.0	40.0	33.0	40.0
19	36.707734966402995	39.0	35.0	40.0	31.0	40.0
20	36.91215105894361	39.0	35.0	40.0	32.0	40.0
21	36.94786425108446	39.0	35.0	40.0	32.0	40.0
22	36.948469847750275	39.0	35.0	40.0	32.0	40.0
23	36.80320489920898	38.0	35.0	40.0	32.0	40.0
24	36.725913073062856	38.0	35.0	40.0	32.0	40.0
25	36.54687760483117	38.0	35.0	40.0	32.0	40.0
26	36.22663604661053	38.0	35.0	40.0	31.0	40.0
27	36.019457344560685	38.0	35.0	40.0	31.0	40.0
28	35.69150293442205	37.0	34.0	40.0	30.0	40.0
29	35.77148252105129	37.0	35.0	40.0	30.0	40.0
30	35.733809645317685	37.0	35.0	40.0	30.0	40.0
31	35.7128178957217	37.0	35.0	40.0	31.0	40.0
32	35.1230007655014	36.0	34.0	40.0	29.0	40.0
33	35.182339032066004	36.0	35.0	40.0	29.0	40.0
34	34.75281449349324	35.0	34.0	40.0	28.0	40.0
35	34.74806838479204	35.0	34.0	39.0	28.0	40.0
36	34.510565620481415	35.0	34.0	39.0	28.0	40.0
37	34.14927277366675	35.0	34.0	39.0	26.0	40.0
38	34.073906608828786	35.0	34.0	39.0	26.0	40.0
39	33.8646321340478	35.0	34.0	39.0	26.0	40.0
40	33.54647614187293	35.0	33.0	38.0	23.0	40.0
41	33.52042868078591	35.0	33.0	37.0	24.0	40.0
42	33.41523177681381	35.0	33.0	37.0	23.0	40.0
43	33.086311133792634	35.0	33.0	37.0	22.0	40.0
44	32.75557029854555	35.0	33.0	37.0	22.0	40.0
45	32.62654418644212	35.0	33.0	36.0	22.0	39.0
46	32.412948881517394	35.0	33.0	36.0	20.0	39.0
47	32.185822914008675	35.0	33.0	36.0	20.0	39.0
48	32.00600493323127	35.0	32.0	35.0	20.0	39.0
49	31.885222420685547	35.0	32.0	35.0	20.0	39.0
50	31.327767287573362	35.0	31.0	35.0	18.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	2.0
15	4.0
16	14.0
17	22.0
18	56.0
19	158.0
20	248.0
21	416.0
22	758.0
23	1137.0
24	1690.0
25	2629.0
26	3412.0
27	4604.0
28	5898.0
29	6686.0
30	7717.0
31	8887.0
32	10750.0
33	14504.0
34	27053.0
35	41566.0
36	45618.0
37	48370.0
38	48417.0
39	13307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	98.45164582801735	1.3755209662328824	0.07110657480649825	0.10172663094326784
8	98.61155056562048	1.1690056987326698	0.14357404099685295	0.07586969464999575
9	96.90363187888067	2.3172578038615295	0.3000765501403419	0.479033767117462
10	50.80819937058774	40.821638173003315	2.4349749085651102	5.935187547843838
11	31.682912307561452	37.92191885685124	15.375350854809898	15.01981798077741
12	42.62720081653483	13.847069830739134	21.434379518584674	22.091349834141365
13	21.022029429276177	16.529726971166113	24.842731989453092	37.60551161010462
14	22.614952794080125	31.053500042527855	24.1905247937399	22.141022369652124
15	31.882963340988347	17.858977630347876	24.18882367951008	26.069235349153697
16	26.54418644211959	19.203198094752064	33.77732414731649	20.475291315811855
17	41.02883388619546	17.189419069490516	19.592072807689036	22.189674236624988
18	34.020583482180825	19.629497320745088	20.231691758101555	26.118227438972525
19	27.482180828442633	21.54427149783108	28.437866802755806	22.535680870970488
20	26.11244365059114	28.447733265288765	20.334439057582717	25.10538402653738
21	27.34847324997874	19.270562218252955	33.58067534234924	19.800289189419072
22	27.323636982223356	18.46491451900995	21.52045589861359	32.6909926001531
23	24.342944628731818	30.987156587564858	22.195117802160418	22.47478098154291
24	26.58263162371353	29.64123500893085	20.52530407416858	23.250829293187035
25	25.067619290635367	22.752062601003658	18.63026282214851	33.55005528621247
26	33.67729863060305	22.19001445947095	18.363868333758614	25.76881857616739
