##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527861_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3121914
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95257909090385	33.0	31.0	34.0	30.0	34.0
2	31.833776010485874	31.0	31.0	34.0	30.0	34.0
3	32.721599634070635	34.0	31.0	34.0	31.0	34.0
4	36.41274967856258	37.0	37.0	37.0	35.0	37.0
5	36.16710101559492	37.0	37.0	37.0	35.0	37.0
6	35.12661879859599	35.0	35.0	37.0	33.0	37.0
7	36.28095745110211	37.0	35.0	37.0	35.0	37.0
8	36.60885149302639	37.0	37.0	37.0	35.0	37.0
9	38.62941259752831	39.0	39.0	39.0	38.0	39.0
10	37.98763034471801	39.0	38.0	39.0	35.0	39.0
11	37.65906652137119	39.0	37.0	39.0	35.0	39.0
12	37.496797797761246	39.0	37.0	39.0	35.0	39.0
13	37.11547339228435	39.0	37.0	39.0	34.0	39.0
14	38.16983299347772	40.0	38.0	40.0	34.0	40.0
15	38.22701490175578	40.0	38.0	40.0	35.0	40.0
16	38.1977072398535	40.0	38.0	40.0	35.0	40.0
17	38.24322034495505	40.0	38.0	40.0	35.0	40.0
18	38.01829102275079	40.0	38.0	40.0	34.0	40.0
19	38.14256927000552	40.0	38.0	40.0	34.0	40.0
20	38.101586718916664	40.0	38.0	40.0	34.0	40.0
21	38.11942897850485	40.0	38.0	40.0	34.0	40.0
22	38.23981378090492	40.0	38.0	40.0	34.0	40.0
23	38.08345553400895	40.0	38.0	40.0	34.0	40.0
24	37.9034637084814	39.0	38.0	40.0	34.0	40.0
25	37.551296416236966	39.0	37.0	40.0	34.0	40.0
26	37.019435512957756	39.0	35.0	40.0	33.0	40.0
27	36.740610407589706	38.0	35.0	40.0	33.0	40.0
28	36.615387227194596	39.0	35.0	40.0	32.0	40.0
29	36.56831834573278	39.0	35.0	40.0	31.0	40.0
30	36.53478891474909	39.0	35.0	40.0	31.0	40.0
31	36.34384579459908	39.0	35.0	40.0	31.0	40.0
32	36.201490495894504	39.0	35.0	40.0	30.0	40.0
33	35.99085817226227	39.0	35.0	40.0	30.0	40.0
34	35.548036557060826	39.0	35.0	40.0	25.0	40.0
35	35.33368984539612	39.0	35.0	40.0	24.0	40.0
36	34.96549648709093	39.0	35.0	40.0	21.0	40.0
37	34.49033189255053	39.0	35.0	40.0	17.0	40.0
38	34.08434537274249	39.0	34.0	40.0	15.0	40.0
39	33.63004330035997	39.0	34.0	40.0	10.0	40.0
40	33.14024921890866	38.0	33.0	40.0	10.0	40.0
41	32.77870754927906	37.0	33.0	40.0	10.0	40.0
42	32.41009137343309	36.0	33.0	40.0	9.0	40.0
43	32.13212471579935	35.0	32.0	40.0	9.0	40.0
44	31.75162320294537	35.0	31.0	40.0	9.0	40.0
45	31.57854060041372	35.0	31.0	40.0	9.0	40.0
46	31.36578329832276	35.0	31.0	40.0	9.0	40.0
47	31.186044522687045	35.0	30.0	39.0	9.0	40.0
48	30.9761815988525	35.0	30.0	39.0	8.0	40.0
49	30.730075203865322	35.0	28.0	39.0	8.0	40.0
50	29.920262377503033	35.0	26.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	13.0
12	29.0
13	32.0
14	37.0
15	52.0
16	176.0
17	396.0
18	852.0
19	1909.0
20	3706.0
21	6611.0
22	10925.0
23	17981.0
24	28562.0
25	41883.0
26	56222.0
27	75882.0
28	99730.0
29	112056.0
30	97630.0
31	77283.0
32	79888.0
33	94169.0
34	153968.0
35	193665.0
36	302883.0
37	607646.0
38	809702.0
39	248023.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.45494975197907	0.29190426129611513	0.19148509536137126	0.061660891363439226
8	98.17387666668589	1.281969970985748	0.311731841428047	0.23242152090031948
9	94.90309470408218	2.7769823255861628	1.1148929791147355	1.2050299912169267
10	46.135800025240925	34.5583190312097	9.180842265353881	10.12503867819549
11	26.156389958211534	44.47797088580916	16.71397738694916	12.651661769030154
12	36.673591905478496	30.3717206816075	18.437727624784024	14.516959788129974
13	21.498926620015798	31.323604686099614	26.35248760856321	20.824981085321376
14	29.09952035834427	33.99225603267739	18.59804594232897	18.31017766664937
