##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527863_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	441647
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22340013630796	33.0	31.0	34.0	31.0	34.0
2	32.701084803021416	34.0	31.0	34.0	31.0	34.0
3	32.61658066283706	34.0	31.0	34.0	31.0	34.0
4	36.24370141764803	37.0	37.0	37.0	35.0	37.0
5	35.968409159351246	37.0	35.0	37.0	35.0	37.0
6	35.983738143811685	37.0	35.0	37.0	35.0	37.0
7	36.40459688393672	37.0	37.0	37.0	35.0	37.0
8	36.531906703770204	37.0	37.0	37.0	35.0	37.0
9	38.574891259308906	39.0	39.0	39.0	37.0	39.0
10	37.69219308633365	39.0	37.0	39.0	35.0	39.0
11	37.492703448681866	39.0	37.0	39.0	35.0	39.0
12	36.92994631459061	39.0	37.0	39.0	33.0	39.0
13	36.635516600361825	39.0	35.0	39.0	32.0	39.0
14	37.53764205349521	40.0	37.0	40.0	33.0	40.0
15	37.54136448339964	40.0	36.0	40.0	33.0	40.0
16	37.10047390789477	39.0	35.0	40.0	32.0	40.0
17	37.291590342513366	39.0	36.0	40.0	33.0	40.0
18	37.23999710175774	39.0	35.0	40.0	33.0	40.0
19	36.3778356923063	39.0	35.0	40.0	30.0	40.0
20	36.722081209653865	39.0	35.0	40.0	31.0	40.0
21	36.77672213328744	39.0	35.0	40.0	31.0	40.0
22	36.767166990832045	38.0	35.0	40.0	32.0	40.0
23	36.682637943878255	38.0	35.0	40.0	32.0	40.0
24	36.59860476806137	38.0	35.0	40.0	32.0	40.0
25	36.4503211841131	38.0	35.0	40.0	31.0	40.0
26	36.004843234528934	38.0	35.0	40.0	31.0	40.0
27	35.840913670872894	37.0	35.0	40.0	30.0	40.0
28	35.57670492497402	37.0	34.0	40.0	30.0	40.0
29	35.41268026274378	37.0	34.0	40.0	30.0	40.0
30	34.99165849649154	36.0	34.0	40.0	28.0	40.0
31	34.94328275749637	36.0	34.0	40.0	28.0	40.0
32	33.9086283842073	35.0	33.0	39.0	24.0	40.0
33	34.15176373891366	35.0	33.0	39.0	26.0	40.0
34	33.87188637078934	35.0	33.0	39.0	24.0	40.0
35	33.768969335238324	35.0	33.0	39.0	25.0	40.0
36	33.58559664166178	35.0	33.0	38.0	24.0	40.0
37	33.100374280817036	35.0	33.0	38.0	23.0	40.0
38	33.07163866164606	35.0	32.0	38.0	23.0	40.0
39	32.86194177703007	35.0	32.0	37.0	22.0	40.0
40	32.55402391502716	35.0	32.0	37.0	21.0	40.0
41	32.621247285728195	35.0	32.0	37.0	22.0	40.0
42	32.572312276546654	35.0	32.0	37.0	22.0	40.0
43	32.02874920468157	35.0	31.0	36.0	20.0	39.0
44	31.95044911433792	35.0	31.0	36.0	21.0	39.0
45	31.654411781354792	35.0	31.0	36.0	20.0	39.0
46	31.442095157444747	35.0	31.0	35.0	19.0	39.0
47	31.178995894911548	34.0	30.0	35.0	19.0	38.0
48	31.026570994482018	34.0	30.0	35.0	18.0	38.0
49	31.289178914381846	35.0	30.0	35.0	18.0	38.0
50	31.03569592910175	35.0	30.0	35.0	18.0	37.0
51	31.148489630859036	35.0	31.0	35.0	18.0	37.0
52	30.57933145702337	34.0	30.0	35.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	3.0
16	21.0
17	54.0
18	131.0
19	290.0
20	584.0
21	1138.0
22	1841.0
23	2736.0
24	4034.0
25	5784.0
26	7567.0
27	9529.0
28	11078.0
29	12804.0
30	14287.0
31	16147.0
32	19578.0
33	26332.0
34	46346.0
35	62913.0
36	66191.0
37	64674.0
38	56623.0
39	10959.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	98.53774620907649	1.34496554940937	0.05207779063369615	0.0652104508804543
8	98.69601740756758	1.114011869207761	0.1190996429274964	0.0708710802971604
9	97.01775399810255	2.1924749856786074	0.27601229035858954	0.5137587258602458
10	50.73327793464011	40.85027182342459	2.431127121886937	5.985323120048364
11	31.784434174804765	37.882064182480576	15.352079828460285	14.98142181425437
12	42.71624170434759	13.712761549382199	21.381555857958958	22.189440888311253
13	20.92961120532914	16.195287186372827	24.886844017960044	37.98825759033799
14	22.666065885197906	31.044476697452943	24.077374011371074	22.212083405978078
15	32.53888286346335	17.522591572002074	24.296327157209262	25.642198407325306