27	25.01148252105129	19.85948796461682	20.68418814323382	34.444841371098065
28	25.970230500978143	30.677894020583484	19.51212043888747	23.839755039550905
29	33.96886960959428	22.657480649825636	19.614527515522667	23.759122225057414
30	26.026027047716255	28.929148592327973	21.0985795696181	23.94624479033767
31	34.4009526239687	22.11686654758867	20.669218338011397	22.812962490431232
32	28.684868588925745	22.690822488730117	26.434634685719143	22.189674236624988
33	36.858722463213404	20.595389980437186	20.547078336310285	21.998809220039124
34	25.68580420175215	23.21068299736327	22.774857531683253	28.328655269201324
35	26.021263927872756	31.627115760823337	23.477417708599134	18.87420260270477
36	34.01173768818576	23.66283915964957	20.401462958237644	21.923960193927023
37	27.214085225822913	26.074338691843156	25.01148252105129	21.700093561282642
38	35.10181168665476	24.273539168155143	19.479799268520882	21.14484987666922
39	27.16985625584758	23.86595219869014	20.26945649400357	28.694735051458704
40	25.049587479799268	24.359275325338096	26.782682657140427	23.808454537722206
41	24.921323466870803	22.789146891213747	27.92617164242579	24.363357999489665
42	23.013013523858127	23.399846899719314	27.426384281704518	26.16075529471804
43	24.298715658756485	21.186016841030874	22.428510674491793	32.08675682572085
44	24.055796546738115	21.80011907799609	22.565960704261293	31.57812367100451
45	31.248447733265287	22.24615122905503	22.41115930934762	24.094241728332058
46	26.962660542655435	22.894615973462617	21.158458790507783	28.98426469337416
47	29.06319639363783	23.841456153780726	22.414901760653226	24.680445691928213
48	31.729522837458536	22.66292421536106	23.469932805987924	22.137620141192482
49	32.473590201582034	21.89980437186357	22.673130900739984	22.953474525814407
50	25.159479459045674	27.8179807774092	22.841541209492217	24.180998554052906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	39.0
8	78.0
9	53.5
10	29.0
11	41.0
12	53.0
13	157.0
14	261.0
15	483.0
16	705.0
17	822.5
18	940.0
19	844.0
20	748.0
21	664.0
22	580.0
23	663.0
24	746.0
25	791.5
26	837.0
27	1047.5
28	1258.0
29	1555.5
30	1853.0
31	2570.5
32	3288.0
33	3384.5
34	3481.0
35	3187.5
36	2894.0
37	3499.0
38	4104.0
39	4732.5
40	5361.0
41	4912.0
42	4463.0
43	5466.5
44	6470.0
45	6172.0
46	5874.0
47	7366.5
48	8859.0
49	9336.5
50	9814.0
51	9592.0
52	9370.0
53	12359.0
54	15348.0
55	16964.0
56	18580.0
57	21516.0
58	24452.0
59	30092.5
60	35733.0
61	31137.5
62	26542.0
63	25694.0
64	24846.0
65	24218.0
66	23590.0
67	20097.5
68	16605.0
69	13892.5
70	11180.0
71	9198.5
72	7217.0
73	6613.5
74	6010.0
75	5104.5
76	4199.0
77	4902.5
78	5606.0
79	3443.0
80	1280.0
81	871.0
82	462.0
83	284.0
84	106.0
85	96.5
86	87.0
87	48.0
88	9.0
89	6.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	293925.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.117802160415073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.79543152919994	11.156247342009015
2	9.427253291324407	2.8503870034872842
3	3.4297288173736917	1.555498851747895
4	1.658602453021267	1.0029769499021859
5	0.8371779003038146	0.632814493493238
6	0.6166310340947451	0.559326358764991
7	0.32181838640711147	0.3405630688100706
8	0.3623269944863283	0.43820702560176916
9	0.2700573871947789	0.367440673641235
>10	5.776977607741645	24.34566641149953
>50	2.5340384831776754	26.169260865867145