15	24.17122957262756	32.684628724558074	19.11676618894691	24.027375513867455
16	24.98925338750523	39.342595600006916	20.764473332705514	14.90367767978234
17	32.49977417699527	31.74661441666875	19.16564645919138	16.587964947144602
18	24.818524789600225	33.34367955042964	24.92192289729954	16.915872762670592
19	29.562313375704775	30.742518852216943	21.400173098938662	18.29499467313962
20	20.118555475903566	31.803246341827478	24.255376669568733	23.82282151270022
21	21.945063188800205	32.62396081378283	30.632041753872784	14.798934243544185
22	20.06692048531766	31.9567419217826	30.450806780712085	17.52553081218765
23	21.61299126113019	38.287761930661766	25.800550559688705	14.298696248519338
24	24.030386487263904	30.448724724640076	31.26511492629201	14.255773861804007
25	24.722173640913876	29.94928752041216	28.174446829733295	17.15409200894067
26	32.15197471807359	28.94166847645387	23.983876557778338	14.922480247694203
27	27.908135842306997	28.673051211532414	26.506431631364606	16.912381314795986
28	35.54643081135483	32.19470491499766	16.88608334502488	15.372780928622634
29	34.60566178312407	31.85548993341905	18.412582793760492	15.126265489696387
30	31.873459678902112	34.85294598121537	17.211140345313805	16.062453994568717
31	27.7628723917443	39.55733566011107	16.734862010933035	15.944929937211597
32	25.87854758330947	41.649834044115245	17.662626196621687	14.808992175953598
33	25.6271633363379	42.14738138206241	17.34512866145576	14.880326620143924
34	23.2872846593468	43.452446159631556	17.934318498203346	15.325950682818295
35	22.65132864005863	45.163800155929984	17.690685906146037	14.494185297865346
36	23.34417283756055	43.85015090101777	17.244261052674737	15.561415208746944
37	24.284941865791307	43.45651417687995	17.073628549665365	15.184915407663377
38	25.061388622492487	42.05666780058643	16.86090648236947	16.021037094551613
39	24.731590940685745	40.21696305535642	17.20156288738255	17.84988311657528
40	23.99569622994099	39.585107084948525	18.74769772645883	17.671498958651647
41	23.449909254386892	39.08845663269392	19.669728250041484	17.79190586287771
42	23.50926386825518	39.23631464543866	18.90791994910814	18.346501537198016
43	23.275368892288512	38.46588983553038	20.344090195950304	17.9146510762308
44	23.602251695594433	38.66621566128984	19.797278208176138	17.934254434939593
45	24.237695208772568	38.816411983161615	19.062696794338347	17.883196013727478
46	23.618651891115512	39.497756824819646	19.033772230753314	17.849819053311528
47	23.8789729633808	39.082306559373514	18.81230552795497	18.226414949290724
48	24.1710373828363	38.63434418757211	19.00484766716828	18.189770762423308
49	24.00469711849846	38.10973012068878	19.282401757383454	18.603171003429306
50	22.128892724142947	38.62444641332208	19.793562538878394	19.45309832365658
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	116.5
2	233.0
3	288.5
4	344.0
5	996.0
6	1648.0
7	15711.0
8	29774.0
9	22269.5
10	14765.0
11	13112.0
12	11459.0
13	12534.0
14	13609.0
15	16803.5
16	19998.0
17	23689.0
18	27380.0
19	33441.5
20	39503.0
21	48848.5
22	58194.0
23	71462.5
24	84731.0
25	97000.0
26	109269.0
27	139911.0
28	170553.0
29	193586.5
30	216620.0
31	182835.5
32	149051.0
33	128759.0
34	108467.0
35	110316.5
36	112166.0
37	107149.5
38	102133.0
39	96709.0
40	91285.0
41	93589.0
42	95893.0
43	106491.0
44	117089.0
45	121771.5
46	126454.0
47	149756.5
48	173059.0
49	187358.5
50	201658.0
51	192958.0
52	184258.0
53	168906.5