16	26.679678566819202	18.840386100211255	34.128614028851096	20.351321304118446
17	41.40388138037845	17.005210043315135	19.35663550301485	22.234273073291565
18	34.79588902449241	19.49452843560581	20.18852160209398	25.5210609378078
19	27.442052136661175	20.88228834340548	29.11556061741617	22.56009890251717
20	25.79594110228304	29.14024096167301	20.333433715161657	24.730384220882286
21	27.36755825353733	18.798950292880967	33.99253249767348	19.840958955908224
22	27.174417577839314	18.044048753869042	21.568809479063596	33.21272418922805
23	24.30696914051267	31.284260959544614	22.011244274273345	22.39752562566937
24	26.30969982814329	29.940427536018582	20.474723025402643	23.275149610435484
25	24.907675134213523	22.22725389281485	18.61667802566303	34.248392947308595
26	34.459647637140066	21.669342257504294	18.25802054582053	25.612989559535105
27	24.931676202940356	19.192477249930374	20.642956931667143	35.23288961546212
28	25.95353302524414	30.801069632534585	19.511057473502593	23.73433986871868
29	34.68335571168829	22.039320996180205	19.559965311662936	23.717357980468563
30	26.027800483191328	29.325230331010964	21.015426347286407	23.6315428385113
31	35.39184008948323	21.57718721060032	20.35358555588513	22.677387144031318
32	29.021141318745514	22.495341301990052	26.484726489707842	21.99879088955659
33	37.8512703584537	19.98768247038925	20.43328721807235	21.727759953084703
34	25.774430710499562	22.525229425310258	22.486057869746652	29.214281994443525
35	25.809979463236477	31.815680849184986	23.439081438343294	18.935258249235247
36	34.75558534304547	23.190013744008223	20.283393751117973	21.771007161828337
37	27.316159738433637	25.71420161350581	25.452001258923985	21.517637389136574
38	35.976696320817304	23.897592421096487	19.296632831197766	20.829078426888444
39	27.21494768446293	23.452893374120055	20.07236548646317	29.259793454953844
40	24.876654885009973	23.962576446800274	27.335632303627104	23.82513636456265
41	24.86057869746653	22.211856980801407	28.790187638543905	24.137376683188158
42	22.986457510183474	22.854678057362555	28.148045837512765	26.01081859494121
43	24.316931848286075	20.810737987578314	22.465000328316506	32.407329835819105
44	23.884912611203067	21.242983649837992	22.497152703403398	32.37495103555554
45	31.514308939039548	21.6650401791476	22.505983285293457	24.314667596519392
46	26.759833079359762	22.33820222938229	21.24705930301802	29.65490538823993
47	30.064961383186116	23.137935953374527	22.221140413044807	24.575962250394547
48	32.762590938011584	21.95758150740297	23.301414930929	21.97841262365645
49	33.16970340566108	21.305476998598426	22.548777643683756	22.976041952056732
50	25.401508444526964	27.139774525809074	23.098085122280917	24.360631907383045
51	23.327453826245847	28.393943579374476	23.620900855207893	24.65770173917178
52	29.933181930365198	23.13612455196118	23.63425994063132	23.2964335770423
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	59.0
8	118.0
9	76.5
10	35.0
11	52.5
12	70.0
13	185.5
14	583.0
15	865.0
16	999.5
17	1134.0
18	1052.0
19	970.0
20	880.0
21	790.0
22	867.0
23	944.0
24	979.0
25	1014.0
26	1208.5
27	1403.0
28	1720.5
29	2038.0
30	2925.0
31	3812.0
32	4236.5
33	4661.0
34	4425.5
35	4190.0
36	4445.5
37	4701.0
38	5362.5
39	6472.0
40	6920.0
41	7400.0
42	7880.0
43	8202.0
44	8524.0
45	9409.0
46	10294.0
47	11764.5
48	13235.0
49	12720.5
50	12206.0
51	14126.0
52	16046.0
53	18831.0
54	21616.0
55	24642.0
56	27668.0
57	30900.5
58	34133.0
59	41690.5
60	49248.0
61	46401.5
62	43555.0
63	38924.0
64	34910.5
65	35528.0
66	30958.0
67	26388.0
68	21802.5
69	17217.0
70	15476.5
71	13736.0
72	11609.5
73	9483.0
74	8857.0
75	8231.0
76	8300.0
77	8369.0
