FastQCFastQC Report
Fri 17 Jun 2016
SRR1527863_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527863_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441647
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAG116442.636494757125034No Hit
TTCCGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT32110.7270512422817318No Hit
TTCCGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG27980.633537644317747No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC19710.4462840232131091No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGGAG15120.34235486712238505No Hit
TTCCGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC12570.2846164470719828No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC10270.2325386564382867Illumina Single End Adapter 2 (100% over 25bp)
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT9160.2074054618281116No Hit
TTCCGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG8090.18317796792460947No Hit
TTCCGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA7630.17276240979787025No Hit
TTCCGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG7520.17027173285451958No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT7450.16868675661784185No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA7060.15985617472778033No Hit
TTCCGCGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT6820.15442197048774248No Hit
TTCCGCGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTT6720.15215771872106004Illumina Single End Adapter 2 (100% over 27bp)
TTCCGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT6420.1453649634210127No Hit
TTCCGCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA6300.14264786130099377Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGG6300.14264786130099377No Hit
TTCCGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA6130.13879863329763362No Hit
TTCCGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA5940.13449655494093699No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT5670.1283830751708944No Hit
TTCCGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG5640.12770379964088968No Hit
TTCCGCGGGCTCCTGGCCACTCAGGGAGCTTGGCTTCTTAAGTCTGACTCTT5520.12498669752087074No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGCAG5500.12453384716753425No Hit
TTCCGCGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG5370.12159031987084709No Hit
TTCCGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC5250.11887321775082815No Hit
TTCCGCGGGGGACGCTGTTGAGCTGGGAGGCGCGGTTGGAAGTCGTTACTTT5230.11842036739749166No Hit
TTCCGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT5210.11796751704415517No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGTAG5180.11728824151415043No Hit
TTCCGCGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG5110.11570326527747274No Hit
TTCCGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC5020.11366543868745853No Hit
TTCCGCGGGATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTA5010.11343901351079028No Hit
TTCCGCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5000.11321258833412205Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA4960.11230688762744907No Hit
TTCCGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG4800.10868408480075717No Hit
TTCCGCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC4790.10845765962408892No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG4770.10800480927075243Illumina Single End Adapter 2 (100% over 23bp)
TTCCGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4750.10755195891741594No Hit
TTCCGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG4660.10551413232740174No Hit
TTCCGCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT4660.10551413232740174No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAA4620.10460843162072876No Hit
TTCCGCGGGGGAGTCAAGATGGAGGGTGTGGACTGCTCCATGGCTCTGGCCG4580.1037027309140558No Hit
TTCCGCGGGAAGCACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA4550.10302345538405107Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGCTCTTCAGATACTCTTGTGCTGTGCAGCAGTGGCTCTGTGTGT4520.10234417985404633No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGCGG4480.10143847914737335No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT4470.1012120539707051No Hit
TTCCGCGGGGGGTGGTGCTTTTGTATTGGTATTGTATGATGAGATCAAAAAA4440.10053277844070038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTT206.307211E-446.00000434
ATTTAGG206.307211E-446.00000443
AGCGTAA206.307211E-446.00000421
TACCCGT206.307211E-446.00000436
CGAACGT206.307211E-446.00000438
AATCATA206.307211E-446.00000444
AATTTAC206.307211E-446.00000444
GATGTAC405.5970304E-946.00000442
CATATAG206.307211E-446.00000429
CTGTAAT206.307211E-446.00000415
CGAGTAT206.307211E-446.00000420
ACGTTCG206.307211E-446.00000441
TTAGCGA206.307211E-446.00000427
CGTGCAA206.307211E-446.00000434
TTTAACG206.307211E-446.00000411
GGGTACG206.307211E-446.00000419
ATTACAT206.307211E-446.00000427
GTCTCGA206.307211E-446.00000437
CCAATCA206.307211E-446.00000415
GCACGCA206.307211E-446.00000423
Produced by FastQC (version 0.11.5)