FastQCFastQC Report
Fri 17 Jun 2016
SRR1527861_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527861_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3121914
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAA682092.184845578705884No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAA267000.8552445711188713No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA204600.6553671882056968No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAA181070.579996758398854No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAA133460.42749415903192717No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATTGTGGGA126560.4053923330367204No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGTAAAAAAAAAAAAAAAAAAA122370.39197107928021074No Hit
TATTGTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA109590.3510346537412626Illumina Single End Adapter 2 (100% over 34bp)
TATTGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA101200.3241601145963662Illumina Single End Adapter 2 (97% over 34bp)
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC97180.31128339858176746No Hit
TATTGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA88130.2822947717329818Illumina Single End Adapter 2 (97% over 34bp)
TATTGTGGGGAGTGCAGTGCTTATTGTGGGGAAAAAAAAAAAAAAAAAAA87090.27896348201776217No Hit
TATTGTGGGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA72770.23309418516973884No Hit
TATTGTGGGAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAA71070.22764880775062987No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGCAAAAAAAAAAAAAAAAAAA68010.21784712839623385No Hit
TATTGTGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAA64380.20621964602484244No Hit
TATTGTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA60160.19270229737270148Illumina Single End Adapter 2 (100% over 34bp)
TATTGTGGGGAGTGCAGTGCTTATTGGGGGAAAAAAAAAAAAAAAAAAAA59780.19148509536137126No Hit
TATTGTGGGACGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAA54860.1757255324778325No Hit
TATTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC51350.16448242968896645No Hit
TATTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC49630.15897298900610332No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT47970.15365573811450284No Hit
TATTGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46500.14894708822856748No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGTAAAAAAAAAAAAAAAAAAAA44280.14183606595184878No Hit
TATTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42270.1353977079445494No Hit
TATTGTGGGGAGTGCAGTGCTTATTGGGGAAAAAAAAAAAAAAAAAAAAA42040.13466098041137584No Hit
TATTGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG39920.12787027445342825No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT38280.122617086825582No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG38110.12207254908367111No Hit
TATTGTGGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAA37990.12168816950114576No Hit
TATTGTGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA36300.11627482371391396Illumina Single End Adapter 2 (100% over 34bp)
TATTGTGGGGAGTGCAGTGCTTATTGTGGGGAGTGCAGTGCTTATTGTGG35110.11246305952053771No Hit
TATTGTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC34700.11114976261357616No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGGAAAAAAAAAAAAAAAAAAAA32750.10490359439753946No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAG351.4471516E-744.021
CCGAATA408.3200575E-944.023
CGCAATT207.8585616E-444.027
TGGGGCT103650.039.2030876
TGGGATT48750.039.0358966
GTGGGGG336100.038.8485575
GTGGGGC235850.038.692395
GTGGGGT336000.037.8976175
TGTGGGG1582700.037.7976884
GTGGGAT157000.037.7923555
CGACTAT357.294224E-637.71428714
TATCGGG357.294224E-637.71428736
TGGGGAG441400.037.6053476
TGGGGTT35800.037.5474856
TGGGGTC41400.037.410636
TGGGGTG223350.036.7503936
TACGGTT301.301607E-436.66666820
GTGGGAC116000.036.6224145
TGGGGAC48650.036.538546
GTGGGAG379750.036.4107975
Produced by FastQC (version 0.11.5)