##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527860_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4551186
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67761084693089	31.0	31.0	33.0	30.0	34.0
2	31.478203703386328	31.0	31.0	34.0	30.0	34.0
3	32.39706419381673	34.0	31.0	34.0	30.0	34.0
4	36.25666606462579	37.0	35.0	37.0	35.0	37.0
5	35.886359072118786	37.0	35.0	37.0	35.0	37.0
6	34.91429552648474	35.0	35.0	37.0	33.0	37.0
7	36.20629809460655	37.0	35.0	37.0	35.0	37.0
8	36.539534969566176	37.0	37.0	37.0	35.0	37.0
9	38.559545138344156	39.0	39.0	39.0	37.0	39.0
10	37.73117754361171	39.0	38.0	39.0	35.0	39.0
11	37.331553577463104	39.0	37.0	39.0	34.0	39.0
12	37.208419959105164	39.0	37.0	39.0	34.0	39.0
13	36.8210607081319	39.0	37.0	39.0	33.0	39.0
14	37.86509604309734	40.0	37.0	40.0	33.0	40.0
15	37.953122328992926	40.0	38.0	40.0	34.0	40.0
16	37.930225440138024	40.0	38.0	40.0	34.0	40.0
17	37.97522074465864	40.0	38.0	40.0	34.0	40.0
18	37.84563342390313	40.0	38.0	40.0	33.0	40.0
19	37.94684418522996	40.0	38.0	40.0	34.0	40.0
20	37.999752591961744	40.0	38.0	40.0	34.0	40.0
21	37.996553645577215	40.0	38.0	40.0	34.0	40.0
22	37.994403656541394	40.0	38.0	40.0	34.0	40.0
23	37.815344395944265	40.0	38.0	40.0	33.0	40.0
24	37.662427991297214	39.0	37.0	40.0	33.0	40.0
25	37.29818623101759	39.0	37.0	40.0	33.0	40.0
26	36.70811937811375	38.0	35.0	40.0	32.0	40.0
27	36.41234218948643	38.0	35.0	40.0	31.0	40.0
28	36.37931783056109	38.0	35.0	40.0	31.0	40.0
29	36.27060418976504	38.0	35.0	40.0	31.0	40.0
30	36.012939704068344	38.0	35.0	40.0	30.0	40.0
31	35.66571680436704	38.0	35.0	40.0	29.0	40.0
32	35.43346855083488	38.0	35.0	40.0	27.0	40.0
33	35.25012930695428	38.0	35.0	40.0	26.0	40.0
34	34.99105288160053	38.0	35.0	40.0	24.0	40.0
35	34.72975857282036	38.0	34.0	40.0	23.0	40.0
36	34.40174407286364	38.0	34.0	40.0	20.0	40.0
37	33.917435147673594	38.0	33.0	40.0	15.0	40.0
38	33.556644575721585	38.0	33.0	40.0	15.0	40.0
39	33.09797028730533	38.0	33.0	40.0	10.0	40.0
40	32.619077752480344	37.0	32.0	40.0	10.0	40.0
41	32.26236216230231	37.0	32.0	40.0	10.0	40.0
42	31.860208525865566	35.0	31.0	40.0	9.0	40.0
43	31.569713916328624	35.0	31.0	39.0	9.0	40.0
44	31.341866493700763	35.0	30.0	39.0	9.0	40.0
45	31.077772914576553	35.0	30.0	39.0	9.0	40.0
46	30.867156165447863	35.0	29.0	39.0	9.0	40.0
47	30.61543957992488	35.0	28.0	38.0	9.0	40.0
48	30.36937273053661	35.0	27.0	38.0	8.0	40.0
49	30.348487405260958	35.0	27.0	38.0	8.0	40.0
50	30.114167603785035	35.0	26.0	38.0	8.0	40.0
51	29.905200314819037	35.0	25.0	38.0	8.0	40.0
52	29.22829499827078	35.0	23.0	37.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	11.0
12	15.0
13	22.0
14	18.0
15	60.0
16	226.0
17	716.0
18	1926.0
19	4227.0
20	8317.0
21	15209.0
22	25548.0
23	41354.0
24	59971.0
25	81595.0
26	109794.0
27	141250.0
28	159008.0
29	146175.0
30	119918.0
31	116973.0
32	140280.0
33	178053.0
34	277474.0
35	344919.0
36	510573.0
37	892402.0
38	934350.0
39	240802.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.49674656232463	0.28364035220709505	0.16472629332222416	0.054886792146047206
8	98.21686039638898	1.2570569517484014	0.3042723369249246	0.22181031493768874
9	94.83066611648042	2.7921952651462716	1.1111169703896961	1.2660216479836246
10	46.317992716623756	34.3155827953417	9.195383357217219	10.17104113081733
11	26.346890678605533	43.8817486255231	16.844158863206207	12.927201832665155
12	36.809877689024354	29.791794929936945	18.600096765985832	14.798230615052868
