FastQCFastQC Report
Fri 17 Jun 2016
SRR1527860_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527860_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4551186
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAA912602.00519161379034No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAA355150.7803460460635975No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA264170.5804421089360005No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA234300.514810864684502No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAA184150.40461980679321835No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATTGTGGGAAG166690.36625618025719014No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGTAAAAAAAAAAAAAAAAAAAAA163740.3597743533224087No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC149060.32751902471136096No Hit
TATTGTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA122660.269512166718741Illumina Single End Adapter 2 (100% over 34bp)
TATTGTGGGGAGTGCAGTGCTTATTGTGGGGAAAAAAAAAAAAAAAAAAAAA116510.25599920548182387No Hit
TATTGTGGGAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAA95410.20963766367711623No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGCAAAAAAAAAAAAAAAAAAAAA95020.20878074418404347No Hit
TATTGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA92750.2037930332884659Illumina Single End Adapter 2 (97% over 34bp)
TATTGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA90840.19959632500187863Illumina Single End Adapter 2 (97% over 34bp)
TATTGTGGGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA85410.18766536898294203No Hit
TATTGTGGGGAGTGCAGTGCTTATTGGGGGAAAAAAAAAAAAAAAAAAAAAA80450.17676711081463162No Hit
TATTGTGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA79720.1751631333019569No Hit
TATTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC78950.1734712666105055No Hit
TATTGTGGGACGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAA76630.16837369424145707No Hit
TATTGTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA72440.15916730276459806Illumina Single End Adapter 2 (100% over 34bp)
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG72030.1582664386821369No Hit
TATTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT71050.15611315380210786No Hit
TATTGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60070.13198757422790455No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGTAAAAAAAAAAAAAAAAAAAAAA58390.12829622871928328No Hit
TATTGTGGGGAGTGCAGTGCTTATTGGGGAAAAAAAAAAAAAAAAAAAAAAA57100.1254618027037348No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC57030.12530799664087558No Hit
TATTGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC57020.1252860243461814No Hit
TATTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56350.12381388060167173No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT53630.11783741644485635No Hit
TATTGTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT52750.11590385451176902No Hit
TATTGTGGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAA50760.11153136786762835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATT206.3134334E-446.027
CGATATA206.3134334E-446.031
TGGGATT72400.040.599456
GTGGGGG510600.040.4954955
GTGGGGC352800.040.2825935
TGGGGCT159800.040.24286
GTGGGGT500850.039.525015
TCGTTAA1050.039.4285738
TCGATAG700.039.4285743
ACGATAT355.3766216E-639.4285738
CGAATAG700.039.4285724
CCGAATA700.039.4285723
TGTGGGG2338400.039.37564
GTGGGAT225200.038.9937865
TGGGGTC67500.038.5718546
TGGGGAG651700.038.4403846
TGGGGTT53850.038.4401136
ATATCGC301.0020327E-438.33333210
CCGCAAT601.2369128E-1038.33333231
TGGGGTG330500.038.310146
Produced by FastQC (version 0.11.5)