>100	0.9384494205018566	23.61963085821213
>500	0.020254304039608418	1.92293952538913
>1k	0.00900191290649263	2.25601769158799
>5k	0.0022504782266231575	2.783022879986391
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGA	8180	2.783022879986391	No Hit
TTCCGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2245	0.7638002891894191	No Hit
TTCCGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1787	0.6079782257378583	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1430	0.4865186697286723	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGG	1169	0.3977205069320405	No Hit
TTCCGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	833	0.2834056306881007	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGC	739	0.2514246831674747	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG	713	0.24257888917240794	Illumina Single End Adapter 2 (100% over 23bp)
TTCCGCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	612	0.20821638173003318	Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	573	0.194947690737433	No Hit
TTCCGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	566	0.19256613081568427	No Hit
TTCCGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	557	0.1895041252020073	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG	531	0.18065833120694055	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG	528	0.1796376626690482	No Hit
TTCCGCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA	498	0.16943097729012505	Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTC	483	0.16432763460066344	Illumina Single End Adapter 2 (100% over 25bp)
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	472	0.16058518329505825	No Hit
TTCCGCGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGT	442	0.15037849791613508	No Hit
TTCCGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	420	0.14289359530492474	No Hit
TTCCGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	410	0.13949136684528368	No Hit
TTCCGCGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC	398	0.1354086926937144	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGT	396	0.13472824700178618	No Hit
TTCCGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	395	0.13438802415582207	No Hit
TTCCGCGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC	389	0.1323466870800374	No Hit
TTCCGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	383	0.13030535000425278	No Hit
TTCCGCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGA	376	0.12792379008250404	No Hit
TTCCGCGGGCTCCTGGCCACTCAGGGAGCTTGGCTTCTTAAGTCTGACTC	373	0.12690312154461172	No Hit
TTCCGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	367	0.12486178446882708	No Hit
TTCCGCGGGAAGCACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA	366	0.12452156162286299	Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTG	363	0.12350089308497064	No Hit
TTCCGCGGGGGACGCTGTTGAGCTGGGAGGCGCGGTTGGAAGTCGTTACT	347	0.11805732754954494	No Hit
TTCCGCGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAA	342	0.11635621331972441	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGC	341	0.11601599047376032	Illumina Single End Adapter 2 (100% over 21bp)
TTCCGCGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCT	326	0.11091264778429871	No Hit
TTCCGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	325	0.1105724249383346	No Hit
TTCCGCGGGATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACC	324	0.1102322020923705	No Hit
TTCCGCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAAC	323	0.10989197924640641	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCC	320	0.10887131070851407	Illumina Single End Adapter 2 (95% over 22bp)
TTCCGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	319	0.10853108786254997	No Hit
TTCCGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	317	0.10785064217062176	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	315	0.10717019647869354	No Hit
TTCCGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	315	0.10717019647869354	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTG	310	0.10546908224887301	No Hit
TTCCGCGGGGGAGTCAAGATGGAGGGTGTGGACTGCTCCATGGCTCTGGC	309	0.10512885940290889	No Hit
TTCCGCGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	308	0.1047886365569448	Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA	307	0.10444841371098068	Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	306	0.10410819086501659	No Hit
TTCCGCGGGGGACTCACCACTGGTTCCTAACCTTCTGAGAGGCTCTAAGC	304	0.10342774517308836	No Hit
TTCCGCGGGAGGCACCAGGCTTAAGAGACTTCTACGCTAAGGGACTAGGG	304	0.10342774517308836	No Hit
TTCCGCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCT	301	0.10240707663519606	No Hit
TTCCGCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	301	0.10240707663519606	No Hit
TTCCGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	296	0.10070596240537551	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.007484902611210343	0.0	0.0	0.0	0.0
8	0.01939270221995407	0.0	0.0	0.0	0.0
9	0.030279833290805476	0.0	0.0	0.0	0.0
10	0.08709704856681126	0.0	0.0	0.0	0.0
11	0.24257888917240794	0.0	0.0	0.0	0.0
12	0.2609509228544697	0.0	0.0	0.0	0.0
13	0.33341838904482435	0.0	0.0	0.0	0.0
14	0.5862039635961555	0.0	0.0	0.0	0.0
15	0.6624138810921153	0.0	0.0	0.0	0.0
16	0.9635110997703495	0.0	0.0	0.0	0.0
17	1.0870119928553201	0.0	0.0	0.0	0.0
18	1.1394063111337926	0.0	0.0	0.0	0.0
19	1.217317342859573	0.0	0.0	0.0	0.0
20	1.284001020668538	0.0	0.0	0.0	0.0
21	1.3265288764140513	0.0	0.0	0.0	0.0
22	1.3530662583992514	0.0	0.0	0.0	0.0
23	1.4006974568342265	0.0	0.0	0.0	0.0
24	1.485412945479289	0.0	0.0	0.0	0.0
25	1.5201156757676277	0.0	0.0	0.0	0.0
26	1.7375180743386918	0.0	0.0	0.0	0.0
27	1.811346431912903	0.0	0.0	0.0	0.0
28	2.147146380879476	0.0	0.0	0.0	0.0
29	2.1906949051628817	0.0	0.0	0.0	0.0
30	2.34549630007655	0.0	0.0	0.0	0.0
31	2.374075019137535	0.0	0.0	0.0	0.0
32	2.4754614272348388	0.0	0.0	0.0	0.0
33	2.533299311048737	0.0	0.0	0.0	0.0
34	2.5829718465594964	0.0	0.0	0.0	0.0
35	2.856170791868674	0.0	0.0	0.0	0.0
36	2.896657310538403	0.0	0.0	0.0	0.0
37	2.981032576337501	0.0	0.0	0.0	0.0
38	3.046695585608574	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACTA	50	2.7284841E-11	44.0	15
GTTTGCG	20	7.846909E-4	44.0	41
TTCTACG	45	4.765752E-10	44.0	30
TTCTAAC	25	4.434732E-5	44.0	43
TGATAGC	20	7.846909E-4	44.0	36
GATACAT	50	2.7284841E-11	44.0	22
TCGTTAC	20	7.846909E-4	44.0	43
GCCTTAA	25	4.434732E-5	44.0	10
ACAGTAT	25	4.434732E-5	44.0	14
CAAACAT	20	7.846909E-4	44.0	39
ACCGGAT	25	4.434732E-5	44.0	23
CGAACCA	50	2.7284841E-11	44.0	15
GTATTGG	35	1.4411671E-7	44.0	23
CTCGTCA	20	7.846909E-4	44.0	12
GTATTCT	20	7.846909E-4	44.0	37
AATCAGC	25	4.434732E-5	44.0	30
CGAAATG	20	7.846909E-4	44.0	26
AGATAGC	25	4.434732E-5	44.0	21
CCCTATA	40	8.276402E-9	44.0	22
AACCCTC	25	4.434732E-5	44.0	25
>>END_MODULE