54	153555.0
55	147245.0
56	140935.0
57	138548.0
58	136161.0
59	129916.5
60	123672.0
61	112330.0
62	100988.0
63	82630.0
64	64272.0
65	55348.5
66	46425.0
67	40308.0
68	34191.0
69	28261.5
70	22332.0
71	23574.0
72	24816.0
73	16859.0
74	8902.0
75	6265.5
76	3629.0
77	2585.5
78	1542.0
79	1056.5
80	571.0
81	418.0
82	265.0
83	152.5
84	40.0
85	28.0
86	16.0
87	9.5
88	3.0
89	4.5
90	6.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3121914.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.355678669259728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.69215720260517	16.921495442045345
2	10.217404652648602	4.568340304968229
3	3.8285581091304586	2.5677004456292747
4	1.973457851305311	1.764719583644371
5	1.1426569314879689	1.2772435594773681
6	0.7746239027138138	1.0390345835158756
7	0.5623686299016617	0.8800492668627379
8	0.47751722340903396	0.8540177284455578
9	0.3719650681389915	0.7483978385554134
>10	3.601942833579306	18.96851687902768
>50	0.8736177262023158	13.36315156396547
>100	0.4391424178284196	17.563115888632897
>500	0.026840472177538103	4.129303607743058
>1k	0.014813593934051085	6.492990230114593
>5k	0.001613361715589722	2.5404188232174807
>10k+	0.0013200232218461363	6.321504254154611
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAA	68209	2.184845578705884	No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAA	26700	0.8552445711188713	No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20460	0.6553671882056968	No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAA	18107	0.579996758398854	No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAA	13346	0.42749415903192717	No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATTGTGGGA	12656	0.4053923330367204	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGTAAAAAAAAAAAAAAAAAAA	12237	0.39197107928021074	No Hit
TATTGTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	10959	0.3510346537412626	Illumina Single End Adapter 2 (100% over 34bp)
TATTGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	10120	0.3241601145963662	Illumina Single End Adapter 2 (97% over 34bp)
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	9718	0.31128339858176746	No Hit
TATTGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	8813	0.2822947717329818	Illumina Single End Adapter 2 (97% over 34bp)
TATTGTGGGGAGTGCAGTGCTTATTGTGGGGAAAAAAAAAAAAAAAAAAA	8709	0.27896348201776217	No Hit
TATTGTGGGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA	7277	0.23309418516973884	No Hit
TATTGTGGGAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAA	7107	0.22764880775062987	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGCAAAAAAAAAAAAAAAAAAA	6801	0.21784712839623385	No Hit
TATTGTGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAA	6438	0.20621964602484244	No Hit
TATTGTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	6016	0.19270229737270148	Illumina Single End Adapter 2 (100% over 34bp)
TATTGTGGGGAGTGCAGTGCTTATTGGGGGAAAAAAAAAAAAAAAAAAAA	5978	0.19148509536137126	No Hit
TATTGTGGGACGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAA	5486	0.1757255324778325	No Hit
TATTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	5135	0.16448242968896645	No Hit
TATTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4963	0.15897298900610332	No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4797	0.15365573811450284	No Hit
TATTGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4650	0.14894708822856748	No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGTAAAAAAAAAAAAAAAAAAAA	4428	0.14183606595184878	No Hit
TATTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4227	0.1353977079445494	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGGGGAAAAAAAAAAAAAAAAAAAAA	4204	0.13466098041137584	No Hit
TATTGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	3992	0.12787027445342825	No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	3828	0.122617086825582	No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	3811	0.12207254908367111	No Hit
TATTGTGGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAA	3799	0.12168816950114576	No Hit
TATTGTGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	3630	0.11627482371391396	Illumina Single End Adapter 2 (100% over 34bp)
TATTGTGGGGAGTGCAGTGCTTATTGTGGGGAGTGCAGTGCTTATTGTGG	3511	0.11246305952053771	No Hit
TATTGTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC	3470	0.11114976261357616	No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGGAAAAAAAAAAAAAAAAAAAA	3275	0.10490359439753946	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.016047847570432754	0.0	0.0	0.0	0.0
8	0.16928717447053315	0.0	0.0	0.0	0.0
9	0.23604109530243306	0.0	0.0	0.0	0.0
10	0.6855730170658129	0.0	0.0	0.0	0.0
11	1.1475332119975117	0.0	0.0	0.0	0.0
12	1.2810730852931886	0.0	0.0	0.0	0.0
13	1.4044909629156985	0.0	0.0	0.0	0.0
14	1.517754813233164	0.0	0.0	0.0	0.0
15	1.6574127282173692	0.0	0.0	0.0	0.0
16	1.832657786217045	0.0	0.0	0.0	0.0
17	1.9537053230806487	0.0	0.0	0.0	0.0
18	2.0765145996974934	0.0	0.0	0.0	0.0
19	2.1972738518742028	0.0	0.0	0.0	0.0
20	2.33779662091909	0.0	0.0	0.0	3.203163187711128E-5
21	2.4865194877245176	0.0	0.0	0.0	3.203163187711128E-5
22	2.660451248817232	0.0	0.0	0.0	3.203163187711128E-5
23	2.8439604678412027	0.0	0.0	0.0	3.203163187711128E-5
24	3.029199394986537	0.0	0.0	0.0	3.203163187711128E-5
25	3.202746776496726	0.0	0.0	0.0	3.203163187711128E-5
26	3.378888720188961	0.0	0.0	0.0	3.203163187711128E-5
27	3.535395273540527	0.0	0.0	0.0	3.203163187711128E-5
28	3.7166622783330996	0.0	0.0	0.0	3.203163187711128E-5
29	3.875090729597292	0.0	0.0	0.0	3.203163187711128E-5
30	4.043481018375266	3.203163187711128E-5	0.0	0.0	3.203163187711128E-5
31	4.219719056963132	3.203163187711128E-5	0.0	0.0	6.406326375422256E-5
32	4.382760063217629	3.203163187711128E-5	0.0	0.0	6.406326375422256E-5
33	4.520560143552961	3.203163187711128E-5	0.0	0.0	6.406326375422256E-5
34	4.656214104552528	3.203163187711128E-5	0.0	0.0	6.406326375422256E-5
35	4.807307312116862	3.203163187711128E-5	0.0	0.0	6.406326375422256E-5
36	4.932903340707015	3.203163187711128E-5	0.0	0.0	6.406326375422256E-5
37	5.065674454837641	3.203163187711128E-5	0.0	0.0	6.406326375422256E-5
38	5.202353428057275	3.203163187711128E-5	0.0	0.0	6.406326375422256E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATAG	35	1.4471516E-7	44.0	21
CCGAATA	40	8.3200575E-9	44.0	23
CGCAATT	20	7.8585616E-4	44.0	27
TGGGGCT	10365	0.0	39.203087	6
TGGGATT	4875	0.0	39.035896	6
GTGGGGG	33610	0.0	38.848557	5
GTGGGGC	23585	0.0	38.69239	5
GTGGGGT	33600	0.0	37.897617	5
TGTGGGG	158270	0.0	37.797688	4
GTGGGAT	15700	0.0	37.792355	5
CGACTAT	35	7.294224E-6	37.714287	14
TATCGGG	35	7.294224E-6	37.714287	36
TGGGGAG	44140	0.0	37.605347	6
TGGGGTT	3580	0.0	37.547485	6
TGGGGTC	4140	0.0	37.41063	6
TGGGGTG	22335	0.0	36.750393	6
TACGGTT	30	1.301607E-4	36.666668	20
GTGGGAC	11600	0.0	36.622414	5
TGGGGAC	4865	0.0	36.53854	6
GTGGGAG	37975	0.0	36.410797	5
>>END_MODULE