78	5487.0
79	2605.0
80	1700.0
81	795.0
82	612.0
83	429.0
84	294.5
85	160.0
86	83.0
87	6.0
88	6.0
89	3.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	441647.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.913147830733594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.27539180106433	10.33404506313866
2	9.578986769085555	2.6654771797385695
3	4.037625921525867	1.6852825899417407
4	1.933373476329194	1.0759724395274959
5	1.043175419467183	0.7256926912217223
6	0.6770062004654418	0.5651572409639373
7	0.42150145653978227	0.4105088452995265
8	0.3319934252282455	0.3695258883225744
9	0.21807411264992593	0.27306876306190236
>10	3.4566374273764384	12.820872778485986
>50	2.446010382931632	24.557621811084417
>100	1.5281462073006005	35.6986462038687
>500	0.04068546877797126	3.5152508677744896
>1k	0.009764512506713103	2.6663828804452425
>5k	0.0	0.0
>10k+	0.0016274187511188505	2.636494757125034
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAG	11644	2.636494757125034	No Hit
TTCCGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3211	0.7270512422817318	No Hit
TTCCGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2798	0.633537644317747	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1971	0.4462840232131091	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGGAG	1512	0.34235486712238505	No Hit
TTCCGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1257	0.2846164470719828	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC	1027	0.2325386564382867	Illumina Single End Adapter 2 (100% over 25bp)
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	916	0.2074054618281116	No Hit
TTCCGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	809	0.18317796792460947	No Hit
TTCCGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	763	0.17276240979787025	No Hit
TTCCGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	752	0.17027173285451958	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	745	0.16868675661784185	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA	706	0.15985617472778033	No Hit
TTCCGCGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT	682	0.15442197048774248	No Hit
TTCCGCGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTT	672	0.15215771872106004	Illumina Single End Adapter 2 (100% over 27bp)
TTCCGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	642	0.1453649634210127	No Hit
TTCCGCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA	630	0.14264786130099377	Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGG	630	0.14264786130099377	No Hit
TTCCGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	613	0.13879863329763362	No Hit
TTCCGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	594	0.13449655494093699	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT	567	0.1283830751708944	No Hit
TTCCGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	564	0.12770379964088968	No Hit
TTCCGCGGGCTCCTGGCCACTCAGGGAGCTTGGCTTCTTAAGTCTGACTCTT	552	0.12498669752087074	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGCAG	550	0.12453384716753425	No Hit
TTCCGCGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG	537	0.12159031987084709	No Hit
TTCCGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	525	0.11887321775082815	No Hit
TTCCGCGGGGGACGCTGTTGAGCTGGGAGGCGCGGTTGGAAGTCGTTACTTT	523	0.11842036739749166	No Hit
TTCCGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	521	0.11796751704415517	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGTAG	518	0.11728824151415043	No Hit
TTCCGCGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG	511	0.11570326527747274	No Hit
TTCCGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	502	0.11366543868745853	No Hit
TTCCGCGGGATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTA	501	0.11343901351079028	No Hit
TTCCGCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	500	0.11321258833412205	Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA	496	0.11230688762744907	No Hit
TTCCGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	480	0.10868408480075717	No Hit
TTCCGCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC	479	0.10845765962408892	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG	477	0.10800480927075243	Illumina Single End Adapter 2 (100% over 23bp)
TTCCGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	475	0.10755195891741594	No Hit
TTCCGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	466	0.10551413232740174	No Hit
TTCCGCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	466	0.10551413232740174	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAA	462	0.10460843162072876	No Hit
TTCCGCGGGGGAGTCAAGATGGAGGGTGTGGACTGCTCCATGGCTCTGGCCG	458	0.1037027309140558	No Hit
TTCCGCGGGAAGCACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA	455	0.10302345538405107	Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGCTCTTCAGATACTCTTGTGCTGTGCAGCAGTGGCTCTGTGTGT	452	0.10234417985404633	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGCGG	448	0.10143847914737335	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT	447	0.1012120539707051	No Hit
TTCCGCGGGGGGTGGTGCTTTTGTATTGGTATTGTATGATGAGATCAAAAAA	444	0.10053277844070038	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.007698456006720299	0.0	0.0	0.0	0.0
8	0.018566864486796016	0.0	0.0	0.0	0.0
9	0.02762387155352578	0.0	0.0	0.0	0.0
10	0.07811668595054422	0.0	0.0	0.0	0.0
11	0.21148111500813999	0.0	0.0	0.0	0.0
12	0.2264251766682441	0.0	0.0	0.0	0.0
13	0.2868806988386653	0.0	0.0	0.0	0.0
14	0.4970032627867958	0.0	0.0	0.0	0.0
15	0.5615344381372454	0.0	0.0	0.0	0.0
16	0.8212441157757213	0.0	0.0	0.0	0.0
17	0.9269846732797913	0.0	0.0	0.0	0.0
18	0.9770246373234732	0.0	0.0	0.0	0.0
19	1.042461513380596	0.0	0.0	0.0	0.0
20	1.1031434607276853	0.0	0.0	0.0	0.0
21	1.140050764524609	0.0	0.0	0.0	0.0
22	1.1692596123148125	0.0	0.0	0.0	0.0
23	1.212280395881779	0.0	0.0	0.0	0.0
24	1.2799815237055838	0.0	0.0	0.0	0.0
25	1.3121338987924744	0.0	0.0	0.0	0.0
26	1.5079916766105057	0.0	0.0	0.0	0.0
27	1.5727492771376235	0.0	0.0	0.0	0.0
28	1.8951787287132031	0.0	0.0	0.0	0.0
29	1.9289160800367715	0.0	0.0	0.0	0.0
30	2.070431815454424	0.0	0.0	0.0	0.0
31	2.0926214827679117	0.0	0.0	0.0	0.0
32	2.1831915534352095	0.0	0.0	0.0	0.0
33	2.2264387621788444	0.0	0.0	0.0	0.0
34	2.2683274198624694	0.0	0.0	0.0	0.0
35	2.5178479645508745	0.0	0.0	0.0	0.0
36	2.551132465521106	0.0	0.0	0.0	0.0
37	2.621324270288262	0.0	0.0	0.0	0.0
38	2.6767984385719816	0.0	0.0	0.0	0.0
39	2.7227627494356352	0.0	0.0	0.0	0.0
40	2.748575219575815	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTT	20	6.307211E-4	46.000004	34
ATTTAGG	20	6.307211E-4	46.000004	43
AGCGTAA	20	6.307211E-4	46.000004	21
TACCCGT	20	6.307211E-4	46.000004	36
CGAACGT	20	6.307211E-4	46.000004	38
AATCATA	20	6.307211E-4	46.000004	44
AATTTAC	20	6.307211E-4	46.000004	44
GATGTAC	40	5.5970304E-9	46.000004	42
CATATAG	20	6.307211E-4	46.000004	29
CTGTAAT	20	6.307211E-4	46.000004	15
CGAGTAT	20	6.307211E-4	46.000004	20
ACGTTCG	20	6.307211E-4	46.000004	41
TTAGCGA	20	6.307211E-4	46.000004	27
CGTGCAA	20	6.307211E-4	46.000004	34
TTTAACG	20	6.307211E-4	46.000004	11
GGGTACG	20	6.307211E-4	46.000004	19
ATTACAT	20	6.307211E-4	46.000004	27
GTCTCGA	20	6.307211E-4	46.000004	37
CCAATCA	20	6.307211E-4	46.000004	15
GCACGCA	20	6.307211E-4	46.000004	23
>>END_MODULE