FastQCFastQC Report
Fri 17 Jun 2016
SRR1527862_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527862_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442884
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAG107882.435852277345761No Hit
TTCCGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT31020.7004091364781748No Hit
TTCCGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG26890.607156727269443No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGGAG20140.454746615366552No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC19430.4387153295219516No Hit
TTCCGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC10870.24543672835324826No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC9990.22556696561627873Illumina Single End Adapter 2 (100% over 25bp)
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT8000.18063420669972272No Hit
TTCCGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG7840.17702152256572826No Hit
TTCCGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA7640.17250566739823522No Hit
TTCCGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG7540.17024773981448865No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT7310.16505450637187166No Hit
TTCCGCGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT6800.15353907569476433No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA6760.1526359046612657No Hit
TTCCGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT6230.14066888846740908No Hit
TTCCGCGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTT6220.1404430957090344Illumina Single End Adapter 2 (100% over 27bp)
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGCAG6050.1366046188166653No Hit
TTCCGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA6020.13592724054154137No Hit
TTCCGCGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGG5780.13050821434054966No Hit
TTCCGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA5710.1289276650319271No Hit
TTCCGCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA5610.12666973744818055Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT5290.11944436918019165No Hit
TTCCGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5290.11944436918019165No Hit
TTCCGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT5250.11854119814669305No Hit
TTCCGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC5030.11357375746245066No Hit
TTCCGCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4960.11199320815382809Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG4900.11063845160358017No Hit
TTCCGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG4890.11041265884520551No Hit
TTCCGCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA4810.10860631677820828No Hit
TTCCGCGGGCTCCTGGCCACTCAGGGAGCTTGGCTTCTTAAGTCTGACTCTT4750.10725156022796035No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGTAG4690.10589680367771245No Hit
TTCCGCGGGGGACGCTGTTGAGCTGGGAGGCGCGGTTGGAAGTCGTTACTTT4660.1052194254025885No Hit
TTCCGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG4660.1052194254025885No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAA4630.10454204712746452No Hit
TTCCGCGGGATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTA4620.10431625436908988No Hit
TTCCGCGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG4540.10250991230209267No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT4450.10047777747672078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATTA206.307231E-446.00000438
TATTTCG351.01767E-746.00000419
AACCGTA206.307231E-446.00000441
ACACGTT206.307231E-446.00000440
CGAACCA206.307231E-446.00000415
GTACATA206.307231E-446.00000412
TTAATGC206.307231E-446.00000426
GTATTAG206.307231E-446.00000428
CCGATCA206.307231E-446.00000429
TTAGCGA206.307231E-446.00000427
TCATTCG206.307231E-446.00000427
TCCGAAT206.307231E-446.00000415
TTTCGAC206.307231E-446.00000420
ATAATAG206.307231E-446.00000433
CGGTTCA206.307231E-446.00000442
TCTTATA206.307231E-446.00000418
TTCACTA206.307231E-446.00000426
CCGGATA551.8189894E-1246.00000420
ATAGCGC206.307231E-446.00000437
ATTAAGC206.307231E-446.00000422
Produced by FastQC (version 0.11.5)