##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527862_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	442884
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.193109256599925	33.0	31.0	34.0	31.0	34.0
2	32.685037165488026	34.0	31.0	34.0	31.0	34.0
3	32.59757408260402	34.0	31.0	34.0	31.0	34.0
4	36.2212294867279	37.0	35.0	37.0	35.0	37.0
5	35.9748421708619	37.0	35.0	37.0	35.0	37.0
6	35.92565999223273	37.0	35.0	37.0	35.0	37.0
7	36.36583845882895	37.0	35.0	37.0	35.0	37.0
8	36.50321303095167	37.0	37.0	37.0	35.0	37.0
9	38.529192745730256	39.0	39.0	39.0	37.0	39.0
10	37.602914081339584	39.0	37.0	39.0	35.0	39.0
11	37.4496978892893	39.0	37.0	39.0	35.0	39.0
12	36.86882343909466	39.0	35.0	39.0	33.0	39.0
13	36.427940499092315	39.0	35.0	39.0	32.0	39.0
14	37.4569345471952	40.0	36.0	40.0	33.0	40.0
15	37.4762443438914	40.0	36.0	40.0	33.0	40.0
16	37.07636085295472	39.0	35.0	40.0	32.0	40.0
17	37.29656298263202	39.0	36.0	40.0	33.0	40.0
18	37.267223471608816	39.0	35.0	40.0	33.0	40.0
19	36.337596300611445	39.0	35.0	40.0	30.0	40.0
20	36.71452569973176	39.0	35.0	40.0	31.0	40.0
21	36.81856422900805	39.0	35.0	40.0	31.0	40.0
22	36.69212931602858	38.0	35.0	40.0	31.0	40.0
23	36.64383901879499	38.0	35.0	40.0	31.0	40.0
24	36.60082098246945	38.0	35.0	40.0	32.0	40.0
25	36.43656352453464	38.0	35.0	40.0	31.0	40.0
26	35.9581854390766	38.0	35.0	40.0	31.0	40.0
27	35.76741539545344	37.0	35.0	40.0	30.0	40.0
28	35.525991004416504	37.0	34.0	40.0	30.0	40.0
29	35.34960170157423	37.0	34.0	40.0	29.0	40.0
30	34.92122542245825	36.0	34.0	40.0	27.0	40.0
31	34.903157485933114	36.0	34.0	40.0	28.0	40.0
32	33.88188329223905	35.0	33.0	39.0	24.0	40.0
33	33.953567977167836	35.0	33.0	39.0	25.0	40.0
34	33.76550970457276	35.0	33.0	39.0	24.0	40.0
35	33.748338165298364	35.0	33.0	39.0	25.0	40.0
36	33.42820016076445	35.0	33.0	38.0	23.0	40.0
37	33.023721787194845	35.0	32.0	38.0	22.0	40.0
38	32.74620442373172	35.0	32.0	37.0	22.0	40.0
39	32.66129731487252	35.0	32.0	37.0	22.0	40.0
40	32.42392364592083	35.0	31.0	37.0	21.0	40.0
41	32.319015814524796	35.0	31.0	37.0	21.0	40.0
42	32.386428500465136	35.0	31.0	36.0	22.0	40.0
43	31.985526684188184	35.0	31.0	36.0	20.0	39.0
44	31.896632075216083	35.0	31.0	36.0	20.0	39.0
45	31.602342825660894	35.0	31.0	36.0	20.0	39.0
46	31.24218982848782	34.0	30.0	35.0	18.0	39.0
47	30.893816439519153	34.0	30.0	35.0	18.0	38.0
48	30.887320381860714	34.0	30.0	35.0	18.0	38.0
49	30.86267058642895	34.0	29.0	35.0	18.0	37.0
50	30.257783076381173	34.0	29.0	35.0	15.0	37.0
51	30.68359886561718	34.0	30.0	35.0	17.0	37.0
52	30.346476729798322	34.0	29.0	35.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	1.0
14	2.0
15	10.0
16	16.0
17	50.0
18	139.0
19	305.0
20	588.0
21	1147.0
22	1923.0
23	2882.0
24	4368.0
25	6052.0
26	7746.0
27	9799.0
28	11506.0
29	13035.0
30	14483.0
31	17022.0
32	20468.0
33	28115.0
34	48045.0
35	63254.0
36	66071.0
37	63510.0
38	52989.0
39	9356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	98.57005446121332	1.31592019580748	0.04764227201705187	0.0663830709621481
8	98.72968994138421	1.07816042123897	0.12260546779743679	0.06954416957939326
9	97.07891908490711	2.1339673593988495	0.29691747726266926	0.49019607843137253
10	50.671959248922974	40.89964866646797	2.450980392156863	5.9774116924522
11	31.73584956783266	37.86296185908726	15.450321077302409	14.950867495777675
12	42.60483557771335	13.693427624389232	21.385961109455298	22.315775688442123