13	21.500154025785807	30.75385185312136	26.462047475097698	21.283946645995133
14	29.063303499351594	33.43825104049801	18.893031398848564	18.605414061301822
15	24.52505786403808	31.891379521733455	19.464113310244848	24.11944930398362
16	25.093283377124116	38.4975037275998	21.122142667867234	15.287070227408856
17	32.64597403841548	31.101761167308915	19.36249144728429	16.889773346991312
18	25.26310724281539	32.62145295753678	24.962745974346028	17.152693825301803
19	29.613182146368	30.144933650261713	21.6484010980874	18.59348310528289
20	20.344850770766126	31.353827332040485	24.400562842300886	23.900759054892504
21	22.25916497370136	31.905573624105894	30.763893191796598	15.071368210396146
22	20.418963320769574	31.336952609715357	30.32835836636868	17.915725703146386
23	21.879022303197452	37.72249694914688	25.804416694901068	14.594064052754602
24	24.21551217638655	30.12405997030225	31.129753870749294	14.53067398256191
25	24.999351817306522	29.30117556171073	28.102652803027606	17.596819817955144
26	32.29208826007111	28.3033257704695	24.101849495933585	15.302736473525801
27	28.007336988644276	28.118253132260467	26.419465167980388	17.454944711114862
28	35.4949676853462	31.723181605849554	17.010972524524377	15.770878184279876
29	34.60948420917097	31.351520241097596	18.749815982031937	15.289179567699495
30	31.962503839658496	34.33370993846439	17.481443298516037	16.222342923361076
31	28.06771685446387	38.816365668201655	16.96430337059395	16.151614106740528
32	26.213343071454343	40.816943100106215	18.037671938699056	14.932041889740388
33	26.057295834536315	41.287655569339506	17.59853365694129	15.056514939182886
34	23.359427630512133	42.80323854045957	18.164891524978323	15.672442304049977
35	22.804759023252398	44.62500543814294	17.990717144937605	14.579518393667056
36	23.786173538062386	43.2300503648939	17.36055173310869	15.623224363935027
37	24.54235005996239	42.81174181850621	17.34750019006035	15.29840793147105
38	25.264777137212146	41.56920855355066	17.06109132872179	16.10492298051541
39	24.8432826080938	39.77686255846278	17.276222066072446	18.103632767370968
40	23.991812244105162	39.199123041774165	19.007001691427245	17.802063022693424
41	23.450239124483158	38.72555417423063	19.925970944716386	17.898235756569825
42	23.652516069437723	38.76883959477816	18.976460201802343	18.60218413398178
43	23.36751343495959	37.86364697026225	20.640246300634605	18.12859329414355
44	23.640035806051436	38.13884996130679	20.112537698964623	18.108576533677155
45	24.536659235636602	38.22493741191856	19.209476387034062	18.028926965410776
46	23.63915691426367	39.07126625894877	19.24157790958225	18.04799891720532
47	24.18417968415266	38.48976947986745	18.959695340950688	18.366355495029207
48	24.620307761537322	37.99356914878891	19.09192461042023	18.29419847925354
49	24.32946049667054	37.530722761056126	19.41581820650705	18.72399853576628
50	22.118366509301094	38.38764225412892	19.727209566912887	19.766781669657096
51	21.8811096711934	38.309970192385016	20.13897036948171	19.66994976693987
52	22.498223539973978	37.10276837729769	20.710689477424125	19.6883186053042
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	150.5
2	301.0
3	364.5
4	428.0
5	1309.5
6	2191.0
7	20550.0
8	38909.0
9	29199.5
10	19490.0
11	17224.0
12	14958.0
13	15650.0
14	19198.0
15	22054.0
16	25014.0
17	27974.0
18	33405.5
19	38837.0
20	48800.5
21	58764.0
22	77217.0
23	95670.0
24	115891.5
25	136113.0
26	184136.0
27	232159.0
28	268034.0
29	303909.0
30	259264.5
31	214620.0
32	188576.5
33	162533.0
34	161654.0
35	160775.0
36	157004.5
37	153234.0
38	143636.5
39	136141.5
40	138244.0
41	150778.5
42	163313.0
43	158584.0
44	153855.0
45	162306.0
46	170757.0
47	201765.0
48	232773.0
49	259126.0
50	285479.0
51	278561.0
52	271643.0
53	241587.5
54	211532.0
55	214432.5
56	217333.0
57	204765.5
58	192198.0
59	187697.5
60	183197.0
61	163211.0
62	143225.0
63	132502.5
64	96274.5
65	70769.0
66	61280.5
67	51792.0
68	45874.5
69	39957.0
70	40229.5
71	40502.0
72	29242.5
73	17983.0
74	12353.0
75	6723.0
76	4849.0
77	2975.0
78	2068.5
79	1162.0
80	738.0
81	314.0
82	325.0
83	336.0
84	176.5
85	17.0
86	15.0
87	13.0
88	9.5
89	7.0
90	8.0
91	4.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	4551186.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.719091251322904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.47112066924839	18.612636717474736
2	9.277819006748937	4.401228712686721
3	3.6668810608589113	2.6092525947078076
4	1.8225764063566037	1.7291942437952184
5	1.0493735702276172	1.2445093734477677
6	0.6946814823913645	0.9886328082867024
7	0.4831862590916595	0.8022517279546295
8	0.3675159209353432	0.6973714931983503
9	0.28941945488197074	0.6178289814228238
>10	2.51800911340658	13.138134038150293
>50	0.8163647176114718	13.799873263153398
>100	0.49687251198391025	21.383833134315616
>500	0.02852556672237683	4.602668704064787
>1k	0.014773827716139401	6.198079506955828
>5k	0.0019512602643957698	3.2922014882749826
>10k+	9.291715544741761E-4	5.882303212110441
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAA	91260	2.00519161379034	No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAA	35515	0.7803460460635975	No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26417	0.5804421089360005	No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA	23430	0.514810864684502	No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAA	18415	0.40461980679321835	No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATTGTGGGAAG	16669	0.36625618025719014	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGTAAAAAAAAAAAAAAAAAAAAA	16374	0.3597743533224087	No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	14906	0.32751902471136096	No Hit
TATTGTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	12266	0.269512166718741	Illumina Single End Adapter 2 (100% over 34bp)
TATTGTGGGGAGTGCAGTGCTTATTGTGGGGAAAAAAAAAAAAAAAAAAAAA	11651	0.25599920548182387	No Hit
TATTGTGGGAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAA	9541	0.20963766367711623	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGCAAAAAAAAAAAAAAAAAAAAA	9502	0.20878074418404347	No Hit
TATTGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	9275	0.2037930332884659	Illumina Single End Adapter 2 (97% over 34bp)
TATTGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	9084	0.19959632500187863	Illumina Single End Adapter 2 (97% over 34bp)
TATTGTGGGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	8541	0.18766536898294203	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGGGGGAAAAAAAAAAAAAAAAAAAAAA	8045	0.17676711081463162	No Hit
TATTGTGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA	7972	0.1751631333019569	No Hit
TATTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	7895	0.1734712666105055	No Hit
TATTGTGGGACGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAA	7663	0.16837369424145707	No Hit
TATTGTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	7244	0.15916730276459806	Illumina Single End Adapter 2 (100% over 34bp)
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	7203	0.1582664386821369	No Hit
TATTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	7105	0.15611315380210786	No Hit
TATTGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6007	0.13198757422790455	No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGTAAAAAAAAAAAAAAAAAAAAAA	5839	0.12829622871928328	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGGGGAAAAAAAAAAAAAAAAAAAAAAA	5710	0.1254618027037348	No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	5703	0.12530799664087558	No Hit
TATTGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	5702	0.1252860243461814	No Hit
TATTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5635	0.12381388060167173	No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5363	0.11783741644485635	No Hit
TATTGTGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	5275	0.11590385451176902	No Hit
TATTGTGGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAA	5076	0.11153136786762835	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.014194102372436547	0.0	0.0	0.0	0.0
8	0.15907941358582137	0.0	0.0	0.0	0.0
9	0.22482051931079064	0.0	0.0	0.0	0.0
10	0.637899659561266	0.0	0.0	0.0	0.0
11	1.0587130475440907	0.0	0.0	0.0	0.0
12	1.179824335898379	0.0	0.0	0.0	0.0
13	1.2919709280174443	0.0	0.0	0.0	0.0
14	1.3967128568245728	0.0	0.0	0.0	0.0
15	1.520350959068691	0.0	0.0	0.0	0.0
16	1.690702159832624	0.0	0.0	0.0	0.0
17	1.8090449390554462	0.0	0.0	0.0	0.0
18	1.9229273424553512	0.0	0.0	0.0	0.0
19	2.0397540333442756	0.0	0.0	0.0	0.0
20	2.167347148633345	0.0	0.0	0.0	0.0
21	2.298367941894706	0.0	0.0	0.0	0.0
22	2.4469006540273237	0.0	0.0	0.0	0.0
23	2.6011681350751212	0.0	0.0	0.0	0.0
24	2.7578305962445833	0.0	0.0	0.0	0.0
25	2.899947398326502	0.0	0.0	0.0	0.0
26	3.0466564099995033	0.0	0.0	0.0	0.0
27	3.1765785885261555	0.0	0.0	0.0	0.0
28	3.3195523101011473	0.0	0.0	0.0	0.0
29	3.446771896380416	0.0	0.0	0.0	0.0
30	3.5902290084386794	4.394458938834844E-5	0.0	0.0	0.0
31	3.7433319578676856	4.394458938834844E-5	0.0	0.0	0.0
32	3.8905023877292644	4.394458938834844E-5	0.0	0.0	0.0
33	4.0171067497570965	4.394458938834844E-5	0.0	0.0	0.0
34	4.14114035330571	4.394458938834844E-5	0.0	0.0	0.0
35	4.282488125073333	4.394458938834844E-5	0.0	0.0	0.0
36	4.3973592817344755	4.394458938834844E-5	0.0	0.0	0.0
37	4.523106724269234	4.394458938834844E-5	0.0	0.0	0.0
38	4.650106587601561	4.394458938834844E-5	0.0	0.0	0.0
39	4.789257129899767	4.394458938834844E-5	0.0	0.0	2.197229469417422E-5
40	4.942447968507549	4.394458938834844E-5	0.0	0.0	2.197229469417422E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATT	20	6.3134334E-4	46.0	27
CGATATA	20	6.3134334E-4	46.0	31
TGGGATT	7240	0.0	40.59945	6
GTGGGGG	51060	0.0	40.495495	5
GTGGGGC	35280	0.0	40.282593	5
TGGGGCT	15980	0.0	40.2428	6
GTGGGGT	50085	0.0	39.52501	5
TCGTTAA	105	0.0	39.42857	38
TCGATAG	70	0.0	39.42857	43
ACGATAT	35	5.3766216E-6	39.42857	38
CGAATAG	70	0.0	39.42857	24
CCGAATA	70	0.0	39.42857	23
TGTGGGG	233840	0.0	39.3756	4
GTGGGAT	22520	0.0	38.993786	5
TGGGGTC	6750	0.0	38.571854	6
TGGGGAG	65170	0.0	38.440384	6
TGGGGTT	5385	0.0	38.440113	6
ATATCGC	30	1.0020327E-4	38.333332	10
CCGCAAT	60	1.2369128E-10	38.333332	31
TGGGGTG	33050	0.0	38.31014	6
>>END_MODULE