13	21.145717614544665	16.203114133723503	24.88981313391317	37.76135511781866
14	22.56663144299636	31.07247044372793	24.097054759259763	22.26384335401595
15	32.626827792379046	17.574353555332774	24.32849233659378	25.470326315694404
16	26.646029208551226	18.81576214087662	34.148219398307454	20.3899892522647
17	41.471355930672594	16.908264918127546	19.445723936741903	22.17465521445796
18	34.82333974584767	19.382276171638622	20.125811724966358	25.668572357547347
19	27.440819718030003	20.912247902385275	29.283740211883924	22.3631921677008
20	25.830691558060348	28.99766078702324	20.39202138709007	24.779626267826337
21	27.185673901066643	18.799053476756892	34.15905745070944	19.85621517146702
22	27.3785009167186	18.08261305443412	21.525501034130833	33.013384994716446
23	24.362135457591606	31.26755538696363	21.977763929155262	22.392545226289503
24	26.161477949079213	30.00447069661582	20.502434045935278	23.331617308369687
25	24.801753958147053	22.318936787059364	18.586130905609597	34.29317834918398
26	34.57812881025279	21.61062490403808	18.204315351198055	25.60693093451107
27	24.792948040570444	19.425176795729808	20.596363833419133	35.18551133028062
28	25.97587630169525	30.824775787790937	19.519106583213663	23.68024132730015
29	34.60341759919076	22.115723304522177	19.49426937979245	23.786589716494614
30	26.05625852367663	29.372476765925164	20.8639282520931	23.707336458305107
31	35.28215063086497	21.533855366190696	20.333766855429413	22.850227147514925
32	29.057270075234147	22.580179008498842	26.462911281509378	21.899639634757634
33	37.72455089820359	20.125134346691233	20.383892847788584	21.766421907316587
34	25.598576602451207	22.630982379133137	22.422801455911706	29.347639562503954
35	25.713279323705528	31.96141653344894	23.27313698395065	19.052167158894882
36	34.98568473911905	23.171530242682056	20.19061424661988	21.652170771579012
37	27.409886110132675	25.7990805718879	25.39807263301451	21.392960684964912
38	35.99023672112788	23.791557157178854	19.221285934917496	20.996920186775768
39	27.147289132142955	23.369776284535	20.073653597781814	29.40928098554023
40	24.796560724704435	23.904227743607805	27.537459018614356	23.7617525130734
41	25.141797852259284	22.04798547700978	28.8759584902593	23.934258180471634
42	22.836453789254072	22.836453789254072	28.13039080210619	26.196701619385664
43	24.112860252345985	20.935052970981115	22.541116861300026	32.410969915372874
44	23.982126245247063	21.26132350683249	22.392771019047878	32.363779228872566
45	31.62137263933671	21.6103991112797	22.448541830366416	24.31968641901717
46	27.01158768435979	22.43318792279694	21.038691847075082	29.516532545768193
47	30.357610570713778	23.04757001833437	22.14439898483576	24.450420426116093
48	32.80813937735389	22.052952917694025	23.228655810550844	21.91025189440124
49	33.5981882389068	21.301063032306427	22.326613740844103	22.774134987942666
50	25.716892007839526	27.216607508963975	22.889515087472116	24.176985395724387
51	23.4856079695812	28.520109103060847	23.37000207729338	24.624280850064576
52	29.928830122560306	23.1746913412993	23.55831323777784	23.33816529836255
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	53.5
8	107.0
9	74.0
10	41.0
11	45.5
12	50.0
13	190.5
14	628.0
15	925.0
16	1030.5
17	1136.0
18	1081.5
19	1027.0
20	916.5
21	806.0
22	858.0
23	910.0
24	941.5
25	973.0
26	1178.5
27	1384.0
28	1661.5
29	1939.0
30	2831.0
31	3723.0
32	4182.0
33	4641.0
34	4547.5
35	4454.0
36	4558.0
37	4662.0
38	5376.0
39	6529.0
40	6968.0
41	7436.5
42	7905.0
43	8132.5
44	8360.0
45	9273.0
46	10186.0
47	11778.0
48	13370.0
49	12988.0
50	12606.0
51	14296.0
52	15986.0
53	18763.0
54	21540.0
55	24621.5
56	27703.0
57	31019.5
58	34336.0
59	41292.0
60	48248.0
61	46120.5
62	43993.0
63	39414.5
64	35201.0
65	35566.0
66	31200.0
67	26834.0
68	22171.0
69	17508.0
70	15566.5
71	13625.0
72	11548.5
73	9472.0
74	8877.5
75	8283.0
76	8276.5
77	8270.0
78	5444.0
79	2618.0
80	1700.5
81	783.0
82	638.5
83	494.0
84	328.5
85	163.0
86	93.0
87	23.0
88	14.0
89	3.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	442884.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.624479547691944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.29963103412935	13.623657662051464
2	9.545710771753612	3.3647636852990854
3	3.4718663523624063	1.8356951255859324
4	1.6065388951521982	1.1325764760072614
5	0.8788562058009634	0.7744691612250612
6	0.5291073075740494	0.5595144552523912
7	0.33309418878753716	0.41094282024186923
8	0.28569232346007994	0.4028142809403817
9	0.1998565132725223	0.3170130327580134
>10	2.8364251306754125	13.474182856007442
>50	1.8947934816029517	24.193242474327363
>100	1.0863995080455058	32.220852412821415
>500	0.024341498411396944	2.8079587431471897
>1k	0.0064056574766834075	2.4464645369893696
>5k	0.0	0.0
>10k+	0.0012811314953366814	2.435852277345761
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAG	10788	2.435852277345761	No Hit
TTCCGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3102	0.7004091364781748	No Hit
TTCCGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2689	0.607156727269443	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGGAG	2014	0.454746615366552	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1943	0.4387153295219516	No Hit
TTCCGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1087	0.24543672835324826	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC	999	0.22556696561627873	Illumina Single End Adapter 2 (100% over 25bp)
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	800	0.18063420669972272	No Hit
TTCCGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	784	0.17702152256572826	No Hit
TTCCGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	764	0.17250566739823522	No Hit
TTCCGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	754	0.17024773981448865	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	731	0.16505450637187166	No Hit
TTCCGCGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT	680	0.15353907569476433	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA	676	0.1526359046612657	No Hit
TTCCGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	623	0.14066888846740908	No Hit
TTCCGCGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTT	622	0.1404430957090344	Illumina Single End Adapter 2 (100% over 27bp)
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGCAG	605	0.1366046188166653	No Hit
TTCCGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	602	0.13592724054154137	No Hit
TTCCGCGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGG	578	0.13050821434054966	No Hit
TTCCGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	571	0.1289276650319271	No Hit
TTCCGCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA	561	0.12666973744818055	Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT	529	0.11944436918019165	No Hit
TTCCGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	529	0.11944436918019165	No Hit
TTCCGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	525	0.11854119814669305	No Hit
TTCCGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	503	0.11357375746245066	No Hit
TTCCGCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	496	0.11199320815382809	Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG	490	0.11063845160358017	No Hit
TTCCGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	489	0.11041265884520551	No Hit
TTCCGCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA	481	0.10860631677820828	No Hit
TTCCGCGGGCTCCTGGCCACTCAGGGAGCTTGGCTTCTTAAGTCTGACTCTT	475	0.10725156022796035	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGTAG	469	0.10589680367771245	No Hit
TTCCGCGGGGGACGCTGTTGAGCTGGGAGGCGCGGTTGGAAGTCGTTACTTT	466	0.1052194254025885	No Hit
TTCCGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	466	0.1052194254025885	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAA	463	0.10454204712746452	No Hit
TTCCGCGGGATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTA	462	0.10431625436908988	No Hit
TTCCGCGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG	454	0.10250991230209267	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT	445	0.10047777747672078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.006322197234490295	0.0	0.0	0.0	0.0
8	0.014902322052727126	0.0	0.0	0.0	0.0
9	0.02528878893796118	0.0	0.0	0.0	0.0
10	0.0731568537133877	0.0	0.0	0.0	0.0
11	0.20140714047019084	0.0	0.0	0.0	0.0
12	0.2142773276975461	0.0	0.0	0.0	0.0
13	0.2704997245328348	0.0	0.0	0.0	0.0
14	0.46987473017765374	0.0	0.0	0.0	0.0
15	0.5351288373479286	0.0	0.0	0.0	0.0
16	0.7891456905194136	0.0	0.0	0.0	0.0
17	0.9033968262569883	0.0	0.0	0.0	0.0
18	0.947200621381671	0.0	0.0	0.0	0.0
19	1.0212606461285574	0.0	0.0	0.0	0.0
20	1.0810957270978405	0.0	0.0	0.0	0.0
21	1.1215126308469034	0.0	0.0	0.0	0.0
22	1.1504141039188591	0.0	0.0	0.0	0.0
23	1.194217899043542	0.0	0.0	0.0	0.0
24	1.265794203448307	0.0	0.0	0.0	0.0
25	1.2946956765202626	0.0	0.0	0.0	0.0
26	1.4870711066554674	0.0	0.0	0.0	0.0
27	1.551873628308993	0.0	0.0	0.0	0.0
28	1.861887085557392	0.0	0.0	0.0	0.0
29	1.8955302065552153	0.0	0.0	0.0	0.0
30	2.0212967729698974	0.0	0.0	0.0	0.0
31	2.04229549949874	0.0	0.0	0.0	0.0
32	2.1350963231907225	0.0	0.0	0.0	0.0
33	2.184319144516397	0.0	0.0	0.0	0.0
34	2.2204459858563417	0.0	0.0	0.0	0.0
35	2.4588831386999757	0.0	0.0	0.0	0.0
36	2.4927520524561735	0.0	0.0	0.0	0.0
37	2.561393051002068	0.0	0.0	0.0	0.0
38	2.618970204387605	0.0	0.0	0.0	0.0
39	2.665031927096034	0.0	0.0	0.0	0.0
40	2.692578643617742	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATTA	20	6.307231E-4	46.000004	38
TATTTCG	35	1.01767E-7	46.000004	19
AACCGTA	20	6.307231E-4	46.000004	41
ACACGTT	20	6.307231E-4	46.000004	40
CGAACCA	20	6.307231E-4	46.000004	15
GTACATA	20	6.307231E-4	46.000004	12
TTAATGC	20	6.307231E-4	46.000004	26
GTATTAG	20	6.307231E-4	46.000004	28
CCGATCA	20	6.307231E-4	46.000004	29
TTAGCGA	20	6.307231E-4	46.000004	27
TCATTCG	20	6.307231E-4	46.000004	27
TCCGAAT	20	6.307231E-4	46.000004	15
TTTCGAC	20	6.307231E-4	46.000004	20
ATAATAG	20	6.307231E-4	46.000004	33
CGGTTCA	20	6.307231E-4	46.000004	42
TCTTATA	20	6.307231E-4	46.000004	18
TTCACTA	20	6.307231E-4	46.000004	26
CCGGATA	55	1.8189894E-12	46.000004	20
ATAGCGC	20	6.307231E-4	46.000004	37
ATTAAGC	20	6.307231E-4	46.000004	22
>>END_MODULE